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Fix emboss tool versions #4829

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rogmei opened this issue Oct 4, 2022 · 7 comments
Closed

Fix emboss tool versions #4829

rogmei opened this issue Oct 4, 2022 · 7 comments

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@rogmei
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rogmei commented Oct 4, 2022

The tool sixpack on usegalaxy.eu only acepts fasta files, but throws an error every time I try.
Have tried both single fasta and multifasta, always with the same result.
Under is an example of a fasta that fails in Galaxy. Pasting this same sequence into the EMBOS online sixpack tool works.
Other Galaxy tools like seqret does work.

The Galaxy error:

An error occurred with this dataset:
format sixpack database ?
Died: Value required for '-uppercase' before '-number'

contig_10
TGAAATGAATGATAAGCCAGTTTTGTTGTCAAATAGTTTCTGTGTGGCTTCGGTAGATAA
TATCGATAGGAATAGTGCTTATGCATCAGTTAAAGTTGGTGAGGCTAATAGAGGATTTCA
TGGAACTTCTGTTCAGTCTGTAGAGCCTTTGCCTATTTCAACCTCAGTCGGAAGGACTGC
TTGTAATTCGTTACATACTTCCAGTAGAGTTGGTCAACAAAGCAATTTGTCAGCTGCAGA
TATCAGGGATTATCAACACACTTTTACTCAAACTTCTCATTCAGAAGTTACATCTTATAG

@bernt-matthias
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Thanks for reporting. Which tool version do you use and on which galaxy are you working? Can you tell us which non default parameters values you are using? If you see the executed command line in the dataset info page this would also be of interest.

@rogmei
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rogmei commented Oct 10, 2022 via email

@bernt-matthias
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@rogmei plase share the history or data set and post the link

@rogmei
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rogmei commented Oct 10, 2022

https://usegalaxy.eu/u/8b383e1220ee48ae84c86849cc93bdf9/h/sixpack-problem
Here is an example history using a single fasta file as input.

@bernt-matthias
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Seems that we messed up the versions. 5.0.0.1 is not the latest version, but 5.0.0+galaxy2 as a workaround you can manually switch the version on the top of the tool form (the button with the 3 boxes on the top right.

I guess to fix this we need to readd .1 to the tool versions

@bernt-matthias bernt-matthias changed the title Sixpack tool not working Fix emboss tool versions Oct 10, 2022
bernt-matthias added a commit to bernt-matthias/tools-iuc that referenced this issue Oct 10, 2022
at some time in the past we changed from 5.0.0.1 to 5.0.0+galaxy2
which was wrong since `"+" < "." == True`.

fixes galaxyproject#4829
@rogmei
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rogmei commented Oct 10, 2022

Thanks for solving this.
Switching versions works.
Would never have guessed this had to do with a version conflict.

@rogmei
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rogmei commented Oct 10, 2022

Solved

@rogmei rogmei closed this as completed Oct 10, 2022
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