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STAR 2.7.4a ______ 2020/06/01

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@alexdobin alexdobin released this 01 Jun 19:12
· 337 commits to master since this release

Fixed multiple bugs and issues.

This version requires re-generation of the genome indexes

  • Fixed the long-standing seg-fault problem for small genomes.
  • Issue #784: Fixed a seg-fault in STARsolo for cases where no cell barcodes matched whitelist.
  • Issue #798: Fixed the problem in Solo Q30 Bases in Summary.csv average (#798).
  • Issue #843, #880: Throw an error if read file in --readFilesIn does not exist when using --readFilesCommand .
  • Issue #864: Fixed seg-fault for STARsolo runs with very small number of reads or cells.
  • Issue #881: Check if --genomeDir exists, create if necessary.
  • Issue #882: Added 3rd column "Gene Expression" to solo features.tsv file for better compatibility with downstream tools.
  • Issue #902: Fixed seg-fault for STARsolo CB/UB SAM attributes output with --soloFeatures GeneFull only option.
  • Issue #907: Fixed the bug that prevented output of STARsolo GX/GN tags into the Aligned.out.bam if --quantMode TranscriptomeSAM is used.
  • Issue #910: The output directory in --outFileNamePrefix is checked and created if it does not exist.
  • If solo barcode read length is not checked (--soloBarcodeReadLength 0) and it is shorter than CB+UMI length, the barcode is padded with Ns and not counted.
  • For genome generation runs, the Log.out file is moved into the --genomeDir directory.
  • Fixed a bug with solo SJ output for large genomes.
  • Implemented --seedMapMin option (previously hard-coded) to define minimum seed length.