Experimental References

Experimental and in-development sequence references

Reference sequences used in experiments and their associated files are stored in the working directory: s3://serratus-public/seq/

CoV Pan-Genome Series

• ~/seq/cov0 : All CoV sequences from NCBI

  • NCBI search: "(Coronaviridae) AND "viruses"[porgn:txid10239]"
  • Date Accessed: 2020/03/30
  • Results: 33296

• ~/seq/cov1r : Initial pan-coronavirus genome

  • Based off of cov0 with non-CoV accessions removed and polyA[10+] masked
  • See: ~/serratus/notebook/200408_cov1_pangenome.ipynb for make commands
  • Date: 2020/04/08
  • cov01r contains reverse non-compliment control sequences

• ~/seq/cov2r : Refined pan-coronavirus genome

  • Based off of cov0
  • Removed poly-nt tracts of 10+
  • Blacklisted 6 non-CoV accessions
  • Pruned
  • See: ~/serratus/notebook/200420_cov2_pangenome.ipynb notebook for commands
  • Date: 2020/04/20
  • cov2.fa : Masked pan-genome
  • cov2r.fa : Masked pan-genome with reverse non-compliment controls

• ~/seq/cov2m : Pan-coronavirus genome + mega0 (see flom1)

  • Based off of cov0
    • Removed poly-nt tracts of 10+
  • Blacklisted 6 non-CoV accessions + additional false-positive regions
  • See: ~/serratus/notebook/200509_cov2m_CoVpan_and_mega_genome.ipynb notebook for generation script
  • Date: 2020/05/09
  • cov2m.fa : Hard masked pan-genome
  • cov2m.unmasked.fa : Unmasked pan-genome

• ~/seq/cov3 : Pan-Coronavirus genome

  • Major revision from cov2
    • CovRef2 : RefSeq representative genomes for CoV
    • cov0 :
    • Removed poly-nt tracts of 10+
  • Blacklisted 6 non-CoV accessions + additional false-positive regions
  • See: `` notebook for generation script
  • Date: 2020/05/09

FLOM Series

See: (Full Length Only Mega-Reference](https://github.com/ababaian/serratus/wiki/FLOM-reference)

Other

• ~/seq/hgr1 : Human rDNA testing sequence
• From this publication