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TheBGAcademy 2024: Annotation best practise with EASEL and EnTAP

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Easel and Entap

This session is part of Biodiversity Genomics Academy 2024

Open in Gitpod

YouTube Recording <-- To be updated after BGA24

Session Leader(s)

  • Cynthia Webster - PhD Student @ University of Connecticut

  • Alex Hart - Research Assistant @ University of Connecticut

Description

Introduction:

  • What is genome annotation?
  • Current challenges
  • Best practices
    • Introduction to functional annotation with EnTAP
    • Introduction to structural and functional annotation with EASEL

Running EnTAP as a standalone tool:

  • Installing dependencies
  • Setting parameters and executing

Running EASEL:

  • Installing dependencies
  • Setting parameters and executing

Annotating Juglans cinerea with EASEL and EnTAP:

  • Evidence generation
  • Gene prediction
  • Secondary and primary feature filtering
  • Evaluation
  • Benchmarking against commonly used annotation tools

By the end of this session you will be able to: (1) Structurally annotate a eukaryotic genome assembly with protein and RNA extrinsic evidence using the EASEL nextflow pipeline, (2) Assess non-model structural genome annotations with comprehensive metrics that include providing functional annotation with EnTAP, (3) Address challenges with accuracy and efficiency with structural and functional genome annotation.

Prerequisites

  1. Understanding of linux command line basics

[!warning] "Please make sure you MEET THE PREREQUISITES and READ THE DESCRIPTION above"

You will get the most out of this session if you meet the prerequisites above.

Please also read the description carefully to see if this session is relevant to you.

If you don't meet the prerequisites or change your mind based on the description or are no longer available at the session time, please email damon at thebgacademy.org to cancel your slot so that someone else on the waitlist might attend.

Instructions

For Session Leader

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TheBGAcademy 2024: Annotation best practise with EASEL and EnTAP

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