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Add support for reading and writing splitting BAM index files. (#1138)
* Add support for reading and writing SBI files for BAMs. * SBI files are splitting bam indexes which are used to seek to a a specific record number in a bam. This is different than the existing bai index which is used to query by genomic coordinates and is useful for reading bams in a distributed parallel system like apache-spark.
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/* | ||
* The MIT License | ||
* | ||
* Copyright (c) 2018 The Broad Institute | ||
* | ||
* Permission is hereby granted, free of charge, to any person obtaining a copy | ||
* of this software and associated documentation files (the "Software"), to deal | ||
* in the Software without restriction, including without limitation the rights | ||
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell | ||
* copies of the Software, and to permit persons to whom the Software is | ||
* furnished to do so, subject to the following conditions: | ||
* | ||
* The above copyright notice and this permission notice shall be included in | ||
* all copies or substantial portions of the Software. | ||
* | ||
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR | ||
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, | ||
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE | ||
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER | ||
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, | ||
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN | ||
* THE SOFTWARE. | ||
*/ | ||
package htsjdk.samtools; | ||
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import htsjdk.samtools.cram.io.InputStreamUtils; | ||
import htsjdk.samtools.seekablestream.SeekablePathStream; | ||
import htsjdk.samtools.seekablestream.SeekableStream; | ||
import htsjdk.samtools.util.BlockCompressedInputStream; | ||
import htsjdk.samtools.util.IOUtil; | ||
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import java.io.EOFException; | ||
import java.io.IOException; | ||
import java.io.OutputStream; | ||
import java.nio.ByteBuffer; | ||
import java.nio.ByteOrder; | ||
import java.nio.file.Files; | ||
import java.nio.file.Path; | ||
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/** | ||
* Writes SBI files for BAM files, as understood by {@link SBIIndex}. | ||
*/ | ||
public final class BAMSBIIndexer { | ||
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/** | ||
* Perform indexing on the given BAM file, at the granularity level specified. | ||
* | ||
* @param bamFile the path to the BAM file | ||
* @param granularity write the offset of every n-th alignment to the index | ||
* @throws IOException as per java IO contract | ||
*/ | ||
public static void createIndex(final Path bamFile, final long granularity) throws IOException { | ||
final Path splittingBaiFile = IOUtil.addExtension(bamFile, SBIIndex.FILE_EXTENSION); | ||
try (SeekableStream in = new SeekablePathStream(bamFile); OutputStream out = Files.newOutputStream(splittingBaiFile)) { | ||
createIndex(in, out, granularity); | ||
} | ||
} | ||
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/** | ||
* Perform indexing on the given BAM file, at the granularity level specified. | ||
* | ||
* @param in a seekable stream for reading the BAM file from | ||
* @param out the stream to write the index to | ||
* @param granularity write the offset of every n-th alignment to the index | ||
* @throws IOException as per java IO contract | ||
*/ | ||
public static void createIndex(final SeekableStream in, final OutputStream out, final long granularity) throws IOException { | ||
long recordStart = SAMUtils.findVirtualOffsetOfFirstRecordInBam(in); | ||
try (BlockCompressedInputStream blockIn = new BlockCompressedInputStream(in)) { | ||
blockIn.seek(recordStart); | ||
// Create a buffer for reading the BAM record lengths. BAM is little-endian. | ||
final ByteBuffer byteBuffer = ByteBuffer.allocate(4).order(ByteOrder.LITTLE_ENDIAN); | ||
final SBIIndexWriter indexWriter = new SBIIndexWriter(out, granularity); | ||
while (true) { | ||
try { | ||
recordStart = blockIn.getFilePointer(); | ||
// Read the length of the remainder of the BAM record (`block_size` in the SAM spec) | ||
InputStreamUtils.readFully(blockIn, byteBuffer.array(), 0, 4); | ||
final int blockSize = byteBuffer.getInt(0); | ||
// Process the record start position, then skip to the start of the next BAM record | ||
indexWriter.processRecord(recordStart); | ||
InputStreamUtils.skipFully(blockIn, blockSize); | ||
} catch (EOFException e) { | ||
break; | ||
} | ||
} | ||
indexWriter.finish(recordStart, in.length()); | ||
} | ||
} | ||
} |
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