Summary:
- Updating to PICRUSt2-v2.6.0 to work with new PICRUSt2-MPGA database. Please see Wiki page for full details.
- New PICRUSt2-MPGA database contains separate reference files and annotations for bacteria and archaea.
- New default workflow:
- Previous place sequences step is split to placing in bacterial and archaeal reference trees by default.
- Hidden state prediction is run separately for 16S for bacteria and archaea to determine NSTI for all sequences for both reference sets. The best-fitting domain for each sequence is then chosen (based on lowest NSTI) and the files are filtered to contain only those that fit the best.
- Hidden state prediction is then run for KOs and EC numbers for both bacteria and archaea.
- Combine bacterial and archaeal predictions for KOs and EC numbers.
- Closest reference genome is now given for all sequences.
- Added ete3 requirement.
- Changed several dependency versions, hoping this will fix issues for some Mac users
What's Changed
- PICRUSt2-v2.6.0 by @R-Wright-1 in #372
New Contributors
- @R-Wright-1 made their first contribution in #372
Full Changelog: v2.5.3...v2.6.0
Contributors
R-Wright-1