my_singcell

This is a Split-seq Scell Data analysis method. This pipeline can process the over 20000 cells in 3 days. http://science.sciencemag.org/content/360/6385/176.full

##Useage pipeline:

1. first step:

      files perpare: raw fastq files and barcode file
      change your paire-end fastq files name to R1.fastq(R1) and R2.fastq(R2)
   

2. then run:

    init.sh to initialize the directy and environment.