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Clarify infer strandedness step in subway map and text #1307

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Jun 18, 2024
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1 change: 1 addition & 0 deletions CHANGELOG.md
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Expand Up @@ -71,6 +71,7 @@ Thank you to everyone else that has contributed by reporting bugs, enhancements
- [PR #1297](https://github.com/nf-core/rnaseq/pull/1297) - Important! Template update for nf-core/tools v2.14.1
- [PR #1302](https://github.com/nf-core/rnaseq/pull/1302) - Add missing files from Tximport processing
- [PR #1304](https://github.com/nf-core/rnaseq/pull/1304) - Remove redundant gene TPM outputs
- [PR #1307](https://github.com/nf-core/rnaseq/pull/1307) - Clarify infer strandedness step in subway map and text
- [PR #1317](https://github.com/nf-core/rnaseq/pull/1317) - Strip problematic ifEmpty()
- [PR #1319](https://github.com/nf-core/rnaseq/pull/1319) - Reinstate oncomplete error messages
- [PR #1310](https://github.com/nf-core/rnaseq/pull/1310) - Reinstate pseudoalignment subworkflow config
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2 changes: 1 addition & 1 deletion README.md
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Expand Up @@ -23,7 +23,7 @@
![nf-core/rnaseq metro map](docs/images/nf-core-rnaseq_metro_map_grey.png)

1. Merge re-sequenced FastQ files ([`cat`](http://www.linfo.org/cat.html))
2. Sub-sample FastQ files and auto-infer strandedness ([`fq`](https://github.com/stjude-rust-labs/fq), [`Salmon`](https://combine-lab.github.io/salmon/))
2. Auto-infer strandedness by subsampling and pseudoalignment ([`fq`](https://github.com/stjude-rust-labs/fq), [`Salmon`](https://combine-lab.github.io/salmon/))
3. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
4. UMI extraction ([`UMI-tools`](https://github.com/CGATOxford/UMI-tools))
5. Adapter and quality trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/))
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66 changes: 19 additions & 47 deletions docs/images/nf-core-rnaseq_metro_map_grey.svg
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