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Update assets (#51)
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* update GATK bundle assets

* Update assets/genomes/generate_yaml_asset.sh

Co-authored-by: Louis LE NEZET <58640615+LouisLeNezet@users.noreply.github.com>

* move files

* keep existing assets

* add fasta_fai

* fix fasta

* fix files

* add fasta_dict

* add fasta_fai

* update CHANGELOG

---------

Co-authored-by: Louis LE NEZET <58640615+LouisLeNezet@users.noreply.github.com>
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maxulysse and LouisLeNezet authored Dec 4, 2024
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2 changes: 2 additions & 0 deletions .gitignore
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tmp/
.nf-test
.vscode
assets/all_*.txt
assets/ngi-igenomes_file_manifest.txt
2 changes: 2 additions & 0 deletions CHANGELOG.md
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Expand Up @@ -24,6 +24,7 @@ Initial release of nf-core/references, created with the [nf-core](https://nf-co.
- [42](https://github.com/nf-core/references/pull/42) - Add multiple references build tests
- [43](https://github.com/nf-core/references/pull/43) - Add support for multiple known_indels_vcf via s3 globs
- [47](https://github.com/nf-core/references/pull/47) - Add fasta assets for files in igenomes
- [51](https://github.com/nf-core/references/pull/51) - Add fasta_fai assets for files in igenomes

### Changed

Expand Down Expand Up @@ -51,6 +52,7 @@ Initial release of nf-core/references, created with the [nf-core](https://nf-co.
- [36](https://github.com/nf-core/references/pull/36) - Deal intervals generation
- [39](https://github.com/nf-core/references/pull/39) - Fix gtf generation and dependencies
- [50](https://github.com/nf-core/references/pull/50) - Minimal JAVA is 17
- [51](https://github.com/nf-core/references/pull/51) - Fix missing fasta assets for GATK build

### Dependencies

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59 changes: 59 additions & 0 deletions assets/generate_yaml_asset.sh
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#!/usr/bin/env bash

# Download or regenerate manifest file

# cf https://github.com/ewels/AWS-iGenomes/pull/22

# wget https://raw.githubusercontent.com/ewels/AWS-iGenomes/refs/heads/master/ngi-igenomes_file_manifest.txt

# aws s3 --no-sign-request ls --recursive s3://ngi-igenomes/igenomes/ | cut -d "/" -f 2- > tmp
# for i in `cat tmp`; do
# echo s3://ngi-igenomes/igenomes/$i >> manifest
# done
# mv manifest ngi-igenomes_file_manifest.txt
# rm tmp

# Remove existing assets
rm -rf igenomes/

# Generate base info in species/genome/build.yml

# All source fasta.fai
# ALL fais are coming from a fasta file of the same name
# Hence I use it to generate fasta + fai (and catch with that the fasta that are not following the gemome.fa name scheme)

cat ngi-igenomes_file_manifest.txt | grep "\.fai" | grep -v "Bowtie2Index" | grep -v "fai\.gz" > all_fai.txt

for i in `cat all_fai.txt`;
do
species=$(echo $i | cut -d "/" -f 5)
genome=$(echo $i | cut -d "/" -f 6)
build=$(echo $i | cut -d "/" -f 7)

mkdir -p igenomes/${species}/${genome}
if [ -f "igenomes/${species}/${genome}/${build}.yml" ]; then
build+="_2_"
fi
echo "- genome: '${build}'" > igenomes/${species}/${genome}/${build}.yml
echo " fasta: '${i::-4}'" >> igenomes/${species}/${genome}/${build}.yml
echo " source: '${genome}'" >> igenomes/${species}/${genome}/${build}.yml
echo " species: '${species}'" >> igenomes/${species}/${genome}/${build}.yml
echo " fasta_fai: '${i}'" >> igenomes/${species}/${genome}/${build}.yml
done

# # All source fasta.dict
# cat ngi-igenomes_file_manifest.txt | grep "\.dict" | grep -v "dict\.gz" | grep -v "dict\.old" > all_dict.txt

# # Generate base info in species/genome/build.yml

# for i in `cat all_dict.txt`;
# do
# species=$(echo $i | cut -d "/" -f 5)
# genome=$(echo $i | cut -d "/" -f 6)
# build=$(echo $i | cut -d "/" -f 7)

# echo " fasta_dict: '${i}'" >> igenomes/${species}/${genome}/${build}.yml
# done

# Homo_sapiens/GATK/GRCh37.yml should actually be Homo_sapiens/GATK/GRCh37decoy.yml
mv igenomes/Homo_sapiens/GATK/GRCh37.yml igenomes/Homo_sapiens/GATK/GRCh37decoy.yml
4 changes: 0 additions & 4 deletions assets/genomes/Homo_sapiens/GATK/GRCh37.yml

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8 changes: 0 additions & 8 deletions assets/genomes/Homo_sapiens/ucsc/hg19.yml

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8 changes: 0 additions & 8 deletions assets/genomes/Homo_sapiens/ucsc/hg38.yml

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- genome: GRCm39
- genome: mm39
fasta: https://hgdownload.soe.ucsc.edu/goldenPath/mm39/bigZips/mm39.fa.gz
gtf: https://hgdownload.soe.ucsc.edu/goldenPath/mm39/bigZips/genes/mm39.ncbiRefSeq.gtf.gz
mito_name: MT
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53 changes: 0 additions & 53 deletions assets/genomes/Saccharomyces_cerevisiae/Ensembl/R64-1-1.yml

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31 changes: 0 additions & 31 deletions assets/genomes/generate_yaml_asset.sh

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fasta: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Arabidopsis_thaliana"
fasta_fai: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/Ensembl/TAIR9/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Arabidopsis_thaliana"
fasta_fai: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/Ensembl/TAIR9/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/NCBI/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Arabidopsis_thaliana"
fasta_fai: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/NCBI/TAIR10/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/NCBI/build9.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Arabidopsis_thaliana"
fasta_fai: "s3://ngi-igenomes/igenomes/Arabidopsis_thaliana/NCBI/build9.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bacillus_cereus_ATCC_10987/NCBI/2004-02-13/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Bacillus_cereus_ATCC_10987"
fasta_fai: "s3://ngi-igenomes/igenomes/Bacillus_cereus_ATCC_10987/NCBI/2004-02-13/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Bacillus_subtilis_168"
fasta_fai: "s3://ngi-igenomes/igenomes/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/Ensembl/Btau_4.0/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/Ensembl/Btau_4.0/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/Btau_4.2/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/Btau_4.2/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/Btau_4.6.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/Btau_4.6.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/UMD_3.1.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/UMD_3.1.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/UMD_3.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/NCBI/UMD_3.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau4/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau4/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau6/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau6/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau7/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau7/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Bos_taurus"
fasta_fai: "s3://ngi-igenomes/igenomes/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WBcel215/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WBcel215/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WS210/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WS210/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WS220/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/Ensembl/WS220/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/NCBI/WS190/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/NCBI/WS190/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/NCBI/WS195/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/NCBI/WS195/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/UCSC/ce6/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Caenorhabditis_elegans"
fasta_fai: "s3://ngi-igenomes/igenomes/Caenorhabditis_elegans/UCSC/ce6/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Canis_familiaris/Ensembl/BROADD2/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Canis_familiaris"
fasta_fai: "s3://ngi-igenomes/igenomes/Canis_familiaris/Ensembl/BROADD2/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Canis_familiaris"
fasta_fai: "s3://ngi-igenomes/igenomes/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Canis_familiaris/NCBI/build2.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Canis_familiaris"
fasta_fai: "s3://ngi-igenomes/igenomes/Canis_familiaris/NCBI/build2.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Canis_familiaris/NCBI/build3.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Canis_familiaris"
fasta_fai: "s3://ngi-igenomes/igenomes/Canis_familiaris/NCBI/build3.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Canis_familiaris/UCSC/canFam2/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Canis_familiaris"
fasta_fai: "s3://ngi-igenomes/igenomes/Canis_familiaris/UCSC/canFam2/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Canis_familiaris"
fasta_fai: "s3://ngi-igenomes/igenomes/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Danio_rerio"
fasta_fai: "s3://ngi-igenomes/igenomes/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Danio_rerio/Ensembl/Zv9/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Danio_rerio"
fasta_fai: "s3://ngi-igenomes/igenomes/Danio_rerio/Ensembl/Zv9/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Danio_rerio/NCBI/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Danio_rerio"
fasta_fai: "s3://ngi-igenomes/igenomes/Danio_rerio/NCBI/GRCz10/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Danio_rerio/NCBI/Zv9/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Danio_rerio"
fasta_fai: "s3://ngi-igenomes/igenomes/Danio_rerio/NCBI/Zv9/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Danio_rerio"
fasta_fai: "s3://ngi-igenomes/igenomes/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Danio_rerio/UCSC/danRer7/Sequence/WholeGenomeFasta/genome.fa"
source: "UCSC"
species: "Danio_rerio"
fasta_fai: "s3://ngi-igenomes/igenomes/Danio_rerio/UCSC/danRer7/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/Ensembl/BDGP5.25/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Drosophila_melanogaster"
fasta_fai: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/Ensembl/BDGP5.25/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/Ensembl/BDGP5/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Drosophila_melanogaster"
fasta_fai: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/Ensembl/BDGP5/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
source: "Ensembl"
species: "Drosophila_melanogaster"
fasta_fai: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa.fai"
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fasta: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/NCBI/build4.1/Sequence/WholeGenomeFasta/genome.fa"
source: "NCBI"
species: "Drosophila_melanogaster"
fasta_fai: "s3://ngi-igenomes/igenomes/Drosophila_melanogaster/NCBI/build4.1/Sequence/WholeGenomeFasta/genome.fa.fai"
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