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Fix hamronization fargene input #411

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merged 8 commits into from
Aug 20, 2024
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jasmezz
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@jasmezz jasmezz commented Aug 12, 2024

This optimizes which hits from fARGene are summarized by hAMRonization: Before this PR, 2 files were in the hmmsearch output channel, but only one is required (the one starting with retrieved-genes-, as can be seen in the fARGene documentation:

image

Now, after the fargene module update, the ARG subworkflow pics the correct fargene output channel as input for hAMRonization and only the corrects show up in the final summary file (results/reports/hamronization_summarize/hamronization_combined_report.tsv tested and verified locally).

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
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  • If necessary, also make a PR on the nf-core/funcscan branch on the nf-core/test-datasets repository.
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github-actions bot commented Aug 12, 2024

nf-core lint overall result: Passed ✅ ⚠️

Posted for pipeline commit c820d20

+| ✅ 333 tests passed       |+
#| ❔   1 tests were ignored |#
!| ❗   1 tests had warnings |!

❗ Test warnings:

❔ Tests ignored:

✅ Tests passed:

  • files_exist - File found: .gitattributes
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  • files_exist - File found: CHANGELOG.md
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  • files_exist - File found: LICENSE or LICENSE.md or LICENCE or LICENCE.md
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  • nextflow_config - Config variable found: manifest.name
  • nextflow_config - Config variable found: manifest.nextflowVersion
  • nextflow_config - Config variable found: manifest.description
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  • nextflow_config - Config variable found: manifest.homePage
  • nextflow_config - Config variable found: timeline.enabled
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  • nextflow_config - Config variable found: process.cpus
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  • nextflow_config - Config variable found: params.outdir
  • nextflow_config - Config variable found: params.input
  • nextflow_config - Config variable found: params.validationShowHiddenParams
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  • nextflow_config - Config variable found: manifest.mainScript
  • nextflow_config - Config variable found: timeline.file
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  • nextflow_config - Config variable (correctly) not found: params.nf_required_version
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  • nextflow_config - Config variable (correctly) not found: params.igenomesIgnore
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  • nextflow_config - Config timeline.enabled had correct value: true
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  • nextflow_config - Config trace.enabled had correct value: true
  • nextflow_config - Config dag.enabled had correct value: true
  • nextflow_config - Config manifest.name began with nf-core/
  • nextflow_config - Config variable manifest.homePage began with https://github.com/nf-core/
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  • nextflow_config - Config params.custom_config_version is set to master
  • nextflow_config - Config params.custom_config_base is set to https://raw.githubusercontent.com/nf-core/configs/master
  • nextflow_config - Lines for loading custom profiles found
  • nextflow_config - nextflow.config contains configuration profile test
  • nextflow_config - Config default value correct: params.taxa_classification_tool= mmseqs2
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_db_id= Kalamari
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_searchtype= 2
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_lcaranks= kingdom,phylum,class,order,family,genus,species
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_taxlineage= 1
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_sensitivity= 5.0
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_orffilters= 2.0
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_lcamode= 3
  • nextflow_config - Config default value correct: params.taxa_classification_mmseqs_taxonomy_votemode= 1
  • nextflow_config - Config default value correct: params.annotation_tool= pyrodigal
  • nextflow_config - Config default value correct: params.annotation_bakta_db_downloadtype= full
  • nextflow_config - Config default value correct: params.annotation_bakta_mincontiglen= 1
  • nextflow_config - Config default value correct: params.annotation_bakta_translationtable= 11
  • nextflow_config - Config default value correct: params.annotation_bakta_gram= ?
  • nextflow_config - Config default value correct: params.annotation_prokka_kingdom= Bacteria
  • nextflow_config - Config default value correct: params.annotation_prokka_gcode= 11
  • nextflow_config - Config default value correct: params.annotation_prokka_mincontiglen= 1
  • nextflow_config - Config default value correct: params.annotation_prokka_evalue= 1e-06
  • nextflow_config - Config default value correct: params.annotation_prokka_coverage= 80
  • nextflow_config - Config default value correct: params.annotation_prokka_compliant= true
  • nextflow_config - Config default value correct: params.annotation_prodigal_transtable= 11
  • nextflow_config - Config default value correct: params.annotation_pyrodigal_transtable= 11
  • nextflow_config - Config default value correct: params.amp_ampir_model= precursor
  • nextflow_config - Config default value correct: params.amp_ampir_minlength= 10
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_cutoff= 0.6
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_aalength= 100
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_dbevalue= 5.0
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_hmmevalue= 0.06
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_windowstopcodon= 60
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_windowtransport= 11
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_ampir= .ampir.tsv
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_amplify= .amplify.tsv
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_macrel= .macrel.prediction
  • nextflow_config - Config default value correct: params.amp_ampcombi_parsetables_hmmsearch= .hmmer_hmmsearch.txt
  • nextflow_config - Config default value correct: params.amp_ampcombi_cluster_covmode= 0.0
  • nextflow_config - Config default value correct: params.amp_ampcombi_cluster_sensitivity= 4.0
  • nextflow_config - Config default value correct: params.amp_ampcombi_cluster_minmembers= 0
  • nextflow_config - Config default value correct: params.amp_ampcombi_cluster_mode= 1.0
  • nextflow_config - Config default value correct: params.amp_ampcombi_cluster_coverage= 0.8
  • nextflow_config - Config default value correct: params.amp_ampcombi_cluster_seqid= 0.4
  • nextflow_config - Config default value correct: params.arg_amrfinderplus_identmin= -1.0
  • nextflow_config - Config default value correct: params.arg_amrfinderplus_coveragemin= 0.5
  • nextflow_config - Config default value correct: params.arg_amrfinderplus_translationtable= 11
  • nextflow_config - Config default value correct: params.arg_deeparg_db_version= 2
  • nextflow_config - Config default value correct: params.arg_deeparg_model= LS
  • nextflow_config - Config default value correct: params.arg_deeparg_minprob= 0.8
  • nextflow_config - Config default value correct: params.arg_deeparg_alignmentevalue= 1e-10
  • nextflow_config - Config default value correct: params.arg_deeparg_alignmentidentity= 50
  • nextflow_config - Config default value correct: params.arg_deeparg_alignmentoverlap= 0.8
  • nextflow_config - Config default value correct: params.arg_deeparg_numalignmentsperentry= 1000
  • nextflow_config - Config default value correct: params.arg_fargene_hmmmodel= class_a,class_b_1_2,class_b_3,class_c,class_d_1,class_d_2,qnr,tet_efflux,tet_rpg,tet_enzyme
  • nextflow_config - Config default value correct: params.arg_fargene_minorflength= 90
  • nextflow_config - Config default value correct: params.arg_fargene_translationformat= pearson
  • nextflow_config - Config default value correct: params.arg_rgi_alignmenttool= BLAST
  • nextflow_config - Config default value correct: params.arg_rgi_data= NA
  • nextflow_config - Config default value correct: params.arg_rgi_split_prodigal_jobs= true
  • nextflow_config - Config default value correct: params.arg_abricate_db_id= ncbi
  • nextflow_config - Config default value correct: params.arg_abricate_minid= 80
  • nextflow_config - Config default value correct: params.arg_abricate_mincov= 80
  • nextflow_config - Config default value correct: params.arg_hamronization_summarizeformat= tsv
  • nextflow_config - Config default value correct: params.bgc_mincontiglength= 3000
  • nextflow_config - Config default value correct: params.bgc_antismash_contigminlength= 3000
  • nextflow_config - Config default value correct: params.bgc_antismash_hmmdetectionstrictness= relaxed
  • nextflow_config - Config default value correct: params.bgc_antismash_pfam2go= false
  • nextflow_config - Config default value correct: params.bgc_antismash_rre= false
  • nextflow_config - Config default value correct: params.bgc_antismash_taxon= bacteria
  • nextflow_config - Config default value correct: params.bgc_antismash_tfbs= false
  • nextflow_config - Config default value correct: params.bgc_deepbgc_score= 0.5
  • nextflow_config - Config default value correct: params.bgc_deepbgc_mergemaxproteingap= 0
  • nextflow_config - Config default value correct: params.bgc_deepbgc_mergemaxnuclgap= 0
  • nextflow_config - Config default value correct: params.bgc_deepbgc_minnucl= 1
  • nextflow_config - Config default value correct: params.bgc_deepbgc_minproteins= 1
  • nextflow_config - Config default value correct: params.bgc_deepbgc_mindomains= 1
  • nextflow_config - Config default value correct: params.bgc_deepbgc_minbiodomains= 0
  • nextflow_config - Config default value correct: params.bgc_deepbgc_classifierscore= 0.5
  • nextflow_config - Config default value correct: params.bgc_gecco_cds= 3
  • nextflow_config - Config default value correct: params.bgc_gecco_pfilter= 1e-09
  • nextflow_config - Config default value correct: params.bgc_gecco_threshold= 0.8
  • nextflow_config - Config default value correct: params.bgc_gecco_edgedistance= 0
  • nextflow_config - Config default value correct: params.custom_config_version= master
  • nextflow_config - Config default value correct: params.custom_config_base= https://raw.githubusercontent.com/nf-core/configs/master
  • nextflow_config - Config default value correct: params.max_cpus= 16
  • nextflow_config - Config default value correct: params.max_memory= 128.GB
  • nextflow_config - Config default value correct: params.max_time= 240.h
  • nextflow_config - Config default value correct: params.publish_dir_mode= copy
  • nextflow_config - Config default value correct: params.max_multiqc_email_size= 25.MB
  • nextflow_config - Config default value correct: params.validate_params= true
  • nextflow_config - Config default value correct: params.pipelines_testdata_base_path= https://raw.githubusercontent.com/nf-core/test-datasets/
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  • readme - README Nextflow minimum version badge matched config. Badge: 23.04.0, Config: 23.04.0
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  • pipeline_todos - No TODO strings found
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  • template_strings - Did not find any Jinja template strings (383 files)
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  • multiqc_config - assets/multiqc_config.yml found and not ignored.
  • multiqc_config - assets/multiqc_config.yml contains report_section_order
  • multiqc_config - assets/multiqc_config.yml contains export_plots
  • multiqc_config - assets/multiqc_config.yml contains report_comment
  • multiqc_config - assets/multiqc_config.yml follows the ordering scheme of the minimally required plugins.
  • multiqc_config - assets/multiqc_config.yml contains a matching 'report_comment'.
  • multiqc_config - assets/multiqc_config.yml contains 'export_plots: true'.
  • modules_structure - modules directory structure is correct 'modules/nf-core/TOOL/SUBTOOL'
  • base_config - conf/base.config found and not ignored.
  • base_config - GUNZIP found in conf/base.config and Nextflow scripts.
  • base_config - UNTAR found in conf/base.config and Nextflow scripts.
  • base_config - PROKKA found in conf/base.config and Nextflow scripts.
  • base_config - PRODIGAL_GBK found in conf/base.config and Nextflow scripts.
  • base_config - BAKTA_BAKTA found in conf/base.config and Nextflow scripts.
  • base_config - ABRICATE_RUN found in conf/base.config and Nextflow scripts.
  • base_config - AMRFINDERPLUS_RUN found in conf/base.config and Nextflow scripts.
  • base_config - DEEPARG_DOWNLOADDATA found in conf/base.config and Nextflow scripts.
  • base_config - DEEPARG_PREDICT found in conf/base.config and Nextflow scripts.
  • base_config - FARGENE found in conf/base.config and Nextflow scripts.
  • base_config - RGI_MAIN found in conf/base.config and Nextflow scripts.
  • base_config - AMPIR found in conf/base.config and Nextflow scripts.
  • base_config - AMPLIFY_PREDICT found in conf/base.config and Nextflow scripts.
  • base_config - AMP_HMMER_HMMSEARCH found in conf/base.config and Nextflow scripts.
  • base_config - MACREL_CONTIGS found in conf/base.config and Nextflow scripts.
  • base_config - BGC_HMMER_HMMSEARCH found in conf/base.config and Nextflow scripts.
  • base_config - ANTISMASH_ANTISMASHLITE found in conf/base.config and Nextflow scripts.
  • base_config - ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES found in conf/base.config and Nextflow scripts.
  • base_config - DEEPBGC_DOWNLOAD found in conf/base.config and Nextflow scripts.
  • base_config - DEEPBGC_PIPELINE found in conf/base.config and Nextflow scripts.
  • base_config - GECCO_RUN found in conf/base.config and Nextflow scripts.
  • base_config - HAMRONIZATION_ABRICATE found in conf/base.config and Nextflow scripts.
  • base_config - HAMRONIZATION_AMRFINDERPLUS found in conf/base.config and Nextflow scripts.
  • base_config - HAMRONIZATION_DEEPARG found in conf/base.config and Nextflow scripts.
  • base_config - HAMRONIZATION_RGI found in conf/base.config and Nextflow scripts.
  • base_config - HAMRONIZATION_FARGENE found in conf/base.config and Nextflow scripts.
  • base_config - HAMRONIZATION_SUMMARIZE found in conf/base.config and Nextflow scripts.
  • base_config - ARGNORM_DEEPARG found in conf/base.config and Nextflow scripts.
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  • base_config - ARGNORM_AMRFINDERPLUS found in conf/base.config and Nextflow scripts.
  • base_config - AMPCOMBI2_PARSETABLES found in conf/base.config and Nextflow scripts.
  • base_config - AMPCOMBI2_CLUSTER found in conf/base.config and Nextflow scripts.
  • modules_config - conf/modules.config found and not ignored.
  • modules_config - MULTIQC found in conf/modules.config and Nextflow scripts.
  • modules_config - GUNZIP found in conf/modules.config and Nextflow scripts.
  • modules_config - MMSEQS_DATABASES found in conf/modules.config and Nextflow scripts.
  • modules_config - MMSEQS_CREATEDB found in conf/modules.config and Nextflow scripts.
  • modules_config - MMSEQS_TAXONOMY found in conf/modules.config and Nextflow scripts.
  • modules_config - MMSEQS_CREATETSV found in conf/modules.config and Nextflow scripts.
  • modules_config - SEQKIT_SEQ found in conf/modules.config and Nextflow scripts.
  • modules_config - PROKKA found in conf/modules.config and Nextflow scripts.
  • modules_config - BAKTA_BAKTADBDOWNLOAD found in conf/modules.config and Nextflow scripts.
  • modules_config - BAKTA_BAKTA found in conf/modules.config and Nextflow scripts.
  • modules_config - PRODIGAL found in conf/modules.config and Nextflow scripts.
  • modules_config - PYRODIGAL found in conf/modules.config and Nextflow scripts.
  • modules_config - ABRICATE_RUN found in conf/modules.config and Nextflow scripts.
  • modules_config - AMRFINDERPLUS_UPDATE found in conf/modules.config and Nextflow scripts.
  • modules_config - AMRFINDERPLUS_RUN found in conf/modules.config and Nextflow scripts.
  • modules_config - DEEPARG_DOWNLOADDATA found in conf/modules.config and Nextflow scripts.
  • modules_config - DEEPARG_PREDICT found in conf/modules.config and Nextflow scripts.
  • modules_config - FARGENE found in conf/modules.config and Nextflow scripts.
  • modules_config - UNTAR_CARD found in conf/modules.config and Nextflow scripts.
  • modules_config - RGI_CARDANNOTATION found in conf/modules.config and Nextflow scripts.
  • modules_config - RGI_MAIN found in conf/modules.config and Nextflow scripts.
  • modules_config - AMPIR found in conf/modules.config and Nextflow scripts.
  • modules_config - AMPLIFY_PREDICT found in conf/modules.config and Nextflow scripts.
  • modules_config - AMP_HMMER_HMMSEARCH found in conf/modules.config and Nextflow scripts.
  • modules_config - MACREL_CONTIGS found in conf/modules.config and Nextflow scripts.
  • modules_config - BGC_HMMER_HMMSEARCH found in conf/modules.config and Nextflow scripts.
  • modules_config - ANTISMASH_ANTISMASHLITE found in conf/modules.config and Nextflow scripts.
  • modules_config - ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES found in conf/modules.config and Nextflow scripts.
  • modules_config - DEEPBGC_DOWNLOAD found in conf/modules.config and Nextflow scripts.
  • modules_config - DEEPBGC_PIPELINE found in conf/modules.config and Nextflow scripts.
  • modules_config - GECCO_RUN found in conf/modules.config and Nextflow scripts.
  • modules_config - HAMRONIZATION_ABRICATE found in conf/modules.config and Nextflow scripts.
  • modules_config - HAMRONIZATION_AMRFINDERPLUS found in conf/modules.config and Nextflow scripts.
  • modules_config - HAMRONIZATION_DEEPARG found in conf/modules.config and Nextflow scripts.
  • modules_config - HAMRONIZATION_RGI found in conf/modules.config and Nextflow scripts.
  • modules_config - HAMRONIZATION_FARGENE found in conf/modules.config and Nextflow scripts.
  • modules_config - HAMRONIZATION_SUMMARIZE found in conf/modules.config and Nextflow scripts.
  • modules_config - MERGE_TAXONOMY_HAMRONIZATION found in conf/modules.config and Nextflow scripts.
  • modules_config - ARG_TABIX_BGZIP found in conf/modules.config and Nextflow scripts.
  • modules_config - AMPCOMBI2_PARSETABLES found in conf/modules.config and Nextflow scripts.
  • modules_config - AMPCOMBI2_COMPLETE found in conf/modules.config and Nextflow scripts.
  • modules_config - AMPCOMBI2_CLUSTER found in conf/modules.config and Nextflow scripts.
  • modules_config - MERGE_TAXONOMY_AMPCOMBI found in conf/modules.config and Nextflow scripts.
  • modules_config - AMP_TABIX_BGZIP found in conf/modules.config and Nextflow scripts.
  • modules_config - COMBGC found in conf/modules.config and Nextflow scripts.
  • modules_config - ARGNORM_ABRICATE found in conf/modules.config and Nextflow scripts.
  • modules_config - ARGNORM_AMRFINDERPLUS found in conf/modules.config and Nextflow scripts.
  • modules_config - ARGNORM_DEEPARG found in conf/modules.config and Nextflow scripts.
  • modules_config - MERGE_TAXONOMY_COMBGC found in conf/modules.config and Nextflow scripts.
  • modules_config - BGC_TABIX_BGZIP found in conf/modules.config and Nextflow scripts.
  • modules_config - DRAMP_DOWNLOAD found in conf/modules.config and Nextflow scripts.
  • nfcore_yml - Repository type in .nf-core.yml is valid: pipeline
  • nfcore_yml - nf-core version in .nf-core.yml is set to the latest version: 2.14.1

Run details

  • nf-core/tools version 2.14.1
  • Run at 2024-08-15 12:11:58

@jfy133 jfy133 marked this pull request as ready for review August 13, 2024 09:32
@jasmezz jasmezz merged commit 89d5b82 into dev Aug 20, 2024
@jasmezz jasmezz deleted the fix-hamronization-fargene-input branch August 20, 2024 07:33
@jasmezz jasmezz linked an issue Aug 21, 2024 that may be closed by this pull request
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Optimize antismash usage docs
3 participants