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We currently have a single trimming parameter for all libraries. This can become an issue if submitting half-UDG and non-UDG libraries together via TSV. The former need 2-3bp trimmed, but the latter can need 7-10 bp. So either you lose a bunch of data for no reason, or you retain damage in your data.
We could branch the input by UDG treatment/SS/DS and have separate parameters for the end user to specify how much to trim in which libraries.
Something like this --bamutils_clip_left becomes --bamutils_clip_left_halfudg & --bamutils_clip_left_nonudg
Then the trimming process entails an if clause changing the specific parameters by library/udg type.
The text was updated successfully, but these errors were encountered:
We currently have a single trimming parameter for all libraries. This can become an issue if submitting half-UDG and non-UDG libraries together via TSV. The former need 2-3bp trimmed, but the latter can need 7-10 bp. So either you lose a bunch of data for no reason, or you retain damage in your data.
We could branch the input by UDG treatment/SS/DS and have separate parameters for the end user to specify how much to trim in which libraries.
Something like this
--bamutils_clip_left
becomes--bamutils_clip_left_halfudg
&--bamutils_clip_left_nonudg
Then the trimming process entails an if clause changing the specific parameters by library/udg type.
The text was updated successfully, but these errors were encountered: