understanding individual postanalysis results_Preprocess Table

[singhd6]

Hi I want to understand the preprocess table, my command to generate the table was:

individual_result.preprocessTable_DS.xlsx
According to my knowledge and according to mixcr documentation the explanation for each column is:

<style> </style>

characteristic:  name for a group of metrics for which the same downsampling was applied

preprocessor: the name of the overall preprocessing chain applied to the dataset
nElementsBefore: number of clonotypes before any preprocessing applied (that is in the initial dataset)
sumWeightBefore: sumWeightBefore
nElementsAfter: number of clonotypes in the dataset after all preprocessing applied
sumWeightBefore: total weight of all clonotypes before any preprocessing applied
nElementsAfter: number of clonotypes in the dataset after all preprocessing applied
preprocessor#i and following columns: report columns for i-th step in the preprocessing chain

 What does each column represent: <style> </style>

nElementsBefore

<style> </style>

sumWeightBefore

<style> </style>

nElementsAfter

<style> </style>

sumWeightAfter

<style> </style>

preprocessor#1

<style> </style>

nElementsBefore#1

<style> </style>

nElementsAfter#1

xmlns:x="urn:schemas-microsoft-com:office:excel"
xmlns="http://www.w3.org/TR/REC-html40">

<style> </style>

sumWeightBefore#1

sumWeightAfter#1
preprocessor#2
nElementsBefore#2
sumWeightBefore#2
nElementsAfter#2
sumWeightAfter#2
preprocessor#3

And then in the final frequency table how are we deciding the frequencies or fraction of v gene usage? Is it the number of total clones divided by the number of clones used by v gene?
I understand the explanation of gene frequencies but where did it come from?
frequencies for each v gene/segment present in the sample, during the creation of plot the weight or numbers represent the fraction of clones with a certain v gene regardless of their abundance in the repertoire.

[mizraelson]

Since you did not use any weight function this is a fraction of clones with a certain V gene. The number of clones with a certain V gene divided by the total number of clones.

The columns in the preprocess tables represent the number of clones (nElements) and the number of read (sumWeight) before and after each per-processor was applied.

[singhd6]

Thank you very much.
Sincerely,
Divya