Only minor changes: I fixed the program so that it should always recognise non-ASCII (accented) characters in sequence names rather than than this relying on the system default, and increased the maximum characters per line from 250 to 500. In addition, a new option to include gaps in the count of sequences when determining the threshold at each position, or not, was added (see revised docs).
The MacOSX version of the app should work on all Mac OS from High Sierra up. As the PyInstaller packages do not appear to be downwards compatible, I have included a version packaged under Sierra as well. Anyone still running even earlier MacOS versions, sorry, you'll have to run direct from python.