Skip to content

Commit

Permalink
Always use read alignment to estimate CDR3 abundance now. This would …
Browse files Browse the repository at this point in the history
…make the abundance estimation and read assignment consistent.
  • Loading branch information
mourisl committed Jun 28, 2024
1 parent 7b4fcf1 commit 7ef8d05
Showing 1 changed file with 3 additions and 2 deletions.
5 changes: 3 additions & 2 deletions run-trust4
Original file line number Diff line number Diff line change
Expand Up @@ -7,7 +7,7 @@ use Cwd qw(cwd abs_path) ;
use File::Basename ;
use File::Path qw(make_path) ;

my $version = "v1.1.2-r517" ;
my $version = "v1.1.2-r519" ;

die "TRUST4 $version usage: ./run-trust4 [OPTIONS]:\n".
"Required:\n".
Expand Down Expand Up @@ -487,8 +487,9 @@ if ( $stage <= 1 )
# Annotation
if ( $stage <= 2 )
{
if ( $hasBarcode == 0 || $hasUmi == 1 )
if (1) #$hasBarcode == 0 || $hasUmi == 1)
{
# Always use the read now. For consistent and reproducible abundance estimation if user want to examine read assignment.
$annotatorArgs .= " -r ${prefix}_assembled_reads.fa" ;
}
else
Expand Down

0 comments on commit 7ef8d05

Please sign in to comment.