f5c-v1.1
-
the experimental resquiggle module (contributed by @hiruna72) for signal to basecall alignment of DNA and RNA. Output format is documented here. Usage:
Usage: f5c [OPTIONS] reads.fastq signals.blow5 options: -t INT number of processing threads [8] -K INT batch size (max number of reads loaded at once) [512] -B FLOAT[K/M/G] max number of bases loaded at once [5.0M] -h help -o FILE output to file [stdout] -x STR parameter profile to be used for better performance (always applied before other options) e.g., laptop, desktop, hpc; see https://f5c.page.link/profiles for the full list -c print in paf format --verbose INT verbosity level [0] --version print version --kmer-model FILE custom nucleotide k-mer model file (format similar to test/r9-models/r9.4_450bps.nucleotide.6mer.template.model) --rna the dataset is direct RNA
-
f5c can be now built without HDF5 support (
./configure --disable-hdf5 && make
) for anyone who wants to easily compile and work only with S/BLOW5 files -
new option
--skip-slow-idx
added to f5c index:, which disable f5c from building the .idx for the slow5 file (useful when a slow5 index is already available) -
error messages improved for slow5 index