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# Sphinx build info version 1 | ||
# This file hashes the configuration used when building these files. When it is not found, a full rebuild will be done. | ||
config: 91a91fe9e4d6247399847d5e62aa629b | ||
config: cf47e53cc60f3464c18286f20c0ab856 | ||
tags: 645f666f9bcd5a90fca523b33c5a78b7 |
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.. _example_visium: | ||
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Visium | ||
====== | ||
====== | ||
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Follow this step by step guide to process and visualise a Visium sample in your web browser. | ||
It can be used on any POSIX compatible system (Linux, OS X, etc). | ||
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**#1. Check Git is installed** | ||
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Make sure git 2.17 or later is installed on your computer by using the command: | ||
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.. code-block:: shell | ||
:caption: Input | ||
git --version | ||
.. code-block:: shell | ||
:caption: Output | ||
git version 2.25.1 | ||
**#2. Clone the WebAtlas pipeline repository** | ||
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Make a copy of the WebAtlas repo and change directory into the new repo: | ||
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.. code-block:: shell | ||
:caption: Input | ||
git clone git@github.com:haniffalab/webatlas-pipeline.git | ||
cd webatlas-pipeline | ||
**#3. Check java is installed** | ||
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Make sure java 11 later is installed on your computer by using the command: | ||
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.. code-block:: shell | ||
:caption: Input | ||
java -version | ||
openjdk version "11" | ||
**#4. Install Nextflow** | ||
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Enter this command in your terminal to install nextflow in the current directory: | ||
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.. code-block:: shell | ||
:caption: Input | ||
curl -s https://get.nextflow.io | bash | ||
**#5. Check Docker is installed** | ||
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Make sure Docker Engine 20.10 later is installed on your computer by using the command: | ||
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.. code-block:: shell | ||
:caption: Input | ||
docker version | ||
.. code-block:: shell | ||
:caption: Output | ||
Client: Docker Engine - Community | ||
**#6. Build the docker images** | ||
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.. code-block:: shell | ||
:caption: Input | ||
cd docker | ||
./build-docker-imgs.sh | ||
cd ../ | ||
.. code-block:: shell | ||
:caption: Output | ||
somet | ||
**#7. Download the data** | ||
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.. code-block:: shell | ||
:caption: Input | ||
wget https://www.something.com | ||
tar ... | ||
.. code-block:: shell | ||
:caption: Output | ||
dgdf | ||
**#8. Download the input configuration file** | ||
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.. code-block:: shell | ||
:caption: Input | ||
wget https://www.something.com | ||
tar ... | ||
.. code-block:: shell | ||
:caption: Output | ||
dgdf | ||
**#9. Run the pipeline** | ||
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.. code-block:: shell | ||
:caption: Input | ||
nextflow run main.nf -params-file visium.yaml -entry Full_pipeline | ||
.. code-block:: shell | ||
:caption: Output | ||
dgdf | ||
**#10. Check execution was successful** | ||
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**#11. Serve the data output through a web browser** | ||
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**#12. Load data in your browser** | ||
|
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.rst-content .code-block-caption { | ||
text-align: left; | ||
padding: 2px; | ||
} |
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