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v1.1.1
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galaxy001 committed Oct 7, 2014
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4 changes: 3 additions & 1 deletion INSTALL
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1. Dependencies
===============
pIRS requires the following libraries to compile:
pIRS requires the following libraries(with *-devel packages) to compile:

zlib (http://www.zlib.net/)
Boost Libraries (http://www.boost.org/)
glibc-static
libstdc++-static

pIRS requires the following tools to generate and analyse profiles:

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7 changes: 7 additions & 0 deletions NEWS
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version 1.11
Fix one bug which lead to terminate called after throwing an instance of 'std::out_of_range' while simulate indel-error on reads with "pirs simulate".

version 1.10
Fix one bug which will result in overlapping variation region in program "pirs diploid".
Add option -e to simulate different substiution-error rate in program "pirs simulate".

version 1.01
Add EAMSS filter from CASAVA v.1.8.0 .

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9 changes: 5 additions & 4 deletions README
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-o <string> output prefix (default=ref_sequence)
-h output help information and exit

3.2 pirs simualte
3.2 pirs simulate
Simulate sequencing data of Illumina sequencing platform

Usage: pirs simualte [OPTIONS]
Usage: pirs simulate [OPTIONS]
-i <string> input reference sequence (file.fa / file.fa.gz)
-I <string> another input reference for diploid genome (generated by "pirs diploid",
set only when simulating reads of diploid genome)
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e) simulate_seq.o, simulate_seq.e: records of the program running information.
4.2 Simulation result of Illumina sequencing data for haploid genome.
Example command line:
pirs simualte -i Human_ref.fa -m 170 -l 90 -x 5 -v 10 -o Human >simulate_170.o 2>simulate_170.e
pirs simulate -i Human_ref.fa -m 170 -l 90 -x 5 -v 10 -o Human >simulate_170.o 2>simulate_170.e
Output files:
a) Human_90_170_1.fq.gz, Human_90_170_2.fq.gz: the pair-end read files.
b) Human_90_170.error_rate.distr: the error distribution file.
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e) simulate_170.o, simulate_170.e: record the running information of program.
4.3 Simulation result of Illumina sequencing data for diploid genome.
Example command line:
pirs simualte -i Human_ref.fa -I Human.snp.indel.invertion.fa.gz -m 800 -l 70 -x 5 -v 10
pirs simulate -i Human_ref.fa -I Human.snp.indel.invertion.fa.gz -m 800 -l 70 -x 5 -v 10
-o Human >simulate_800.o 2>simulate_800.e
Output files:
a) Human_70_800_1.fq.gz, Human_70_800_2.fq.gz: the pair-end read files.
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of output data in file *.error_rate.distr .

For update & support, please refer to http://code.google.com/p/pirs/ .

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