Updates based on testing

sequence_processing_pipeline/Commands.py

@@ -1,7 +1,8 @@
 import glob
 import gzip
 import os
-from sequence_processing_pipeline.util import iter_paired_files
+from sequence_processing_pipeline.util import (iter_paired_files,
+                                               determine_orientation)
 
 
 def split_similar_size_bins(data_location_path, max_file_list_size_in_gb,
@@ -14,21 +15,17 @@ def split_similar_size_bins(data_location_path, max_file_list_size_in_gb,
     :return: The number of output-files created, size of largest bin.
     '''
 
-    # SPP root paths are of the form:
-    # ../72dc6080-c453-418e-8a47-1ccd6d646a30/ConvertJob, and contain only
-    # directories named after projects e.g:
-    # 72dc6080-c453-418e-8a47-1ccd6d646a30/ConvertJob/Tell_Seq_15196.
-    # Each of these directories contain R1 and R2 fastq files. Hence, path
-    # is now the following:
-    # add one more level to account for project_names nested under ConvertJob
-    # dir.
-    # this will ignore the _I1_ reads that appear in the integrated result.
-    fastq_paths = glob.glob(data_location_path + '*/*/*.fastq.gz')
-
-    # case-specific filter for TellSeq output directories that also contain
-    # _I1_ files. Ensure paths are still sorted afterwards.
-    fastq_paths = [x for x in fastq_paths if '_I1_001.fastq.gz' not in x]
-    fastq_paths = sorted(fastq_paths)
+    # to prevent issues w/filenames like the ones below from being mistaken
+    # for R1 or R2 files, use determine_orientation().
+    # LS_8_22_2014_R2_SRE_S2_L007_I1_001.fastq.gz
+    # LS_8_22_2014_R1_SRE_S3_L007_I1_001.fastq.gz
+
+    # since the names of all fastq files are being scanned for orientation,
+    # collect all of them instead of mistakenly pre-filtering some files.
+    # fastq_paths = glob.glob(data_location_path + '/*/*_R?_*.fastq.gz')
+    fastq_paths = glob.glob(data_location_path + '/*/*.fastq.gz')
+    fastq_paths = [x for x in fastq_paths
+                   if determine_orientation(x) in ['R1', 'R2']]
 
     # convert from GB and halve as we sum R1
     max_size = (int(max_file_list_size_in_gb) * (2 ** 30) / 2)
@@ -114,21 +111,43 @@ def demux(id_map, fp, out_d, task, maxtask):
             openfps[idx] = current_fp
 
     # setup a parser
-    id_ = iter(fp)
+    seq_id = iter(fp)
     seq = iter(fp)
     dumb = iter(fp)
     qual = iter(fp)
 
-    for i, s, d, q in zip(id_, seq, dumb, qual):
-        fname_encoded, id_ = i.split(delimiter, 1)
+    for i, s, d, q in zip(seq_id, seq, dumb, qual):
+        # '@1', 'LH00444:84:227CNHLT4:7:1101:41955:2443/1'
+        # '@1', 'LH00444:84:227CNHLT4:7:1101:41955:2443/1 BX:Z:TATGACACATGCGGCCCT' # noqa
+        # '@baz/1
+
+        fname_encoded, sid = i.split(delimiter, 1)
 
         if fname_encoded not in openfps:
             continue
 
-        orientation = id_[-2]  # -1 is \n
         current_fp = openfps[fname_encoded]
-        id_ = rec + id_
-        current_fp[orientation].write(id_)
+
+        # remove '\n' from sid and split on all whitespace.
+        tmp = sid.strip().split()
+
+        if len(tmp) == 1:
+            # sequence id line contains no optional metadata.
+            # don't change sid.
+            # -1 is \n
+            orientation = sid[-2]
+            sid = rec + sid
+        elif len(tmp) == 2:
+            sid = tmp[0]
+            metadata = tmp[1]
+            # no '\n'
+            orientation = sid[-1]
+            # hexdump confirms separator is ' ', not '\t'
+            sid = rec + sid + ' ' + metadata + '\n'
+        else:
+            raise ValueError(f"'{sid}' is not a recognized form")
+
+        current_fp[orientation].write(sid)
         current_fp[orientation].write(s)
         current_fp[orientation].write(d)
         current_fp[orientation].write(q)

sequence_processing_pipeline/FastQCJob.py

@@ -278,11 +278,13 @@ def _generate_job_script(self):
         lines.append(f"#SBATCH -n {self.nprocs}")
         lines.append("#SBATCH --time %d" % self.wall_time_limit)
         lines.append(f"#SBATCH --mem {self.jmem}")
+        lines.append('#SBATCH --constraint="intel"')
 
         lines.append("#SBATCH --array 1-%d%%%d" % (
             len(self.commands), self.pool_size))
 
         lines.append("set -x")
+        lines.append("set +e")
         lines.append('date')
         lines.append('hostname')
         lines.append('echo ${SLURM_JOBID} ${SLURM_ARRAY_TASK_ID}')

sequence_processing_pipeline/GenPrepFileJob.py

@@ -13,7 +13,7 @@
 class GenPrepFileJob(Job):
     def __init__(self, run_dir, convert_job_path, qc_job_path, output_path,
                  input_file_path, seqpro_path, modules_to_load,
-                 qiita_job_id, is_amplicon=False):
+                 qiita_job_id, reports_path, is_amplicon=False):
 
         super().__init__(run_dir,
                          output_path,
@@ -31,13 +31,22 @@ def __init__(self, run_dir, convert_job_path, qc_job_path, output_path,
         self.commands = []
         self.has_replicates = False
         self.replicate_count = 0
+        self.reports_path = reports_path
 
         # make the 'root' of your run_directory
         makedirs(join(self.output_path, self.run_id), exist_ok=True)
         # copy bcl-convert's Stats-equivalent directory to the
         # run_directory
-        copytree(join(convert_job_path, 'Reports'),
-                 join(self.output_path, self.run_id, 'Reports'))
+
+        # This directory will already exist on restarts, hence avoid
+        # copying.
+        reports_dir = join(self.output_path, self.run_id, 'Reports')
+
+        if exists(reports_dir):
+            self.is_restart = True
+        else:
+            self.is_restart = False
+            copytree(self.reports_path, reports_dir)
 
         # extracting from either convert_job_path or qc_job_path should
         # produce equal results.
@@ -52,22 +61,26 @@ def __init__(self, run_dir, convert_job_path, qc_job_path, output_path,
 
             dst = join(self.output_path, self.run_id, project)
 
-            if self.is_amplicon:
-                if exists(amplicon_seq_dir):
-                    makedirs(dst, exist_ok=True)
-                    symlink(amplicon_seq_dir, join(dst, 'amplicon'))
-            else:
-                if exists(filtered_seq_dir):
-                    makedirs(dst, exist_ok=True)
-                    symlink(filtered_seq_dir, join(dst, 'filtered_sequences'))
-
-                if exists(trimmed_seq_dir):
-                    makedirs(dst, exist_ok=True)
-                    symlink(trimmed_seq_dir, join(dst, 'trimmed_sequences'))
-
-                if exists(fastp_rept_dir):
-                    makedirs(dst, exist_ok=True)
-                    symlink(fastp_rept_dir, join(dst, 'json'))
+            if not self.is_restart:
+                # these will already be created if restarted.
+                if self.is_amplicon:
+                    if exists(amplicon_seq_dir):
+                        makedirs(dst, exist_ok=True)
+                        symlink(amplicon_seq_dir, join(dst, 'amplicon'))
+                else:
+                    if exists(filtered_seq_dir):
+                        makedirs(dst, exist_ok=True)
+                        symlink(filtered_seq_dir, join(dst,
+                                                       'filtered_sequences'))
+
+                    if exists(trimmed_seq_dir):
+                        makedirs(dst, exist_ok=True)
+                        symlink(trimmed_seq_dir, join(dst,
+                                                      'trimmed_sequences'))
+
+                    if exists(fastp_rept_dir):
+                        makedirs(dst, exist_ok=True)
+                        symlink(fastp_rept_dir, join(dst, 'json'))
 
         # seqpro usage:
         # seqpro path/to/run_dir path/to/sample/sheet /path/to/fresh/output_dir