geCAI

MCMC method for estimating geCAI codon weights from CDS and mRNA abundance data

Running geCAI

geCAI.pl -i <SEQUENCE_FILE> -e <EXPRESSION_FILE>

OPTIONS:
 -i <file>	FASTA format file containing multiple CDS sequences
 -e <file>	Tab-delimitted text file of abundance estimates for CDS
 -g <int>	Number of generations for MCMC [Default = 5000]
 -p <int>	Print frequency [Default = 100]
 -w <file>	Specify staring codon weights [Defualt = random]

EXAMPLE FILES

Package contains example sequence and mRNA files. To run geCAI on these files execute

geCAI.pl -i Example_Sequences.fasta -e Example_Expression_Data.txt

LICENSE:

Distributed under the GNU General Public License (GPLv3). See License.md

CITATION:

When publishing work that uses geCAI please cite: Nascimento & Kelly et al. (2018)