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Co-authored-by: Jessica Mattick <jmattick@pacificbiosciences.com>
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--- | ||
layout: default | ||
title: Examples | ||
nav_order: 4 | ||
description: "Skera examples" | ||
--- | ||
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## CLI example | ||
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Data generated using the [MAS-Seq for single-cell IsoSeq kit](https://www.pacb.com/products-and-services/applications/rna-sequencing/single-cell-rna-sequencing/) on either Sequel IIe or Revio can be downloaded [here](https://downloads.pacbcloud.com/public/dataset/MAS-Seq/). | ||
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``` | ||
# download HiFi reads for MAS-Seq PBMCs run on Sequel IIe | ||
wget https://downloads.pacbcloud.com/public/dataset/MAS-Seq/DATA-SQ2-PBMC_5kcells/0-CCS/m64476e_220618_014917.hifi_reads.bam | ||
# download MAS adapter fasta | ||
wget https://downloads.pacbcloud.com/public/dataset/MAS-Seq/REF-MAS_adapters/MAS-Seq_Adapter_v1/mas16_primers.fasta | ||
# run skera split to generate segmented reads | ||
skera split m64476e_220618_014917.hifi_reads.bam mas16_primers.fasta segmented.bam | ||
``` | ||
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An example of the `segmented.bam` output can be found [here](https://downloads.pacbcloud.com/public/dataset/MAS-Seq/DATA-SQ2-PBMC_5kcells/1-Sreads/) |
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--- | ||
layout: default | ||
title: Output Files | ||
nav_order: 4 | ||
description: "Skera Output Files" | ||
--- | ||
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## Output Files | ||
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### Segmented BAM files | ||
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* `segmented.bam` | ||
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_Skera_ outputs a BAM file containing all [segmented reads](/read-segments) that have sequential leading and trailing adapters based on the order of [adapters](/adapters) in the input fasta. | ||
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* `segmented.non_passing.bam` | ||
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The `non_passing.bam` file contains the bam records that have adapters found in non-sequential order. This file is necessary for `skera undo`. | ||
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### Reports | ||
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* `summary.csv` | ||
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The `summary.csv` contains general summary statistics for read segmentation. | ||
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* `ligations.csv` | ||
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The `ligations.csv` file contains the counts of all leading and trailing adapter combinations found in all passing and non-passing segments. | ||
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* `read_lengths.csv` | ||
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The `read_lengths.csv` file contains the parent HiFi and s-read lengths for all passing segments. |