Full length 16S amplicons from Chunlab collaboration

Instrument:  PacBio RS II
Chemistry:  C2
Enzyme: P4

Summary

This dataset includes several barcoded samples of full-length 16S amplicon sequences from three samples, plus a mock community. Results of analysis of this data are presented in the poster Analysis of Full-Length Metagenomic 16S Genes by SMRT® Sequencing (pdf). Experiments done in collaboration with Chunlab.

Related Information

Some scripts and suggestions for analysis of full-length 16S sequences are available on Devnet.

Sample metadata is as follows:

Water: Surface sea water sample from Oido, Siheung, Gyeonggi-do, South Korea.

Soil: Grassland soil from Seoul National University. Top soil (0-10cm), pH 4.88, water content 28.5%

Cecum: Mouse cecum.

Mock Community pGEM-T easy vector, 27f-1492r primer set

Clone No.	Coordindates	Site
EMC-0001	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0003	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0004	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0010	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0029	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0030	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0038	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0040	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0047	37° 21' N, 127° 0' E	Oil contaminated soil on Mt. Baekwoon
EMC-0061	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0063	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0068	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0077	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0081	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0093	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0097	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0109	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0124	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0125	36° 44' N, 126° 49' E	Rice paddy soil
EMC-0130	36° 44' N, 126° 49' E	Rice paddy soil

Download Dataset

• Bacterial 16S from a water sample (2.5 GB tar.gz)
• Bacterial 16S from a soil sample (3.1 GB tar.gz)
• Bacterial 16S from a cecum sample (3.1 GB tar.gz)
• Bacterial 16S from a mock community (3.2 GB tar.gz)