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This pipeline takes the raw metagenomic WGS reads as an input, assemble them, separate the contigs in MAGs, taxonomically classify the MAGs and quantify the ARGs in the MAGs. The pipeline is built using R Shiny and people can run end to end analysis by doing few clicks on the screen.

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MetaSutra

This is the code to run the app described in the manuscript:

Not Available Yet

The app is hosted on Shinyapps.io here:

Not Available Yet

To run this app locally on your machine, download R and run the following commands once to set up the environment:

source("server.R")
source("ui.R")
runApp()

You may now run the shiny app with just one command in R:

shiny::runGitHub("MetaSutra", "Nirmal2310") # Make Sure that the shiny package is installed in the R.

Nirmal Singh Mahar1, Anshul Budhraja2, Suman Pakala3, Ishaan Gupta1*, Seesandra V. Rajagopala3*.

1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi, India-110016

2Department of Medicine, Université de Montréal, Quebec, Canada

3Vanderbilt University Medical Center, Nashville, USA

*Corresponding Author

We would appreciate reports of any issues with the app via the issues option of GitHub or by emailing metasutra.help@gmail.com.

Instructions

Instructions can be found here: https://github.com/Nirmal2310/MetaSutra/blob/main/Tabs/additional_information.md

Licensing

This shiny code is licensed under the GPLv3. Please see the file LICENSE.txt for information.

MetaSutra App
Shiny App for analysis and visualization of Metagenomics Data.
Copyright (C) 2024 Nirmal Singh Mahar

This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, either version 3 of the License or
(at your option) any later version.

This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
GNU General Public License for more details.

You should have received a copy of the GNU General Public License
and this program.  If not, see <http://www.gnu.org/licenses/>.

You may contact the author of this code, Nirmal Singh Mahar, at <bez207518@iitd.ac.in>

Software adapted for use in the pipeline:

DOI

[DOI]

About

This pipeline takes the raw metagenomic WGS reads as an input, assemble them, separate the contigs in MAGs, taxonomically classify the MAGs and quantify the ARGs in the MAGs. The pipeline is built using R Shiny and people can run end to end analysis by doing few clicks on the screen.

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