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178 changes: 1 addition & 177 deletions 2403-canvas/home_schedule.html
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Johan Reimegård
</td>
</tr>
<tr grouplength="7">
<tr groupLength="7">
<td colspan="3" style="background:white;">
<strong>
<p style="font-size:110%;padding-top:10px;padding-bottom:5px;margin-top:10px;">
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Nima Rafati
</td>
</tr>
<tr grouplength="8">
<td colspan="3" style="background:white;">
<strong>
<p style="font-size:110%;padding-top:10px;padding-bottom:5px;margin-top:10px;">
<span style="font-weight:bold;"><span style="color:#95b540;"><i class="fas fa-calendar" style="margin-left:5px;margin-right:5px;"></i></span>14-Mar-2024</span><span style="color:white;border-radius:4px;padding-right:4px;padding-left:4px;background:#95b540;margin-left:9px;margin-right:7px;">Thu</span><span style="color:#95b540;"><i class="fas fa-map-marker-alt" style="margin-left:5px;margin-right:5px;"></i></span>Experimental classroom (10:203)
</p>
</strong>
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
09:00 - 10:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">DGE</span>
</td>
<td style="text-align:left;">
Julie Lorent
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
10:00 - 10:30
</td>
<td style="text-align:left;width: 16em; ">
<span style="color:white;font-weight:bold;border-radius:4px;padding:4px;background:#BDBDBD;">Coffee Break</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
10:30 - 12:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">DGE advanced</span>
</td>
<td style="text-align:left;">
Julie Lorent
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
12:00 - 13:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="color:white;font-weight:bold;border-radius:4px;padding:4px;background:#BDBDBD;">Lunch</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
13:00 - 14:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">GSA &amp; GSEA</span>
</td>
<td style="text-align:left;">
Nima Rafati
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
14:00 - 14:30
</td>
<td style="text-align:left;width: 16em; ">
<span style="color:white;font-weight:bold;border-radius:4px;padding:4px;background:#BDBDBD;">Coffee Break</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
14:30 - 15:30
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">GSA &amp; GSEA</span>
</td>
<td style="text-align:left;">
Nima Rafati
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
15:30 - 16:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">Online Repositories</span>
</td>
<td style="text-align:left;">
Johan Reimegård
</td>
</tr>
<tr groupLength="7">
<td colspan="3" style="background:white;">
<strong>
<p style="font-size:110%;padding-top:10px;padding-bottom:5px;margin-top:10px;">
<span style="font-weight:bold;"><span style="color:#95b540;"><i class="fas fa-calendar" style="margin-left:5px;margin-right:5px;"></i></span>15-Mar-2024</span><span style="color:white;border-radius:4px;padding-right:4px;padding-left:4px;background:#95b540;margin-left:9px;margin-right:7px;">Fri</span><span style="color:#95b540;"><i class="fas fa-map-marker-alt" style="margin-left:5px;margin-right:5px;"></i></span>Experimental classroom (10:203)
</p>
</strong>
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
09:00 - 10:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">Reading Bioinformatics</span>
</td>
<td style="text-align:left;">
Johan Reimegård
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
10:00 - 10:30
</td>
<td style="text-align:left;width: 16em; ">
<span style="color:white;font-weight:bold;border-radius:4px;padding:4px;background:#BDBDBD;">Coffee Break</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
10:30 - 12:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">Pseudoaligners</span>
</td>
<td style="text-align:left;">
Johan Reimegård
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
12:00 - 13:30
</td>
<td style="text-align:left;width: 16em; ">
<span style="color:white;font-weight:bold;border-radius:4px;padding:4px;background:#BDBDBD;">Lunch</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
13:30 - 14:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">Free Exercises</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
14:00 - 14:30
</td>
<td style="text-align:left;width: 16em; ">
<span style="color:white;font-weight:bold;border-radius:4px;padding:4px;background:#BDBDBD;">Coffee Break</span>
</td>
<td style="text-align:left;">
</td>
</tr>
<tr>
<td style="text-align:left;width: 7em; ">
14:30 - 15:00
</td>
<td style="text-align:left;width: 16em; ">
<span style="margin-right:5px;">Conclusion</span>
</td>
<td style="text-align:left;">
Johan Reimegård
</td>
</tr>
</tbody>
</table>
<!-- marker legend -->
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2 changes: 1 addition & 1 deletion 2403-canvas/index.html
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Expand Up @@ -201,7 +201,7 @@ <h1>Analysis of RNA-Seq Data</h1>
<hr />
<div class="landing-update">
<p class="small" style="color:#bdbdbd;">
Last updated on <svg aria-hidden="true" role="img" viewBox="0 0 448 512" style="height:1em;width:0.88em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:#bdbdbd;overflow:visible;position:relative;"><path d="M152 24c0-13.3-10.7-24-24-24s-24 10.7-24 24V64H64C28.7 64 0 92.7 0 128v16 48V448c0 35.3 28.7 64 64 64H384c35.3 0 64-28.7 64-64V192 144 128c0-35.3-28.7-64-64-64H344V24c0-13.3-10.7-24-24-24s-24 10.7-24 24V64H152V24zM48 192h80v56H48V192zm0 104h80v64H48V296zm128 0h96v64H176V296zm144 0h80v64H320V296zm80-48H320V192h80v56zm0 160v40c0 8.8-7.2 16-16 16H320V408h80zm-128 0v56H176V408h96zm-144 0v56H64c-8.8 0-16-7.2-16-16V408h80zM272 248H176V192h96v56z"/></svg> 07-Mar-2024 at <svg aria-hidden="true" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:#bdbdbd;overflow:visible;position:relative;"><path d="M464 256A208 208 0 1 1 48 256a208 208 0 1 1 416 0zM0 256a256 256 0 1 0 512 0A256 256 0 1 0 0 256zM232 120V256c0 8 4 15.5 10.7 20l96 64c11 7.4 25.9 4.4 33.3-6.7s4.4-25.9-6.7-33.3L280 243.2V120c0-13.3-10.7-24-24-24s-24 10.7-24 24z"/></svg> 09:21:21 PM .
Last updated on <svg aria-hidden="true" role="img" viewBox="0 0 448 512" style="height:1em;width:0.88em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:#bdbdbd;overflow:visible;position:relative;"><path d="M152 24c0-13.3-10.7-24-24-24s-24 10.7-24 24V64H64C28.7 64 0 92.7 0 128v16 48V448c0 35.3 28.7 64 64 64H384c35.3 0 64-28.7 64-64V192 144 128c0-35.3-28.7-64-64-64H344V24c0-13.3-10.7-24-24-24s-24 10.7-24 24V64H152V24zM48 192h80v56H48V192zm0 104h80v64H48V296zm128 0h96v64H176V296zm144 0h80v64H320V296zm80-48H320V192h80v56zm0 160v40c0 8.8-7.2 16-16 16H320V408h80zm-128 0v56H176V408h96zm-144 0v56H64c-8.8 0-16-7.2-16-16V408h80zM272 248H176V192h96v56z"/></svg> 08-Mar-2024 at <svg aria-hidden="true" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-left:auto;margin-right:auto;font-size:inherit;fill:#bdbdbd;overflow:visible;position:relative;"><path d="M464 256A208 208 0 1 1 48 256a208 208 0 1 1 416 0zM0 256a256 256 0 1 0 512 0A256 256 0 1 0 0 256zM232 120V256c0 8 4 15.5 10.7 20l96 64c11 7.4 25.9 4.4 33.3-6.7s4.4-25.9-6.7-33.3L280 243.2V120c0-13.3-10.7-24-24-24s-24 10.7-24 24z"/></svg> 05:31:47 AM .
</p>
</div>
</div>
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26 changes: 13 additions & 13 deletions 2403-canvas/lab_r.html
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Expand Up @@ -405,20 +405,20 @@ <h1><span class="header-section-number">6</span> Functions</h1>

# sort
sort(x)</code></pre>
<pre><code>## [1] 17 184 105 149 82 52 195 39 15 65
<pre><code>## [1] 29 160 183 26 78 162 194 67 196 185
## [1] 10
## [1] 903
## [1] 90.3
## [1] 73.5
## [1] 15
## [1] 2.833213 5.214936 4.653960 5.003946 4.406719 3.951244 5.273000 3.663562
## [9] 2.708050 4.174387
## [1] 2.415495e+07 8.131762e+79 3.989520e+45 5.127171e+64 4.093997e+35
## [6] 3.831008e+22 4.868823e+84 8.659340e+16 3.269017e+06 1.694889e+28
## [1] 4.123106 13.564660 10.246951 12.206556 9.055385 7.211103 13.964240
## [8] 6.244998 3.872983 8.062258
## [1] 17 184 105 149 82 52 195 39 15 65
## [1] 15 17 39 52 65 82 105 149 184 195</code></pre>
## [1] 1280
## [1] 128
## [1] 161
## [1] 26
## [1] 3.367296 5.075174 5.209486 3.258097 4.356709 5.087596 5.267858 4.204693
## [9] 5.278115 5.220356
## [1] 3.931334e+12 3.069850e+69 2.991508e+79 1.957296e+11 7.498417e+33
## [6] 2.268329e+70 1.791140e+84 1.252363e+29 1.323483e+85 2.210442e+80
## [1] 5.385165 12.649111 13.527749 5.099020 8.831761 12.727922 13.928388
## [8] 8.185353 14.000000 13.601471
## [1] 29 160 183 26 78 162 194 67 196 185
## [1] 26 29 67 78 160 162 183 185 194 196</code></pre>
<p>Some useful string functions.</p>
<pre class="r"><code>a &lt;- &quot;sunny&quot;
b &lt;- &quot;day&quot;
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17 changes: 8 additions & 9 deletions 2403-canvas/slide_clustering.html
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- Dependent on number of K (clusters) new centroids are created

&lt;!-- &lt;div style="text-align: center;"&gt; --&gt;
&lt;!-- &lt;img src="data/kmeans.gif" alt="Alt text for the GIF" style="width: 65%; height: auto;"&gt; --&gt;
&lt;!-- &lt;img src="data/kmeans_3.gif" alt="Alt text for the GIF" style="width: 65%; height: auto;"&gt; --&gt;
&lt;!-- &lt;/div&gt; --&gt;
![](data/kmeans_DS.gif)
---
name: Centroid-based2
![](data/Kmeans_3_DS.gif){width=50%}


---
name: Centroid-based2
name: Centroid-based3
## Centroid-based: K-means clustering
- One of the most commonly used clustering methods

Expand All @@ -148,15 +150,12 @@
- Each data point is assigned to a cluster based on Euclidean distance from centroid.

- Dependent on number of K (clusters) new centroids are created

&lt;!-- &lt;div style="text-align: center;"&gt; --&gt;
&lt;!-- &lt;img src="data/kmeans_3.gif" alt="Alt text for the GIF" style="width: 65%; height: auto;"&gt; --&gt;
&lt;!-- &lt;/div&gt; --&gt;
![](data/Kmeans_3_DS.gif)
---
name: Centroid-based4
![](data/kmeans_DS.gif){width=50%}


---

name: optimal k
## What is optimal K?

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<head>
<title>Differential Gene Expression</title>
<meta charset="utf-8" />
<meta name="author" content="Julie Lorent | 07-Mar-2024" />
<meta name="author" content="Julie Lorent | 08-Mar-2024" />
<meta name="keywords" content="bioinformatics, course, scilifelab, nbis" />
<script src="site_libs/header-attrs-2.21/header-attrs.js"></script>
<link href="site_libs/font-awesome-6.4.2/css/all.min.css" rel="stylesheet" />
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## Workshop on RNA-Seq
]
.author[
### <b>Julie Lorent</b> | 07-Mar-2024
### <b>Julie Lorent</b> | 08-Mar-2024
]
.institute[
### NBIS, SciLifeLab
Expand Down Expand Up @@ -294,7 +294,7 @@
.end-text[
&lt;p class="smaller"&gt;
&lt;span class="small" style="line-height: 1.2;"&gt;Graphics from &lt;/span&gt;&lt;img src="./assets/freepik.jpg" style="max-height:20px; vertical-align:middle;"&gt;&lt;br&gt;
Created: 07-Mar-2024 • &lt;a href="https://www.scilifelab.se/"&gt;SciLifeLab&lt;/a&gt; • &lt;a href="https://nbis.se/"&gt;NBIS&lt;/a&gt;
Created: 08-Mar-2024 • &lt;a href="https://www.scilifelab.se/"&gt;SciLifeLab&lt;/a&gt; • &lt;a href="https://nbis.se/"&gt;NBIS&lt;/a&gt;
&lt;/p&gt;
]

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.end-text[
&lt;p class="smaller"&gt;
&lt;span class="small" style="line-height: 1.2;"&gt;Graphics from &lt;/span&gt;&lt;img src="./assets/freepik.jpg" style="max-height:20px; vertical-align:middle;"&gt;&lt;br&gt;
Created: 07-Mar-2024 • &lt;a href="https://www.scilifelab.se/"&gt;SciLifeLab&lt;/a&gt; • &lt;a href="https://nbis.se/"&gt;NBIS&lt;/a&gt;
Created: 08-Mar-2024 • &lt;a href="https://www.scilifelab.se/"&gt;SciLifeLab&lt;/a&gt; • &lt;a href="https://nbis.se/"&gt;NBIS&lt;/a&gt;
&lt;/p&gt;
]

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42 changes: 42 additions & 0 deletions 2403/Dockerfile
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@@ -0,0 +1,42 @@
# DOCKER FILE FOR WORKSHOP-RNASEQ
# 2023 Roy Francis

FROM rocker/verse:4.2.3
LABEL Description="Docker image for NBIS workshop-rnaseq"
LABEL Maintainer="roy.francis@nbis.se"
LABEL org.opencontainers.image.source="https://github.com/NBISweden/workshop-rnaseq"

RUN apt-get update -y \
&& apt-get install --no-install-recommends -y \
libxml2-dev \
libssl-dev \
libcurl4-openssl-dev \
libudunits2-dev \
libopenblas-base \
libgdal-dev \
&& wget https://dl.google.com/linux/direct/google-chrome-stable_current_amd64.deb \
&& apt-get install -y ./google-chrome-stable_current_amd64.deb \
&& rm -rf google-chrome-stable_current_amd64.deb \
&& rm -rf /var/lib/apt/lists/* \
&& Rscript -e 'install.packages(c("bookdown","knitr","remotes","fontawesome","formattable","kableExtra","leaflet","pagedown","lubridate","markdown","xaringan","yaml"),repos = "https://packagemanager.posit.co/cran/__linux__/jammy/latest");' \
&& mkdir /rmd

RUN Rscript -e 'install.packages(c("BiocManager","dplyr","enrichR","ggplot2","gridExtra","pheatmap","pvclust","rafalib","stringr","tidyr","locfit"),repos = "https://packagemanager.posit.co/cran/__linux__/jammy/latest");'
RUN Rscript -e 'BiocManager::install(c("DESeq2","edgeR","fgsea","biomaRt","GEOquery"));'
WORKDIR /rmd
CMD Rscript -e "rmarkdown::render_site()"

# build container
# docker build -t ghcr.io/nbisweden/workshop-rnaseq:1.0.0 .
# docker tag ghcr.io/nbisweden/workshop-rnaseq:1.0.0 ghcr.io/nbisweden/workshop-rnaseq:latest

# push to ghcr
# docker login ghcr.io
# docker push ghcr.io/nbisweden/workshop-rnaseq:1.0.0
# docker push ghcr.io/nbisweden/workshop-rnaseq:latest

# run container
# render all files
# docker run --rm -u $(id -u ${USER}):$(id -g ${USER}) -v ${PWD}:/rmd ghcr.io/nbisweden/workshop-rnaseq:latest
# render one file
# docker run --rm -u $(id -u ${USER}):$(id -g ${USER}) -v ${PWD}:/rmd ghcr.io/nbisweden/workshop-rnaseq:latest Rscript -e 'rmarkdown::render("index.Rmd")'
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35 changes: 35 additions & 0 deletions 2403/assets/footer-lab.Rmd
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---
title: "&nbsp;"
---

<footer class="footer">
<div class="container footer-container">
<div class="row">

<hr>

<div class="col-sm-8" style="text-align:left;vertical-align:middle;">
<p class="small" style="color:#bdbdbd;">
[NBIS](https://nbis.se/)[SciLifeLab](https://www.scilifelab.se/)
</p>
</div>

<div class="col-sm-4" style="text-align:right;vertical-align:middle;">
<p style="color:#bdbdbd;">
<span>
<span class="footericon" style="padding-right:4px; padding-left:4px">
<a href="https://nbis.se/">`r fontawesome::fa("globe-americas",fill="#bdbdbd")`</a>
</span>
<span class="footericon" style="padding-right:4px; padding-left:4px">
<a href="https://twitter.com/NBISwe">`r fontawesome::fa("twitter",fill="#bdbdbd")`</a>
</span>
<span class="footericon" style="padding-left:4px">
<a href="https://www.linkedin.com/company/nbisweden/">`r fontawesome::fa("linkedin",fill="#bdbdbd")`</a>
</span>
</span>
</p>
</div>

</div>
</div>
</footer>
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