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Update README.md
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KJeynesCupper authored Jul 12, 2023
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Expand Up @@ -32,7 +32,7 @@ each sample replicates to a merge genome reference comprised of both genome
assemblies relating to the genotypes in the heterograft system.

**Below is a quick-start guide to the identification of mobile sRNAs in a
chimeric system**
chimeric system** <br>
To undertake sRNAseq analysis for treatment vs control, please see vignettes.


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For either analysis, there are also optional extras which can be explores in the
vignettes. For an advanced analysis of mobile sRNAs in chimeric systems,
view the "mobileRNA" - "Identify sRNAseq data to locate potentially mobile sRNAs
between genotypes using mobileRNA" vignette. While, for an advanced analysis of
view the `mobileRNA - Identify sRNAseq data to locate potentially mobile sRNAs
between genotypes using mobileRNA` vignette. While, for an advanced analysis of
sRNA populations in non-grafted or grafted system with the same species, view
"mobileRNA_standard" - "Analyse sRNA populations using mobileRNA" vignette.
`mobileRNA_standard - Analyse sRNA populations using mobileRNA` vignette.

Going forward,we assume standard quality control steps on raw samples has been
completed (i.e. trimming of adapters and low quality reads)
Expand Down Expand Up @@ -416,7 +416,8 @@ The package also includes functions for:
* Plot the distribution of sRNA classes (20-24nt) across individual replicates or across the dataset.
* Statistical analysis using differential methods from either DESeq2 or edgeR.

The package workflow can easily be manipulated to enable the identification of local populations of RNA species.
**The package workflow can easily be manipulated to enable the identification of local populations of RNA species.**

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