Supplementary data and scripts for the research article "Peripheral Immune Landscape for Hypercytokinemia in Myasthenic Crisis Utilizing Single-cell Transcriptomics".
The PBMCs were loaded onto the 10× Chromium Single Cell Platform (10X Genomics) (5' and V(D)J Enrichment) as described in the manufacturer’s protocol. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification, and library construction were all performed as per the manufacturer’s protocol. Qubit was used for library quantification before pooling. The final library pool was sequenced on the Illumina NovaSeq 6000 using 150 base-pair paired-end reads. The cellranger pipeline (version 6.1.1) was applied to analyze the sequencing raw data, with references of the transcriptome (GRCh38-3.0.0) and VDJ repertoire (vdj_GRCh38_alts_ensembl-4.0.0).
The processed data generated in this study have been deposited in the release of this repository and the Gene Expression Omnibus (GEO) database (GSE222427). The raw sequence data generated in this study have been deposited in the National Omics Data Encyclopaedia database of the Bio-Med Big Data Center, Chinese Academy of Sciences, under the accession code HRA003797. The raw sequence data are only available under restricted access due to data privacy law. Access to the data can be obtained by making a reasonable request to the corresponding authors.
Zhong H, Huan X, Zhao R, Su M, Yan C, Song J, Xi J, Zhao C, Luo F, Luo S. Peripheral immune landscape for hypercytokinemia in myasthenic crisis utilizing single-cell transcriptomics. J Transl Med. 2023 Aug 24;21(1):564. doi: 10.1186/s12967-023-04421-y. PMID: 37620910