CANControl

Matlab software used for controlling Zeiss LSM 510 META and Zeiss AxioVert 200M via Serial Port

General

The devices are controlled via Serial communication. The correct code words to control them can be acquired with 'Free Serial Port Monitor' by sniffing the communication between 'CanCheck' or 'ZEN' and the devices to control. The already implemented functions are described in the next section.

The Matlab class for controlling the devices is named CANControl.

Devices and Functions

Stand Axiovert 200:Focus

This device controls the position of the objective lens of the AxioVert 200M and is adressed using the prefix F. You can get and set the current position and start and stop a continuous scan with a given velocity.

Commands:

• FPZp Request the current position. The read buffer has to be written out in order to get the position in hexadecimal units.
• FPZG1 Set the velocity of the continuous scan as hexadecimal value. A value of 3E8 (1000) amounts to approximately 1.5 µm per second.
• FPZS- Start a scan in negative direction.
• FPZS+ Start a scan in positive direction.
• FPZSS Stop the scan.
• FPZDxxxxxx Set the current position with xxxxxx as the desired position in hexadecimal units. One increment corresponds to 0.025 µm.
• FPZt State of scanner. 0 corresponds to static focus, 255 means moving lens.
• FPw Status key of the current position. 01 corresponds to the work position, 04 means load position.
• FPW0 Moves the focus to the load position.
• FPW1 Moves the focus to the work position.

Stand Axiovert 200:Stand

This device controls the objective, reflector, sideport etc. of the AxioVert 200M and is adressed using the prefix H. You can set the positions of the devices.

Commands:

• HPCR1,x Set the position of the Reflector. The value x is the desired position with values [1, 2, 3, 4, 5].
• HPCr1,1 Get the position of the Reflector. Returns PHx with x as the position of the Reflector.
• HPCR2,x Set the position of the Objective. The value x is the desired position with values [1, 2, 3, 4, 5, 6].
• HPCr2,1 Get the position of the Objective. Returns PHx with x as the position of the Objective.
• HPCR36,x Set the position of the Tubelens. The value x is the desired position with values [1, 2, 3].
• HPCr36,1 Get the position of the Tubelens. Returns PHx with x as the position of the Tubelens.
• HPCR38,x Set the position of the Baseport. The value x is the desired position with values [1, 2, 3].
• HPCr38,1 Get the position of the Baseport. Returns PHx with x as the position of the Baseport.
• HPCR39,x Set the position of the Sideport. The value x is the desired position with values [1, 2, 3].
• HPCr39,1 Get the position of the Sideport. Returns PHx with x as the position of the Sideport.
• HPCR51,x Set the position of the Mirror. The value x is the desired position with values [1, 2].
• HPCr51,1 Get the position of the Mirror. Returns PHx with x as the position of the Mirror.

There are more commands which are not necessary for the Axiovert 200M in Room 340.

LSM 510:Scanhead

This device controls the laser scanning module and is adressed using the prefix I. You can control beam splitters, collimators, filters and pinholes.

Beam splitters

Commands:

• IPCr6,1 Get the position of the main beam splitter (HT). Returns PIxxx with xxx as the position of HT.
• IPCR6,x Set the position of HT. The value x is the desired position with values [25, 19, 13, 7, 1, 43, 37, 31] corresponding to the positions [1, 2, 3, 4, 5, 6, 7, 8] of HT.
• IPCr7,1 Get the position of the secondary beam splitter 1 (NT1). Returns PIxxx with ```xxx`` as the position of NT1.
• IPCR7,x Set the position of NT1. The value x is the desired position with values [25, 19, 13, 7, 1, 43, 37, 31] corresponding to the positions [1, 2, 3, 4, 5, 6, 7, 8] of NT1.
• IPCr8,1 Get the position of the secondary beam splitter 2 (NT2). Returns PIxxx with xxx as the position of NT2.
• IPCR8,x Set the position of NT2. The value x is the desired position with values [10, 1, 28, 19] corresponding to the positions [1, 2, 3, 4] of NT2.
• IPCr9,1 Get the position of the secondary beam splitter 3 (NT3). Returns PIxxx with xxx as the position of NT3.
• IPCR9,x Set the position of NT3. The value x is the desired position with values [10, 1, 28, 19] corresponding to the positions [1, 2, 3, 4] of NT3.

Filters

Commands:

• IPCr11,1 Get the position of filter wheel 2 (EF2). Returns PIxxx with xxx as the position of EF2.
• IPCR11,x Set the position of EF2. The value x is the desired position with values [25, 31, 37, 43, 1, 7, 13, 19] corresponding to the positions [1, 2, 3, 4, 5, 6, 7, 8] of EF2.
• IPCr12,1 Get the position of filter wheel 3 (EF3). Returns PIxxx with xxx as the position of EF3.
• IPCR12,x Set the position of EF3. The value x is the desired position with values [18, 24, 30, 36, 42, 48, 6, 12, 18] corresponding to the positions [1, 2, 3, 4, 5, 6, 7, 8] of EF3.
• IPCr14,1 Get the position of filter wheel 5 (EF5). Returns PIxxx with xxx as the position of EF5.
• IPCR14,x Set the position of EF5. The value x is the desired position with values [1, 7, 13, 19, 25, 31, 37, 43] corresponding to the positions [1, 2, 3, 4, 5, 6, 7, 8] of EF5.

Pinholes

Commands:

• IPCs16,1 Get the x-position of pinhole 1 (PH1). Returns PIxxx with xxx as the position of PH1.

• IPCS16,x Set the x-position of PH1. The value x is the desired position with values from 1 to 250.

• IPCs20,1 Get the y-position of PH1. Returns PIxxx with xxx as the position of PH1.

• IPCS20,x Set the y-position of PH1. The value x is the desired position with values from 1 to 250.

• IPCs24,1 Get the z-position of PH1. Returns PIxxx with xxx as the position of PH1.

• IPCS24,x Set the z-position of PH1. The value x is the desired position with values from 1 to 36.

• IPCs27,1 Get the diameter of PH1. Returns PIxxx with xxx as the diameter of PH1.

• IPCS27,x Set the diameter of PH1. The value x is the desired diameter with values from 1 to 250.

• IPCs17,1 Get the x-position of pinhole 2 (PH2). Returns PIxxx with xxx as the position of PH2.

• IPCS17,x Set the x-position of PH2. The value x is the desired position with values from 1 to 250.

• IPCs21,1 Get the y-position of PH2. Returns PIxxx with xxx as the position of PH2.

• IPCS21,x Set the y-position of PH2. The value x is the desired position with values from 1 to 250.

• IPCs28,1 Get the diameter of PH2. Returns PIxxx with xxx as the diameter of PH2.

• IPCS28,x Set the diameter of PH2. The value x is the desired diameter with values from 1 to 250.

• IPCs18,1 Get the x-position of pinhole 3 (PH3). Returns PIxxx with xxx as the position of PH3.

• IPCS18,x Set the x-position of PH3. The value x is the desired position with values from 1 to 250.

• IPCs22,1 Get the y-position of PH3. Returns PIxxx with xxx as the position of PH3.

• IPCS22,x Set the y-position of PH3. The value x is the desired position with values from 1 to 250.

• IPCs29,1 Get the diameter of PH3. Returns PIxxx with xxx as the diameter of PH3.

• IPCS29,x Set the diameter of PH3. The value x is the desired diameter with values from 1 to 250.

• IPCs19,1 Get the x-position of pinhole 4 (PH4). Returns PIxxx with xxx as the position of PH4.

• IPCS19,x Set the x-position of PH4. The value x is the desired position with values from 1 to 250.

• IPCs23,1 Get the y-position of PH4. Returns PIxxx with xxx as the position of PH4.

• IPCS23,x Set the y-position of PH4. The value x is the desired position with values from 1 to 250.

• IPCs30,1 Get the diameter of PH4. Returns PIxxx with xxx as the diameter of PH4.

• IPCS30,x Set the diameter of PH4. The value x is the desired diameter with values from 1 to 250.

Collimators

Commands:

• IPCs25,1 Get the position of collimator 1. Returns PIxxx with xxx as the position of collimator 1.
• IPCS25,x Set the position of collimator 1. The value x is the desired position with values from 1to 120.
• IPCs26,1 Get the position of collimator 2. Returns PIxxx with xxx as the position of collimator 2.
• IPCS26,x Set the position of collimator 2. The value x is the desired position with values from 1to 110.

LSM 510:Meta

This device controls the META detector and is adressed using the prefix C. You can control the grating position.

Commands:

• CPCs26,1 Get the position of the grating 1 (GS1). Returns PCxxx with xxx as the position of GS1.
• CPCS26,x Set the position of GS1. The value x is the desired position with values from 1 to 600.

Stage 28:MCU 28

This device controls the x- and y-position of the translation stage and is adressed using the prefix N.

Commands:

• NPXp Get the x-position. Returns PNxxxxxx with xxxxxx as the x-position of the stage.
• NPYp Get the y-position. Returns PNxxxxxx with xxxxxx as the y-position of the stage.
• NPXS Stops the movement of the x-axis.
• NPYS Stops the movement of the y-axis.
• NPXVx Sets the velocity of the x-axis to the decimal value x. A value of 1 corresponds to a velocity of approximately 500 µm per second.
• NPYVx Sets the velocity of the y-axis to the decimal value x. A value of 1 corresponds to a velocity of approximately 500 µm per second.
• NPXt State of x-axis. 0 corresponds to a static stage, 255 means moving stage.
• NPYt State of y-axis. 0 corresponds to a static stage, 255 means moving stage.
• NPXmx Purpose unknown. Accepts value from 0 to 9 for x, returns either 0 or 1.
• NPYmx Purpose unknown. Accepts value from 0 to 9 for x, returns either 0 or 1.
• NPXTxxxxxx Set the x-position of the stage. The value xxxxxx is the desired position.
• NPYTxxxxxx Set the y-position of the stage. The value xxxxxx is the desired position.