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Experimental BD Rhapsody Support
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Experimental BD Rhapsody Support based on COMBINE-lab/salmon#628 (comment)
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ACastanza committed Feb 26, 2021
1 parent 814504f commit e8d451f
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Showing 2 changed files with 6 additions and 2 deletions.
4 changes: 2 additions & 2 deletions manifest
Original file line number Diff line number Diff line change
Expand Up @@ -174,7 +174,7 @@ p3_value=
p4_MODE=
p4_TYPE=TEXT
p4_default_value=dropseq
p4_description=The chemistry used by the single-cell sequencing platform.<br>\nDrop-Seq, 10x Chromium v2, 10x Chromium v3, CITE-Seq, CEL-Seq, CEL-Seq2, and Quartz-Seq2 are currently supported.
p4_description=The chemistry used by the single-cell sequencing platform.<br>\nDrop-Seq, 10x Chromium v2, 10x Chromium v3, CITE-Seq, CEL-Seq, CEL-Seq2, and Quartz-Seq2 are currently supported. BD Rhapsody support is experimental.
p4_fileFormat=
p4_flag=-c
p4_name=Chemistry
Expand All @@ -183,7 +183,7 @@ p4_optional=
p4_prefix=
p4_prefix_when_specified=
p4_type=java.lang.String
p4_value=dropseq\=Drop-Seq;chromium\=10x Chromium v2;chromiumV3\=10x Chromium v3;citeseq\=CITE-Seq;celseq\=CEL-Seq;celseq2\=CEL-Seq2;quartzseq2\=Quartz-Seq2
p4_value=dropseq\=Drop-Seq;chromium\=10x Chromium v2;chromiumV3\=10x Chromium v3;citeseq\=CITE-Seq;celseq\=CEL-Seq;celseq2\=CEL-Seq2;quartzseq2\=Quartz-Seq2;rhapsody\=BD Rhapsody (Experimental)
p5_MODE=
p5_TYPE=TEXT
p5_default_value=ISR
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4 changes: 4 additions & 0 deletions salmon_alevin_quant.sh
Original file line number Diff line number Diff line change
Expand Up @@ -255,6 +255,10 @@ params=()
[[ $method == "celseq2" ]] && params+=(--celseq2)
[[ $method == "quartzseq2" ]] && params+=(--quartzseq2)

[[ $method == "rhapsody" ]] && params+=(--umi-geometry \'1[53-60]\')
[[ $method == "rhapsody" ]] && params+=(--bc-geometry \'1[1-9,22-30,44-52]\')
[[ $method == "rhapsody" ]] && params+=(--read-geometry \'2[1-end]\')

[[ -e "$whitelist" ]] && params+=(--whitelist $whitelist)
[[ -e "$mtrna" ]] && params+=(--mrna $mtrna)
[[ -e "$rrna" ]] && params+=(--rrna $rrna)
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