diff --git a/packages/browser/src/node/plugin.ts b/packages/browser/src/node/plugin.ts
index 524fb9bf3307..a96da95ce8d1 100644
--- a/packages/browser/src/node/plugin.ts
+++ b/packages/browser/src/node/plugin.ts
@@ -168,7 +168,6 @@ export default (browserServer: BrowserServer, base = '/'): Plugin[] => {
         const define: Record<string, string> = {}
         for (const env in (project.config.env || {})) {
           const stringValue = JSON.stringify(project.config.env[env])
-          define[`process.env.${env}`] = stringValue
           define[`import.meta.env.${env}`] = stringValue
         }
 
@@ -388,24 +387,10 @@ export default (browserServer: BrowserServer, base = '/'): Plugin[] => {
         }
       },
     },
-    // TODO: remove this when @testing-library/vue supports ESM
     {
       name: 'vitest:browser:support-testing-library',
       config() {
         return {
-          define: {
-            // testing-library/preact
-            'process.env.PTL_SKIP_AUTO_CLEANUP': !!process.env.PTL_SKIP_AUTO_CLEANUP,
-            // testing-library/react
-            'process.env.RTL_SKIP_AUTO_CLEANUP': !!process.env.RTL_SKIP_AUTO_CLEANUP,
-            'process.env?.RTL_SKIP_AUTO_CLEANUP': !!process.env.RTL_SKIP_AUTO_CLEANUP,
-            // testing-library/svelte, testing-library/solid
-            'process.env.STL_SKIP_AUTO_CLEANUP': !!process.env.STL_SKIP_AUTO_CLEANUP,
-            // testing-library/vue
-            'process.env.VTL_SKIP_AUTO_CLEANUP': !!process.env.VTL_SKIP_AUTO_CLEANUP,
-            // dom.debug()
-            'process.env.DEBUG_PRINT_LIMIT': process.env.DEBUG_PRINT_LIMIT || 7000,
-          },
           optimizeDeps: {
             esbuildOptions: {
               plugins: [
diff --git a/test/browser/test/another.test.ts b/test/browser/test/another.test.ts
index 1e66b9ab7ea2..31e0886d7897 100644
--- a/test/browser/test/another.test.ts
+++ b/test/browser/test/another.test.ts
@@ -3,3 +3,7 @@ import { expect, it } from 'vitest'
 it('basic 3', async () => {
   expect(globalThis.window).toBeDefined()
 })
+
+it('no process', () => {
+  expect(globalThis.process).toBeUndefined()
+})