diff --git a/packages/vite/src/node/optimizer/scan.ts b/packages/vite/src/node/optimizer/scan.ts
index b28c13167e5fb6..836bfb2a5f8c3a 100644
--- a/packages/vite/src/node/optimizer/scan.ts
+++ b/packages/vite/src/node/optimizer/scan.ts
@@ -242,7 +242,7 @@ function globEntries(pattern: string | string[], config: ResolvedConfig) {
   return glob(pattern, {
     cwd: config.root,
     ignore: [
-      '**/node_modules/**',
+      glob.convertPathToPattern(config.root) + '/**/node_modules/**',
       `**/${config.build.outDir}/**`,
       // if there aren't explicit entries, also ignore other common folders
       ...(config.optimizeDeps.entries