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Prokka blast version issue? #717

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theblendermann opened this issue Jan 16, 2025 · 1 comment
Open

Prokka blast version issue? #717

theblendermann opened this issue Jan 16, 2025 · 1 comment

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@theblendermann
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theblendermann commented Jan 16, 2025

When running prokka version 1.13, it says prokka requires Blastp 2.2 or higher, while also saying my Blastp version is 2.12.

This is prokka 1.13
Written by Torsten Seemann <torsten.seemann@gmail.com>
Homepage is https://github.com/tseemann/prokka
Local time is Thu Jan 16 21:28:49 2025
You are a_user
Operating system is linux
You have BioPerl 1.007002
System has 20 cores.
Will use maximum of 6 cores.
Annotating as >>> Bacteria <<<
Generating locus_tag from 'a_user's_organism.fna' contents.
Creating new output folder: Prokka_Annotation
Running: mkdir -p Prokka_Annotation
Using filename prefix: PROKKA_01162025.XXX
Setting HMMER_NCPU=1
Writing log to: Prokka_Annotation/PROKKA_01162025.log
Command: /home/a_user/miniconda3/envs/prokka/bin/prokka --outdir Prokka_Annotation --cpus 6 --rnammer --genus Thermus --species aquaticus --kingdom Bacteria 'a_user's_organism.fna'
Appending to PATH: /home/a_user/miniconda3/envs/prokka/bin
Looking for 'aragorn' - found /home/a_user/miniconda3/envs/prokka/bin/aragorn
Determined aragorn version is 1.2
Looking for 'barrnap' - found /home/a_user/miniconda3/envs/prokka/bin/barrnap
Determined barrnap version is 0.8
Looking for 'blastp' - found /home/a_user/miniconda3/envs/prokka/bin/blastp
Determined blastp version is 2.12
Prokka needs blastp 2.2 or higher. Please upgrade and try again.
@gouravreddy02
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version 1.13 had a bug where it says prokka requires blast 2.2 or higher when blast version is 2.16, but it is fixed when we install the latest version. A temporary fix if you want to use the previous version is to change MINVER of blastp and makeblastdb to 2.1 in prokka script

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