This is a software implementation of the smFRET kinetics analysis method described in the Journal of Chemical Physics article “Kinetic analysis of single molecule FRET transitions without trajectories” (1), which was used to perform the data anlysis for the article.
The documentation can be found at https://schuetzgroup.github.io/fret-tester/. There is also a Jupyter notebook containing a tutorial.
- Python 3 (tested on version 3.6)
- numpy (tested on version 1.13)
- scipy (tested on version 1.0)
- matplotlib
- Download source (either use
git cloneor download a snapshot zip and unpack it somewhere). - Go to the topmost folder of the source and run
python setup.py installor add the topmost folder to the Python path.
(1) Schrangl, Lukas; Göhring, Janett; Schütz, Gerhard J. (2018): “Kinetic analysis of single molecule FRET transitions without trajectories.” In: The Journal of Chemical Physics, 148 (2018), H. 12, p. 123328. Available at: DOI: 10.1063/1.5006038