diff --git a/Tests/test_image_access.py b/Tests/test_image_access.py
index 4079d935800..027af5d56fa 100644
--- a/Tests/test_image_access.py
+++ b/Tests/test_image_access.py
@@ -275,15 +275,10 @@ def test_get_vs_c(self):
         # self._test_get_access(hopper('PA')) # PA -- how do I make a PA image?
         self._test_get_access(hopper("F"))
 
-        im = Image.new("I;16", (10, 10), 40000)
-        self._test_get_access(im)
-        im = Image.new("I;16L", (10, 10), 40000)
-        self._test_get_access(im)
-        im = Image.new("I;16B", (10, 10), 40000)
-        self._test_get_access(im)
-
-        im = Image.new("I", (10, 10), 40000)
-        self._test_get_access(im)
+        for mode in ("I;16", "I;16L", "I;16B", "I;16N", "I"):
+            im = Image.new(mode, (10, 10), 40000)
+            self._test_get_access(im)
+
         # These don't actually appear to be modes that I can actually make,
         # as unpack sets them directly into the I mode.
         # im = Image.new('I;32L', (10, 10), -2**10)
@@ -322,15 +317,10 @@ def test_set_vs_c(self):
         # self._test_set_access(i, (128, 128))  #PA  -- undone how to make
         self._test_set_access(hopper("F"), 1024.0)
 
-        im = Image.new("I;16", (10, 10), 40000)
-        self._test_set_access(im, 45000)
-        im = Image.new("I;16L", (10, 10), 40000)
-        self._test_set_access(im, 45000)
-        im = Image.new("I;16B", (10, 10), 40000)
-        self._test_set_access(im, 45000)
+        for mode in ("I;16", "I;16L", "I;16B", "I;16N", "I"):
+            im = Image.new(mode, (10, 10), 40000)
+            self._test_set_access(im, 45000)
 
-        im = Image.new("I", (10, 10), 40000)
-        self._test_set_access(im, 45000)
         # im = Image.new('I;32L', (10, 10), -(2**10))
         # self._test_set_access(im, -(2**13)+1)
         # im = Image.new('I;32B', (10, 10), 2**10)
diff --git a/Tests/test_lib_pack.py b/Tests/test_lib_pack.py
index 979806cae99..de3e7d1569b 100644
--- a/Tests/test_lib_pack.py
+++ b/Tests/test_lib_pack.py
@@ -207,6 +207,9 @@ def test_I(self):
                 0x01000083,
             )
 
+    def test_I16(self):
+        self.assert_pack("I;16N", "I;16N", 2, 0x0201, 0x0403, 0x0605)
+
     def test_F_float(self):
         self.assert_pack("F", "F;32F", 4, 1.539989614439558e-36, 4.063216068939723e-34)
 
@@ -761,10 +764,12 @@ def test_I16(self):
             self.assert_unpack("I;16", "I;16N", 2, 0x0201, 0x0403, 0x0605)
             self.assert_unpack("I;16B", "I;16N", 2, 0x0201, 0x0403, 0x0605)
             self.assert_unpack("I;16L", "I;16N", 2, 0x0201, 0x0403, 0x0605)
+            self.assert_unpack("I;16N", "I;16N", 2, 0x0201, 0x0403, 0x0605)
         else:
             self.assert_unpack("I;16", "I;16N", 2, 0x0102, 0x0304, 0x0506)
             self.assert_unpack("I;16B", "I;16N", 2, 0x0102, 0x0304, 0x0506)
             self.assert_unpack("I;16L", "I;16N", 2, 0x0102, 0x0304, 0x0506)
+            self.assert_unpack("I;16N", "I;16N", 2, 0x0102, 0x0304, 0x0506)
 
     def test_CMYK16(self):
         self.assert_unpack("CMYK", "CMYK;16L", 8, (2, 4, 6, 8), (10, 12, 14, 16))
diff --git a/Tests/test_mode_i16.py b/Tests/test_mode_i16.py
index dcdee3d416d..1786dba3847 100644
--- a/Tests/test_mode_i16.py
+++ b/Tests/test_mode_i16.py
@@ -88,10 +88,7 @@ def tobytes(mode):
 def test_convert():
     im = original.copy()
 
-    verify(im.convert("I;16"))
-    verify(im.convert("I;16").convert("L"))
-    verify(im.convert("I;16").convert("I"))
-
-    verify(im.convert("I;16B"))
-    verify(im.convert("I;16B").convert("L"))
-    verify(im.convert("I;16B").convert("I"))
+    for mode in ("I;16", "I;16B", "I;16N"):
+        verify(im.convert(mode))
+        verify(im.convert(mode).convert("L"))
+        verify(im.convert(mode).convert("I"))
diff --git a/docs/releasenotes/9.5.0.rst b/docs/releasenotes/9.5.0.rst
index bd6e586932a..13c99313a22 100644
--- a/docs/releasenotes/9.5.0.rst
+++ b/docs/releasenotes/9.5.0.rst
@@ -63,3 +63,10 @@ Added support for saving PDFs in RGBA mode
 ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
 
 Using the JPXDecode filter, PDFs can now be saved in RGBA mode.
+
+
+Improved I;16N support
+^^^^^^^^^^^^^^^^^^^^^^
+
+Support has been added for I;16N access, packing and unpacking. Conversion to
+and from L mode has also been added.
diff --git a/src/PIL/PyAccess.py b/src/PIL/PyAccess.py
index e9cb34ceda1..39747b4f311 100644
--- a/src/PIL/PyAccess.py
+++ b/src/PIL/PyAccess.py
@@ -320,6 +320,7 @@ def set_pixel(self, x, y, color):
     "1": _PyAccess8,
     "L": _PyAccess8,
     "P": _PyAccess8,
+    "I;16N": _PyAccessI16_N,
     "LA": _PyAccess32_2,
     "La": _PyAccess32_2,
     "PA": _PyAccess32_2,
diff --git a/src/libImaging/Access.c b/src/libImaging/Access.c
index 83860c38a7e..f00939da0b3 100644
--- a/src/libImaging/Access.c
+++ b/src/libImaging/Access.c
@@ -13,7 +13,7 @@
 
 /* use make_hash.py from the pillow-scripts repository to calculate these values */
 #define ACCESS_TABLE_SIZE 27
-#define ACCESS_TABLE_HASH 3078
+#define ACCESS_TABLE_HASH 33051
 
 static struct ImagingAccessInstance access_table[ACCESS_TABLE_SIZE];
 
@@ -92,6 +92,12 @@ get_pixel_16B(Imaging im, int x, int y, void *color) {
 #endif
 }
 
+static void
+get_pixel_16(Imaging im, int x, int y, void *color) {
+    UINT8 *in = (UINT8 *)&im->image[y][x + x];
+    memcpy(color, in, sizeof(UINT16));
+}
+
 static void
 get_pixel_32(Imaging im, int x, int y, void *color) {
     memcpy(color, &im->image32[y][x], sizeof(INT32));
@@ -186,6 +192,7 @@ ImagingAccessInit() {
     ADD("I;16", get_pixel_16L, put_pixel_16L);
     ADD("I;16L", get_pixel_16L, put_pixel_16L);
     ADD("I;16B", get_pixel_16B, put_pixel_16B);
+    ADD("I;16N", get_pixel_16, put_pixel_16L);
     ADD("I;32L", get_pixel_32L, put_pixel_32L);
     ADD("I;32B", get_pixel_32B, put_pixel_32B);
     ADD("F", get_pixel_32, put_pixel_32);
diff --git a/src/libImaging/Convert.c b/src/libImaging/Convert.c
index b03bd02af2b..7fe24a63939 100644
--- a/src/libImaging/Convert.c
+++ b/src/libImaging/Convert.c
@@ -990,6 +990,13 @@ static struct {
     {"I;16L", "L", I16L_L},
     {"L", "I;16B", L_I16B},
     {"I;16B", "L", I16B_L},
+#ifdef WORDS_BIGENDIAN
+    {"L", "I;16N", L_I16B},
+    {"I;16N", "L", I16B_L},
+#else
+    {"L", "I;16N", L_I16L},
+    {"I;16N", "L", I16L_L},
+#endif
 
     {"I;16", "F", I16L_F},
     {"I;16L", "F", I16L_F},
diff --git a/src/libImaging/Pack.c b/src/libImaging/Pack.c
index 01760e742be..14c8f1461aa 100644
--- a/src/libImaging/Pack.c
+++ b/src/libImaging/Pack.c
@@ -664,6 +664,7 @@ static struct {
 #endif
     {"I;16B", "I;16B", 16, copy2},
     {"I;16L", "I;16L", 16, copy2},
+    {"I;16N", "I;16N", 16, copy2},
     {"I;16", "I;16N", 16, packI16N_I16},  // LibTiff native->image endian.
     {"I;16L", "I;16N", 16, packI16N_I16},
     {"I;16B", "I;16N", 16, packI16N_I16B},
diff --git a/src/libImaging/Unpack.c b/src/libImaging/Unpack.c
index e426ed74fce..7eeadf944ea 100644
--- a/src/libImaging/Unpack.c
+++ b/src/libImaging/Unpack.c
@@ -1762,6 +1762,7 @@ static struct {
     {"I;16", "I;16", 16, copy2},
     {"I;16B", "I;16B", 16, copy2},
     {"I;16L", "I;16L", 16, copy2},
+    {"I;16N", "I;16N", 16, copy2},
 
     {"I;16", "I;16N", 16, unpackI16N_I16},   // LibTiff native->image endian.
     {"I;16L", "I;16N", 16, unpackI16N_I16},  // LibTiff native->image endian.