diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 207dc9b717c..14718ddcb6b 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -114,6 +114,7 @@ jobs:
           - "3.10"
           - "3.11"
           - "3.12"
+          - "3.13"
 
     steps:
       - uses: actions/checkout@v4
@@ -167,7 +168,8 @@ jobs:
           # - "3.9"
           # - "3.10"
           # - "3.11"
-          - "3.12"
+          - "3.12"  # Comment out when 3.13 is final
+          - "3.13"
         group: [1, 2]
 
     steps:
@@ -175,6 +177,7 @@ jobs:
       - uses: actions/setup-python@v5
         with:
           python-version: ${{ matrix.python }}
+          allow-prereleases: true
 
       # We use C:\Temp (which is already available on the worker)
       # as a temporary directory for all of the tests because the
diff --git a/news/12620.trivial.rst b/news/12620.trivial.rst
new file mode 100644
index 00000000000..52fa571ed17
--- /dev/null
+++ b/news/12620.trivial.rst
@@ -0,0 +1 @@
+Enable Python 3.13 CI tests
diff --git a/noxfile.py b/noxfile.py
index dc74654da53..92d0e243838 100644
--- a/noxfile.py
+++ b/noxfile.py
@@ -67,7 +67,7 @@ def should_update_common_wheels() -> bool:
 # -----------------------------------------------------------------------------
 # Development Commands
 # -----------------------------------------------------------------------------
-@nox.session(python=["3.8", "3.9", "3.10", "3.11", "3.12", "pypy3"])
+@nox.session(python=["3.8", "3.9", "3.10", "3.11", "3.12", "3.13", "pypy3"])
 def test(session: nox.Session) -> None:
     # Get the common wheels.
     if should_update_common_wheels():
diff --git a/tests/requirements.txt b/tests/requirements.txt
index 5ecb21f6bf6..7fba866c745 100644
--- a/tests/requirements.txt
+++ b/tests/requirements.txt
@@ -1,3 +1,4 @@
+cffi @ https://github.com/python-cffi/cffi/archive/refs/heads/main.zip; python_version > "3.12" # Temporary workaround for Python 3.13 until next CFFI release
 cryptography
 freezegun
 installer
diff --git a/tests/unit/test_cache.py b/tests/unit/test_cache.py
index d0fee69c39b..30cdb6ebece 100644
--- a/tests/unit/test_cache.py
+++ b/tests/unit/test_cache.py
@@ -13,11 +13,11 @@ def test_falsey_path_none() -> None:
     assert wc.cache_dir is None
 
 
-def test_subdirectory_fragment() -> None:
+def test_subdirectory_fragment(tmp_path: Path) -> None:
     """
     Test the subdirectory URL fragment is part of the cache key.
     """
-    wc = WheelCache("/tmp/.foo/")
+    wc = WheelCache(os.fspath(tmp_path))
     link1 = Link("git+https://g.c/o/r#subdirectory=d1")
     link2 = Link("git+https://g.c/o/r#subdirectory=d2")
     assert wc.get_path_for_link(link1) != wc.get_path_for_link(link2)