diff --git a/xarray/core/datatree.py b/xarray/core/datatree.py
index 48b08aacebf..e955a2de669 100644
--- a/xarray/core/datatree.py
+++ b/xarray/core/datatree.py
@@ -70,8 +70,11 @@
         ErrorOptions,
         ErrorOptionsWithWarn,
         NetcdfWriteModes,
+        T_ChunkDimFreq,
+        T_ChunksFreq,
         ZarrWriteModes,
     )
+    from xarray.namedarray.parallelcompat import ChunkManagerEntrypoint
 
 # """
 # DEVELOPERS' NOTE
@@ -1997,3 +2000,82 @@ def chunksizes(self) -> Mapping[Hashable, tuple[int, ...]]:
             f"/{path}" if path != "." else "/": get_chunksizes(node.variables.values())
             for path, node in self.subtree_with_keys
         }
+
+    def chunk(
+        self,
+        chunks: T_ChunksFreq = {},  # noqa: B006  # {} even though it's technically unsafe, is being used intentionally here (#4667)
+        name_prefix: str = "xarray-",
+        token: str | None = None,
+        lock: bool = False,
+        inline_array: bool = False,
+        chunked_array_type: str | ChunkManagerEntrypoint | None = None,
+        from_array_kwargs=None,
+        **chunks_kwargs: T_ChunkDimFreq,
+    ) -> Self:
+        """Coerce all arrays in all groups in this tree into dask arrays with the given
+        chunks.
+
+        Non-dask arrays in this tree will be converted to dask arrays. Dask
+        arrays will be rechunked to the given chunk sizes.
+
+        If neither chunks is not provided for one or more dimensions, chunk
+        sizes along that dimension will not be updated; non-dask arrays will be
+        converted into dask arrays with a single block.
+
+        Along datetime-like dimensions, a :py:class:`groupers.TimeResampler` object is also accepted.
+
+        Parameters
+        ----------
+        chunks : int, tuple of int, "auto" or mapping of hashable to int or a TimeResampler, optional
+            Chunk sizes along each dimension, e.g., ``5``, ``"auto"``, or
+            ``{"x": 5, "y": 5}`` or ``{"x": 5, "time": TimeResampler(freq="YE")}``.
+        name_prefix : str, default: "xarray-"
+            Prefix for the name of any new dask arrays.
+        token : str, optional
+            Token uniquely identifying this dataset.
+        lock : bool, default: False
+            Passed on to :py:func:`dask.array.from_array`, if the array is not
+            already as dask array.
+        inline_array: bool, default: False
+            Passed on to :py:func:`dask.array.from_array`, if the array is not
+            already as dask array.
+        chunked_array_type: str, optional
+            Which chunked array type to coerce this datasets' arrays to.
+            Defaults to 'dask' if installed, else whatever is registered via the `ChunkManagerEntryPoint` system.
+            Experimental API that should not be relied upon.
+        from_array_kwargs: dict, optional
+            Additional keyword arguments passed on to the `ChunkManagerEntrypoint.from_array` method used to create
+            chunked arrays, via whichever chunk manager is specified through the `chunked_array_type` kwarg.
+            For example, with dask as the default chunked array type, this method would pass additional kwargs
+            to :py:func:`dask.array.from_array`. Experimental API that should not be relied upon.
+        **chunks_kwargs : {dim: chunks, ...}, optional
+            The keyword arguments form of ``chunks``.
+            One of chunks or chunks_kwargs must be provided
+
+        Returns
+        -------
+        chunked : xarray.DataTree
+
+        See Also
+        --------
+        Dataset.chunk
+        Dataset.chunksizes
+        xarray.unify_chunks
+        dask.array.from_array
+        """
+        return DataTree.from_dict(
+            {
+                path: node.dataset.chunk(
+                    chunks,
+                    name_prefix=name_prefix,
+                    token=token,
+                    lock=lock,
+                    inline_array=inline_array,
+                    chunked_array_type=chunked_array_type,
+                    from_array_kwargs=from_array_kwargs,
+                    **chunks_kwargs,
+                )
+                for path, node in self.subtree_with_keys
+            },
+            name=self.name,
+        )
diff --git a/xarray/tests/test_datatree.py b/xarray/tests/test_datatree.py
index 43a45389b17..e17f7ecee25 100644
--- a/xarray/tests/test_datatree.py
+++ b/xarray/tests/test_datatree.py
@@ -2274,3 +2274,26 @@ def test_compute(self):
 
         assert actual.chunksizes == expected_chunksizes, "mismatching chunksizes"
         assert tree.chunksizes == original_chunksizes, "original tree was modified"
+
+    def test_chunk(self):
+        ds1 = xr.Dataset({"a": ("x", np.arange(10))})
+        ds2 = xr.Dataset({"b": ("y", np.arange(5))})
+        ds3 = xr.Dataset({"c": ("z", np.arange(4))})
+        ds4 = xr.Dataset({"d": ("x", np.arange(-5, 5))})
+
+        expected = xr.DataTree.from_dict(
+            {
+                "/": ds1.chunk({"x": 5}),
+                "/group1": ds2.chunk({"y": 3}),
+                "/group2": ds3.chunk({"z": 2}),
+                "/group1/subgroup1": ds4.chunk({"x": 5}),
+            }
+        )
+
+        tree = xr.DataTree.from_dict(
+            {"/": ds1, "/group1": ds2, "/group2": ds3, "/group1/subgroup1": ds4}
+        )
+        actual = tree.chunk({"x": 5, "y": 3, "z": 2})
+
+        assert_identical(actual, expected)
+        assert actual.chunksizes == expected.chunksizes