Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

Sign up for GitHub

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Jump to bottom

Multiple fastq files per pool #1

Open
boxiangliu opened this issue May 4, 2022 · 2 comments
Open

Multiple fastq files per pool #1

boxiangliu opened this issue May 4, 2022 · 2 comments

Comments

@boxiangliu
Copy link

boxiangliu commented May 4, 2022 •
edited
Loading

Dear Joseph, Jose, and colleagues,

The RNAseq data from GEO has multiple FASTQ files per pool. Each FASTQ file is around 14G (unzipped). Is it because of the SRA file size limit that you needed to split one FASTQ file per pool into multiple smaller ones?

When mapping with STAR, should I merge all FASTQ files within each pool?

Thank you,
Boxiang
@boxiangliu
Copy link
Author

Just following up with this question. @joseah

@JianingYao
Copy link

I have the same question with Boxiang. Also, did you provide code for demultiplex steps using Demuxlet like what you stated in the paper?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Assignees
No one assigned
Labels
None yet
Projects
None yet
Milestone
No milestone
Development

No branches or pull requests
2 participants
@boxiangliu @JianingYao