Visual ANalysis of timE SerieS dAta - Drosophila Activity Monitors (VANESSA-DAM) is a collection of useful tools to visualize and analyze Time series data obtained from Drosophila Activity Monitors (https://www.trikinetics.com/). The first in the series of tools is a shiny app for sleep analysis and visualization - VANESSA-DAM for sleep analysis (VANESSA-DAM-SA). For any suggestions, questions, troubleshooting or customization, please contact arijitghosh2009@gmail.com.
VANESSA-DAM-SA is dependent on Quentin Geissmann's rethomics family of packages - behavr, damr, ggetho, sleepr, for some analysis and visualization options. It offers several advantages over existing tools for sleep analysis from DAM systems, some mentionable ones are -
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Analysis and visualization of multiple monitor files, genotypes, replicates together in a high-throughput manner.
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Creating metadata files for information about experiment and better reproducibility.
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Producing high-resolution publication-quality figures with a plethora of customization.
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Data curation - automatic user-defined parameter based deletion of dead and arrhythmic individuals.
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Sleep profile analysis, various sleep parameter estimation and quantification, bout analysis, latency analysis.
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Visual comparison among genotypes, replicates.
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Dynamic plot resizing and recoloring.
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Reproducible code report so that you can generate the figures and analysis without the shiny app from RStudio directly.
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Minimizing human errors – no need to tinker with raw data to accommodate analysis tools.
A short tutorial is provided (Easy tutorial to start using VANESSA-DAM-SA.pdf) which is self explanatory and should help beginners start using the app right away.