diff --git a/CHANGELOG.md b/CHANGELOG.md
index d4d996caeb..d1a113a91e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -11,10 +11,13 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 - `opentelemetry-instrumentation-kafka-python` Instrument temporary fork, kafka-python-ng
   inside kafka-python's instrumentation
-  ([#2537](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/2537)))
+  ([#2537](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/2537))
 
 ## Breaking changes
 
+- `opentelemetry-bootstrap` Remove `opentelemetry-instrumentation-aws-lambda` from the defaults instrumentations
+  ([#2786](https://github.com/open-telemetry/opentelemetry-python-contrib/pull/2786))
+
 ## Fixed
 
 - `opentelemetry-instrumentation-fastapi` fix `fastapi` auto-instrumentation by removing `fastapi-slim` support, `fastapi-slim` itself is discontinued from maintainers
diff --git a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
index a701dbc5f4..c31863bd31 100644
--- a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
+++ b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
@@ -187,7 +187,6 @@
 ]
 default_instrumentations = [
     "opentelemetry-instrumentation-asyncio==0.48b0.dev",
-    "opentelemetry-instrumentation-aws-lambda==0.48b0.dev",
     "opentelemetry-instrumentation-dbapi==0.48b0.dev",
     "opentelemetry-instrumentation-logging==0.48b0.dev",
     "opentelemetry-instrumentation-sqlite3==0.48b0.dev",
diff --git a/scripts/generate_instrumentation_bootstrap.py b/scripts/generate_instrumentation_bootstrap.py
index 1c0cc30f7b..57af33e303 100755
--- a/scripts/generate_instrumentation_bootstrap.py
+++ b/scripts/generate_instrumentation_bootstrap.py
@@ -53,12 +53,21 @@
     "bootstrap_gen.py",
 )
 
+# AWS Lambda instrumentation is excluded from the default list because it often
+# requires specific configurations and dependencies that may not be set up
+# in all environments. Instead, users who need AWS Lambda support can opt-in
+# by manually adding it to their environment.
+# See https://github.com/open-telemetry/opentelemetry-python-contrib/issues/2787
+packages_to_exclude = ["opentelemetry-instrumentation-aws-lambda"]
+
 
 def main():
     # pylint: disable=no-member
     default_instrumentations = ast.List(elts=[])
     libraries = ast.List(elts=[])
     for pkg in get_instrumentation_packages():
+        if pkg.get("name") in packages_to_exclude:
+            continue
         if not pkg["instruments"]:
             default_instrumentations.elts.append(ast.Str(pkg["requirement"]))
         for target_pkg in pkg["instruments"]: