diff --git a/instrumentation/opentelemetry-instrumentation-urllib3/README.rst b/instrumentation/opentelemetry-instrumentation-urllib3/README.rst
index 0c53c299a0..4e0089e21e 100644
--- a/instrumentation/opentelemetry-instrumentation-urllib3/README.rst
+++ b/instrumentation/opentelemetry-instrumentation-urllib3/README.rst
@@ -38,8 +38,8 @@ The hooks can be configured as follows:
     def response_hook(span, request, response):
         pass
 
-    URLLib3Instrumentor.instrument(
-        request_hook=request_hook, response_hook=response_hook)
+    URLLib3Instrumentor().instrument(
+        request_hook=request_hook, response_hook=response_hook
     )
 
 Exclude lists
diff --git a/instrumentation/opentelemetry-instrumentation-urllib3/src/opentelemetry/instrumentation/urllib3/__init__.py b/instrumentation/opentelemetry-instrumentation-urllib3/src/opentelemetry/instrumentation/urllib3/__init__.py
index 809f9b595f..d3016ea5ee 100644
--- a/instrumentation/opentelemetry-instrumentation-urllib3/src/opentelemetry/instrumentation/urllib3/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-urllib3/src/opentelemetry/instrumentation/urllib3/__init__.py
@@ -56,8 +56,8 @@ def request_hook(span, request):
     def response_hook(span, request, response):
         pass
 
-    URLLib3Instrumentor.instrument(
-        request_hook=request_hook, response_hook=response_hook)
+    URLLib3Instrumentor().instrument(
+        request_hook=request_hook, response_hook=response_hook
     )
 
 Exclude lists