diff --git a/.github/workflows/integration.yml b/.github/workflows/integration.yml
index 1f56beff642..f82872602d2 100644
--- a/.github/workflows/integration.yml
+++ b/.github/workflows/integration.yml
@@ -16,7 +16,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        version: [netcoreapp3.1,net5.0,net6.0]
+        version: [netcoreapp3.1,net6.0]
     steps:
       - uses: actions/checkout@v3
 
@@ -28,7 +28,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        version: [netcoreapp3.1,net5.0,net6.0]
+        version: [netcoreapp3.1,net6.0]
     steps:
       - uses: actions/checkout@v3
 
@@ -40,7 +40,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        version: [netcoreapp3.1,net5.0,net6.0]
+        version: [netcoreapp3.1,net6.0]
     steps:
       - uses: actions/checkout@v3
 
@@ -52,7 +52,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        version: [netcoreapp3.1,net5.0,net6.0]
+        version: [netcoreapp3.1,net6.0]
     steps:
       - uses: actions/checkout@v3
 
diff --git a/.github/workflows/linux-ci.yml b/.github/workflows/linux-ci.yml
index e2927ea5710..2768651bb2e 100644
--- a/.github/workflows/linux-ci.yml
+++ b/.github/workflows/linux-ci.yml
@@ -16,7 +16,7 @@ jobs:
 
     strategy:
       matrix:
-        version: [netcoreapp3.1,net5.0,net6.0]
+        version: [netcoreapp3.1,net6.0]
 
     steps:
     - uses: actions/checkout@v3
diff --git a/.github/workflows/windows-ci.yml b/.github/workflows/windows-ci.yml
index 9a630b7122c..7427cc2dd2b 100644
--- a/.github/workflows/windows-ci.yml
+++ b/.github/workflows/windows-ci.yml
@@ -16,7 +16,7 @@ jobs:
 
     strategy:
       matrix:
-        version: [net462,netcoreapp3.1,net5.0,net6.0]
+        version: [net462,netcoreapp3.1,net6.0]
 
     steps:
     - uses: actions/checkout@v3
diff --git a/docs/Directory.Build.props b/docs/Directory.Build.props
index 3b32735d1c2..66de939828d 100644
--- a/docs/Directory.Build.props
+++ b/docs/Directory.Build.props
@@ -5,7 +5,7 @@
     <OutputType>Exe</OutputType>
     <!-- https://dotnet.microsoft.com/download/dotnet-core -->
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
     <!-- https://dotnet.microsoft.com/download/dotnet-framework -->
     <TargetFrameworks Condition="$(OS) == 'Windows_NT'">$(TargetFrameworks);net462;net47;net471;net472;net48</TargetFrameworks>
   </PropertyGroup>
diff --git a/src/OpenTelemetry.Exporter.ZPages/CHANGELOG.md b/src/OpenTelemetry.Exporter.ZPages/CHANGELOG.md
index 33546286c80..b0c8dc1b431 100644
--- a/src/OpenTelemetry.Exporter.ZPages/CHANGELOG.md
+++ b/src/OpenTelemetry.Exporter.ZPages/CHANGELOG.md
@@ -2,6 +2,9 @@
 
 ## Unreleased
 
+* Removes .NET Framework 4.6.1. The minimum .NET Framework
+  version supported is .NET 4.6.2. ([#3190](https://github.com/open-telemetry/opentelemetry-dotnet/issues/3190))
+
 ## 1.0.0-rc9.3
 
 Released 2022-Apr-15
diff --git a/test/Benchmarks/Benchmarks.csproj b/test/Benchmarks/Benchmarks.csproj
index 683b5ef43ae..12e759d9f1c 100644
--- a/test/Benchmarks/Benchmarks.csproj
+++ b/test/Benchmarks/Benchmarks.csproj
@@ -3,7 +3,7 @@
   <PropertyGroup>
     <OutputType>Exe</OutputType>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1;net462</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1;net462</TargetFrameworks>
     <IsPackable>false</IsPackable>
   </PropertyGroup>
 
diff --git a/test/OpenTelemetry.Exporter.Prometheus.Tests/OpenTelemetry.Exporter.Prometheus.Tests.csproj b/test/OpenTelemetry.Exporter.Prometheus.Tests/OpenTelemetry.Exporter.Prometheus.Tests.csproj
index a3f4f8a02f1..cbed29404a7 100644
--- a/test/OpenTelemetry.Exporter.Prometheus.Tests/OpenTelemetry.Exporter.Prometheus.Tests.csproj
+++ b/test/OpenTelemetry.Exporter.Prometheus.Tests/OpenTelemetry.Exporter.Prometheus.Tests.csproj
@@ -2,7 +2,7 @@
   <PropertyGroup>
     <Description>Unit test project for Prometheus Exporter for OpenTelemetry</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(OS) == 'Windows_NT'">$(TargetFrameworks);net462</TargetFrameworks>
 
     <IsPackable>false</IsPackable>
diff --git a/test/OpenTelemetry.Exporter.ZPages.Tests/OpenTelemetry.Exporter.ZPages.Tests.csproj b/test/OpenTelemetry.Exporter.ZPages.Tests/OpenTelemetry.Exporter.ZPages.Tests.csproj
index b7a16963ac7..f4157bf0599 100644
--- a/test/OpenTelemetry.Exporter.ZPages.Tests/OpenTelemetry.Exporter.ZPages.Tests.csproj
+++ b/test/OpenTelemetry.Exporter.ZPages.Tests/OpenTelemetry.Exporter.ZPages.Tests.csproj
@@ -1,8 +1,8 @@
-<Project Sdk="Microsoft.NET.Sdk">
+<Project Sdk="Microsoft.NET.Sdk">
 
   <PropertyGroup>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(OS) == 'Windows_NT'">$(TargetFrameworks);net461</TargetFrameworks>
     <IsPackable>false</IsPackable>
   </PropertyGroup>
diff --git a/test/OpenTelemetry.Extensions.Hosting.Tests/OpenTelemetry.Extensions.Hosting.Tests.csproj b/test/OpenTelemetry.Extensions.Hosting.Tests/OpenTelemetry.Extensions.Hosting.Tests.csproj
index 94e9444da31..95b8de049a2 100644
--- a/test/OpenTelemetry.Extensions.Hosting.Tests/OpenTelemetry.Extensions.Hosting.Tests.csproj
+++ b/test/OpenTelemetry.Extensions.Hosting.Tests/OpenTelemetry.Extensions.Hosting.Tests.csproj
@@ -2,7 +2,7 @@
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry .NET Core hosting library</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(OS) == 'Windows_NT'">$(TargetFrameworks);net462</TargetFrameworks>
   </PropertyGroup>
 
diff --git a/test/OpenTelemetry.Instrumentation.Http.Tests/OpenTelemetry.Instrumentation.Http.Tests.csproj b/test/OpenTelemetry.Instrumentation.Http.Tests/OpenTelemetry.Instrumentation.Http.Tests.csproj
index 5a631bb5a53..a38f1a99cba 100644
--- a/test/OpenTelemetry.Instrumentation.Http.Tests/OpenTelemetry.Instrumentation.Http.Tests.csproj
+++ b/test/OpenTelemetry.Instrumentation.Http.Tests/OpenTelemetry.Instrumentation.Http.Tests.csproj
@@ -2,7 +2,7 @@
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry HTTP instrumentations</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(OS) == 'Windows_NT'">$(TargetFrameworks);net462</TargetFrameworks>
   </PropertyGroup>
 
diff --git a/test/OpenTelemetry.Instrumentation.SqlClient.Tests/OpenTelemetry.Instrumentation.SqlClient.Tests.csproj b/test/OpenTelemetry.Instrumentation.SqlClient.Tests/OpenTelemetry.Instrumentation.SqlClient.Tests.csproj
index d5294f74346..d38abed9956 100644
--- a/test/OpenTelemetry.Instrumentation.SqlClient.Tests/OpenTelemetry.Instrumentation.SqlClient.Tests.csproj
+++ b/test/OpenTelemetry.Instrumentation.SqlClient.Tests/OpenTelemetry.Instrumentation.SqlClient.Tests.csproj
@@ -2,7 +2,7 @@
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry SqlClient instrumentations</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == ''">net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == ''">net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == '' and $(OS) == 'Windows_NT'">$(TargetFrameworks);net462</TargetFrameworks>
     <TargetFrameworks Condition="$(TARGET_FRAMEWORK) != ''">$(TARGET_FRAMEWORK)</TargetFrameworks>
   </PropertyGroup>
diff --git a/test/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests.csproj b/test/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests.csproj
index 6ac19a755de..4d80417bab2 100644
--- a/test/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests.csproj
+++ b/test/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests/OpenTelemetry.Instrumentation.StackExchangeRedis.Tests.csproj
@@ -2,7 +2,7 @@
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry StackExchangeRedis instrumentation</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == ''">net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == ''">net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == '' and $(OS) == 'Windows_NT'">$(TargetFrameworks);net462</TargetFrameworks>
     <TargetFrameworks Condition="$(TARGET_FRAMEWORK) != ''">$(TARGET_FRAMEWORK)</TargetFrameworks>
   </PropertyGroup>
diff --git a/test/OpenTelemetry.Instrumentation.W3cTraceContext.Tests/OpenTelemetry.Instrumentation.W3cTraceContext.Tests.csproj b/test/OpenTelemetry.Instrumentation.W3cTraceContext.Tests/OpenTelemetry.Instrumentation.W3cTraceContext.Tests.csproj
index fad7ce3bf04..9999ea27164 100644
--- a/test/OpenTelemetry.Instrumentation.W3cTraceContext.Tests/OpenTelemetry.Instrumentation.W3cTraceContext.Tests.csproj
+++ b/test/OpenTelemetry.Instrumentation.W3cTraceContext.Tests/OpenTelemetry.Instrumentation.W3cTraceContext.Tests.csproj
@@ -1,9 +1,9 @@
-<Project Sdk="Microsoft.NET.Sdk">
+<Project Sdk="Microsoft.NET.Sdk">
 
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry ASP.NET Core instrumentation for W3C Trace Context Trace</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == ''">net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks Condition="$(TARGET_FRAMEWORK) == ''">net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(TARGET_FRAMEWORK) != ''">$(TARGET_FRAMEWORK)</TargetFrameworks>
   </PropertyGroup>
 
@@ -33,10 +33,6 @@
     <ProjectReference Include="$(RepoRoot)\test\TestApp.AspNetCore.6.0\TestApp.AspNetCore.6.0.csproj" />
   </ItemGroup>
 
-  <ItemGroup Condition="'$(TargetFramework)' == 'net5.0'">
-    <ProjectReference Include="$(RepoRoot)\test\TestApp.AspNetCore.5.0\TestApp.AspNetCore.5.0.csproj" />
-  </ItemGroup>
-
   <ItemGroup Condition="'$(TargetFramework)' == 'netcoreapp3.1'">
     <ProjectReference Include="$(RepoRoot)\test\TestApp.AspNetCore.3.1\TestApp.AspNetCore.3.1.csproj" />
   </ItemGroup>
diff --git a/test/OpenTelemetry.Shims.OpenTracing.Tests/OpenTelemetry.Shims.OpenTracing.Tests.csproj b/test/OpenTelemetry.Shims.OpenTracing.Tests/OpenTelemetry.Shims.OpenTracing.Tests.csproj
index 0ccecfbaffe..478fc22c730 100644
--- a/test/OpenTelemetry.Shims.OpenTracing.Tests/OpenTelemetry.Shims.OpenTracing.Tests.csproj
+++ b/test/OpenTelemetry.Shims.OpenTracing.Tests/OpenTelemetry.Shims.OpenTracing.Tests.csproj
@@ -1,8 +1,8 @@
-<Project Sdk="Microsoft.NET.Sdk">
+<Project Sdk="Microsoft.NET.Sdk">
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry.Shims.OpenTracing</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
   </PropertyGroup>
 
   <ItemGroup>
diff --git a/test/OpenTelemetry.Tests.Stress.Metrics/OpenTelemetry.Tests.Stress.Metrics.csproj b/test/OpenTelemetry.Tests.Stress.Metrics/OpenTelemetry.Tests.Stress.Metrics.csproj
index 8171033131f..97d1f438292 100644
--- a/test/OpenTelemetry.Tests.Stress.Metrics/OpenTelemetry.Tests.Stress.Metrics.csproj
+++ b/test/OpenTelemetry.Tests.Stress.Metrics/OpenTelemetry.Tests.Stress.Metrics.csproj
@@ -3,7 +3,7 @@
   <PropertyGroup>
     <OutputType>Exe</OutputType>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1;net462</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1;net462</TargetFrameworks>
     <IsPackable>false</IsPackable>
   </PropertyGroup>
 
diff --git a/test/OpenTelemetry.Tests.Stress/OpenTelemetry.Tests.Stress.csproj b/test/OpenTelemetry.Tests.Stress/OpenTelemetry.Tests.Stress.csproj
index e1725d4d80c..31166433f8c 100644
--- a/test/OpenTelemetry.Tests.Stress/OpenTelemetry.Tests.Stress.csproj
+++ b/test/OpenTelemetry.Tests.Stress/OpenTelemetry.Tests.Stress.csproj
@@ -1,8 +1,8 @@
-<Project Sdk="Microsoft.NET.Sdk">
+<Project Sdk="Microsoft.NET.Sdk">
   <PropertyGroup>
     <OutputType>Exe</OutputType>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1;net462</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1;net462</TargetFrameworks>
     <IsPackable>false</IsPackable>
   </PropertyGroup>
 
diff --git a/test/OpenTelemetry.Tests/OpenTelemetry.Tests.csproj b/test/OpenTelemetry.Tests/OpenTelemetry.Tests.csproj
index 531466ed928..8d1c69f4f50 100644
--- a/test/OpenTelemetry.Tests/OpenTelemetry.Tests.csproj
+++ b/test/OpenTelemetry.Tests/OpenTelemetry.Tests.csproj
@@ -2,7 +2,7 @@
   <PropertyGroup>
     <Description>Unit test project for OpenTelemetry</Description>
     <!-- OmniSharp/VS Code requires TargetFrameworks to be in descending order for IntelliSense and analysis. -->
-    <TargetFrameworks>net6.0;net5.0;netcoreapp3.1</TargetFrameworks>
+    <TargetFrameworks>net6.0;netcoreapp3.1</TargetFrameworks>
     <TargetFrameworks Condition="$(OS) == 'Windows_NT'">$(TargetFrameworks);net462</TargetFrameworks>
     <NoWarn>$(NoWarn),CS0618</NoWarn>
   </PropertyGroup>