From c69a9a9b4511bbde9e9e7765167a4cdc8e40d14a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 16:24:19 +0100 Subject: [PATCH 001/344] Add .gitmodules file --- .../{{cookiecutter.name_noslash}}/.gitmodules | 3 +++ 1 file changed, 3 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules new file mode 100644 index 0000000000..0759cd3548 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules @@ -0,0 +1,3 @@ +[submodule "modules"] + path = modules + url = https://github.com/nf-core/modules.git From 1eeaee0857474eba8a856edf0507529317d8ea8b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 16:24:30 +0100 Subject: [PATCH 002/344] Add dsl2.preview --- nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 715d15aaea..a8fd7cf2ae 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -9,6 +9,8 @@ ---------------------------------------------------------------------------------------- */ +nextflow.preview.dsl = 2 + def helpMessage() { // TODO nf-core: Add to this help message with new command line parameters log.info nfcoreHeader() From dea2ddc47d1c13770fc053b8853db48bcdeef6f6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 16:27:55 +0100 Subject: [PATCH 003/344] Adjust spacing --- .../{{cookiecutter.name_noslash}}/.gitmodules | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules index 0759cd3548..7f0d6e3e9a 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules @@ -1,3 +1,3 @@ [submodule "modules"] - path = modules - url = https://github.com/nf-core/modules.git + path = modules + url = https://github.com/nf-core/modules.git From 33384d8748cd1e06607bb618de9a6790f9d1d041 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 16:43:42 +0100 Subject: [PATCH 004/344] Change path --- .../{{cookiecutter.name_noslash}}/.gitmodules | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules index 7f0d6e3e9a..efb4fead96 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules @@ -1,3 +1,3 @@ -[submodule "modules"] - path = modules +[submodule "nf-core"] + path = modules/nf-core url = https://github.com/nf-core/modules.git From 4e1ee0371cbd1e4cae0cf0a34e10433ae83db591 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 17:00:21 +0100 Subject: [PATCH 005/344] Remove gitmodules --- .../{{cookiecutter.name_noslash}}/.gitmodules | 3 --- 1 file changed, 3 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules deleted file mode 100644 index efb4fead96..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.gitmodules +++ /dev/null @@ -1,3 +0,0 @@ -[submodule "nf-core"] - path = modules/nf-core - url = https://github.com/nf-core/modules.git From 0856b957eef6ba31613ee9230724016194d361e7 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 18:00:37 +0100 Subject: [PATCH 006/344] Strip out boilerplate code --- .../{{cookiecutter.name_noslash}}/main.nf | 411 ++---------------- 1 file changed, 40 insertions(+), 371 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index a8fd7cf2ae..ce878ce7a8 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -11,89 +11,18 @@ nextflow.preview.dsl = 2 -def helpMessage() { - // TODO nf-core: Add to this help message with new command line parameters - log.info nfcoreHeader() - log.info""" - - Usage: - - The typical command for running the pipeline is as follows: - - nextflow run {{ cookiecutter.name }} --input '*_R{1,2}.fastq.gz' -profile docker - - Mandatory arguments: - --input [file] Path to input data (must be surrounded with quotes) - -profile [str] Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, test, awsbatch, and more - - Options: - --genome [str] Name of iGenomes reference - --single_end [bool] Specifies that the input is single-end reads - - References If not specified in the configuration file or you wish to overwrite any of the references - --fasta [file] Path to fasta reference - - Other options: - --outdir [file] The output directory where the results will be saved - --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) - --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful - --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic - - AWSBatch options: - --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch - --awsregion [str] The AWS Region for your AWS Batch job to run on - --awscli [str] Path to the AWS CLI tool - """.stripIndent() -} - -// Show help message +/* + * Print help message if required + */ +include 'modules/nf-core/functions' params(params) if (params.help) { - helpMessage() + print_help() exit 0 } /* - * SET UP CONFIGURATION VARIABLES + * Stage and check config files */ - -// Check if genome exists in the config file -if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" -} - -// TODO nf-core: Add any reference files that are needed -// Configurable reference genomes -// -// NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY -// If you want to use the channel below in a process, define the following: -// input: -// file fasta from ch_fasta -// -params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false -if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } - -// Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name -custom_runName = params.name -if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - custom_runName = workflow.runName -} - -// Check AWS batch settings -if (workflow.profile.contains('awsbatch')) { - // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." -} - -// Stage config files ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) @@ -123,315 +52,55 @@ if (params.input_paths) { .into { ch_read_files_fastqc; ch_read_files_trimming } } -// Header log info -log.info nfcoreHeader() -def summary = [:] -if (workflow.revision) summary['Pipeline Release'] = workflow.revision -summary['Run Name'] = custom_runName ?: workflow.runName -// TODO nf-core: Report custom parameters here -summary['Reads'] = params.input -summary['Fasta Ref'] = params.fasta -summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' -summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" -if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" -summary['Output dir'] = params.outdir -summary['Launch dir'] = workflow.launchDir -summary['Working dir'] = workflow.workDir -summary['Script dir'] = workflow.projectDir -summary['User'] = workflow.userName -if (workflow.profile.contains('awsbatch')) { - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue - summary['AWS CLI'] = params.awscli -} -summary['Config Profile'] = workflow.profile -if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description -if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact -if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url -summary['Config Files'] = workflow.configFiles.join(', ') -if (params.email || params.email_on_fail) { - summary['E-mail Address'] = params.email - summary['E-mail on failure'] = params.email_on_fail - summary['MultiQC maxsize'] = params.max_multiqc_email_size -} -log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") -log.info "-\033[2m--------------------------------------------------\033[0m-" - -// Check the hostnames against configured profiles -checkHostname() - -Channel.from(summary.collect{ [it.key, it.value] }) - .map { k,v -> "
$k
${v ?: 'N/A'}
" } - .reduce { a, b -> return [a, b].join("\n ") } - .map { x -> """ - id: '{{ cookiecutter.name_noslash }}-summary' - description: " - this information is collected when the pipeline is started." - section_name: '{{ cookiecutter.name }} Workflow Summary' - section_href: 'https://github.com/{{ cookiecutter.name }}' - plot_type: 'html' - data: | -
- $x -
- """.stripIndent() } - .set { ch_workflow_summary } - /* - * Parse software version numbers + * Check and print summary for parameters */ -process get_software_versions { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, - saveAs: { filename -> - if (filename.indexOf(".csv") > 0) filename - else null - } - - output: - file 'software_versions_mqc.yaml' into ch_software_versions_yaml - file "software_versions.csv" - - script: - // TODO nf-core: Get all tools to print their version number here - """ - echo $workflow.manifest.version > v_pipeline.txt - echo $workflow.nextflow.version > v_nextflow.txt - fastqc --version > v_fastqc.txt - multiqc --version > v_multiqc.txt - scrape_software_versions.py &> software_versions_mqc.yaml - """ -} +check_genome() // Check if genome exists in the config file +check_hostname() // Check the hostnames against configured profiles +check_awsbatch() // Check AWS batch settings +summary = create_summary() // Print parameter summary /* - * STEP 1 - FastQC + * FastQC */ -process fastqc { - tag "$name" - label 'process_medium' - publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, - saveAs: { filename -> - filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" - } - - input: - set val(name), file(reads) from ch_read_files_fastqc - - output: - file "*_fastqc.{zip,html}" into ch_fastqc_results - - script: - """ - fastqc --quiet --threads $task.cpus $reads - """ -} +//include 'modules/nf-core/fastqc' params(params) /* - * STEP 2 - MultiQC + * MultQC */ -process multiqc { - publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode - - input: - file (multiqc_config) from ch_multiqc_config - file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) - // TODO nf-core: Add in log files from your new processes for MultiQC to find! - file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) - file ('software_versions/*') from ch_software_versions_yaml.collect() - file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") - - output: - file "*multiqc_report.html" into ch_multiqc_report - file "*_data" - file "multiqc_plots" - - script: - rtitle = custom_runName ? "--title \"$custom_runName\"" : '' - rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' - custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' - // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time - """ - multiqc -f $rtitle $rfilename $custom_config_file . - """ -} +//include 'modules/nf-core/multiqc' params(params) /* - * STEP 3 - Output Description HTML + * Get software versions */ -process output_documentation { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode - - input: - file output_docs from ch_output_docs - file images from ch_output_docs_images +include 'modules/nf-core/get_software_versions' params(params) +get_software_versions() - output: - file "results_description.html" - - script: - """ - markdown_to_html.py $output_docs -o results_description.html - """ -} +/* + * Output markdown documentation as HTML + */ +include 'modules/nf-core/output_documentation' params(params) +output_documentation() /* - * Completion e-mail notification + * Send completion email */ workflow.onComplete { - - // Set up the e-mail variables - def subject = "[{{ cookiecutter.name }}] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[{{ cookiecutter.name }}] FAILED: $workflow.runName" - } - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = custom_runName ?: workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = ch_multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList) { - log.warn "[{{ cookiecutter.name }}] Found multiple reports from process 'multiqc', will use only one" - mqc_report = mqc_report[0] - } - } - } catch (all) { - log.warn "[{{ cookiecutter.name }}] Could not attach MultiQC report to summary email" - } - - // Check if we are only sending emails on failure - email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$baseDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$baseDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$baseDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (sendmail)" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (mail)" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_red = params.monochrome_logs ? '' : "\033[0;31m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - - if (workflow.stats.ignoredCount > 0 && workflow.success) { - log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" - log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" - log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" - } - - if (workflow.success) { - log.info "-${c_purple}[{{ cookiecutter.name }}]${c_green} Pipeline completed successfully${c_reset}-" - } else { - checkHostname() - log.info "-${c_purple}[{{ cookiecutter.name }}]${c_red} Pipeline completed with errors${c_reset}-" - } - -} - - -def nfcoreHeader() { - // Log colors ANSI codes - c_black = params.monochrome_logs ? '' : "\033[0;30m"; - c_blue = params.monochrome_logs ? '' : "\033[0;34m"; - c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; - c_dim = params.monochrome_logs ? '' : "\033[2m"; - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_white = params.monochrome_logs ? '' : "\033[0;37m"; - c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; - - return """ -${c_dim}--------------------------------------------------${c_reset}- - ${c_green},--.${c_black}/${c_green},-.${c_reset} - ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} - ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} - ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} - ${c_green}`._,._,\'${c_reset} - ${c_purple} {{ cookiecutter.name }} v${workflow.manifest.version}${c_reset} - -${c_dim}--------------------------------------------------${c_reset}- - """.stripIndent() + send_email(summary) } -def checkHostname() { - def c_reset = params.monochrome_logs ? '' : "\033[0m" - def c_white = params.monochrome_logs ? '' : "\033[0;37m" - def c_red = params.monochrome_logs ? '' : "\033[1;91m" - def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" - if (params.hostnames) { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "====================================================\n" + - " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + - " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "============================================================" - } - } - } - } -} +// Channel.from(summary.collect{ [it.key, it.value] }) +// .map { k,v -> "
$k
${v ?: 'N/A'}
" } +// .reduce { a, b -> return [a, b].join("\n ") } +// .map { x -> """ +// id: '{{ cookiecutter.name_noslash }}-summary' +// description: " - this information is collected when the pipeline is started." +// section_name: '{{ cookiecutter.name }} Workflow Summary' +// section_href: 'https://github.com/{{ cookiecutter.name }}' +// plot_type: 'html' +// data: | +//
+// $x +//
+// """.stripIndent() } +// .set { ch_workflow_summary } From efb0b8378feba7193414d86000bcb92da42464bf Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 18:01:02 +0100 Subject: [PATCH 007/344] Add module for functions --- .../modules/nf-core/functions.nf | 292 ++++++++++++++++++ 1 file changed, 292 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/functions.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/functions.nf new file mode 100644 index 0000000000..803aafd955 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/functions.nf @@ -0,0 +1,292 @@ +// // TODO nf-core: Add any reference files that are needed +// // Configurable reference genomes +// // +// // NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY +// // If you want to use the channel below in a process, define the following: +// // input: +// // file fasta from ch_fasta +// // +// params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false +// if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } + + +// // Has the run name been specified by the user? +// // this has the bonus effect of catching both -name and --name +// custom_runName = params.name +// if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { +// custom_runName = workflow.runName +// } + +/* + * Print help + */ +def print_help() { + // TODO nf-core: Add to this help message with new command line parameters + log.info nfcore_header() + log.info""" + + Usage: + + The typical command for running the pipeline is as follows: + + nextflow run {{ cookiecutter.name }} --input '*_R{1,2}.fastq.gz' -profile docker + + Mandatory arguments: + --input [file] Path to input data (must be surrounded with quotes) + -profile [str] Configuration profile to use. Can use multiple (comma separated) + Available: conda, docker, singularity, test, awsbatch, and more + + Options: + --genome [str] Name of iGenomes reference + --single_end [bool] Specifies that the input is single-end reads + + References If not specified in the configuration file or you wish to overwrite any of the references + --fasta [file] Path to fasta reference + + Other options: + --outdir [file] The output directory where the results will be saved + --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) + --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits + --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful + --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) + -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic + + AWSBatch options: + --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch + --awsregion [str] The AWS Region for your AWS Batch job to run on + --awscli [str] Path to the AWS CLI tool + """.stripIndent() +} + +/* + * Check if genome exists in the config file + */ +def check_genome() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" + } +} + +/* + * Print parameter summary + */ +def create_summary() { + // Header log info + log.info nfcore_header() + def summary = [:] + if (workflow.revision) summary['Pipeline Release'] = workflow.revision + summary['Run Name'] = custom_runName ?: workflow.runName + // TODO nf-core: Report custom parameters here + summary['Reads'] = params.input + summary['Fasta Ref'] = params.fasta + summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' + summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" + if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" + summary['Output dir'] = params.outdir + summary['Launch dir'] = workflow.launchDir + summary['Working dir'] = workflow.workDir + summary['Script dir'] = workflow.projectDir + summary['User'] = workflow.userName + if (workflow.profile.contains('awsbatch')) { + summary['AWS Region'] = params.awsregion + summary['AWS Queue'] = params.awsqueue + summary['AWS CLI'] = params.awscli + } + summary['Config Profile'] = workflow.profile + if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description + if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact + if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url + summary['Config Files'] = workflow.configFiles.join(', ') + if (params.email || params.email_on_fail) { + summary['E-mail Address'] = params.email + summary['E-mail on failure'] = params.email_on_fail + summary['MultiQC maxsize'] = params.max_multiqc_email_size + } + log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") + log.info "-\033[2m--------------------------------------------------\033[0m-" + + return summary +} + +/* + * ANSI nf-core header + */ +def nfcore_header() { + // Log colors ANSI codes + c_black = params.monochrome_logs ? '' : "\033[0;30m"; + c_blue = params.monochrome_logs ? '' : "\033[0;34m"; + c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; + c_dim = params.monochrome_logs ? '' : "\033[2m"; + c_green = params.monochrome_logs ? '' : "\033[0;32m"; + c_purple = params.monochrome_logs ? '' : "\033[0;35m"; + c_reset = params.monochrome_logs ? '' : "\033[0m"; + c_white = params.monochrome_logs ? '' : "\033[0;37m"; + c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; + + return """ -${c_dim}--------------------------------------------------${c_reset}- + ${c_green},--.${c_black}/${c_green},-.${c_reset} + ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} + ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} + ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} + ${c_green}`._,._,\'${c_reset} + ${c_purple} {{ cookiecutter.name }} v${workflow.manifest.version}${c_reset} + -${c_dim}--------------------------------------------------${c_reset}- + """.stripIndent() +} + +/* + * Check the hostnames against configured profiles + */ +def check_hostname() { + def c_reset = params.monochrome_logs ? '' : "\033[0m" + def c_white = params.monochrome_logs ? '' : "\033[0;37m" + def c_red = params.monochrome_logs ? '' : "\033[1;91m" + def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.error "====================================================\n" + + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + + " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + + "============================================================" + } + } + } + } +} + +/* + * Check AWS batch settings + */ +def check_awsbatch() { + if (workflow.profile.contains('awsbatch')) { + // AWSBatch sanity checking + if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + // related: https://github.com/nextflow-io/nextflow/issues/813 + if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + // Prevent trace files to be stored on S3 since S3 does not support rolling files. + if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + } +} + +/* + * Completion e-mail notification + */ +def send_email(summary) { + + // Set up the e-mail variables + def subject = "[{{ cookiecutter.name }}] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[{{ cookiecutter.name }}] FAILED: $workflow.runName" + } + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = custom_runName ?: workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary + email_fields['summary']['Date Started'] = workflow.start + email_fields['summary']['Date Completed'] = workflow.complete + email_fields['summary']['Pipeline script file path'] = workflow.scriptFile + email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision + email_fields['summary']['Nextflow Version'] = workflow.nextflow.version + email_fields['summary']['Nextflow Build'] = workflow.nextflow.build + email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = ch_multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList) { + log.warn "[{{ cookiecutter.name }}] Found multiple reports from process 'multiqc', will use only one" + mqc_report = mqc_report[0] + } + } + } catch (all) { + log.warn "[{{ cookiecutter.name }}] Could not attach MultiQC report to summary email" + } + + // Check if we are only sending emails on failure + email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$baseDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$baseDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] + def sf = new File("$baseDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (sendmail)" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (mail)" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + + c_green = params.monochrome_logs ? '' : "\033[0;32m"; + c_purple = params.monochrome_logs ? '' : "\033[0;35m"; + c_red = params.monochrome_logs ? '' : "\033[0;31m"; + c_reset = params.monochrome_logs ? '' : "\033[0m"; + + if (workflow.stats.ignoredCount > 0 && workflow.success) { + log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" + log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" + log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" + } + + if (workflow.success) { + log.info "-${c_purple}[{{ cookiecutter.name }}]${c_green} Pipeline completed successfully${c_reset}-" + } else { + checkHostname() + log.info "-${c_purple}[{{ cookiecutter.name }}]${c_red} Pipeline completed with errors${c_reset}-" + } +} From 1d442cfcf7bc30932818bd79dc91b7a4ed4eb6f6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 18:01:25 +0100 Subject: [PATCH 008/344] Add module for get_software_versions --- .../modules/nf-core/get_software_versions.nf | 24 +++++++++++++++++++ 1 file changed, 24 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/get_software_versions.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/get_software_versions.nf new file mode 100644 index 0000000000..74f24823b4 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/get_software_versions.nf @@ -0,0 +1,24 @@ +/* + * Parse software version numbers + */ +process get_software_versions { + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, + saveAs: { filename -> + if (filename.indexOf(".csv") > 0) filename + else null + } + + output: + file 'software_versions_mqc.yaml' + file "software_versions.csv" + + script: + // TODO nf-core: Get all tools to print their version number here + """ + echo $workflow.manifest.version > v_pipeline.txt + echo $workflow.nextflow.version > v_nextflow.txt + fastqc --version > v_fastqc.txt + multiqc --version > v_multiqc.txt + scrape_software_versions.py &> software_versions_mqc.yaml + """ +} From 49161ffcab4442718416863bb063a75eb7f323fd Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 18:01:36 +0100 Subject: [PATCH 009/344] Add module for output_documentation --- .../modules/nf-core/output_documentation.nf | 18 ++++++++++++++++++ 1 file changed, 18 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/output_documentation.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/output_documentation.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/output_documentation.nf new file mode 100644 index 0000000000..37688ff65c --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/output_documentation.nf @@ -0,0 +1,18 @@ +/* + * Output description HTML + */ +process output_documentation { + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode + + input: + file output_docs from ch_output_docs + file images from ch_output_docs_images + + output: + file "results_description.html" + + script: + """ + markdown_to_html.py $output_docs -o results_description.html + """ +} From 539202e49ce072b5dbbdf171d144eb9e5c62783c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 18:02:29 +0100 Subject: [PATCH 010/344] Add module for FastQC --- .../modules/tools/fastqc.nf | 23 +++++++++++++++++++ 1 file changed, 23 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/fastqc.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/fastqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/fastqc.nf new file mode 100644 index 0000000000..463b9dcfa8 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/fastqc.nf @@ -0,0 +1,23 @@ +/* + * FastQC + */ +process fastqc { + tag "$name" + label 'process_medium' + publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, + saveAs: { filename -> + filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" + } + + input: + set val(name), file(reads) from ch_read_files_fastqc + + output: + file "*_fastqc.{zip,html}" into ch_fastqc_results + + script: + """ + fastqc --quiet --threads $task.cpus $reads + """ +} + From 5f9c03fe7ad0023260431a74abe1bdbb7c89664a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 6 Jul 2020 18:02:40 +0100 Subject: [PATCH 011/344] Add module for MultiQC --- .../modules/tools/multiqc.nf | 28 +++++++++++++++++++ 1 file changed, 28 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/multiqc.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/multiqc.nf new file mode 100644 index 0000000000..8322f82abc --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/multiqc.nf @@ -0,0 +1,28 @@ +/* + * STEP 2 - MultiQC + */ +process MULTIQC { + publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode + + input: + file (multiqc_config) from ch_multiqc_config + file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) + // TODO nf-core: Add in log files from your new processes for MultiQC to find! + file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) + file ('software_versions/*') from ch_software_versions_yaml.collect() + file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") + + output: + file "*multiqc_report.html" into ch_multiqc_report + file "*_data" + file "multiqc_plots" + + script: + rtitle = custom_runName ? "--title \"$custom_runName\"" : '' + rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' + custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' + // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time + """ + multiqc -f $rtitle $rfilename $custom_config_file . + """ +} From 86512d529963e6068d6bbc5fa2e4798d29050e08 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 08:53:29 +0100 Subject: [PATCH 012/344] Reorganise code --- .../modules/{nf-core => local}/functions.nf | 0 .../modules/{nf-core => local}/get_software_versions.nf | 0 .../modules/{nf-core => local}/output_documentation.nf | 0 .../modules/{tools => nf-core}/fastqc.nf | 0 .../modules/{tools => nf-core}/multiqc.nf | 0 5 files changed, 0 insertions(+), 0 deletions(-) rename nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/{nf-core => local}/functions.nf (100%) rename nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/{nf-core => local}/get_software_versions.nf (100%) rename nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/{nf-core => local}/output_documentation.nf (100%) rename nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/{tools => nf-core}/fastqc.nf (100%) rename nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/{tools => nf-core}/multiqc.nf (100%) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf similarity index 100% rename from nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/functions.nf rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf similarity index 100% rename from nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/get_software_versions.nf rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/output_documentation.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf similarity index 100% rename from nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/output_documentation.nf rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/fastqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf similarity index 100% rename from nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/fastqc.nf rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf similarity index 100% rename from nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/tools/multiqc.nf rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf From 3917afebb7d00068913a7f07e8b80ea34174a2ab Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 09:13:25 +0100 Subject: [PATCH 013/344] Add placeholder files for Groovy code --- .gitignore | 1 - .../{{cookiecutter.name_noslash}}/lib/checks.groovy | 0 .../{{cookiecutter.name_noslash}}/lib/headers.groovy | 0 .../{{cookiecutter.name_noslash}}/lib/params.groovy | 0 4 files changed, 1 deletion(-) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy diff --git a/.gitignore b/.gitignore index 27c031f40c..4c7622c9ff 100644 --- a/.gitignore +++ b/.gitignore @@ -20,7 +20,6 @@ dist/ downloads/ eggs/ .eggs/ -lib/ lib64/ parts/ sdist/ diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy new file mode 100644 index 0000000000..e69de29bb2 diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy new file mode 100644 index 0000000000..e69de29bb2 diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy new file mode 100644 index 0000000000..e69de29bb2 From 41807a1e005d80d51051f39d53fe1fac8cc5f2f0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 09:30:29 +0100 Subject: [PATCH 014/344] Add brief description --- .../{{cookiecutter.name_noslash}}/lib/checks.groovy | 2 ++ .../{{cookiecutter.name_noslash}}/lib/headers.groovy | 2 ++ .../{{cookiecutter.name_noslash}}/lib/params.groovy | 2 ++ 3 files changed, 6 insertions(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy index e69de29bb2..2e3050433e 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy @@ -0,0 +1,2 @@ +/** This file holds several functions used to perform standard checks in the nf-core pipeline template e.g. hostname, AWS batch and genome checks. +**/ diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy index e69de29bb2..a90ede8c41 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy @@ -0,0 +1,2 @@ +/** This file holds several functions used to render the nf-core ANSI header. +**/ diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy index e69de29bb2..fe1880dd07 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy @@ -0,0 +1,2 @@ +/** This file holds several functions used to perform JSON parameter validation, help and summary rendering in the nf-core pipeline template. +**/ From 89efb7278de2d7d33a0cdba3c0b1b4fedf82cbb2 Mon Sep 17 00:00:00 2001 From: Alexander Peltzer Date: Tue, 7 Jul 2020 10:43:59 +0200 Subject: [PATCH 015/344] Add in Groovy Library --- lib/CommonFunctions.groovy | 247 +++++++++++++++++++++++++++++++++++++ 1 file changed, 247 insertions(+) create mode 100644 lib/CommonFunctions.groovy diff --git a/lib/CommonFunctions.groovy b/lib/CommonFunctions.groovy new file mode 100644 index 0000000000..f187989159 --- /dev/null +++ b/lib/CommonFunctions.groovy @@ -0,0 +1,247 @@ +/** This file holds several functions that can be used across many nf-core pipelines arbitrarily without having the main code in the pipelines specific repository in the future. Based on work by Patrick Huether (@phue), some updates and cleanup by (@apeltzer). +**/ + +@Grab('ch.qos.logback:logback-classic:1.2.3') + +import groovy.transform.CompileStatic +import groovy.json.JsonSlurper + +@groovy.util.logging.Slf4j +//@CompileStatic +class CommonFunctions { + + private static Map generateLogColors(Boolean monochromeLogs) { + Map colorcodes = [:] + colorcodes['reset'] = monochromeLogs ? '' : "\033[0m" + colorcodes['dim'] = monochromeLogs ? '' : "\033[2m" + colorcodes['black'] = monochromeLogs ? '' : "\033[0;30m" + colorcodes['green'] = monochromeLogs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochromeLogs ? '' : "\033[0;33m" + colorcodes['yellow_bold'] = monochromeLogs ? '' : "\033[1;93m" + colorcodes['blue'] = monochromeLogs ? '' : "\033[0;34m" + colorcodes['purple'] = monochromeLogs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochromeLogs ? '' : "\033[0;36m" + colorcodes['white'] = monochromeLogs ? '' : "\033[0;37m" + colorcodes['red'] = monochromeLogs ? '' : "\033[1;91m" + return colorcodes + } + + /* + * This method tries to read the params file from JSON settings file given a path to a schema. + * Can be directly used in the main.nf file to read in JSON schema and have a LinkedHashMap then with contents + e.g. def paramsWithUsage = CommonFunctions.readParamsFromJsonSettings("$baseDir/nextflow_schema.json") + */ + private static LinkedHashMap readParamsFromJsonSettings(String path) { + def paramsWithUsage = new LinkedHashMap() + try { + paramsWithUsage = tryReadParamsFromJsonSettings(path) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + paramsWithUsage = new LinkedHashMap() + } + return paramsWithUsage + } + + /* + Method to actually read in JSON file using Groovy. + Group (as Key), values are all parameters + - Parameter1 as Key, Description as Value + - Parameter2 as Key, Description as Value + .... + Group + - + */ + private static LinkedHashMap tryReadParamsFromJsonSettings(String path) throws Exception { + + def paramsContent = new File(path).text + def Map paramsWithUsage = (Map) new JsonSlurper().parseText(paramsContent).get('properties') + + /* Tree looks like this in nf-core schema + * properties <- this is what the first get('properties') gets us + group 1 + properties + description + group 2 + properties + description + group 3 + properties + description + */ + def output_map = new LinkedHashMap() + + //Lets go deeper + paramsWithUsage.each { key, val -> + //println "Group: $key\n" //Gets the Group names + def Map submap = paramsWithUsage."$key".properties //Gets the property object of the group + //println "Parameters:\n" //nested access to the Parameters + def sub_params = new LinkedHashMap() + submap.each { innerkey, value -> + //println "$innerkey\t$value.description" + sub_params.put("$innerkey", "$value.description") + } + output_map.put("$key", sub_params) + //println "\n" + } + return output_map + } + + static String helpMessage(paramsWithUsage, workflow) { + + def usageHelp = String.format( + """\ + Usage: + The typical command for running the pipeline is as follows: + nextflow run ${workflow.manifest.name} v${workflow.manifest.version} --input 'input.tsv' -profile docker + Options: + """.stripIndent(), println(prettyFormatJSON(paramsWithUsage))) + } + + static String prettyFormatJSON(paramsWithUsage){ + //Todo make this really nicer ... this is just experimental code + String output = "" + for(group in paramsWithUsage.keySet()) { + output += "Group: " + group + "\n" + def params = paramsWithUsage.get(group) //This gets the parameters of that particular group + for(par in params.keySet()) { + output+= "\u001B[1m" + par + "\u001B[1m" + "\t" + params.get(par) + "\n" + } + output += "\n" //Extra newline after parameter block + } + + return output + } + + static String nfcoreHeader(params, workflow) { + Map colors = generateLogColors(params.monochrome_logs) + def showHeader = String.format( + """\n + ${colors.dim}----------------------------------------------------${colors.reset} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${colors.dim}----------------------------------------------------${colors.reset} + """.stripIndent()) + } + static void checkHostname(params) { + Map colors = generateLogColors(params.monochrome_logs) + if(params.hostnames){ + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if(hostname.contains(hname) && !workflow.profile.contains(prof)){ + log.error "====================================================\n" + + " ${colors.red}WARNING!${colors.reset} You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}\n" + + " ${colors.yellow_bold}It's highly recommended that you use `-profile $prof${colors.reset}`\n" + + "============================================================" + } + } + } + } + } + static void checkAWSbatch(params, workflow) { + + assert !params.awsqueue || !params.awsregion : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + // related: https://github.com/nextflow-io/nextflow/issues/813 + assert !params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + // Prevent trace files to be stored on S3 since S3 does not support rolling files. + assert workflow.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + } + static void workflowReport(summary, workflow, params, baseDir, mqc_report) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if(!workflow.success){ + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary + email_fields['summary']['Date Started'] = workflow.start + email_fields['summary']['Date Completed'] = workflow.complete + email_fields['summary']['Pipeline script file path'] = workflow.scriptFile + email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId + if(workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository + if(workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId + if(workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision + if(workflow.container) email_fields['summary']['Docker image'] = workflow.container + email_fields['summary']['Nextflow Version'] = workflow.nextflow.version + email_fields['summary']['Nextflow Build'] = workflow.nextflow.build + email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$baseDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$baseDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def smail_fields = [ email: params.email, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.maxMultiqcEmailFileSize.toBytes() ] + def sf = new File("$baseDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + if (params.email) { + try { + if( params.plaintext_email ){ throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "[$workflow.manifest.name] Sent summary e-mail to $params.email (sendmail)" + } catch (all) { + if (mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { + [ 'mail', '-s', subject, params.email, '-A', mqc_report ].execute() << email_txt + } else { + // Catch failures and try with plaintext + [ 'mail', '-s', subject, params.email ].execute() << email_txt + } + log.info "[$workflow.manifest.name] Sent summary e-mail to $params.email (mail)" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File( "${params.outdir}/pipeline_info/" ) + if( !output_d.exists() ) { + output_d.mkdirs() + } + def output_hf = new File( output_d, "pipeline_report.html" ) + output_hf.withWriter { w -> w << email_html } + def output_tf = new File( output_d, "pipeline_report.txt" ) + output_tf.withWriter { w -> w << email_txt } + + Map colors = generateLogColors(params.monochrome_logs) + + if (workflow.stats.ignoredCountFmt > 0 && workflow.success) { + log.info "${colors.purple}Warning, pipeline completed, but with errored process(es) ${colors.reset}" + log.info "${colors.red}Number of ignored errored process(es) : ${workflow.stats.ignoredCountFmt} ${colors.reset}" + log.info "${colors.green}Number of successfully ran process(es) : ${workflow.stats.succeedCountFmt} ${colors.reset}" + } + + if (workflow.success) { + log.info "${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}" + } else { + checkHostname(params) + log.info "${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}" + } + } +} + From 3bf36169be773866dd69eef5386a41d45db1c677 Mon Sep 17 00:00:00 2001 From: Alexander Peltzer Date: Tue, 7 Jul 2020 10:45:55 +0200 Subject: [PATCH 016/344] Add in CommonFunctions --- .../{{cookiecutter.name_noslash}}/lib}/CommonFunctions.groovy | 1 + 1 file changed, 1 insertion(+) rename {lib => nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib}/CommonFunctions.groovy (99%) diff --git a/lib/CommonFunctions.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy similarity index 99% rename from lib/CommonFunctions.groovy rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy index f187989159..d7ffd457e4 100644 --- a/lib/CommonFunctions.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy @@ -99,6 +99,7 @@ class CommonFunctions { static String prettyFormatJSON(paramsWithUsage){ //Todo make this really nicer ... this is just experimental code + //ASCII codes seem to work fine String output = "" for(group in paramsWithUsage.keySet()) { output += "Group: " + group + "\n" From f7a369aaf47f8a9c99f041904514e2b04185aaea Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:25:29 +0100 Subject: [PATCH 017/344] Update pipeline command --- .../pipeline-template/{{cookiecutter.name_noslash}}/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index 169587892b..efb2124aa6 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -33,7 +33,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ```bash - nextflow run {{ cookiecutter.name }} -profile --input '*_R{1,2}.fastq.gz' --genome GRCh37 + nextflow run {{ cookiecutter.name }} -profile --input samplesheet.csv --genome GRCh37 ``` See [usage docs](docs/usage.md) for all of the available options when running the pipeline. From 5e91faf78fd5eb5d54f85c695e531e5817eaa4ba Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:25:44 +0100 Subject: [PATCH 018/344] Update input parameter docs --- .../docs/usage.md | 30 +++++++++---------- 1 file changed, 15 insertions(+), 15 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/usage.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/usage.md index 53d09b2352..ab8f5d24f8 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/usage.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/usage.md @@ -10,7 +10,6 @@ * [Main arguments](#main-arguments) * [`-profile`](#-profile) * [`--input`](#--input) - * [`--single_end`](#--single_end) * [Reference genomes](#reference-genomes) * [`--genome` (using iGenomes)](#--genome-using-igenomes) * [`--fasta`](#--fasta) @@ -57,7 +56,7 @@ NXF_OPTS='-Xms1g -Xmx4g' The typical command for running the pipeline is as follows: ```bash -nextflow run {{ cookiecutter.name }} --input '*_R{1,2}.fastq.gz' -profile docker +nextflow run {{ cookiecutter.name }} --input samplesheet.csv -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -122,29 +121,30 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof ### `--input` -Use this to specify the location of your input FastQ files. For example: +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. ```bash ---input 'path/to/data/sample_*_{1,2}.fastq' +--input '[path to samplesheet file]' ``` -Please note the following requirements: +#### Format -1. The path must be enclosed in quotes -2. The path must have at least one `*` wildcard character -3. When using the pipeline with paired end data, the path must use `{1,2}` notation to specify read pairs. +The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once (e.g. to increase sequencing depth). The pipeline will perform the analysis in parallel, and subsequently merge them when required. -If left unspecified, a default pattern is used: `data/*{1,2}.fastq.gz` - -### `--single_end` - -By default, the pipeline expects paired-end data. If you have single-end data, you need to specify `--single_end` on the command line when you launch the pipeline. A normal glob pattern, enclosed in quotation marks, can then be used for `--input`. For example: +A final design file may look something like the one below. `SAMPLE_1` was sequenced twice in Illumina PE format and `SAMPLE_2` was sequenced once in Illumina SE format. ```bash ---single_end --input '*.fastq' +sample,fastq_1,fastq_2 +SAMPLE_1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +SAMPLE_1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz +SAMPLE_2,AEG588A2_S4_L003_R1_001.fastq.gz, ``` -It is not possible to run a mixture of single-end and paired-end files in one run. +| Column | Description | +|-----------|----------------------------------------------------------------------------------------------------------------------------| +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | ## Reference genomes From 7c11e623e1863f38036fc2311277542ca94a4723 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:25:51 +0100 Subject: [PATCH 019/344] Update packages --- .../{{cookiecutter.name_noslash}}/environment.yml | 15 +++++++++------ 1 file changed, 9 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml index 8950af95de..04ed0dafb2 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml @@ -6,10 +6,13 @@ channels: - bioconda - defaults dependencies: - - conda-forge::python=3.7.3 - - conda-forge::markdown=3.1.1 - - conda-forge::pymdown-extensions=6.0 - - conda-forge::pygments=2.5.2 # TODO nf-core: Add required software dependencies here - - bioconda::fastqc=0.11.8 - - bioconda::multiqc=1.7 + ## conda-forge packages + - conda-forge::python=3.8.3 + - conda-forge::markdown=3.2.2 + - conda-forge::pymdown-extensions=7.1 + - conda-forge::pygments=2.6.1 + + ## bioconda packages + - bioconda::fastqc=0.11.9 + - bioconda::multiqc=1.9 From 131eac00526e94eafb318bec22875a4c340c51b0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:26:01 +0100 Subject: [PATCH 020/344] Refine functions --- .../modules/local/functions.nf | 570 +++++++++--------- 1 file changed, 278 insertions(+), 292 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf index 803aafd955..40935217ce 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf @@ -1,292 +1,278 @@ -// // TODO nf-core: Add any reference files that are needed -// // Configurable reference genomes -// // -// // NOTE - THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY -// // If you want to use the channel below in a process, define the following: -// // input: -// // file fasta from ch_fasta -// // -// params.fasta = params.genome ? params.genomes[ params.genome ].fasta ?: false : false -// if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } - - -// // Has the run name been specified by the user? -// // this has the bonus effect of catching both -name and --name -// custom_runName = params.name -// if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { -// custom_runName = workflow.runName -// } - -/* - * Print help - */ -def print_help() { - // TODO nf-core: Add to this help message with new command line parameters - log.info nfcore_header() - log.info""" - - Usage: - - The typical command for running the pipeline is as follows: - - nextflow run {{ cookiecutter.name }} --input '*_R{1,2}.fastq.gz' -profile docker - - Mandatory arguments: - --input [file] Path to input data (must be surrounded with quotes) - -profile [str] Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, test, awsbatch, and more - - Options: - --genome [str] Name of iGenomes reference - --single_end [bool] Specifies that the input is single-end reads - - References If not specified in the configuration file or you wish to overwrite any of the references - --fasta [file] Path to fasta reference - - Other options: - --outdir [file] The output directory where the results will be saved - --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) - --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful - --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic - - AWSBatch options: - --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch - --awsregion [str] The AWS Region for your AWS Batch job to run on - --awscli [str] Path to the AWS CLI tool - """.stripIndent() -} - -/* - * Check if genome exists in the config file - */ -def check_genome() { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" - } -} - -/* - * Print parameter summary - */ -def create_summary() { - // Header log info - log.info nfcore_header() - def summary = [:] - if (workflow.revision) summary['Pipeline Release'] = workflow.revision - summary['Run Name'] = custom_runName ?: workflow.runName - // TODO nf-core: Report custom parameters here - summary['Reads'] = params.input - summary['Fasta Ref'] = params.fasta - summary['Data Type'] = params.single_end ? 'Single-End' : 'Paired-End' - summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" - if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" - summary['Output dir'] = params.outdir - summary['Launch dir'] = workflow.launchDir - summary['Working dir'] = workflow.workDir - summary['Script dir'] = workflow.projectDir - summary['User'] = workflow.userName - if (workflow.profile.contains('awsbatch')) { - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue - summary['AWS CLI'] = params.awscli - } - summary['Config Profile'] = workflow.profile - if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description - if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact - if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url - summary['Config Files'] = workflow.configFiles.join(', ') - if (params.email || params.email_on_fail) { - summary['E-mail Address'] = params.email - summary['E-mail on failure'] = params.email_on_fail - summary['MultiQC maxsize'] = params.max_multiqc_email_size - } - log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") - log.info "-\033[2m--------------------------------------------------\033[0m-" - - return summary -} - -/* - * ANSI nf-core header - */ -def nfcore_header() { - // Log colors ANSI codes - c_black = params.monochrome_logs ? '' : "\033[0;30m"; - c_blue = params.monochrome_logs ? '' : "\033[0;34m"; - c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; - c_dim = params.monochrome_logs ? '' : "\033[2m"; - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_white = params.monochrome_logs ? '' : "\033[0;37m"; - c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; - - return """ -${c_dim}--------------------------------------------------${c_reset}- - ${c_green},--.${c_black}/${c_green},-.${c_reset} - ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} - ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} - ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} - ${c_green}`._,._,\'${c_reset} - ${c_purple} {{ cookiecutter.name }} v${workflow.manifest.version}${c_reset} - -${c_dim}--------------------------------------------------${c_reset}- - """.stripIndent() -} - -/* - * Check the hostnames against configured profiles - */ -def check_hostname() { - def c_reset = params.monochrome_logs ? '' : "\033[0m" - def c_white = params.monochrome_logs ? '' : "\033[0;37m" - def c_red = params.monochrome_logs ? '' : "\033[1;91m" - def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" - if (params.hostnames) { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "====================================================\n" + - " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + - " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "============================================================" - } - } - } - } -} - -/* - * Check AWS batch settings - */ -def check_awsbatch() { - if (workflow.profile.contains('awsbatch')) { - // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." - } -} - -/* - * Completion e-mail notification - */ -def send_email(summary) { - - // Set up the e-mail variables - def subject = "[{{ cookiecutter.name }}] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[{{ cookiecutter.name }}] FAILED: $workflow.runName" - } - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = custom_runName ?: workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = ch_multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList) { - log.warn "[{{ cookiecutter.name }}] Found multiple reports from process 'multiqc', will use only one" - mqc_report = mqc_report[0] - } - } - } catch (all) { - log.warn "[{{ cookiecutter.name }}] Could not attach MultiQC report to summary email" - } - - // Check if we are only sending emails on failure - email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$baseDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$baseDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$baseDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (sendmail)" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (mail)" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_red = params.monochrome_logs ? '' : "\033[0;31m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - - if (workflow.stats.ignoredCount > 0 && workflow.success) { - log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" - log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" - log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" - } - - if (workflow.success) { - log.info "-${c_purple}[{{ cookiecutter.name }}]${c_green} Pipeline completed successfully${c_reset}-" - } else { - checkHostname() - log.info "-${c_purple}[{{ cookiecutter.name }}]${c_red} Pipeline completed with errors${c_reset}-" - } -} +// Has the run name been specified by the user? +// this has the bonus effect of catching both -name and --name +custom_runName = params.name +if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { + custom_runName = workflow.runName +} + +/* + * Print help + */ +def print_help() { + // TODO nf-core: Add to this help message with new command line parameters + log.info nfcore_header() + log.info""" + + Usage: + + The typical command for running the pipeline is as follows: + + nextflow run {{ cookiecutter.name }} --input samplesheet.csv -profile docker + + Mandatory arguments: + --input [file] Comma-separated file containing information about the samples in the experiment (see docs/usage.md) + -profile [str] Configuration profile to use. Can use multiple (comma separated) + Available: conda, docker, singularity, test, awsbatch, and more + + Options: + --genome [str] Name of iGenomes reference + + References If not specified in the configuration file or you wish to overwrite any of the references + --fasta [file] Path to fasta reference + + Other options: + --outdir [file] The output directory where the results will be saved + --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) + --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits + --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful + --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) + -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic + + AWSBatch options: + --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch + --awsregion [str] The AWS Region for your AWS Batch job to run on + --awscli [str] Path to the AWS CLI tool + """.stripIndent() +} + +/* + * Check if genome exists in the config file + */ +def check_genome() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" + } +} + +/* + * Print parameter summary + */ +def create_summary() { + // Header log info + log.info nfcore_header() + def summary = [:] + if (workflow.revision) summary['Pipeline Release'] = workflow.revision + summary['Run Name'] = custom_runName ?: workflow.runName + // TODO nf-core: Report custom parameters here + summary['Samplesheet'] = params.input + summary['Fasta File'] = params.fasta + summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" + if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" + summary['Output dir'] = params.outdir + summary['Launch dir'] = workflow.launchDir + summary['Working dir'] = workflow.workDir + summary['Script dir'] = workflow.projectDir + summary['User'] = workflow.userName + if (workflow.profile.contains('awsbatch')) { + summary['AWS Region'] = params.awsregion + summary['AWS Queue'] = params.awsqueue + summary['AWS CLI'] = params.awscli + } + summary['Config Profile'] = workflow.profile + if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description + if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact + if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url + summary['Config Files'] = workflow.configFiles.join(', ') + if (params.email || params.email_on_fail) { + summary['E-mail Address'] = params.email + summary['E-mail on failure'] = params.email_on_fail + summary['MultiQC maxsize'] = params.max_multiqc_email_size + } + log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") + log.info "-\033[2m--------------------------------------------------\033[0m-" + + return summary +} + +/* + * ANSI nf-core header + */ +def nfcore_header() { + // Log colors ANSI codes + c_black = params.monochrome_logs ? '' : "\033[0;30m"; + c_blue = params.monochrome_logs ? '' : "\033[0;34m"; + c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; + c_dim = params.monochrome_logs ? '' : "\033[2m"; + c_green = params.monochrome_logs ? '' : "\033[0;32m"; + c_purple = params.monochrome_logs ? '' : "\033[0;35m"; + c_reset = params.monochrome_logs ? '' : "\033[0m"; + c_white = params.monochrome_logs ? '' : "\033[0;37m"; + c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; + + return """ -${c_dim}--------------------------------------------------${c_reset}- + ${c_green},--.${c_black}/${c_green},-.${c_reset} + ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} + ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} + ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} + ${c_green}`._,._,\'${c_reset} + ${c_purple} {{ cookiecutter.name }} v${workflow.manifest.version}${c_reset} + -${c_dim}--------------------------------------------------${c_reset}- + """.stripIndent() +} + +/* + * Check the hostnames against configured profiles + */ +def check_hostname() { + def c_reset = params.monochrome_logs ? '' : "\033[0m" + def c_white = params.monochrome_logs ? '' : "\033[0;37m" + def c_red = params.monochrome_logs ? '' : "\033[1;91m" + def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.error "====================================================\n" + + " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + + " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + + "============================================================" + } + } + } + } +} + +/* + * Check AWS batch settings + */ +def check_awsbatch() { + if (workflow.profile.contains('awsbatch')) { + // AWSBatch sanity checking + if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + // related: https://github.com/nextflow-io/nextflow/issues/813 + if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + // Prevent trace files to be stored on S3 since S3 does not support rolling files. + if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + } +} + +/* + * Completion e-mail notification + */ +def send_email(summary) { + + // Set up the e-mail variables + def subject = "[{{ cookiecutter.name }}] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[{{ cookiecutter.name }}] FAILED: $workflow.runName" + } + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = custom_runName ?: workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary + email_fields['summary']['Date Started'] = workflow.start + email_fields['summary']['Date Completed'] = workflow.complete + email_fields['summary']['Pipeline script file path'] = workflow.scriptFile + email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision + email_fields['summary']['Nextflow Version'] = workflow.nextflow.version + email_fields['summary']['Nextflow Build'] = workflow.nextflow.build + email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = ch_multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList) { + log.warn "[{{ cookiecutter.name }}] Found multiple reports from process 'multiqc', will use only one" + mqc_report = mqc_report[0] + } + } + } catch (all) { + log.warn "[{{ cookiecutter.name }}] Could not attach MultiQC report to summary email" + } + + // Check if we are only sending emails on failure + email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$baseDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$baseDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] + def sf = new File("$baseDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (sendmail)" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (mail)" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + + c_green = params.monochrome_logs ? '' : "\033[0;32m"; + c_purple = params.monochrome_logs ? '' : "\033[0;35m"; + c_red = params.monochrome_logs ? '' : "\033[0;31m"; + c_reset = params.monochrome_logs ? '' : "\033[0m"; + + if (workflow.stats.ignoredCount > 0 && workflow.success) { + log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" + log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" + log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" + } + + if (workflow.success) { + log.info "-${c_purple}[{{ cookiecutter.name }}]${c_green} Pipeline completed successfully${c_reset}-" + } else { + checkHostname() + log.info "-${c_purple}[{{ cookiecutter.name }}]${c_red} Pipeline completed with errors${c_reset}-" + } +} From 72bffef0851596ea522e2e2471a909420e254ee6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:26:16 +0100 Subject: [PATCH 021/344] Test and get things working --- .../modules/local/get_software_versions.nf | 48 +++++++++---------- 1 file changed, 24 insertions(+), 24 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf index 74f24823b4..1acadf8ad7 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf @@ -1,24 +1,24 @@ -/* - * Parse software version numbers - */ -process get_software_versions { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, - saveAs: { filename -> - if (filename.indexOf(".csv") > 0) filename - else null - } - - output: - file 'software_versions_mqc.yaml' - file "software_versions.csv" - - script: - // TODO nf-core: Get all tools to print their version number here - """ - echo $workflow.manifest.version > v_pipeline.txt - echo $workflow.nextflow.version > v_nextflow.txt - fastqc --version > v_fastqc.txt - multiqc --version > v_multiqc.txt - scrape_software_versions.py &> software_versions_mqc.yaml - """ -} +/* + * Parse software version numbers + */ +process GET_SOFTWARE_VERSIONS { + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, + saveAs: { filename -> + if (filename.indexOf(".csv") > 0) filename + else null + } + + output: + path 'software_versions_mqc.yaml' + path "software_versions.csv" + + script: + // TODO nf-core: Get all tools to print their version number here + """ + echo $workflow.manifest.version > v_pipeline.txt + echo $workflow.nextflow.version > v_nextflow.txt + fastqc --version > v_fastqc.txt + multiqc --version > v_multiqc.txt + scrape_software_versions.py &> software_versions_mqc.yaml + """ +} From c705f47e4cc43dd27d9fa4b799922eee1a570547 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:26:23 +0100 Subject: [PATCH 022/344] Test and get things working --- .../modules/local/output_documentation.nf | 36 +++++++++---------- 1 file changed, 18 insertions(+), 18 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf index 37688ff65c..b6d24b0f58 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf @@ -1,18 +1,18 @@ -/* - * Output description HTML - */ -process output_documentation { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode - - input: - file output_docs from ch_output_docs - file images from ch_output_docs_images - - output: - file "results_description.html" - - script: - """ - markdown_to_html.py $output_docs -o results_description.html - """ -} +/* + * Output Markdown documentation to HTML + */ +process OUTPUT_DOCUMENTATION { + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode + + input: + path output_docs + path images + + output: + path "results_description.html" + + script: + """ + markdown_to_html.py $output_docs -o results_description.html + """ +} From d47ece01c6ea3785682fbb2eaac92c4c116542f6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:26:32 +0100 Subject: [PATCH 023/344] Test and get things working --- .../modules/nf-core/fastqc.nf | 24 +++++++++++++------ 1 file changed, 17 insertions(+), 7 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf index 463b9dcfa8..8b30a6c6fd 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf @@ -1,7 +1,7 @@ /* * FastQC */ -process fastqc { +process FASTQC { tag "$name" label 'process_medium' publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, @@ -10,14 +10,24 @@ process fastqc { } input: - set val(name), file(reads) from ch_read_files_fastqc + tuple val(name), val(single_end), path(reads) output: - file "*_fastqc.{zip,html}" into ch_fastqc_results + path "*.{zip,html}" script: - """ - fastqc --quiet --threads $task.cpus $reads - """ + // Add soft-links to original FastQs for consistent naming in pipeline + if (single_end) { + """ + [ ! -f ${name}.fastq.gz ] && ln -s $reads ${name}.fastq.gz + fastqc --quiet --threads $task.cpus ${name}.fastq.gz + """ + } else { + """ + [ ! -f ${name}_1.fastq.gz ] && ln -s ${reads[0]} ${name}_1.fastq.gz + [ ! -f ${name}_2.fastq.gz ] && ln -s ${reads[1]} ${name}_2.fastq.gz + fastqc --quiet --threads $task.cpus ${name}_1.fastq.gz + fastqc --quiet --threads $task.cpus ${name}_2.fastq.gz + """ + } } - From 8fc7226463fac9126aee2aa767c6c43c71cbd75a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:26:46 +0100 Subject: [PATCH 024/344] Move relevant code here for now --- .../modules/nf-core/multiqc.nf | 43 ++++++++++++++----- 1 file changed, 33 insertions(+), 10 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index 8322f82abc..7a44eae11a 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -1,21 +1,44 @@ +// Has the run name been specified by the user? +// this has the bonus effect of catching both -name and --name +custom_runName = params.name +if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { + custom_runName = workflow.runName +} + +// Channel.from(summary.collect{ [it.key, it.value] }) +// .map { k,v -> "
$k
${v ?: 'N/A'}
" } +// .reduce { a, b -> return [a, b].join("\n ") } +// .map { x -> """ +// id: '{{ cookiecutter.name_noslash }}-summary' +// description: " - this information is collected when the pipeline is started." +// section_name: '{{ cookiecutter.name }} Workflow Summary' +// section_href: 'https://github.com/{{ cookiecutter.name }}' +// plot_type: 'html' +// data: | +//
+// $x +//
+// """.stripIndent() } +// .set { ch_workflow_summary } + /* - * STEP 2 - MultiQC + * MultiQC */ process MULTIQC { - publishDir "${params.outdir}/MultiQC", mode: params.publish_dir_mode + publishDir "${params.outdir}/multiqc", mode: params.publish_dir_mode input: - file (multiqc_config) from ch_multiqc_config - file (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) + path (multiqc_config) from ch_multiqc_config + path (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) // TODO nf-core: Add in log files from your new processes for MultiQC to find! - file ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) - file ('software_versions/*') from ch_software_versions_yaml.collect() - file workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") + path ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) + path ('software_versions/*') from ch_software_versions_yaml.collect() + path workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") output: - file "*multiqc_report.html" into ch_multiqc_report - file "*_data" - file "multiqc_plots" + path "*multiqc_report.html" + path "*_data" + path "multiqc_plots" script: rtitle = custom_runName ? "--title \"$custom_runName\"" : '' From 3dc408c5e73cdd62ba752123915983ed19508e89 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:27:06 +0100 Subject: [PATCH 025/344] Use viralrecon testdata --- .../{{cookiecutter.name_noslash}}/conf/test.config | 9 ++++----- .../{{cookiecutter.name_noslash}}/conf/test_full.config | 9 ++++----- 2 files changed, 8 insertions(+), 10 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config index 7840d28846..a9a282735e 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config @@ -18,9 +18,8 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false - input_paths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] - ] + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_amplicon.csv' + + // Genome references + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.fna.gz' } diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config index 170c6bd4fc..5cc0a683e3 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config @@ -14,9 +14,8 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - single_end = false - readPaths = [ - ['Testdata', ['https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R1.tiny.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/exoseq/testdata/Testdata_R2.tiny.fastq.gz']], - ['SRR389222', ['https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub1.fastq.gz', 'https://github.com/nf-core/test-datasets/raw/methylseq/testdata/SRR389222_sub2.fastq.gz']] - ] + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_amplicon.csv' + + // Genome references + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.fna.gz' } From bbeb24d62c0d148bd9ea7118db68cfa005b97720 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:27:22 +0100 Subject: [PATCH 026/344] Remove single_end and update input default --- .../{{cookiecutter.name_noslash}}/nextflow.config | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index 30f5260f67..5f422b177c 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -11,8 +11,7 @@ params { // Workflow flags // TODO nf-core: Specify your pipeline's command line flags genome = false - input = "data/*{1,2}.fastq.gz" - single_end = false + input = './samplesheet.csv' outdir = './results' publish_dir_mode = 'copy' From c5fcec2504b1da58d64909bd9de3543e2aae3a80 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:27:29 +0100 Subject: [PATCH 027/344] Remove single_end and update input default --- .../nextflow_schema.json | 13 +++---------- 1 file changed, 3 insertions(+), 10 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json index c753f17ae8..864a0a7c5a 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json @@ -10,16 +10,9 @@ "properties": { "input": { "type": "string", - "fa_icon": "fas fa-dna", - "description": "Input FastQ files.", - "help_text": "A glob pattern for input FastQ files. Should include at least one asterisk (*). For paired-end data, should contain curly brackets with two patterns differentiating the paired reads e.g. `*_R{1,2}.fastq.gz`" - }, - "single_end": { - "type": "boolean", - "description": "Specifies that the input is single-end reads.", - "fa_icon": "fas fa-align-center", - "default": false, - "help_text": "By default, the pipeline expects paired-end data. If you have single-end data, specify this parameter on the command line when you launch the pipeline. It is not possible to run a mixture of single-end and paired-end files in one run." + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a samplesheet with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/{{ cookiecutter.short_name }}/docs/usage#--input).", + "fa_icon": "fas fa-file-csv" }, "outdir": { "type": "string", From 5f022f537a8ed0fecff7b835b523c9f6aba9b110 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:27:46 +0100 Subject: [PATCH 028/344] Get things working end-to-end. Wooohooooo --- .../{{cookiecutter.name_noslash}}/main.nf | 95 +++++++++---------- 1 file changed, 44 insertions(+), 51 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index ce878ce7a8..fea7f1f5da 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -14,14 +14,14 @@ nextflow.preview.dsl = 2 /* * Print help message if required */ -include 'modules/nf-core/functions' params(params) +include { print_help; check_genome; check_hostname; check_awsbatch; create_summary; send_email } from './modules/local/functions' if (params.help) { print_help() exit 0 } /* - * Stage and check config files + * Stage config files */ ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() @@ -29,28 +29,21 @@ ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true) /* - * Create a channel for input read files + * Validate parameters */ -if (params.input_paths) { - if (params.single_end) { - Channel - .from(params.input_paths) - .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } - .into { ch_read_files_fastqc; ch_read_files_trimming } - } else { - Channel - .from(params.input_paths) - .map { row -> [ row[0], [ file(row[1][0], checkIfExists: true), file(row[1][1], checkIfExists: true) ] ] } - .ifEmpty { exit 1, "params.input_paths was empty - no input files supplied" } - .into { ch_read_files_fastqc; ch_read_files_trimming } - } -} else { - Channel - .fromFilePairs(params.input, size: params.single_end ? 1 : 2) - .ifEmpty { exit 1, "Cannot find any reads matching: ${params.input}\nNB: Path needs to be enclosed in quotes!\nIf this is single-end data, please specify --single_end on the command line." } - .into { ch_read_files_fastqc; ch_read_files_trimming } -} +if (params.input) { ch_input = file(params.input, checkIfExists: true) } else { exit 1, "Input samplesheet file not specified!" } + +/* + * Reference genomes + */ +// TODO nf-core: Add any reference files that are needed +// NOTE - FOR SIMPLICITY THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY +// If you want to use the channel below in a process, define the following: +// input: +// file fasta from ch_fasta +// +params.fasta = params.genomes[params.genome]?.fasta +if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } /* * Check and print summary for parameters @@ -61,26 +54,42 @@ check_awsbatch() // Check AWS batch settings summary = create_summary() // Print parameter summary /* - * FastQC + * Include local pipeline modules */ -//include 'modules/nf-core/fastqc' params(params) +include { OUTPUT_DOCUMENTATION } from './modules/local/output_documentation' params(params) +include { GET_SOFTWARE_VERSIONS } from './modules/local/get_software_versions' params(params) +include { CHECK_SAMPLESHEET; check_samplesheet_paths } from './modules/local/check_samplesheet' params(params) /* - * MultQC + * Include nf-core modules */ -//include 'modules/nf-core/multiqc' params(params) +include { FASTQC } from './modules/nf-core/fastqc' params(params) +include { MULTIQC } from './modules/nf-core/multiqc' params(params) /* - * Get software versions + * Run the workflow */ -include 'modules/nf-core/get_software_versions' params(params) -get_software_versions() +workflow { -/* - * Output markdown documentation as HTML - */ -include 'modules/nf-core/output_documentation' params(params) -output_documentation() + CHECK_SAMPLESHEET(ch_input) + .splitCsv(header:true, sep:',') + .map { check_samplesheet_paths(it) } + .set { ch_raw_reads } + + FASTQC(ch_raw_reads) + + OUTPUT_DOCUMENTATION( + ch_output_docs, + ch_output_docs_images) + + GET_SOFTWARE_VERSIONS() + + // MULTIQC( + // summary, + // fastqc.out, + // ch_multiqc_config + // ) +} /* * Send completion email @@ -88,19 +97,3 @@ output_documentation() workflow.onComplete { send_email(summary) } - -// Channel.from(summary.collect{ [it.key, it.value] }) -// .map { k,v -> "
$k
${v ?: 'N/A'}
" } -// .reduce { a, b -> return [a, b].join("\n ") } -// .map { x -> """ -// id: '{{ cookiecutter.name_noslash }}-summary' -// description: " - this information is collected when the pipeline is started." -// section_name: '{{ cookiecutter.name }} Workflow Summary' -// section_href: 'https://github.com/{{ cookiecutter.name }}' -// plot_type: 'html' -// data: | -//
-// $x -//
-// """.stripIndent() } -// .set { ch_workflow_summary } From 8a26311a7d807bbd68fff594850615f9efc7c704 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:27:54 +0100 Subject: [PATCH 029/344] Initial commit --- .../bin/check_samplesheet.py | 115 ++++++++++++++++++ 1 file changed, 115 insertions(+) create mode 100755 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py new file mode 100755 index 0000000000..f24329efb3 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -0,0 +1,115 @@ +#!/usr/bin/env python + +import os +import sys +import errno +import argparse + +def parse_args(args=None): + Description = 'Reformat {{ cookiecutter.name }} samplesheet file and check its contents.' + Epilog = """Example usage: python check_samplesheet.py """ + + parser = argparse.ArgumentParser(description=Description, epilog=Epilog) + parser.add_argument('FILE_IN', help="Input samplesheet file.") + parser.add_argument('FILE_OUT', help="Output file.") + return parser.parse_args(args) + + +def make_dir(path): + if not len(path) == 0: + try: + os.makedirs(path) + except OSError as exception: + if exception.errno != errno.EEXIST: + raise + + +def print_error(error,line): + print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error,line.strip())) + sys.exit(1) + + +def check_samplesheet(FileIn,FileOut): + ## Check header + HEADER = ['sample', 'fastq_1', 'fastq_2'] + fin = open(FileIn,'r') + header = fin.readline().strip().split(',') + if header != HEADER: + print("ERROR: Please check samplesheet header -> {} != {}".format(','.join(header),','.join(HEADER))) + sys.exit(1) + + sampleRunDict = {} + while True: + line = fin.readline() + if line: + lspl = [x.strip() for x in line.strip().split(',')] + + ## Check valid number of columns per row + if len(lspl) != len(header): + print_error("Invalid number of columns (minimum = {})!".format(len(header)),line) + + numCols = len([x for x in lspl if x]) + if numCols < 2: + print_error("Invalid number of populated columns (minimum = 2)!".format(line)) + + ## Check sample name entries + sample,fastQFiles = lspl[0],lspl[1:] + if sample: + if sample.find(' ') != -1: + print_error("Sample entry contains spaces!",line) + else: + print_error("Sample entry has not been specified!",line) + + ## Check FastQ file extension + for fastq in fastQFiles: + if fastq: + if fastq.find(' ') != -1: + print_error("FastQ file contains spaces!",line) + if fastq[-9:] != '.fastq.gz' and fastq[-6:] != '.fq.gz': + print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",line) + + ## Auto-detect paired-end/single-end + sample_info = [] ## [single_end, fastq_1, fastq_2] + fastq_1,fastq_2 = fastQFiles + if sample and fastq_1 and fastq_2: ## Paired-end short reads + sample_info = ['0', fastq_1, fastq_2] + elif sample and fastq_1 and not fastq_2: ## Single-end short reads + sample_info = ['1', fastq_1, fastq_2] + else: + print_error("Invalid combination of columns provided!",line) + + if sample not in sampleRunDict: + sampleRunDict[sample] = [sample_info] + else: + if sample_info in sampleRunDict[sample]: + print_error("Samplesheet contains duplicate rows!",line) + else: + sampleRunDict[sample].append(sample_info) + else: + fin.close() + break + + ## Write validated samplesheet with appropriate columns + if len(sampleRunDict) > 0: + OutDir = os.path.dirname(FileOut) + make_dir(OutDir) + fout = open(FileOut,'w') + fout.write(','.join(['sample', 'single_end', 'fastq_1', 'fastq_2']) + '\n') + for sample in sorted(sampleRunDict.keys()): + + ## Check that multiple runs of the same sample are of the same datatype + if not all(x[0] == sampleRunDict[sample][0][0] for x in sampleRunDict[sample]): + print_error("Multiple runs of a sample must be of the same datatype","Sample: {}".format(sample)) + + for idx,val in enumerate(sampleRunDict[sample]): + fout.write(','.join(["{}_T{}".format(sample,idx+1)] + val) + '\n') + fout.close() + + +def main(args=None): + args = parse_args(args) + check_samplesheet(args.FILE_IN,args.FILE_OUT) + + +if __name__ == '__main__': + sys.exit(main()) From 4ae3b42e5a0e9f28acce9cf53f91b658d6b8c1bd Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:27:59 +0100 Subject: [PATCH 030/344] Initial commit --- .../modules/local/check_samplesheet.nf | 34 +++++++++++++++++++ 1 file changed, 34 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf new file mode 100644 index 0000000000..8eb356f762 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf @@ -0,0 +1,34 @@ +/* + * Reformat input samplesheet and check validity + */ +process CHECK_SAMPLESHEET { + tag "$samplesheet" + publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode + + input: + path samplesheet + + output: + path '*.csv' + + script: // This script is bundled with the pipeline, in {{ cookiecutter.name }}/bin/ + """ + check_samplesheet.py $samplesheet samplesheet.valid.csv + """ +} + +// Function to get list of [ sample, single_end?, [ fastq_1, fastq_2 ] ] +def check_samplesheet_paths(LinkedHashMap samplesheet) { + def sample = samplesheet.sample + def single_end = samplesheet.single_end.toBoolean() + def fastq_1 = samplesheet.fastq_1 + def fastq_2 = samplesheet.fastq_2 + + def array = [] + if (single_end) { + array = [ sample, single_end, [ file(fastq_1, checkIfExists: true) ] ] + } else { + array = [ sample, single_end, [ file(fastq_1, checkIfExists: true), file(fastq_2, checkIfExists: true) ] ] + } + return array +} From 7728296643722b55842cce0443833055774f20ae Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 13:50:51 +0100 Subject: [PATCH 031/344] Fix launch lint --- tests/test_launch.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/test_launch.py b/tests/test_launch.py index 33d310055d..e0d8138f1d 100644 --- a/tests/test_launch.py +++ b/tests/test_launch.py @@ -210,11 +210,11 @@ def test_sanitise_web_response(self): """ Check that we can properly sanitise results from the web """ self.launcher.get_pipeline_schema() self.launcher.nxf_flags['-name'] = '' - self.launcher.schema_obj.input_params['single_end'] = 'true' + self.launcher.schema_obj.input_params['igenomes_ignore'] = 'true' self.launcher.schema_obj.input_params['max_cpus'] = '12' self.launcher.sanitise_web_response() assert '-name' not in self.launcher.nxf_flags - assert self.launcher.schema_obj.input_params['single_end'] == True + assert self.launcher.schema_obj.input_params['igenomes_ignore'] == True assert self.launcher.schema_obj.input_params['max_cpus'] == 12 def test_ob_to_pyinquirer_bool(self): From 08fbf9627ec51fbbaee2fd640b030c3b276c2844 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 7 Jul 2020 15:23:09 +0100 Subject: [PATCH 032/344] Update nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py Co-authored-by: Phil Ewels --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index f24329efb3..de8a37c2c8 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -21,7 +21,7 @@ def make_dir(path): os.makedirs(path) except OSError as exception: if exception.errno != errno.EEXIST: - raise + raise exception def print_error(error,line): From 7f95f82469df15d0cc6f74e6c8cb1bcc29070b10 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 7 Jul 2020 15:23:57 +0100 Subject: [PATCH 033/344] Update nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py Co-authored-by: Phil Ewels --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index de8a37c2c8..8e2558ec3d 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -16,7 +16,7 @@ def parse_args(args=None): def make_dir(path): - if not len(path) == 0: + if len(path) > 0: try: os.makedirs(path) except OSError as exception: From b8f4caf723854ca95692157461b1f34b305db5fb Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 7 Jul 2020 15:24:30 +0100 Subject: [PATCH 034/344] Update nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py Co-authored-by: Phil Ewels --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 8e2558ec3d..a0052de353 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -65,7 +65,7 @@ def check_samplesheet(FileIn,FileOut): if fastq: if fastq.find(' ') != -1: print_error("FastQ file contains spaces!",line) - if fastq[-9:] != '.fastq.gz' and fastq[-6:] != '.fq.gz': + if not fastq.endswith('.fastq.gz') and not fastq.endswith('.fq.gz'): print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",line) ## Auto-detect paired-end/single-end From ed56f660695fa54697c3d802864a47836bb53c15 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 7 Jul 2020 15:39:27 +0100 Subject: [PATCH 035/344] Update nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf Co-authored-by: Phil Ewels --- .../{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf index 8b30a6c6fd..5129ab4884 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf @@ -26,8 +26,7 @@ process FASTQC { """ [ ! -f ${name}_1.fastq.gz ] && ln -s ${reads[0]} ${name}_1.fastq.gz [ ! -f ${name}_2.fastq.gz ] && ln -s ${reads[1]} ${name}_2.fastq.gz - fastqc --quiet --threads $task.cpus ${name}_1.fastq.gz - fastqc --quiet --threads $task.cpus ${name}_2.fastq.gz + fastqc --quiet --threads $task.cpus ${name}_1.fastq.gz ${name}_2.fastq.gz """ } } From 06e665b3922f7f2833bfe4551b5427f8ec9d2b99 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 15:52:47 +0100 Subject: [PATCH 036/344] Change variable case --- .../bin/check_samplesheet.py | 40 +++++++++---------- 1 file changed, 20 insertions(+), 20 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index a0052de353..1015624614 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -7,7 +7,7 @@ def parse_args(args=None): Description = 'Reformat {{ cookiecutter.name }} samplesheet file and check its contents.' - Epilog = """Example usage: python check_samplesheet.py """ + Epilog = 'Example usage: python check_samplesheet.py ' parser = argparse.ArgumentParser(description=Description, epilog=Epilog) parser.add_argument('FILE_IN', help="Input samplesheet file.") @@ -29,16 +29,16 @@ def print_error(error,line): sys.exit(1) -def check_samplesheet(FileIn,FileOut): +def check_samplesheet(file_in,file_out): ## Check header HEADER = ['sample', 'fastq_1', 'fastq_2'] - fin = open(FileIn,'r') + fin = open(file_in,'r') header = fin.readline().strip().split(',') if header != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(','.join(header),','.join(HEADER))) sys.exit(1) - sampleRunDict = {} + sample_run_dict = {} while True: line = fin.readline() if line: @@ -48,12 +48,12 @@ def check_samplesheet(FileIn,FileOut): if len(lspl) != len(header): print_error("Invalid number of columns (minimum = {})!".format(len(header)),line) - numCols = len([x for x in lspl if x]) - if numCols < 2: + num_cols = len([x for x in lspl if x]) + if num_cols < 2: print_error("Invalid number of populated columns (minimum = 2)!".format(line)) ## Check sample name entries - sample,fastQFiles = lspl[0],lspl[1:] + sample,fastq_files = lspl[0],lspl[1:] if sample: if sample.find(' ') != -1: print_error("Sample entry contains spaces!",line) @@ -61,7 +61,7 @@ def check_samplesheet(FileIn,FileOut): print_error("Sample entry has not been specified!",line) ## Check FastQ file extension - for fastq in fastQFiles: + for fastq in fastq_files: if fastq: if fastq.find(' ') != -1: print_error("FastQ file contains spaces!",line) @@ -70,7 +70,7 @@ def check_samplesheet(FileIn,FileOut): ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2] - fastq_1,fastq_2 = fastQFiles + fastq_1,fastq_2 = fastq_files if sample and fastq_1 and fastq_2: ## Paired-end short reads sample_info = ['0', fastq_1, fastq_2] elif sample and fastq_1 and not fastq_2: ## Single-end short reads @@ -78,30 +78,30 @@ def check_samplesheet(FileIn,FileOut): else: print_error("Invalid combination of columns provided!",line) - if sample not in sampleRunDict: - sampleRunDict[sample] = [sample_info] + if sample not in sample_run_dict: + sample_run_dict[sample] = [sample_info] else: - if sample_info in sampleRunDict[sample]: + if sample_info in sample_run_dict[sample]: print_error("Samplesheet contains duplicate rows!",line) else: - sampleRunDict[sample].append(sample_info) + sample_run_dict[sample].append(sample_info) else: fin.close() break ## Write validated samplesheet with appropriate columns - if len(sampleRunDict) > 0: - OutDir = os.path.dirname(FileOut) - make_dir(OutDir) - fout = open(FileOut,'w') + if len(sample_run_dict) > 0: + out_dir = os.path.dirname(file_out) + make_dir(out_dir) + fout = open(file_out,'w') fout.write(','.join(['sample', 'single_end', 'fastq_1', 'fastq_2']) + '\n') - for sample in sorted(sampleRunDict.keys()): + for sample in sorted(sample_run_dict.keys()): ## Check that multiple runs of the same sample are of the same datatype - if not all(x[0] == sampleRunDict[sample][0][0] for x in sampleRunDict[sample]): + if not all(x[0] == sample_run_dict[sample][0][0] for x in sample_run_dict[sample]): print_error("Multiple runs of a sample must be of the same datatype","Sample: {}".format(sample)) - for idx,val in enumerate(sampleRunDict[sample]): + for idx,val in enumerate(sample_run_dict[sample]): fout.write(','.join(["{}_T{}".format(sample,idx+1)] + val) + '\n') fout.close() From a5882f952b99abfa281b7f0202907e5cc1c1e55a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 7 Jul 2020 16:11:52 +0100 Subject: [PATCH 037/344] Replace for a while --- .../bin/check_samplesheet.py | 26 +++++++++---------- 1 file changed, 12 insertions(+), 14 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 1015624614..7c195441b5 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -7,7 +7,7 @@ def parse_args(args=None): Description = 'Reformat {{ cookiecutter.name }} samplesheet file and check its contents.' - Epilog = 'Example usage: python check_samplesheet.py ' + Epilog = """Example usage: python check_samplesheet.py """ parser = argparse.ArgumentParser(description=Description, epilog=Epilog) parser.add_argument('FILE_IN', help="Input samplesheet file.") @@ -30,18 +30,19 @@ def print_error(error,line): def check_samplesheet(file_in,file_out): - ## Check header - HEADER = ['sample', 'fastq_1', 'fastq_2'] - fin = open(file_in,'r') - header = fin.readline().strip().split(',') - if header != HEADER: - print("ERROR: Please check samplesheet header -> {} != {}".format(','.join(header),','.join(HEADER))) - sys.exit(1) sample_run_dict = {} - while True: - line = fin.readline() - if line: + with open(file_in, 'r') as fin: + + ## Check header + HEADER = ['sample', 'fastq_1', 'fastq_2'] + header = fin.readline().strip().split(',') + if header != HEADER: + print("ERROR: Please check samplesheet header -> {} != {}".format(','.join(header),','.join(HEADER))) + sys.exit(1) + + ## Check sample entries + for line in fin: lspl = [x.strip() for x in line.strip().split(',')] ## Check valid number of columns per row @@ -85,9 +86,6 @@ def check_samplesheet(file_in,file_out): print_error("Samplesheet contains duplicate rows!",line) else: sample_run_dict[sample].append(sample_info) - else: - fin.close() - break ## Write validated samplesheet with appropriate columns if len(sample_run_dict) > 0: From 76166e4eee4f5fc3ce87ab4e6092ce299dae5151 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Sun, 12 Jul 2020 16:24:25 +0200 Subject: [PATCH 038/344] add TODO check_samplesheet --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 7c195441b5..da53b52ba7 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -34,6 +34,7 @@ def check_samplesheet(file_in,file_out): sample_run_dict = {} with open(file_in, 'r') as fin: + # TODO nf-core: update the header names for the input samplesheet ## Check header HEADER = ['sample', 'fastq_1', 'fastq_2'] header = fin.readline().strip().split(',') @@ -82,10 +83,7 @@ def check_samplesheet(file_in,file_out): if sample not in sample_run_dict: sample_run_dict[sample] = [sample_info] else: - if sample_info in sample_run_dict[sample]: - print_error("Samplesheet contains duplicate rows!",line) - else: - sample_run_dict[sample].append(sample_info) + print_error("Samplesheet contains duplicate samples!",line) ## Write validated samplesheet with appropriate columns if len(sample_run_dict) > 0: From 002fdac78f766ee9161fde01178d965d167268ae Mon Sep 17 00:00:00 2001 From: ggabernet Date: Sun, 12 Jul 2020 16:41:26 +0200 Subject: [PATCH 039/344] apply review comments --- .../bin/check_samplesheet.py | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index da53b52ba7..bac1790ea4 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -1,4 +1,5 @@ #!/usr/bin/env python +# TODO nf-core: Update the script to check the samplesheet import os import sys @@ -34,7 +35,7 @@ def check_samplesheet(file_in,file_out): sample_run_dict = {} with open(file_in, 'r') as fin: - # TODO nf-core: update the header names for the input samplesheet + # TODO nf-core: Update the column names for the input samplesheet ## Check header HEADER = ['sample', 'fastq_1', 'fastq_2'] header = fin.readline().strip().split(',') @@ -83,7 +84,10 @@ def check_samplesheet(file_in,file_out): if sample not in sample_run_dict: sample_run_dict[sample] = [sample_info] else: - print_error("Samplesheet contains duplicate samples!",line) + if sample_info in sample_run_dict[sample]: + print_error("Samplesheet contains duplicate rows!",line) + else: + sample_run_dict[sample].append(sample_info) ## Write validated samplesheet with appropriate columns if len(sample_run_dict) > 0: From 52ebb05bc5bda23436251d22ee01da37d553b4e6 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Sun, 12 Jul 2020 16:53:44 +0200 Subject: [PATCH 040/344] sample sheet example --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index bac1790ea4..9365ca19cd 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -29,8 +29,12 @@ def print_error(error,line): print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error,line.strip())) sys.exit(1) - +# TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in,file_out): + """This check shampleseet function checks that the sample sheet follows the following structure: + sample, fastq_1, fastq_2 + sample1, Sample1.fastq.gz, Sample2.fastq.gz + """ sample_run_dict = {} with open(file_in, 'r') as fin: From 9c8657fb22158508028c258c3a53c9eb17a5ed34 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Sun, 12 Jul 2020 17:18:01 +0200 Subject: [PATCH 041/344] add link to example datasheet --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 1 + 1 file changed, 1 insertion(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 9365ca19cd..f8367c3e04 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -1,5 +1,6 @@ #!/usr/bin/env python # TODO nf-core: Update the script to check the samplesheet +# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_sispa.csv import os import sys From 248780d8f4d8fd78a9f5003a8c507e0212b1529b Mon Sep 17 00:00:00 2001 From: ggabernet Date: Sun, 12 Jul 2020 17:25:47 +0200 Subject: [PATCH 042/344] fix spaces example --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index f8367c3e04..9ce1e82473 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -33,8 +33,8 @@ def print_error(error,line): # TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in,file_out): """This check shampleseet function checks that the sample sheet follows the following structure: - sample, fastq_1, fastq_2 - sample1, Sample1.fastq.gz, Sample2.fastq.gz + sample,fastq_1,fastq_2 + sample1,Sample1.fastq.gz,Sample2.fastq.gz """ sample_run_dict = {} From 0bdcdc799d31fbf39697b60e48c53349557fd431 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Sun, 12 Jul 2020 21:09:02 +0200 Subject: [PATCH 043/344] update base.config --- .../{{cookiecutter.name_noslash}}/conf/base.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config index 2a2322c95d..580f505c1e 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config @@ -44,7 +44,7 @@ process { withLabel:process_long { time = { check_max( 20.h * task.attempt, 'time' ) } } - withName:get_software_versions { + withName:GET_SOFTWARE_VERSIONS { cache = false } From 32b6f4abe969e63b2ac474be56df7f04ed233b6f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:19:33 +0100 Subject: [PATCH 044/344] Move boilerplate code to Groovy lib --- .../lib/CommonFunctions.groovy | 248 ------------------ .../lib/checks.groovy | 2 - .../lib/headers.groovy | 2 - .../lib/params.groovy | 2 - .../{{cookiecutter.name_noslash}}/main.nf | 43 ++- 5 files changed, 30 insertions(+), 267 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy deleted file mode 100644 index d7ffd457e4..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/CommonFunctions.groovy +++ /dev/null @@ -1,248 +0,0 @@ -/** This file holds several functions that can be used across many nf-core pipelines arbitrarily without having the main code in the pipelines specific repository in the future. Based on work by Patrick Huether (@phue), some updates and cleanup by (@apeltzer). -**/ - -@Grab('ch.qos.logback:logback-classic:1.2.3') - -import groovy.transform.CompileStatic -import groovy.json.JsonSlurper - -@groovy.util.logging.Slf4j -//@CompileStatic -class CommonFunctions { - - private static Map generateLogColors(Boolean monochromeLogs) { - Map colorcodes = [:] - colorcodes['reset'] = monochromeLogs ? '' : "\033[0m" - colorcodes['dim'] = monochromeLogs ? '' : "\033[2m" - colorcodes['black'] = monochromeLogs ? '' : "\033[0;30m" - colorcodes['green'] = monochromeLogs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochromeLogs ? '' : "\033[0;33m" - colorcodes['yellow_bold'] = monochromeLogs ? '' : "\033[1;93m" - colorcodes['blue'] = monochromeLogs ? '' : "\033[0;34m" - colorcodes['purple'] = monochromeLogs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochromeLogs ? '' : "\033[0;36m" - colorcodes['white'] = monochromeLogs ? '' : "\033[0;37m" - colorcodes['red'] = monochromeLogs ? '' : "\033[1;91m" - return colorcodes - } - - /* - * This method tries to read the params file from JSON settings file given a path to a schema. - * Can be directly used in the main.nf file to read in JSON schema and have a LinkedHashMap then with contents - e.g. def paramsWithUsage = CommonFunctions.readParamsFromJsonSettings("$baseDir/nextflow_schema.json") - */ - private static LinkedHashMap readParamsFromJsonSettings(String path) { - def paramsWithUsage = new LinkedHashMap() - try { - paramsWithUsage = tryReadParamsFromJsonSettings(path) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - paramsWithUsage = new LinkedHashMap() - } - return paramsWithUsage - } - - /* - Method to actually read in JSON file using Groovy. - Group (as Key), values are all parameters - - Parameter1 as Key, Description as Value - - Parameter2 as Key, Description as Value - .... - Group - - - */ - private static LinkedHashMap tryReadParamsFromJsonSettings(String path) throws Exception { - - def paramsContent = new File(path).text - def Map paramsWithUsage = (Map) new JsonSlurper().parseText(paramsContent).get('properties') - - /* Tree looks like this in nf-core schema - * properties <- this is what the first get('properties') gets us - group 1 - properties - description - group 2 - properties - description - group 3 - properties - description - */ - def output_map = new LinkedHashMap() - - //Lets go deeper - paramsWithUsage.each { key, val -> - //println "Group: $key\n" //Gets the Group names - def Map submap = paramsWithUsage."$key".properties //Gets the property object of the group - //println "Parameters:\n" //nested access to the Parameters - def sub_params = new LinkedHashMap() - submap.each { innerkey, value -> - //println "$innerkey\t$value.description" - sub_params.put("$innerkey", "$value.description") - } - output_map.put("$key", sub_params) - //println "\n" - } - return output_map - } - - static String helpMessage(paramsWithUsage, workflow) { - - def usageHelp = String.format( - """\ - Usage: - The typical command for running the pipeline is as follows: - nextflow run ${workflow.manifest.name} v${workflow.manifest.version} --input 'input.tsv' -profile docker - Options: - """.stripIndent(), println(prettyFormatJSON(paramsWithUsage))) - } - - static String prettyFormatJSON(paramsWithUsage){ - //Todo make this really nicer ... this is just experimental code - //ASCII codes seem to work fine - String output = "" - for(group in paramsWithUsage.keySet()) { - output += "Group: " + group + "\n" - def params = paramsWithUsage.get(group) //This gets the parameters of that particular group - for(par in params.keySet()) { - output+= "\u001B[1m" + par + "\u001B[1m" + "\t" + params.get(par) + "\n" - } - output += "\n" //Extra newline after parameter block - } - - return output - } - - static String nfcoreHeader(params, workflow) { - Map colors = generateLogColors(params.monochrome_logs) - def showHeader = String.format( - """\n - ${colors.dim}----------------------------------------------------${colors.reset} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} - ${colors.dim}----------------------------------------------------${colors.reset} - """.stripIndent()) - } - static void checkHostname(params) { - Map colors = generateLogColors(params.monochrome_logs) - if(params.hostnames){ - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if(hostname.contains(hname) && !workflow.profile.contains(prof)){ - log.error "====================================================\n" + - " ${colors.red}WARNING!${colors.reset} You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${colors.white}'$hostname'${colors.reset}\n" + - " ${colors.yellow_bold}It's highly recommended that you use `-profile $prof${colors.reset}`\n" + - "============================================================" - } - } - } - } - } - static void checkAWSbatch(params, workflow) { - - assert !params.awsqueue || !params.awsregion : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - assert !params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - assert workflow.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." - } - static void workflowReport(summary, workflow, params, baseDir, mqc_report) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if(!workflow.success){ - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if(workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if(workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if(workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - if(workflow.container) email_fields['summary']['Docker image'] = workflow.container - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$baseDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$baseDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: params.email, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.maxMultiqcEmailFileSize.toBytes() ] - def sf = new File("$baseDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - if (params.email) { - try { - if( params.plaintext_email ){ throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[$workflow.manifest.name] Sent summary e-mail to $params.email (sendmail)" - } catch (all) { - if (mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { - [ 'mail', '-s', subject, params.email, '-A', mqc_report ].execute() << email_txt - } else { - // Catch failures and try with plaintext - [ 'mail', '-s', subject, params.email ].execute() << email_txt - } - log.info "[$workflow.manifest.name] Sent summary e-mail to $params.email (mail)" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File( "${params.outdir}/pipeline_info/" ) - if( !output_d.exists() ) { - output_d.mkdirs() - } - def output_hf = new File( output_d, "pipeline_report.html" ) - output_hf.withWriter { w -> w << email_html } - def output_tf = new File( output_d, "pipeline_report.txt" ) - output_tf.withWriter { w -> w << email_txt } - - Map colors = generateLogColors(params.monochrome_logs) - - if (workflow.stats.ignoredCountFmt > 0 && workflow.success) { - log.info "${colors.purple}Warning, pipeline completed, but with errored process(es) ${colors.reset}" - log.info "${colors.red}Number of ignored errored process(es) : ${workflow.stats.ignoredCountFmt} ${colors.reset}" - log.info "${colors.green}Number of successfully ran process(es) : ${workflow.stats.succeedCountFmt} ${colors.reset}" - } - - if (workflow.success) { - log.info "${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}" - } else { - checkHostname(params) - log.info "${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}" - } - } -} - diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy deleted file mode 100644 index 2e3050433e..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/checks.groovy +++ /dev/null @@ -1,2 +0,0 @@ -/** This file holds several functions used to perform standard checks in the nf-core pipeline template e.g. hostname, AWS batch and genome checks. -**/ diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy deleted file mode 100644 index a90ede8c41..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/headers.groovy +++ /dev/null @@ -1,2 +0,0 @@ -/** This file holds several functions used to render the nf-core ANSI header. -**/ diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy deleted file mode 100644 index fe1880dd07..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/params.groovy +++ /dev/null @@ -1,2 +0,0 @@ -/** This file holds several functions used to perform JSON parameter validation, help and summary rendering in the nf-core pipeline template. -**/ diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index fea7f1f5da..13b784eeeb 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -14,9 +14,11 @@ nextflow.preview.dsl = 2 /* * Print help message if required */ -include { print_help; check_genome; check_hostname; check_awsbatch; create_summary; send_email } from './modules/local/functions' if (params.help) { - print_help() + // TODO nf-core: Update typical command used to run pipeline + def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv -profile docker" + log.info Headers.nf_core(workflow, params.monochrome_logs) + log.info JSON.params_help("$baseDir/nextflow_schema.json", command) exit 0 } @@ -37,21 +39,33 @@ if (params.input) { ch_input = file(params.input, checkIfExists: true) } else { * Reference genomes */ // TODO nf-core: Add any reference files that are needed -// NOTE - FOR SIMPLICITY THIS IS NOT USED IN THIS PIPELINE, EXAMPLE ONLY -// If you want to use the channel below in a process, define the following: -// input: -// file fasta from ch_fasta -// +// NOTE - FOR SIMPLICITY THIS IS NOT USED IN THIS PIPELINE +// EXAMPLE ONLY TO DEMONSTRATE USAGE OF AWS IGENOMES +if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" +} params.fasta = params.genomes[params.genome]?.fasta if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } /* - * Check and print summary for parameters + * Check parameters + */ +Checks.aws_batch(workflow, params) // Check AWS batch settings +Checks.hostname(workflow, params, log) // Check the hostnames against configured profiles + +/* + * Print parameter summary */ -check_genome() // Check if genome exists in the config file -check_hostname() // Check the hostnames against configured profiles -check_awsbatch() // Check AWS batch settings -summary = create_summary() // Print parameter summary +// Has the run name been specified by the user? +// this has the bonus effect of catching both -name and --name +run_name = params.name +if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { + run_name = workflow.runName +} +summary = JSON.params_summary(workflow, params, run_name) +log.info Headers.nf_core(workflow, params.monochrome_logs) +log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") +log.info "-\033[2m----------------------------------------------------\033[0m-" /* * Include local pipeline modules @@ -95,5 +109,8 @@ workflow { * Send completion email */ workflow.onComplete { - send_email(summary) + def multiqc_report = [] + Completion.email(workflow, params, summary, run_name, baseDir, multiqc_report, log) + Completion.summary(workflow, params, log) } + From 0313c75efab1d7d3c44dbff0efa08def0bfc643a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:20:00 +0100 Subject: [PATCH 045/344] Add Groovy Checks --- .../lib/Checks.groovy | 36 +++++++++++++++++++ 1 file changed, 36 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy new file mode 100644 index 0000000000..0c912c401b --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy @@ -0,0 +1,36 @@ +/* + * This file holds several functions used to perform standard checks for the nf-core pipeline template. + */ + +class Checks { + + static void aws_batch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + assert !params.awsqueue || !params.awsregion : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + // related: https://github.com/nextflow-io/nextflow/issues/813 + assert !params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + // Prevent trace files to be stored on S3 since S3 does not support rolling files. + assert params.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + } + } + + static void hostname(workflow, params, log) { + Map colors = Headers.log_colours(params.monochrome_logs) + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } + } + } + } + } +} + From cba6c4a05ad5f1ee6589eeeead1a7dabcb99d0fb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:20:11 +0100 Subject: [PATCH 046/344] Add Groovy Completion --- .../lib/Completion.groovy | 119 ++++++++++++++++++ 1 file changed, 119 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy new file mode 100644 index 0000000000..0a7a2b555d --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy @@ -0,0 +1,119 @@ +/* + * Functions to be run on completion of pipeline + */ + +class Completion { + static void email(workflow, params, summary, run_name, baseDir, multiqc_report, log) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = run_name ?: workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary + email_fields['summary']['Date Started'] = workflow.start + email_fields['summary']['Date Completed'] = workflow.complete + email_fields['summary']['Pipeline script file path'] = workflow.scriptFile + email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision + email_fields['summary']['Nextflow Version'] = workflow.nextflow.version + email_fields['summary']['Nextflow Build'] = workflow.nextflow.build + email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + mqc_report = mqc_report[0] + } + } + } catch (all) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$baseDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$baseDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] + def sf = new File("$baseDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "[$workflow.manifest.name] Sent summary e-mail to $email_address (sendmail)" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "[$workflow.manifest.name] Sent summary e-mail to $email_address (mail)" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + } + + static void summary(workflow, params, log) { + Map colors = Headers.log_colours(params.monochrome_logs) + if (workflow.stats.ignoredCount > 0 && workflow.success) { + log.info "-${colors.purple}Warning, pipeline completed, but with errored process(es) ${colors.reset}-" + log.info "-${colors.red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${colors.reset}-" + log.info "-${colors.green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${colors.reset}-" + } + if (workflow.success) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + Checks.hostname() + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } +} + From 1ffef6934e42800227cd1e58391427766b187792 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:20:24 +0100 Subject: [PATCH 047/344] Add Groovy Headers --- .../lib/Headers.groovy | 39 +++++++++++++++++++ 1 file changed, 39 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy new file mode 100644 index 0000000000..8fd9c8c6fc --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy @@ -0,0 +1,39 @@ +/* + * This file holds several functions used to render the nf-core ANSI header. + */ + +class Headers { + + private static Map log_colours(Boolean monochrome_logs) { + Map colorcodes = [:] + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" + return colorcodes + } + + static String nf_core(workflow, monochrome_logs) { + Map colors = log_colours(monochrome_logs) + String.format( + """\n + -${colors.dim}----------------------------------------------------${colors.reset}- + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + -${colors.dim}----------------------------------------------------${colors.reset}- + """.stripIndent() + ) + } +} + From 8231cbcab863bbc8698356bf0317d7da25424b7b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:20:36 +0100 Subject: [PATCH 048/344] Add Groovy JSON --- .../lib/JSON.groovy | 117 ++++++++++++++++++ 1 file changed, 117 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy new file mode 100644 index 0000000000..2b97a66aa0 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy @@ -0,0 +1,117 @@ +/* + * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. + */ + +import groovy.json.JsonSlurper + +class JSON { + /* + * This method tries to read a JSON params file + */ + private static LinkedHashMap params_get(String path) { + def usage = new LinkedHashMap() + try { + usage = params_try(path) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + usage = new LinkedHashMap() + } + return usage + } + + /* + Method to actually read in JSON file using Groovy. + Group (as Key), values are all parameters + - Parameter1 as Key, Description as Value + - Parameter2 as Key, Description as Value + .... + Group + - + */ + private static LinkedHashMap params_try(String path) throws Exception { + + def json = new File(path).text + def Map usage = (Map) new JsonSlurper().parseText(json).get('properties') + + /* Tree looks like this in nf-core schema + * properties <- this is what the first get('properties') gets us + group 1 + properties + description + group 2 + properties + description + group 3 + properties + description + */ + def output_map = new LinkedHashMap() + + //Lets go deeper + usage.each { key, val -> + //println "Group: $key\n" //Gets the Group names + def Map submap = usage."$key".properties //Gets the property object of the group + //println "Parameters:\n" //nested access to the Parameters + def sub_params = new LinkedHashMap() + submap.each { innerkey, value -> + //println "$innerkey\t$value.description" + sub_params.put("$innerkey", "$value.description") + } + output_map.put("$key", sub_params) + //println "\n" + } + return output_map + } + + static String params_help(path, command) { + String output = "Typical pipeline command:\n\n" + output += " ${command}\n\n" + output += params_beautify(params_get(path)) + } + + static String params_beautify(usage) { + String output = "" + for (group in usage.keySet()) { + output += group + "\n" + def params = usage.get(group) //This gets the parameters of that particular group + for (par in params.keySet()) { + output+= " \u001B[1m" + par.padRight(27) + "\u001B[1m" + params.get(par) + "\n" + } + output += "\n" //Extra newline after parameter block + } + return output + } + + private static LinkedHashMap params_summary(workflow, params, run_name) { + def Map summary = [:] + if (workflow.revision) summary['Pipeline Release'] = workflow.revision + summary['Run Name'] = run_name ?: workflow.runName + // TODO nf-core: Report custom parameters here + summary['Input'] = params.input + summary['Fasta File'] = params.fasta + summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" + if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" + summary['Output dir'] = params.outdir + summary['Launch dir'] = workflow.launchDir + summary['Working dir'] = workflow.workDir + summary['Script dir'] = workflow.projectDir + summary['User'] = workflow.userName + if (workflow.profile.contains('awsbatch')) { + summary['AWS Region'] = params.awsregion + summary['AWS Queue'] = params.awsqueue + summary['AWS CLI'] = params.awscli + } + summary['Config Profile'] = workflow.profile + if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description + if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact + if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url + summary['Config Files'] = workflow.configFiles.join(', ') + if (params.email || params.email_on_fail) { + summary['E-mail Address'] = params.email + summary['E-mail on failure'] = params.email_on_fail + summary['MultiQC maxsize'] = params.max_multiqc_email_size + } + return summary + } +} + From fa68f801b1a606b182c39722edabc893dc83787b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:24:20 +0100 Subject: [PATCH 049/344] Update CHANGELOG --- CHANGELOG.md | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 03cda30cf5..a29269b857 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -55,7 +55,8 @@ GitHub or on the nf-core [`#json-schema` Slack channel](https://nfcore.slack.com * Add information about config files used for workflow execution (`workflow.configFiles`) to summary * Fix `markdown_to_html.py` to work with Python 2 and 3. * Change `params.reads` -> `params.input` -* Change `params.readPaths` -> `params.input_paths` +* Move, modularise and import boilerplate code via Groovy `lib/` directory +* Implement DSL 2 syntax for pipeline template ### Linting From b0ace52e8022a501d8924553ef92a6735221f97d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:36:31 +0100 Subject: [PATCH 050/344] Moved boilerplate functions to Groovy libs --- .../modules/local/functions.nf | 278 ------------------ 1 file changed, 278 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf deleted file mode 100644 index 40935217ce..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/functions.nf +++ /dev/null @@ -1,278 +0,0 @@ -// Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name -custom_runName = params.name -if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - custom_runName = workflow.runName -} - -/* - * Print help - */ -def print_help() { - // TODO nf-core: Add to this help message with new command line parameters - log.info nfcore_header() - log.info""" - - Usage: - - The typical command for running the pipeline is as follows: - - nextflow run {{ cookiecutter.name }} --input samplesheet.csv -profile docker - - Mandatory arguments: - --input [file] Comma-separated file containing information about the samples in the experiment (see docs/usage.md) - -profile [str] Configuration profile to use. Can use multiple (comma separated) - Available: conda, docker, singularity, test, awsbatch, and more - - Options: - --genome [str] Name of iGenomes reference - - References If not specified in the configuration file or you wish to overwrite any of the references - --fasta [file] Path to fasta reference - - Other options: - --outdir [file] The output directory where the results will be saved - --publish_dir_mode [str] Mode for publishing results in the output directory. Available: symlink, rellink, link, copy, copyNoFollow, move (Default: copy) - --email [email] Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits - --email_on_fail [email] Same as --email, except only send mail if the workflow is not successful - --max_multiqc_email_size [str] Threshold size for MultiQC report to be attached in notification email. If file generated by pipeline exceeds the threshold, it will not be attached (Default: 25MB) - -name [str] Name for the pipeline run. If not specified, Nextflow will automatically generate a random mnemonic - - AWSBatch options: - --awsqueue [str] The AWSBatch JobQueue that needs to be set when running on AWSBatch - --awsregion [str] The AWS Region for your AWS Batch job to run on - --awscli [str] Path to the AWS CLI tool - """.stripIndent() -} - -/* - * Check if genome exists in the config file - */ -def check_genome() { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" - } -} - -/* - * Print parameter summary - */ -def create_summary() { - // Header log info - log.info nfcore_header() - def summary = [:] - if (workflow.revision) summary['Pipeline Release'] = workflow.revision - summary['Run Name'] = custom_runName ?: workflow.runName - // TODO nf-core: Report custom parameters here - summary['Samplesheet'] = params.input - summary['Fasta File'] = params.fasta - summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" - if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" - summary['Output dir'] = params.outdir - summary['Launch dir'] = workflow.launchDir - summary['Working dir'] = workflow.workDir - summary['Script dir'] = workflow.projectDir - summary['User'] = workflow.userName - if (workflow.profile.contains('awsbatch')) { - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue - summary['AWS CLI'] = params.awscli - } - summary['Config Profile'] = workflow.profile - if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description - if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact - if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url - summary['Config Files'] = workflow.configFiles.join(', ') - if (params.email || params.email_on_fail) { - summary['E-mail Address'] = params.email - summary['E-mail on failure'] = params.email_on_fail - summary['MultiQC maxsize'] = params.max_multiqc_email_size - } - log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") - log.info "-\033[2m--------------------------------------------------\033[0m-" - - return summary -} - -/* - * ANSI nf-core header - */ -def nfcore_header() { - // Log colors ANSI codes - c_black = params.monochrome_logs ? '' : "\033[0;30m"; - c_blue = params.monochrome_logs ? '' : "\033[0;34m"; - c_cyan = params.monochrome_logs ? '' : "\033[0;36m"; - c_dim = params.monochrome_logs ? '' : "\033[2m"; - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - c_white = params.monochrome_logs ? '' : "\033[0;37m"; - c_yellow = params.monochrome_logs ? '' : "\033[0;33m"; - - return """ -${c_dim}--------------------------------------------------${c_reset}- - ${c_green},--.${c_black}/${c_green},-.${c_reset} - ${c_blue} ___ __ __ __ ___ ${c_green}/,-._.--~\'${c_reset} - ${c_blue} |\\ | |__ __ / ` / \\ |__) |__ ${c_yellow}} {${c_reset} - ${c_blue} | \\| | \\__, \\__/ | \\ |___ ${c_green}\\`-._,-`-,${c_reset} - ${c_green}`._,._,\'${c_reset} - ${c_purple} {{ cookiecutter.name }} v${workflow.manifest.version}${c_reset} - -${c_dim}--------------------------------------------------${c_reset}- - """.stripIndent() -} - -/* - * Check the hostnames against configured profiles - */ -def check_hostname() { - def c_reset = params.monochrome_logs ? '' : "\033[0m" - def c_white = params.monochrome_logs ? '' : "\033[0;37m" - def c_red = params.monochrome_logs ? '' : "\033[1;91m" - def c_yellow_bold = params.monochrome_logs ? '' : "\033[1;93m" - if (params.hostnames) { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.error "====================================================\n" + - " ${c_red}WARNING!${c_reset} You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${c_white}'$hostname'${c_reset}\n" + - " ${c_yellow_bold}It's highly recommended that you use `-profile $prof${c_reset}`\n" + - "============================================================" - } - } - } - } -} - -/* - * Check AWS batch settings - */ -def check_awsbatch() { - if (workflow.profile.contains('awsbatch')) { - // AWSBatch sanity checking - if (!params.awsqueue || !params.awsregion) exit 1, "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - if (!params.outdir.startsWith('s3:')) exit 1, "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - if (params.tracedir.startsWith('s3:')) exit 1, "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." - } -} - -/* - * Completion e-mail notification - */ -def send_email(summary) { - - // Set up the e-mail variables - def subject = "[{{ cookiecutter.name }}] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[{{ cookiecutter.name }}] FAILED: $workflow.runName" - } - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = custom_runName ?: workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = ch_multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList) { - log.warn "[{{ cookiecutter.name }}] Found multiple reports from process 'multiqc', will use only one" - mqc_report = mqc_report[0] - } - } - } catch (all) { - log.warn "[{{ cookiecutter.name }}] Could not attach MultiQC report to summary email" - } - - // Check if we are only sending emails on failure - email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$baseDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$baseDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$baseDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (sendmail)" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "[{{ cookiecutter.name }}] Sent summary e-mail to $email_address (mail)" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - - c_green = params.monochrome_logs ? '' : "\033[0;32m"; - c_purple = params.monochrome_logs ? '' : "\033[0;35m"; - c_red = params.monochrome_logs ? '' : "\033[0;31m"; - c_reset = params.monochrome_logs ? '' : "\033[0m"; - - if (workflow.stats.ignoredCount > 0 && workflow.success) { - log.info "-${c_purple}Warning, pipeline completed, but with errored process(es) ${c_reset}-" - log.info "-${c_red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${c_reset}-" - log.info "-${c_green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${c_reset}-" - } - - if (workflow.success) { - log.info "-${c_purple}[{{ cookiecutter.name }}]${c_green} Pipeline completed successfully${c_reset}-" - } else { - checkHostname() - log.info "-${c_purple}[{{ cookiecutter.name }}]${c_red} Pipeline completed with errors${c_reset}-" - } -} From d7d07e91c0d11088823663ac20546de8921bcd3a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:48:05 +0100 Subject: [PATCH 051/344] Trim description --- .../{{cookiecutter.name_noslash}}/lib/JSON.groovy | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy index 2b97a66aa0..43bb98882a 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy @@ -102,9 +102,9 @@ class JSON { summary['AWS CLI'] = params.awscli } summary['Config Profile'] = workflow.profile - if (params.config_profile_description) summary['Config Profile Description'] = params.config_profile_description - if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact - if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url + if (params.config_profile_description) summary['Config Profile Descr'] = params.config_profile_description + if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact + if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url summary['Config Files'] = workflow.configFiles.join(', ') if (params.email || params.email_on_fail) { summary['E-mail Address'] = params.email From 3ed7b67ea30e60b62b8b554461b73fbe1cd8e604 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:48:18 +0100 Subject: [PATCH 052/344] Minor tweaks --- .../{{cookiecutter.name_noslash}}/main.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 13b784eeeb..230a7aa6e0 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -50,8 +50,8 @@ if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } /* * Check parameters */ -Checks.aws_batch(workflow, params) // Check AWS batch settings -Checks.hostname(workflow, params, log) // Check the hostnames against configured profiles +Checks.aws_batch(workflow, params) // Check AWS batch settings +Checks.hostname(workflow, params, log) // Check the hostnames against configured profiles /* * Print parameter summary @@ -64,7 +64,7 @@ if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { } summary = JSON.params_summary(workflow, params, run_name) log.info Headers.nf_core(workflow, params.monochrome_logs) -log.info summary.collect { k,v -> "${k.padRight(18)}: $v" }.join("\n") +log.info summary.collect { k,v -> "${k.padRight(20)}: $v" }.join("\n") log.info "-\033[2m----------------------------------------------------\033[0m-" /* @@ -100,7 +100,7 @@ workflow { // MULTIQC( // summary, - // fastqc.out, + // FASTQC.out, // ch_multiqc_config // ) } From 2edcdd271fa3beb0e73fcfb26d256ddf0cd46b95 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 08:51:23 +0100 Subject: [PATCH 053/344] Change process name --- .../{{cookiecutter.name_noslash}}/conf/base.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config index 2a2322c95d..580f505c1e 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config @@ -44,7 +44,7 @@ process { withLabel:process_long { time = { check_max( 20.h * task.attempt, 'time' ) } } - withName:get_software_versions { + withName:GET_SOFTWARE_VERSIONS { cache = false } From feddeb68aa3655f3c9f736b2df12974b0f2368a8 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 09:35:12 +0100 Subject: [PATCH 054/344] Hard code pipeline name --- .../pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 230a7aa6e0..4d3a6bcd58 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -16,7 +16,7 @@ nextflow.preview.dsl = 2 */ if (params.help) { // TODO nf-core: Update typical command used to run pipeline - def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv -profile docker" + def command = "nextflow run {{ cookiecutter.name }} --input samplesheet.csv -profile docker" log.info Headers.nf_core(workflow, params.monochrome_logs) log.info JSON.params_help("$baseDir/nextflow_schema.json", command) exit 0 @@ -113,4 +113,3 @@ workflow.onComplete { Completion.email(workflow, params, summary, run_name, baseDir, multiqc_report, log) Completion.summary(workflow, params, log) } - From 79622171cca6f321c5a268d9f2af297335f771eb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 09:40:57 +0100 Subject: [PATCH 055/344] Remove comments --- .../lib/JSON.groovy | 17 ++++++----------- 1 file changed, 6 insertions(+), 11 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy index 43bb98882a..de23cab1ac 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy @@ -6,8 +6,8 @@ import groovy.json.JsonSlurper class JSON { /* - * This method tries to read a JSON params file - */ + * This method tries to read a JSON params file + */ private static LinkedHashMap params_get(String path) { def usage = new LinkedHashMap() try { @@ -47,18 +47,14 @@ class JSON { */ def output_map = new LinkedHashMap() - //Lets go deeper + // Lets go deeper usage.each { key, val -> - //println "Group: $key\n" //Gets the Group names - def Map submap = usage."$key".properties //Gets the property object of the group - //println "Parameters:\n" //nested access to the Parameters + def Map submap = usage."$key".properties // Gets the property object of the group def sub_params = new LinkedHashMap() submap.each { innerkey, value -> - //println "$innerkey\t$value.description" sub_params.put("$innerkey", "$value.description") } output_map.put("$key", sub_params) - //println "\n" } return output_map } @@ -73,11 +69,11 @@ class JSON { String output = "" for (group in usage.keySet()) { output += group + "\n" - def params = usage.get(group) //This gets the parameters of that particular group + def params = usage.get(group) // This gets the parameters of that particular group for (par in params.keySet()) { output+= " \u001B[1m" + par.padRight(27) + "\u001B[1m" + params.get(par) + "\n" } - output += "\n" //Extra newline after parameter block + output += "\n" } return output } @@ -114,4 +110,3 @@ class JSON { return summary } } - From 317c95c777f0dd761551215aa589359a702ea776 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 10:02:48 +0100 Subject: [PATCH 056/344] Rename JSON to Schema --- .../lib/{JSON.groovy => Schema.groovy} | 0 .../pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/{JSON.groovy => Schema.groovy} (100%) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy similarity index 100% rename from nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/JSON.groovy rename to nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 4d3a6bcd58..5b815e1a37 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -18,7 +18,7 @@ if (params.help) { // TODO nf-core: Update typical command used to run pipeline def command = "nextflow run {{ cookiecutter.name }} --input samplesheet.csv -profile docker" log.info Headers.nf_core(workflow, params.monochrome_logs) - log.info JSON.params_help("$baseDir/nextflow_schema.json", command) + log.info Schema.params_help("$baseDir/nextflow_schema.json", command) exit 0 } @@ -62,7 +62,7 @@ run_name = params.name if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { run_name = workflow.runName } -summary = JSON.params_summary(workflow, params, run_name) +summary = Schema.params_summary(workflow, params, run_name) log.info Headers.nf_core(workflow, params.monochrome_logs) log.info summary.collect { k,v -> "${k.padRight(20)}: $v" }.join("\n") log.info "-\033[2m----------------------------------------------------\033[0m-" From fda4e5af2415f8207e9b0585376c1a020aecd854 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 11:16:27 +0200 Subject: [PATCH 057/344] make multiqc process work --- .../{{cookiecutter.name_noslash}}/main.nf | 13 +++++++------ .../modules/local/get_software_versions.nf | 4 ++-- .../modules/nf-core/multiqc.nf | 9 ++++----- 3 files changed, 13 insertions(+), 13 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index fea7f1f5da..ce0222af98 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -23,7 +23,7 @@ if (params.help) { /* * Stage config files */ -ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_config = Channel.fromPath("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true) @@ -84,11 +84,12 @@ workflow { GET_SOFTWARE_VERSIONS() - // MULTIQC( - // summary, - // fastqc.out, - // ch_multiqc_config - // ) + MULTIQC( + ch_multiqc_config.collect(), + ch_multiqc_custom_config.collect().ifEmpty([]), + FASTQC.out.collect(), + GET_SOFTWARE_VERSIONS.out.software_versions_yml.collect()) + ) } /* diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf index 1acadf8ad7..289234b0b2 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf @@ -9,8 +9,8 @@ process GET_SOFTWARE_VERSIONS { } output: - path 'software_versions_mqc.yaml' - path "software_versions.csv" + path 'software_versions_mqc.yaml', emit: software_versions_yml + path "software_versions.csv", emit: software_versions_csv script: // TODO nf-core: Get all tools to print their version number here diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index 7a44eae11a..0283ab7b65 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -28,12 +28,11 @@ process MULTIQC { publishDir "${params.outdir}/multiqc", mode: params.publish_dir_mode input: - path (multiqc_config) from ch_multiqc_config - path (mqc_custom_config) from ch_multiqc_custom_config.collect().ifEmpty([]) + path (multiqc_config) + path (mqc_custom_config) // TODO nf-core: Add in log files from your new processes for MultiQC to find! - path ('fastqc/*') from ch_fastqc_results.collect().ifEmpty([]) - path ('software_versions/*') from ch_software_versions_yaml.collect() - path workflow_summary from ch_workflow_summary.collectFile(name: "workflow_summary_mqc.yaml") + path (fastqc) + path (software_versions) output: path "*multiqc_report.html" From cf5ce26d4ceae97a5a23c592f099897bb69bdaec Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 11:17:19 +0200 Subject: [PATCH 058/344] multiqc process add example modules --- .../{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index 0283ab7b65..c050277e17 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -45,6 +45,6 @@ process MULTIQC { custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ - multiqc -f $rtitle $rfilename $custom_config_file . + multiqc -f $rtitle $rfilename $custom_config_file -m fastqc -m custom_content . """ } From 912eb3d3ce2e89e24e91bdcda3942a2dc134078f Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 11:31:08 +0200 Subject: [PATCH 059/344] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 03cda30cf5..5f0a601476 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -56,6 +56,7 @@ GitHub or on the nf-core [`#json-schema` Slack channel](https://nfcore.slack.com * Fix `markdown_to_html.py` to work with Python 2 and 3. * Change `params.reads` -> `params.input` * Change `params.readPaths` -> `params.input_paths` +* Adding TODOs and MultiQC process in DSL2 template ### Linting From 48c87abf4bdff373133b66c4c27d5a00e598511a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 14:57:39 +0100 Subject: [PATCH 060/344] Add type to help --- .../lib/Schema.groovy | 60 ++++++++++++------- 1 file changed, 37 insertions(+), 23 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy index de23cab1ac..7d0c11e345 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy @@ -4,19 +4,19 @@ import groovy.json.JsonSlurper -class JSON { +class Schema { /* * This method tries to read a JSON params file */ private static LinkedHashMap params_get(String path) { - def usage = new LinkedHashMap() + def params_map = new LinkedHashMap() try { - usage = params_try(path) + params_map = params_try(path) } catch (Exception e) { println "Could not read parameters settings from JSON. $e" - usage = new LinkedHashMap() + params_map = new LinkedHashMap() } - return usage + return params_map } /* @@ -31,7 +31,7 @@ class JSON { private static LinkedHashMap params_try(String path) throws Exception { def json = new File(path).text - def Map usage = (Map) new JsonSlurper().parseText(json).get('properties') + def Map json_params = (Map) new JsonSlurper().parseText(json).get('properties') /* Tree looks like this in nf-core schema * properties <- this is what the first get('properties') gets us @@ -45,39 +45,53 @@ class JSON { properties description */ - def output_map = new LinkedHashMap() - - // Lets go deeper - usage.each { key, val -> - def Map submap = usage."$key".properties // Gets the property object of the group + def params_map = new LinkedHashMap() + json_params.each { key, val -> + def Map group = json_params."$key".properties // Gets the property object of the group def sub_params = new LinkedHashMap() - submap.each { innerkey, value -> - sub_params.put("$innerkey", "$value.description") + group.each { innerkey, value -> + sub_params.put("$innerkey", [ "$value.type", "$value.description" ]) } - output_map.put("$key", sub_params) + params_map.put("$key", sub_params) } - return output_map + return params_map } - static String params_help(path, command) { - String output = "Typical pipeline command:\n\n" - output += " ${command}\n\n" - output += params_beautify(params_get(path)) + private static Integer params_max_chars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def params = params_map.get(group) // This gets the parameters of that particular group + for (par in params.keySet()) { + if (par.size() > max_chars) { + max_chars = par.size() + } + } + } + return max_chars } - static String params_beautify(usage) { + private static String params_beautify(params_map) { String output = "" - for (group in usage.keySet()) { + def max_chars = params_max_chars(params_map) + 1 + for (group in params_map.keySet()) { output += group + "\n" - def params = usage.get(group) // This gets the parameters of that particular group + def params = params_map.get(group) // This gets the parameters of that particular group for (par in params.keySet()) { - output+= " \u001B[1m" + par.padRight(27) + "\u001B[1m" + params.get(par) + "\n" + def type = params.get(par)[0] + def description = params.get(par)[1] + output+= " \u001B[1m" + par.padRight(max_chars) + "\u001B[1m" + type.padRight(9) + description + "\n" } output += "\n" } return output } + private static String params_help(path, command) { + String output = "Typical pipeline command:\n\n" + output += " ${command}\n\n" + output += params_beautify(params_get(path)) + } + private static LinkedHashMap params_summary(workflow, params, run_name) { def Map summary = [:] if (workflow.revision) summary['Pipeline Release'] = workflow.revision From 4144084711e3a8b90c9d523b23a85c75d87b40de Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 13 Jul 2020 16:02:52 +0200 Subject: [PATCH 061/344] Code formatting for the new check_samplesheet.py --- .../bin/check_samplesheet.py | 71 ++++++++++--------- 1 file changed, 36 insertions(+), 35 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index bac1790ea4..de33f787c9 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -6,13 +6,14 @@ import errno import argparse + def parse_args(args=None): - Description = 'Reformat {{ cookiecutter.name }} samplesheet file and check its contents.' + Description = "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." Epilog = """Example usage: python check_samplesheet.py """ parser = argparse.ArgumentParser(description=Description, epilog=Epilog) - parser.add_argument('FILE_IN', help="Input samplesheet file.") - parser.add_argument('FILE_OUT', help="Output file.") + parser.add_argument("FILE_IN", help="Input samplesheet file.") + parser.add_argument("FILE_OUT", help="Output file.") return parser.parse_args(args) @@ -25,67 +26,67 @@ def make_dir(path): raise exception -def print_error(error,line): - print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error,line.strip())) +def print_error(error, line): + print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error, line.strip())) sys.exit(1) -def check_samplesheet(file_in,file_out): +def check_samplesheet(file_in, file_out): sample_run_dict = {} - with open(file_in, 'r') as fin: + with open(file_in, "r") as fin: # TODO nf-core: Update the column names for the input samplesheet ## Check header - HEADER = ['sample', 'fastq_1', 'fastq_2'] - header = fin.readline().strip().split(',') + HEADER = ["sample", "fastq_1", "fastq_2"] + header = fin.readline().strip().split(",") if header != HEADER: - print("ERROR: Please check samplesheet header -> {} != {}".format(','.join(header),','.join(HEADER))) + print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) sys.exit(1) ## Check sample entries for line in fin: - lspl = [x.strip() for x in line.strip().split(',')] + lspl = [x.strip() for x in line.strip().split(",")] ## Check valid number of columns per row if len(lspl) != len(header): - print_error("Invalid number of columns (minimum = {})!".format(len(header)),line) + print_error("Invalid number of columns (minimum = {})!".format(len(header)), line) num_cols = len([x for x in lspl if x]) if num_cols < 2: print_error("Invalid number of populated columns (minimum = 2)!".format(line)) ## Check sample name entries - sample,fastq_files = lspl[0],lspl[1:] + sample, fastq_files = lspl[0], lspl[1:] if sample: - if sample.find(' ') != -1: - print_error("Sample entry contains spaces!",line) + if sample.find(" ") != -1: + print_error("Sample entry contains spaces!", line) else: - print_error("Sample entry has not been specified!",line) + print_error("Sample entry has not been specified!", line) ## Check FastQ file extension for fastq in fastq_files: if fastq: - if fastq.find(' ') != -1: - print_error("FastQ file contains spaces!",line) - if not fastq.endswith('.fastq.gz') and not fastq.endswith('.fq.gz'): - print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",line) + if fastq.find(" ") != -1: + print_error("FastQ file contains spaces!", line) + if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", line) ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2] - fastq_1,fastq_2 = fastq_files - if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ['0', fastq_1, fastq_2] - elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ['1', fastq_1, fastq_2] + sample_info = [] ## [single_end, fastq_1, fastq_2] + fastq_1, fastq_2 = fastq_files + if sample and fastq_1 and fastq_2: ## Paired-end short reads + sample_info = ["0", fastq_1, fastq_2] + elif sample and fastq_1 and not fastq_2: ## Single-end short reads + sample_info = ["1", fastq_1, fastq_2] else: - print_error("Invalid combination of columns provided!",line) + print_error("Invalid combination of columns provided!", line) if sample not in sample_run_dict: sample_run_dict[sample] = [sample_info] else: if sample_info in sample_run_dict[sample]: - print_error("Samplesheet contains duplicate rows!",line) + print_error("Samplesheet contains duplicate rows!", line) else: sample_run_dict[sample].append(sample_info) @@ -93,23 +94,23 @@ def check_samplesheet(file_in,file_out): if len(sample_run_dict) > 0: out_dir = os.path.dirname(file_out) make_dir(out_dir) - fout = open(file_out,'w') - fout.write(','.join(['sample', 'single_end', 'fastq_1', 'fastq_2']) + '\n') + fout = open(file_out, "w") + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") for sample in sorted(sample_run_dict.keys()): ## Check that multiple runs of the same sample are of the same datatype if not all(x[0] == sample_run_dict[sample][0][0] for x in sample_run_dict[sample]): - print_error("Multiple runs of a sample must be of the same datatype","Sample: {}".format(sample)) + print_error("Multiple runs of a sample must be of the same datatype", "Sample: {}".format(sample)) - for idx,val in enumerate(sample_run_dict[sample]): - fout.write(','.join(["{}_T{}".format(sample,idx+1)] + val) + '\n') + for idx, val in enumerate(sample_run_dict[sample]): + fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") fout.close() def main(args=None): args = parse_args(args) - check_samplesheet(args.FILE_IN,args.FILE_OUT) + check_samplesheet(args.FILE_IN, args.FILE_OUT) -if __name__ == '__main__': +if __name__ == "__main__": sys.exit(main()) From 782abb77e919140701def96f4393ee9bd5ffb5d5 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Mon, 13 Jul 2020 16:14:31 +0200 Subject: [PATCH 062/344] Newline for Black --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 1 + 1 file changed, 1 insertion(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 041538015a..97723040ed 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -31,6 +31,7 @@ def print_error(error, line): print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error, line.strip())) sys.exit(1) + # TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in, file_out): """ From 95f030a0303223d95b95a1deb79afd55b7637e62 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 13 Jul 2020 15:15:56 +0100 Subject: [PATCH 063/344] Add braces around param type --- .../{{cookiecutter.name_noslash}}/lib/Schema.groovy | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy index 7d0c11e345..3e91a9ed43 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy @@ -77,9 +77,9 @@ class Schema { output += group + "\n" def params = params_map.get(group) // This gets the parameters of that particular group for (par in params.keySet()) { - def type = params.get(par)[0] + def type = "[" + params.get(par)[0] + "]" def description = params.get(par)[1] - output+= " \u001B[1m" + par.padRight(max_chars) + "\u001B[1m" + type.padRight(9) + description + "\n" + output+= " \u001B[1m" + par.padRight(max_chars) + "\u001B[1m" + type.padRight(10) + description + "\n" } output += "\n" } From 795472438cd401e00ea1a19e329b9ccc122ef3f9 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 21:47:52 +0200 Subject: [PATCH 064/344] multiqc process DSL2 template --- .../lib/Schema.groovy | 16 ++++++++++++++++ .../{{cookiecutter.name_noslash}}/main.nf | 6 +++++- .../modules/nf-core/multiqc.nf | 2 ++ 3 files changed, 23 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy index de23cab1ac..0209b7187a 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy @@ -109,4 +109,20 @@ class JSON { } return summary } + + static String params_mqc_summary(summary) { + String yaml_file_text = """ + id: '{{ cookiecutter.name_noslash }}-summary' + description: " - this information is collected when the pipeline is started." + section_name: '{{ cookiecutter.name }} Workflow Summary' + section_href: 'https://github.com/{{ cookiecutter.name }}' + plot_type: 'html' + data: | +
+ ${summary.collect { k,v -> "
$k
${v ?: 'N/A'}
" }.join("\n")} +
+ """.stripIndent() + + return yaml_file_text + } } diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 3e5c9e7452..991b42afbe 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -67,6 +67,9 @@ log.info Headers.nf_core(workflow, params.monochrome_logs) log.info summary.collect { k,v -> "${k.padRight(20)}: $v" }.join("\n") log.info "-\033[2m----------------------------------------------------\033[0m-" +workflow_summary = Schema.params_multiqc_summary(summary) +ch_workflow_summary = Channel.value(workflow_summary) + /* * Include local pipeline modules */ @@ -102,7 +105,8 @@ workflow { ch_multiqc_config.collect(), ch_multiqc_custom_config.collect().ifEmpty([]), FASTQC.out.collect(), - GET_SOFTWARE_VERSIONS.out.software_versions_yml.collect()) + GET_SOFTWARE_VERSIONS.out.software_versions_yml.collect(), + ch_workflow_summary) ) } diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index c050277e17..694c8a7605 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -33,6 +33,7 @@ process MULTIQC { // TODO nf-core: Add in log files from your new processes for MultiQC to find! path (fastqc) path (software_versions) + val (workflow_summary) output: path "*multiqc_report.html" @@ -45,6 +46,7 @@ process MULTIQC { custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ + echo '$workflow_summary' > workflow_summary_mqc.yaml multiqc -f $rtitle $rfilename $custom_config_file -m fastqc -m custom_content . """ } From deacc0fe51003b4cee4a747eb15a2922c50f9524 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 21:54:46 +0200 Subject: [PATCH 065/344] clean multiqc module --- .../modules/nf-core/multiqc.nf | 16 ---------------- 1 file changed, 16 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index 694c8a7605..7e01bf110b 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -5,22 +5,6 @@ if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { custom_runName = workflow.runName } -// Channel.from(summary.collect{ [it.key, it.value] }) -// .map { k,v -> "
$k
${v ?: 'N/A'}
" } -// .reduce { a, b -> return [a, b].join("\n ") } -// .map { x -> """ -// id: '{{ cookiecutter.name_noslash }}-summary' -// description: " - this information is collected when the pipeline is started." -// section_name: '{{ cookiecutter.name }} Workflow Summary' -// section_href: 'https://github.com/{{ cookiecutter.name }}' -// plot_type: 'html' -// data: | -//
-// $x -//
-// """.stripIndent() } -// .set { ch_workflow_summary } - /* * MultiQC */ From fdd32a36a3d3bda0e142045be33283ff82f268d4 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 22:02:40 +0200 Subject: [PATCH 066/344] DSL2 template fix parenthesis --- nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 991b42afbe..7d15bc6512 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -107,7 +107,6 @@ workflow { FASTQC.out.collect(), GET_SOFTWARE_VERSIONS.out.software_versions_yml.collect(), ch_workflow_summary) - ) } /* From 462513ba9ebb1b95b1fac0a92e60f02d5c20e984 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 22:06:06 +0200 Subject: [PATCH 067/344] fix typo --- nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 7d15bc6512..6e3131f3b8 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -67,7 +67,7 @@ log.info Headers.nf_core(workflow, params.monochrome_logs) log.info summary.collect { k,v -> "${k.padRight(20)}: $v" }.join("\n") log.info "-\033[2m----------------------------------------------------\033[0m-" -workflow_summary = Schema.params_multiqc_summary(summary) +workflow_summary = Schema.params_mqc_summary(summary) ch_workflow_summary = Channel.value(workflow_summary) /* From ae4591403c4bc7d89566d07bd851df69334aa029 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Mon, 13 Jul 2020 22:14:32 +0200 Subject: [PATCH 068/344] fix python lint --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 1 + 1 file changed, 1 insertion(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 041538015a..97723040ed 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -31,6 +31,7 @@ def print_error(error, line): print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error, line.strip())) sys.exit(1) + # TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in, file_out): """ From e270b51ce4b981b8a3a89b5ae3dac44df38ee2cc Mon Sep 17 00:00:00 2001 From: Gisela Gabernet Date: Tue, 14 Jul 2020 08:52:15 +0200 Subject: [PATCH 069/344] apply review comments Co-authored-by: Harshil Patel --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 97723040ed..6f6c2af3cd 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -38,7 +38,7 @@ def check_samplesheet(file_in, file_out): This function checks that the sample sheet follows the following structure: sample,fastq_1,fastq_2 - sample1,Sample1.fastq.gz,Sample2.fastq.gz + SAMPLE1,SAMPLE1_1.fastq.gz,SAMPLE1_2.fastq.gz """ sample_run_dict = {} From ac825cc07a4efd7efd9b3405bdf3d27a529f6f0d Mon Sep 17 00:00:00 2001 From: Gisela Gabernet Date: Tue, 14 Jul 2020 09:07:46 +0200 Subject: [PATCH 070/344] apply review comments Co-authored-by: Harshil Patel --- .../modules/local/get_software_versions.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf index 289234b0b2..eb27334619 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf @@ -9,7 +9,7 @@ process GET_SOFTWARE_VERSIONS { } output: - path 'software_versions_mqc.yaml', emit: software_versions_yml + path 'software_versions_mqc.yaml', emit: yml path "software_versions.csv", emit: software_versions_csv script: From 57c04e494feab7be356575f65afa296e3401f5a4 Mon Sep 17 00:00:00 2001 From: Gisela Gabernet Date: Tue, 14 Jul 2020 09:08:05 +0200 Subject: [PATCH 071/344] apply review comments Co-authored-by: Harshil Patel --- nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 6e3131f3b8..833fe98353 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -105,7 +105,7 @@ workflow { ch_multiqc_config.collect(), ch_multiqc_custom_config.collect().ifEmpty([]), FASTQC.out.collect(), - GET_SOFTWARE_VERSIONS.out.software_versions_yml.collect(), + GET_SOFTWARE_VERSIONS.out.yml.collect(), ch_workflow_summary) } From 30bc59f50726ad647e8f66dabf63eb981f99f727 Mon Sep 17 00:00:00 2001 From: Gisela Gabernet Date: Tue, 14 Jul 2020 09:09:02 +0200 Subject: [PATCH 072/344] apply review comments Co-authored-by: Harshil Patel --- .../modules/local/get_software_versions.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf index eb27334619..b583603404 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf @@ -10,7 +10,7 @@ process GET_SOFTWARE_VERSIONS { output: path 'software_versions_mqc.yaml', emit: yml - path "software_versions.csv", emit: software_versions_csv + path "software_versions.csv", emit: csv script: // TODO nf-core: Get all tools to print their version number here From 2a22b978e9f30b58011e6560cb14307533c1cdfa Mon Sep 17 00:00:00 2001 From: Gisela Gabernet Date: Tue, 14 Jul 2020 09:10:25 +0200 Subject: [PATCH 073/344] apply review comments Co-authored-by: Harshil Patel --- .../{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index 7e01bf110b..aeec597a30 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -31,6 +31,6 @@ process MULTIQC { // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time """ echo '$workflow_summary' > workflow_summary_mqc.yaml - multiqc -f $rtitle $rfilename $custom_config_file -m fastqc -m custom_content . + multiqc -f $rtitle $rfilename $custom_config_file . """ } From aa040b014d7bc1a578b0e34ccdf0ea198f7d2a87 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Tue, 14 Jul 2020 09:21:07 +0200 Subject: [PATCH 074/344] remove braces mutiqc process --- .../modules/nf-core/multiqc.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf index 7e01bf110b..14f1e16575 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf @@ -12,12 +12,12 @@ process MULTIQC { publishDir "${params.outdir}/multiqc", mode: params.publish_dir_mode input: - path (multiqc_config) - path (mqc_custom_config) + path multiqc_config + path mqc_custom_config // TODO nf-core: Add in log files from your new processes for MultiQC to find! - path (fastqc) - path (software_versions) - val (workflow_summary) + path fastqc + path software_versions + val workflow_summary output: path "*multiqc_report.html" From 5301a1dfd72bd1e80822c8c1002f158c85efb7a4 Mon Sep 17 00:00:00 2001 From: ggabernet Date: Tue, 14 Jul 2020 10:05:57 +0200 Subject: [PATCH 075/344] implement review comments --- .../{{cookiecutter.name_noslash}}/lib/Completion.groovy | 2 +- .../pipeline-template/{{cookiecutter.name_noslash}}/main.nf | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy index 0a7a2b555d..e709701333 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy @@ -111,7 +111,7 @@ class Completion { if (workflow.success) { log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" } else { - Checks.hostname() + Checks.hostname(workflow, params, log) log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" } } diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 833fe98353..239889c3de 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -25,7 +25,7 @@ if (params.help) { /* * Stage config files */ -ch_multiqc_config = Channel.fromPath("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() ch_output_docs = file("$baseDir/docs/output.md", checkIfExists: true) ch_output_docs_images = file("$baseDir/docs/images/", checkIfExists: true) @@ -102,7 +102,7 @@ workflow { GET_SOFTWARE_VERSIONS() MULTIQC( - ch_multiqc_config.collect(), + ch_multiqc_config, ch_multiqc_custom_config.collect().ifEmpty([]), FASTQC.out.collect(), GET_SOFTWARE_VERSIONS.out.yml.collect(), From cf3f372ecd361d0f9e704999c5c3c148c0dabefd Mon Sep 17 00:00:00 2001 From: drpatelh Date: Fri, 24 Jul 2020 15:53:48 +0100 Subject: [PATCH 076/344] Use base expansion for genome indices --- .../conf/igenomes.config | 149 +++++++++--------- 1 file changed, 74 insertions(+), 75 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config index caeafceb25..8fb4b874e9 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config @@ -12,8 +12,8 @@ params { genomes { 'GRCh37' { fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" @@ -25,8 +25,8 @@ params { } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" @@ -37,8 +37,8 @@ params { } 'GRCm38' { fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" @@ -50,8 +50,8 @@ params { } 'TAIR10' { fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" @@ -61,8 +61,8 @@ params { } 'EB2' { fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" @@ -71,8 +71,8 @@ params { } 'UMD3.1' { fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" @@ -82,8 +82,8 @@ params { } 'WBcel235' { fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" @@ -93,8 +93,8 @@ params { } 'CanFam3.1' { fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" @@ -104,8 +104,8 @@ params { } 'GRCz10' { fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" @@ -114,8 +114,8 @@ params { } 'BDGP6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" @@ -125,8 +125,8 @@ params { } 'EquCab2' { fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" @@ -136,8 +136,8 @@ params { } 'EB1' { fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" @@ -146,8 +146,8 @@ params { } 'Galgal4' { fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" @@ -156,8 +156,8 @@ params { } 'Gm01' { fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" @@ -166,8 +166,8 @@ params { } 'Mmul_1' { fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" @@ -177,8 +177,8 @@ params { } 'IRGSP-1.0' { fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" @@ -187,8 +187,8 @@ params { } 'CHIMP2.1.4' { fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" @@ -198,8 +198,8 @@ params { } 'Rnor_6.0' { fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" @@ -208,8 +208,8 @@ params { } 'R64-1-1' { fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" @@ -219,8 +219,8 @@ params { } 'EF2' { fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" @@ -231,8 +231,8 @@ params { } 'Sbi1' { fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" @@ -241,8 +241,8 @@ params { } 'Sscrofa10.2' { fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" @@ -252,8 +252,8 @@ params { } 'AGPv3' { fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" @@ -262,8 +262,8 @@ params { } 'hg38' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" @@ -274,8 +274,8 @@ params { } 'hg19' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" @@ -287,8 +287,8 @@ params { } 'mm10' { fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" @@ -300,8 +300,8 @@ params { } 'bosTau8' { fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" @@ -310,8 +310,8 @@ params { } 'ce10' { fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" @@ -322,8 +322,8 @@ params { } 'canFam3' { fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" @@ -333,19 +333,18 @@ params { } 'danRer10' { fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" mito_name = "chrM" - macs_gsize = "1.37e9" } 'dm6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" @@ -355,8 +354,8 @@ params { } 'equCab2' { fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" @@ -366,8 +365,8 @@ params { } 'galGal4' { fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" @@ -377,8 +376,8 @@ params { } 'panTro4' { fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" @@ -388,8 +387,8 @@ params { } 'rn6' { fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" @@ -398,8 +397,8 @@ params { } 'sacCer3' { fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" @@ -408,8 +407,8 @@ params { } 'susScr3' { fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" From 5dd6aabfeb7d8d39ccc1cd5e324fc6a1bf041baf Mon Sep 17 00:00:00 2001 From: drpatelh Date: Fri, 24 Jul 2020 15:56:29 +0100 Subject: [PATCH 077/344] Update CHANGELOG --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 14f67a2c81..2cd86892d6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -64,6 +64,7 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Fix `markdown_to_html.py` to work with Python 2 and 3. * Change `params.reads` -> `params.input` * Change `params.readPaths` -> `params.input_paths` +* [#522](https://github.com/nf-core/tools/issues/522) - Unify iGenomes index usage ### Linting From ea000ac7b6b94258448bf78ad92276840eac82b7 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 16:31:18 +0000 Subject: [PATCH 078/344] Update CHANGELOG --- CHANGELOG.md | 1 - 1 file changed, 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ee42ba15ac..e8173fb38b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -155,7 +155,6 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Adding TODOs and MultiQC process in DSL2 template * Change `params.readPaths` -> `params.input_paths` * Added a `.github/.dockstore.yml` config file for automatic workflow registration with [dockstore.org](https://dockstore.org/) ->>>>>>> 9817bce2698c0cde70d159434259686a1519fec2 ### Linting From 02b6a9658c1b97abd268e65463d3eb31664795ed Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 16:46:13 +0000 Subject: [PATCH 079/344] Update samplesheet --- .../bin/check_samplesheet.py | 100 +++++++++++------- 1 file changed, 62 insertions(+), 38 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 6f6c2af3cd..a8b46a5ae5 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -1,4 +1,5 @@ #!/usr/bin/env python + # TODO nf-core: Update the script to check the samplesheet # This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_sispa.csv @@ -10,7 +11,7 @@ def parse_args(args=None): Description = "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." - Epilog = """Example usage: python check_samplesheet.py """ + Epilog = "Example usage: python check_samplesheet.py " parser = argparse.ArgumentParser(description=Description, epilog=Epilog) parser.add_argument("FILE_IN", help="Input samplesheet file.") @@ -27,92 +28,115 @@ def make_dir(path): raise exception -def print_error(error, line): - print("ERROR: Please check samplesheet -> {}\nLine: '{}'".format(error, line.strip())) +def print_error(error, context='Line', context_str=''): + error_str = "ERROR: Please check samplesheet -> {}".format(error) + if context != '' and context_str != '': + error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(error, context.strip(), context_str.strip()) + print(error_str) sys.exit(1) # TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in, file_out): """ - This function checks that the sample sheet follows the following structure: - - sample,fastq_1,fastq_2 - SAMPLE1,SAMPLE1_1.fastq.gz,SAMPLE1_2.fastq.gz + This function checks that the samplesheet follows the following structure: + + group,replicate,fastq_1,fastq_2 + WT,1,WT_LIB1_REP1_1.fastq.gz,WT_LIB1_REP1_2.fastq.gz + WT,1,WT_LIB2_REP1_1.fastq.gz,WT_LIB2_REP1_2.fastq.gz + WT,2,WT_LIB1_REP2_1.fastq.gz,WT_LIB1_REP2_2.fastq.gz + KO,1,KO_LIB1_REP1_1.fastq.gz,KO_LIB1_REP1_2.fastq.gz """ sample_run_dict = {} with open(file_in, "r") as fin: - # TODO nf-core: Update the column names for the input samplesheet ## Check header - HEADER = ["sample", "fastq_1", "fastq_2"] - header = fin.readline().strip().split(",") - if header != HEADER: + MIN_COLS = 3 + # TODO nf-core: Update the column names for the input samplesheet + HEADER = ['group', 'replicate', 'fastq_1', 'fastq_2'] + header = [x.strip('"') for x in fin.readline().strip().split(",")] + if header[:len(HEADER)] != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) sys.exit(1) ## Check sample entries for line in fin: - lspl = [x.strip() for x in line.strip().split(",")] + lspl = [x.strip().strip('"') for x in line.strip().split(",")] ## Check valid number of columns per row - if len(lspl) != len(header): - print_error("Invalid number of columns (minimum = {})!".format(len(header)), line) + if len(lspl) < len(HEADER): + print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), 'Line', line) num_cols = len([x for x in lspl if x]) - if num_cols < 2: - print_error("Invalid number of populated columns (minimum = 2)!".format(line)) + if num_cols < MIN_COLS: + print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), 'Line', line) ## Check sample name entries - sample, fastq_files = lspl[0], lspl[1:] + sample, replicate, fastq_1, fastq_2 = lspl[:len(HEADER)] if sample: if sample.find(" ") != -1: - print_error("Sample entry contains spaces!", line) + print_error("Group entry contains spaces!", 'Line', line) else: - print_error("Sample entry has not been specified!", line) + print_error("Group entry has not been specified!", 'Line', line) + + ## Check replicate entry is integer + if not replicate.isdigit(): + print_error("Replicate id not an integer!", 'Line', line) + replicate = int(replicate) ## Check FastQ file extension - for fastq in fastq_files: + for fastq in [fastq_1, fastq_2]: if fastq: if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", line) + print_error("FastQ file contains spaces!", 'Line', line) if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): - print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", line) + print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", 'Line', line) ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2] - fastq_1, fastq_2 = fastq_files if sample and fastq_1 and fastq_2: ## Paired-end short reads sample_info = ["0", fastq_1, fastq_2] elif sample and fastq_1 and not fastq_2: ## Single-end short reads sample_info = ["1", fastq_1, fastq_2] else: - print_error("Invalid combination of columns provided!", line) + print_error("Invalid combination of columns provided!", 'Line', line) + ## Create sample mapping dictionary = {sample: {replicate : [ single_end, fastq_1, fastq_2 ]}} if sample not in sample_run_dict: - sample_run_dict[sample] = [sample_info] + sample_run_dict[sample] = {} + if replicate not in sample_run_dict[sample]: + sample_run_dict[sample][replicate] = [sample_info] else: - if sample_info in sample_run_dict[sample]: - print_error("Samplesheet contains duplicate rows!", line) + if sample_info in sample_run_dict[sample][replicate]: + print_error("Samplesheet contains duplicate rows!", 'Line', line) else: - sample_run_dict[sample].append(sample_info) + sample_run_dict[sample][replicate].append(sample_info) ## Write validated samplesheet with appropriate columns if len(sample_run_dict) > 0: out_dir = os.path.dirname(file_out) make_dir(out_dir) - fout = open(file_out, "w") - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") - for sample in sorted(sample_run_dict.keys()): + with open(file_out, "w") as fout: + + fout.write(",".join(['sample', 'single_end', 'fastq_1', 'fastq_2']) + "\n") + for sample in sorted(sample_run_dict.keys()): + + ## Check that replicate ids are in format 1.. + uniq_rep_ids = set(sample_run_dict[sample].keys()) + if len(uniq_rep_ids) != max(uniq_rep_ids): + print_error("Replicate ids must start with 1..!", 'Group', sample) + + for replicate in sorted(sample_run_dict[sample].keys()): - ## Check that multiple runs of the same sample are of the same datatype - if not all(x[0] == sample_run_dict[sample][0][0] for x in sample_run_dict[sample]): - print_error("Multiple runs of a sample must be of the same datatype", "Sample: {}".format(sample)) + ## Check that multiple runs of the same sample are of the same datatype + if not all(x[0] == sample_run_dict[sample][replicate][0][0] for x in sample_run_dict[sample][replicate]): + print_error("Multiple runs of a sample must be of the same datatype!", 'Group', sample) - for idx, val in enumerate(sample_run_dict[sample]): - fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") - fout.close() + ## Write to file + for idx, sample_info in enumerate(sample_run_dict[sample][replicate]): + sample_id = "{}_R{}_T{}".format(sample,replicate,idx+1) + fout.write(','.join([sample_id] + sample_info) + '\n') def main(args=None): @@ -121,4 +145,4 @@ def main(args=None): if __name__ == "__main__": - sys.exit(main()) + sys.exit(main()) \ No newline at end of file From 7dd4f9b1b343e2b40b5405e4a04db2aced1dac59 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 16:50:47 +0000 Subject: [PATCH 080/344] Use Black formatting --- .../bin/check_samplesheet.py | 94 ++++++++++++------- 1 file changed, 60 insertions(+), 34 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index a8b46a5ae5..688f04f568 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -10,7 +10,9 @@ def parse_args(args=None): - Description = "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." + Description = ( + "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." + ) Epilog = "Example usage: python check_samplesheet.py " parser = argparse.ArgumentParser(description=Description, epilog=Epilog) @@ -28,10 +30,12 @@ def make_dir(path): raise exception -def print_error(error, context='Line', context_str=''): +def print_error(error, context="Line", context_str=""): error_str = "ERROR: Please check samplesheet -> {}".format(error) - if context != '' and context_str != '': - error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format(error, context.strip(), context_str.strip()) + if context != "" and context_str != "": + error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( + error, context.strip(), context_str.strip() + ) print(error_str) sys.exit(1) @@ -54,45 +58,58 @@ def check_samplesheet(file_in, file_out): ## Check header MIN_COLS = 3 # TODO nf-core: Update the column names for the input samplesheet - HEADER = ['group', 'replicate', 'fastq_1', 'fastq_2'] + HEADER = ["group", "replicate", "fastq_1", "fastq_2"] header = [x.strip('"') for x in fin.readline().strip().split(",")] - if header[:len(HEADER)] != HEADER: - print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) + if header[: len(HEADER)] != HEADER: + print( + "ERROR: Please check samplesheet header -> {} != {}".format( + ",".join(header), ",".join(HEADER) + ) + ) sys.exit(1) - ## Check sample entries for line in fin: lspl = [x.strip().strip('"') for x in line.strip().split(",")] ## Check valid number of columns per row if len(lspl) < len(HEADER): - print_error("Invalid number of columns (minimum = {})!".format(len(HEADER)), 'Line', line) - + print_error( + "Invalid number of columns (minimum = {})!".format(len(HEADER)), + "Line", + line, + ) num_cols = len([x for x in lspl if x]) if num_cols < MIN_COLS: - print_error("Invalid number of populated columns (minimum = {})!".format(MIN_COLS), 'Line', line) - + print_error( + "Invalid number of populated columns (minimum = {})!".format( + MIN_COLS + ), + "Line", + line, + ) ## Check sample name entries - sample, replicate, fastq_1, fastq_2 = lspl[:len(HEADER)] + sample, replicate, fastq_1, fastq_2 = lspl[: len(HEADER)] if sample: if sample.find(" ") != -1: - print_error("Group entry contains spaces!", 'Line', line) + print_error("Group entry contains spaces!", "Line", line) else: - print_error("Group entry has not been specified!", 'Line', line) - + print_error("Group entry has not been specified!", "Line", line) ## Check replicate entry is integer if not replicate.isdigit(): - print_error("Replicate id not an integer!", 'Line', line) + print_error("Replicate id not an integer!", "Line", line) replicate = int(replicate) ## Check FastQ file extension for fastq in [fastq_1, fastq_2]: if fastq: if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", 'Line', line) + print_error("FastQ file contains spaces!", "Line", line) if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): - print_error("FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", 'Line', line) - + print_error( + "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", + "Line", + line, + ) ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2] if sample and fastq_1 and fastq_2: ## Paired-end short reads @@ -100,8 +117,7 @@ def check_samplesheet(file_in, file_out): elif sample and fastq_1 and not fastq_2: ## Single-end short reads sample_info = ["1", fastq_1, fastq_2] else: - print_error("Invalid combination of columns provided!", 'Line', line) - + print_error("Invalid combination of columns provided!", "Line", line) ## Create sample mapping dictionary = {sample: {replicate : [ single_end, fastq_1, fastq_2 ]}} if sample not in sample_run_dict: sample_run_dict[sample] = {} @@ -109,34 +125,44 @@ def check_samplesheet(file_in, file_out): sample_run_dict[sample][replicate] = [sample_info] else: if sample_info in sample_run_dict[sample][replicate]: - print_error("Samplesheet contains duplicate rows!", 'Line', line) + print_error("Samplesheet contains duplicate rows!", "Line", line) else: sample_run_dict[sample][replicate].append(sample_info) - ## Write validated samplesheet with appropriate columns if len(sample_run_dict) > 0: out_dir = os.path.dirname(file_out) make_dir(out_dir) with open(file_out, "w") as fout: - fout.write(",".join(['sample', 'single_end', 'fastq_1', 'fastq_2']) + "\n") + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") for sample in sorted(sample_run_dict.keys()): ## Check that replicate ids are in format 1.. uniq_rep_ids = set(sample_run_dict[sample].keys()) if len(uniq_rep_ids) != max(uniq_rep_ids): - print_error("Replicate ids must start with 1..!", 'Group', sample) - + print_error( + "Replicate ids must start with 1..!", + "Group", + sample, + ) for replicate in sorted(sample_run_dict[sample].keys()): ## Check that multiple runs of the same sample are of the same datatype - if not all(x[0] == sample_run_dict[sample][replicate][0][0] for x in sample_run_dict[sample][replicate]): - print_error("Multiple runs of a sample must be of the same datatype!", 'Group', sample) - + if not all( + x[0] == sample_run_dict[sample][replicate][0][0] + for x in sample_run_dict[sample][replicate] + ): + print_error( + "Multiple runs of a sample must be of the same datatype!", + "Group", + sample, + ) ## Write to file - for idx, sample_info in enumerate(sample_run_dict[sample][replicate]): - sample_id = "{}_R{}_T{}".format(sample,replicate,idx+1) - fout.write(','.join([sample_id] + sample_info) + '\n') + for idx, sample_info in enumerate( + sample_run_dict[sample][replicate] + ): + sample_id = "{}_R{}_T{}".format(sample, replicate, idx + 1) + fout.write(",".join([sample_id] + sample_info) + "\n") def main(args=None): @@ -145,4 +171,4 @@ def main(args=None): if __name__ == "__main__": - sys.exit(main()) \ No newline at end of file + sys.exit(main()) From a0d3fe64b62559007e1453ae385b0a07855b6e14 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:18:31 +0000 Subject: [PATCH 081/344] Fix Black linting --- .../bin/check_samplesheet.py | 21 +++++-------------- 1 file changed, 5 insertions(+), 16 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 688f04f568..49533deb84 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -10,9 +10,7 @@ def parse_args(args=None): - Description = ( - "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." - ) + Description = "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." Epilog = "Example usage: python check_samplesheet.py " parser = argparse.ArgumentParser(description=Description, epilog=Epilog) @@ -61,11 +59,7 @@ def check_samplesheet(file_in, file_out): HEADER = ["group", "replicate", "fastq_1", "fastq_2"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: - print( - "ERROR: Please check samplesheet header -> {} != {}".format( - ",".join(header), ",".join(HEADER) - ) - ) + print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) sys.exit(1) ## Check sample entries for line in fin: @@ -81,9 +75,7 @@ def check_samplesheet(file_in, file_out): num_cols = len([x for x in lspl if x]) if num_cols < MIN_COLS: print_error( - "Invalid number of populated columns (minimum = {})!".format( - MIN_COLS - ), + "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line, ) @@ -149,8 +141,7 @@ def check_samplesheet(file_in, file_out): ## Check that multiple runs of the same sample are of the same datatype if not all( - x[0] == sample_run_dict[sample][replicate][0][0] - for x in sample_run_dict[sample][replicate] + x[0] == sample_run_dict[sample][replicate][0][0] for x in sample_run_dict[sample][replicate] ): print_error( "Multiple runs of a sample must be of the same datatype!", @@ -158,9 +149,7 @@ def check_samplesheet(file_in, file_out): sample, ) ## Write to file - for idx, sample_info in enumerate( - sample_run_dict[sample][replicate] - ): + for idx, sample_info in enumerate(sample_run_dict[sample][replicate]): sample_id = "{}_R{}_T{}".format(sample, replicate, idx + 1) fout.write(",".join([sample_id] + sample_info) + "\n") From e56190bf9fe275ce9a9da63e548a5eaa7818d281 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:20:08 +0000 Subject: [PATCH 082/344] Fix with Black --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index 49533deb84..aa25cde165 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -61,6 +61,7 @@ def check_samplesheet(file_in, file_out): if header[: len(HEADER)] != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) sys.exit(1) + ## Check sample entries for line in fin: lspl = [x.strip().strip('"') for x in line.strip().split(",")] @@ -79,6 +80,7 @@ def check_samplesheet(file_in, file_out): "Line", line, ) + ## Check sample name entries sample, replicate, fastq_1, fastq_2 = lspl[: len(HEADER)] if sample: @@ -86,6 +88,7 @@ def check_samplesheet(file_in, file_out): print_error("Group entry contains spaces!", "Line", line) else: print_error("Group entry has not been specified!", "Line", line) + ## Check replicate entry is integer if not replicate.isdigit(): print_error("Replicate id not an integer!", "Line", line) @@ -102,6 +105,7 @@ def check_samplesheet(file_in, file_out): "Line", line, ) + ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2] if sample and fastq_1 and fastq_2: ## Paired-end short reads @@ -120,6 +124,7 @@ def check_samplesheet(file_in, file_out): print_error("Samplesheet contains duplicate rows!", "Line", line) else: sample_run_dict[sample][replicate].append(sample_info) + ## Write validated samplesheet with appropriate columns if len(sample_run_dict) > 0: out_dir = os.path.dirname(file_out) From 316df0152598fc8c321e6b18579946ae669ca7cd Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:23:19 +0000 Subject: [PATCH 083/344] Update software scraping --- .../bin/scrape_software_versions.py | 56 ++++++------------- 1 file changed, 18 insertions(+), 38 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py index a6d687ce1b..3a767bab21 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py @@ -1,40 +1,21 @@ #!/usr/bin/env python from __future__ import print_function -from collections import OrderedDict -import re +import os -# TODO nf-core: Add additional regexes for new tools in process get_software_versions -regexes = { - "{{ cookiecutter.name }}": ["v_pipeline.txt", r"(\S+)"], - "Nextflow": ["v_nextflow.txt", r"(\S+)"], - "FastQC": ["v_fastqc.txt", r"FastQC v(\S+)"], - "MultiQC": ["v_multiqc.txt", r"multiqc, version (\S+)"], -} -results = OrderedDict() -results["{{ cookiecutter.name }}"] = 'N/A' -results["Nextflow"] = 'N/A' -results["FastQC"] = 'N/A' -results["MultiQC"] = 'N/A' +results = {} +version_files = [x for x in os.listdir('.') if x.endswith('.version.txt')] +for version_file in version_files: -# Search each file using its regex -for k, v in regexes.items(): - try: - with open(v[0]) as x: - versions = x.read() - match = re.search(v[1], versions) - if match: - results[k] = "v{}".format(match.group(1)) - except IOError: - results[k] = False + software = version_file.replace('.version.txt','') + if software == 'pipeline': + software = '{{ cookiecutter.name }}' -# Remove software set to false in results -for k in list(results): - if not results[k]: - del results[k] + with open(version_file) as fin: + version = fin.read().strip() + results[software] = version # Dump to YAML -print( - """ +print (''' id: 'software_versions' section_name: '{{ cookiecutter.name }} Software Versions' section_href: 'https://github.com/{{ cookiecutter.name }}' @@ -42,13 +23,12 @@ description: 'are collected at run time from the software output.' data: |
-""" -) -for k, v in results.items(): - print("
{}
{}
".format(k, v)) -print("
") +''') +for k,v in sorted(results.items()): + print("
{}
{}
".format(k,v)) +print (" ") # Write out regexes as csv file: -with open("software_versions.csv", "w") as f: - for k, v in results.items(): - f.write("{}\t{}\n".format(k, v)) +with open('software_versions.csv', 'w') as f: + for k,v in sorted(results.items()): + f.write("{}\t{}\n".format(k,v)) \ No newline at end of file From 00699e533fe7261aaf336b7fbd6f561f1eb2103b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:25:02 +0000 Subject: [PATCH 084/344] Retire markdown_to_html.py script --- .../bin/markdown_to_html.py | 91 ------------------- 1 file changed, 91 deletions(-) delete mode 100755 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/markdown_to_html.py diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/markdown_to_html.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/markdown_to_html.py deleted file mode 100755 index a26d1ff5e6..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/markdown_to_html.py +++ /dev/null @@ -1,91 +0,0 @@ -#!/usr/bin/env python -from __future__ import print_function -import argparse -import markdown -import os -import sys -import io - - -def convert_markdown(in_fn): - input_md = io.open(in_fn, mode="r", encoding="utf-8").read() - html = markdown.markdown( - "[TOC]\n" + input_md, - extensions=["pymdownx.extra", "pymdownx.b64", "pymdownx.highlight", "pymdownx.emoji", "pymdownx.tilde", "toc"], - extension_configs={ - "pymdownx.b64": {"base_path": os.path.dirname(in_fn)}, - "pymdownx.highlight": {"noclasses": True}, - "toc": {"title": "Table of Contents"}, - }, - ) - return html - - -def wrap_html(contents): - header = """ - - - - - -
- """ - footer = """ -
- - - """ - return header + contents + footer - - -def parse_args(args=None): - parser = argparse.ArgumentParser() - parser.add_argument("mdfile", type=argparse.FileType("r"), nargs="?", help="File to convert. Defaults to stdin.") - parser.add_argument( - "-o", "--out", type=argparse.FileType("w"), default=sys.stdout, help="Output file name. Defaults to stdout." - ) - return parser.parse_args(args) - - -def main(args=None): - args = parse_args(args) - converted_md = convert_markdown(args.mdfile.name) - html = wrap_html(converted_md) - args.out.write(html) - - -if __name__ == "__main__": - sys.exit(main()) From 5858f3f4f62ab48ac8771806afaeb380b73ad537 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:30:29 +0000 Subject: [PATCH 085/344] Change default resources --- .../conf/base.config | 26 +++++++++++-------- 1 file changed, 15 insertions(+), 11 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config index 580f505c1e..578d73b0e5 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config @@ -13,7 +13,7 @@ process { // TODO nf-core: Check the defaults for all processes cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 7.GB * task.attempt, 'memory' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } @@ -21,31 +21,35 @@ process { maxErrors = '-1' // Process-specific resource requirements - // NOTE - Only one of the labels below are used in the fastqc process in the main script. + // NOTE - Please try and re-use the labels below as much as possible. + // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when - // adding in your processes. + // adding in your local modules too. // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 14.GB * task.attempt, 'memory' ) } - time = { check_max( 6.h * task.attempt, 'time' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } } withLabel:process_medium { cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 42.GB * task.attempt, 'memory' ) } + memory = { check_max( 36.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 84.GB * task.attempt, 'memory' ) } - time = { check_max( 10.h * task.attempt, 'time' ) } + memory = { check_max( 72 .GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } } withLabel:process_long { time = { check_max( 20.h * task.attempt, 'time' ) } } - withName:GET_SOFTWARE_VERSIONS { - cache = false + withLabel:error_ignore { + errorStrategy = 'ignore' } - + withLabel:error_retry { + errorStrategy = 'retry' + maxRetries = 2 + } } From b6b43e159f70c1491db0c3f1d0d84fc3ed8728fa Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:31:31 +0000 Subject: [PATCH 086/344] Replace baseDir with projectDir --- .../conf/igenomes.config | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config index 8fb4b874e9..eae4e6abde 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config @@ -21,7 +21,7 @@ params { readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" mito_name = "MT" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/GRCh37-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" @@ -33,7 +33,7 @@ params { bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" mito_name = "chrM" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" } 'GRCm38' { fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" @@ -46,7 +46,7 @@ params { readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" mito_name = "MT" macs_gsize = "1.87e9" - blacklist = "${baseDir}/assets/blacklists/GRCm38-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" } 'TAIR10' { fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" @@ -270,7 +270,7 @@ params { bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" mito_name = "chrM" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/hg38-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" } 'hg19' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" @@ -283,7 +283,7 @@ params { readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" mito_name = "chrM" macs_gsize = "2.7e9" - blacklist = "${baseDir}/assets/blacklists/hg19-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" } 'mm10' { fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" @@ -296,7 +296,7 @@ params { readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" mito_name = "chrM" macs_gsize = "1.87e9" - blacklist = "${baseDir}/assets/blacklists/mm10-blacklist.bed" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" } 'bosTau8' { fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" From bbe6b4ca12a9d4905f8b40e0a005fdcb8179f422 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:34:03 +0000 Subject: [PATCH 087/344] Initial commit --- .../conf/modules.config | 35 +++++++++++++++++++ 1 file changed, 35 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config new file mode 100644 index 0000000000..145a7253f9 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config @@ -0,0 +1,35 @@ +/* + * -------------------------------------------------- + * Config file for defining DSL2 per module options + * -------------------------------------------------- + * + * Available keys to override module options: + * args = Additional arguments appended to command in module. + * args2 = Second set of arguments appended to command in module (multi-tool modules). + * publish_dir = Directory to publish results. + * publish_by_id = Publish results in separate folders by meta.id value. + * publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + * The value of "directory" is appended to the standard "publish_dir" path as defined above. + * If publish_files == null (unspecified) - All files are published. + * If publish_files == false - No files are published. + * suffix = File name suffix for output files. + * + */ + +params { + modules { + 'cat_fastq' { + publish_dir = 'fastq' + } + 'fastqc' { + args = "--quiet" + } + 'trimgalore' { + args = "--fastqc" + publish_files = ['txt':'', 'html':'fastqc', 'zip':'fastqc'] + } + 'multiqc' { + args = "" + } + } +} From ea513494126212c9450b75fc517ec2c51c233106 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:35:07 +0000 Subject: [PATCH 088/344] Reduce default time for -profile test --- .../{{cookiecutter.name_noslash}}/conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config index a9a282735e..2770679004 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config @@ -13,7 +13,7 @@ params { // Limit resources so that this can run on GitHub Actions max_cpus = 2 max_memory = 6.GB - max_time = 48.h + max_time = 6.h // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets From 127ca4faf5a07d5e951aee7b93d7af0af2b7f8ca Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:43:18 +0000 Subject: [PATCH 089/344] Bloody Black again --- .../bin/scrape_software_versions.py | 26 ++++++++++--------- 1 file changed, 14 insertions(+), 12 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py index 3a767bab21..16aab7d971 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/scrape_software_versions.py @@ -3,19 +3,20 @@ import os results = {} -version_files = [x for x in os.listdir('.') if x.endswith('.version.txt')] +version_files = [x for x in os.listdir(".") if x.endswith(".version.txt")] for version_file in version_files: - software = version_file.replace('.version.txt','') - if software == 'pipeline': - software = '{{ cookiecutter.name }}' + software = version_file.replace(".version.txt", "") + if software == "pipeline": + software = "{{ cookiecutter.name }}" with open(version_file) as fin: version = fin.read().strip() results[software] = version # Dump to YAML -print (''' +print( + """ id: 'software_versions' section_name: '{{ cookiecutter.name }} Software Versions' section_href: 'https://github.com/{{ cookiecutter.name }}' @@ -23,12 +24,13 @@ description: 'are collected at run time from the software output.' data: |
-''') -for k,v in sorted(results.items()): - print("
{}
{}
".format(k,v)) -print ("
") +""" +) +for k, v in sorted(results.items()): + print("
{}
{}
".format(k, v)) +print(" ") # Write out regexes as csv file: -with open('software_versions.csv', 'w') as f: - for k,v in sorted(results.items()): - f.write("{}\t{}\n".format(k,v)) \ No newline at end of file +with open("software_versions.csv", "w") as f: + for k, v in sorted(results.items()): + f.write("{}\t{}\n".format(k, v)) From b3c3e34cbc6506c9387ccb820395d384f72afe12 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:49:37 +0000 Subject: [PATCH 090/344] Beautify --- .../conf/base.config | 26 +++++++++---------- 1 file changed, 13 insertions(+), 13 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config index 578d73b0e5..3e377aa613 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/base.config @@ -12,13 +12,13 @@ process { // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } + cpus = { check_max( 1 * task.attempt, 'cpus' ) } memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 1 - maxErrors = '-1' + maxRetries = 1 + maxErrors = '-1' // Process-specific resource requirements // NOTE - Please try and re-use the labels below as much as possible. @@ -28,28 +28,28 @@ process { // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } + cpus = { check_max( 2 * task.attempt, 'cpus' ) } memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } + cpus = { check_max( 6 * task.attempt, 'cpus' ) } memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72 .GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 72.GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } } withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { check_max( 20.h * task.attempt, 'time' ) } } withLabel:error_ignore { errorStrategy = 'ignore' } withLabel:error_retry { errorStrategy = 'retry' - maxRetries = 2 + maxRetries = 2 } } From d3174bbc801658be781e650cb10cc2d75e23544e Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:49:43 +0000 Subject: [PATCH 091/344] Beautify --- .../{{cookiecutter.name_noslash}}/conf/test.config | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config index 2770679004..01ba4e8f0b 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config @@ -8,12 +8,13 @@ */ params { - config_profile_name = 'Test profile' + config_profile_name = 'Test profile' config_profile_description = 'Minimal test dataset to check pipeline function' + // Limit resources so that this can run on GitHub Actions - max_cpus = 2 + max_cpus = 2 max_memory = 6.GB - max_time = 6.h + max_time = 6.h // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets From 4f20bd92be32a6206ba49956c34424ec589de6d1 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:49:47 +0000 Subject: [PATCH 092/344] Beautify --- .../{{cookiecutter.name_noslash}}/conf/test_full.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config index 5cc0a683e3..fce9932907 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config @@ -8,7 +8,7 @@ */ params { - config_profile_name = 'Full test profile' + config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test From 5bad649b69edb23704af3ee97c5997914171a481 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:50:09 +0000 Subject: [PATCH 093/344] Remove markdown_to_html dependencies --- .../{{cookiecutter.name_noslash}}/environment.yml | 8 +------- 1 file changed, 1 insertion(+), 7 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml index 04ed0dafb2..f5acdc9c5d 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml @@ -7,12 +7,6 @@ channels: - defaults dependencies: # TODO nf-core: Add required software dependencies here - ## conda-forge packages - - conda-forge::python=3.8.3 - - conda-forge::markdown=3.2.2 - - conda-forge::pymdown-extensions=7.1 - - conda-forge::pygments=2.6.1 - - ## bioconda packages - bioconda::fastqc=0.11.9 + - bioconda::trim-galore=0.6.5 - bioconda::multiqc=1.9 From 032869154d154dc341168dfd0fa7ef380367b2cb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:53:26 +0000 Subject: [PATCH 094/344] Update Headers.groovy --- .../lib/Headers.groovy | 30 +++++++++++-------- 1 file changed, 17 insertions(+), 13 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy index 8fd9c8c6fc..15d1d38800 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Headers.groovy @@ -6,34 +6,38 @@ class Headers { private static Map log_colours(Boolean monochrome_logs) { Map colorcodes = [:] - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" return colorcodes } + static String dashed_line(monochrome_logs) { + Map colors = log_colours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + static String nf_core(workflow, monochrome_logs) { Map colors = log_colours(monochrome_logs) String.format( """\n - -${colors.dim}----------------------------------------------------${colors.reset}- + ${dashed_line(monochrome_logs)} ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} ${colors.green}`._,._,\'${colors.reset} ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} - -${colors.dim}----------------------------------------------------${colors.reset}- + ${dashed_line(monochrome_logs)} """.stripIndent() ) } } - From 26f191e17b7ee58d4daacd241068e0ca762a8a75 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 17:55:51 +0000 Subject: [PATCH 095/344] Update Checks.groovy --- .../lib/Checks.groovy | 14 +++++++++++++- 1 file changed, 13 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy index 0c912c401b..f9103a5599 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy @@ -32,5 +32,17 @@ class Checks { } } } -} + // Citation string + private static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.1400710\n\n" + + "* The nf-core framework\n" + + " https://dx.doi.org/10.1038/s41587-020-0439-x\n" + + " https://rdcu.be/b1GjZ\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + +} \ No newline at end of file From 8321aeedc1ddbc2b41a91478e7ba936027508959 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 18:00:51 +0000 Subject: [PATCH 096/344] Update Completion.groovy --- .../lib/Completion.groovy | 98 ++++++++++--------- 1 file changed, 53 insertions(+), 45 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy index e709701333..e745c98653 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Completion.groovy @@ -3,7 +3,7 @@ */ class Completion { - static void email(workflow, params, summary, run_name, baseDir, multiqc_report, log) { + static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { // Set up the e-mail variables def subject = "[$workflow.manifest.name] Successful: $workflow.runName" @@ -11,37 +11,45 @@ class Completion { subject = "[$workflow.manifest.name] FAILED: $workflow.runName" } + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = run_name ?: workflow.runName - email_fields['success'] = workflow.success + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary - email_fields['summary']['Date Started'] = workflow.start - email_fields['summary']['Date Completed'] = workflow.complete - email_fields['summary']['Pipeline script file path'] = workflow.scriptFile - email_fields['summary']['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) email_fields['summary']['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) email_fields['summary']['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) email_fields['summary']['Pipeline Git branch/tag'] = workflow.revision - email_fields['summary']['Nextflow Version'] = workflow.nextflow.version - email_fields['summary']['Nextflow Build'] = workflow.nextflow.build - email_fields['summary']['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - // TODO nf-core: If not using MultiQC, strip out this code (including params.max_multiqc_email_size) + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + // On success try attach the multiqc report def mqc_report = null try { - if (workflow.success) { + if (workflow.success && !params.skip_multiqc) { mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } mqc_report = mqc_report[0] } } @@ -56,37 +64,39 @@ class Completion { } // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$baseDir/assets/email_template.txt") + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() + def email_txt = txt_template.toString() // Render the HTML template - def hf = new File("$baseDir/assets/email_template.html") + def hf = new File("$projectDir/assets/email_template.html") def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() + def email_html = html_template.toString() // Render the sendmail template - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, baseDir: "$baseDir", mqcFile: mqc_report, mqcMaxSize: params.max_multiqc_email_size.toBytes() ] - def sf = new File("$baseDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() // Send the HTML e-mail + Map colors = Headers.log_colours(params.monochrome_logs) if (email_address) { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "[$workflow.manifest.name] Sent summary e-mail to $email_address (sendmail)" + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= params.max_multiqc_email_size.toBytes() ) { + if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html - log.info "[$workflow.manifest.name] Sent summary e-mail to $email_address (mail)" + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" } } @@ -103,17 +113,15 @@ class Completion { static void summary(workflow, params, log) { Map colors = Headers.log_colours(params.monochrome_logs) - if (workflow.stats.ignoredCount > 0 && workflow.success) { - log.info "-${colors.purple}Warning, pipeline completed, but with errored process(es) ${colors.reset}-" - log.info "-${colors.red}Number of ignored errored process(es) : ${workflow.stats.ignoredCount} ${colors.reset}-" - log.info "-${colors.green}Number of successfully ran process(es) : ${workflow.stats.succeedCount} ${colors.reset}-" - } if (workflow.success) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } } else { Checks.hostname(workflow, params, log) log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" } } -} - +} \ No newline at end of file From 17f5ba1ff844b956305e6a84c6181df658eb0cf2 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 18:06:30 +0000 Subject: [PATCH 097/344] Update Schema.groovy --- .../lib/Schema.groovy | 232 ++++++++++++------ 1 file changed, 159 insertions(+), 73 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy index 5a7f6b51f1..e241f0c548 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy @@ -8,10 +8,10 @@ class Schema { /* * This method tries to read a JSON params file */ - private static LinkedHashMap params_get(String path) { + private static LinkedHashMap params_load(String json_schema) { def params_map = new LinkedHashMap() try { - params_map = params_try(path) + params_map = params_read(json_schema) } catch (Exception e) { println "Could not read parameters settings from JSON. $e" params_map = new LinkedHashMap() @@ -28,115 +28,201 @@ class Schema { Group - */ - private static LinkedHashMap params_try(String path) throws Exception { - - def json = new File(path).text - def Map json_params = (Map) new JsonSlurper().parseText(json).get('properties') - + private static LinkedHashMap params_read(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map json_params = (Map) new JsonSlurper().parseText(json).get('definitions') /* Tree looks like this in nf-core schema - * properties <- this is what the first get('properties') gets us + * definitions <- this is what the first get('definitions') gets us group 1 - properties + title description + properties + parameter 1 + type + description + parameter 2 + type + description group 2 - properties - description - group 3 - properties + title description + properties + parameter 1 + type + description */ def params_map = new LinkedHashMap() json_params.each { key, val -> def Map group = json_params."$key".properties // Gets the property object of the group + def title = json_params."$key".title def sub_params = new LinkedHashMap() group.each { innerkey, value -> - sub_params.put("$innerkey", [ "$value.type", "$value.description" ]) + sub_params.put(innerkey, value) } - params_map.put("$key", sub_params) + params_map.put(title, sub_params) } return params_map } + /* + * Get maximum number of characters across all parameter names + */ private static Integer params_max_chars(params_map) { Integer max_chars = 0 for (group in params_map.keySet()) { - def params = params_map.get(group) // This gets the parameters of that particular group - for (par in params.keySet()) { - if (par.size() > max_chars) { - max_chars = par.size() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() } } } return max_chars } - private static String params_beautify(params_map) { - String output = "" - def max_chars = params_max_chars(params_map) + 1 + /* + * Beautify parameters for --help + */ + private static String params_help(workflow, params, json_schema, command) { + String output = Headers.nf_core(workflow, params.monochrome_logs) + "\n" + output += "Typical pipeline command:\n\n" + output += " ${command}\n\n" + def params_map = params_load(json_schema) + def max_chars = params_max_chars(params_map) + 1 for (group in params_map.keySet()) { output += group + "\n" - def params = params_map.get(group) // This gets the parameters of that particular group - for (par in params.keySet()) { - def type = "[" + params.get(par)[0] + "]" - def description = params.get(par)[1] - output+= " \u001B[1m" + par.padRight(max_chars) + "\u001B[1m" + type.padRight(10) + description + "\n" + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + def type = "[" + group_params.get(param).type + "]" + def description = group_params.get(param).description + output += " \u001B[1m--" + param.padRight(max_chars) + "\u001B[1m" + type.padRight(10) + description + "\n" } output += "\n" } + output += Headers.dashed_line(params.monochrome_logs) + output += "\n\n" + Checks.citation(workflow) + output += "\n\n" + Headers.dashed_line(params.monochrome_logs) return output } - private static String params_help(path, command) { - String output = "Typical pipeline command:\n\n" - output += " ${command}\n\n" - output += params_beautify(params_get(path)) - } + /* + * Groovy Map summarising parameters/workflow options used by the pipeline + */ + private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + // Get a selection of core Nextflow workflow options + def Map workflow_summary = [:] + if (workflow.revision) { + workflow_summary['revision'] = workflow.revision + } + workflow_summary['runName'] = workflow.runName + if (workflow.containerEngine) { + workflow_summary['containerEngine'] = "$workflow.containerEngine" + } + if (workflow.container) { + workflow_summary['container'] = "$workflow.container" + } + workflow_summary['launchDir'] = workflow.launchDir + workflow_summary['workDir'] = workflow.workDir + workflow_summary['projectDir'] = workflow.projectDir + workflow_summary['userName'] = workflow.userName + workflow_summary['profile'] = workflow.profile + workflow_summary['configFiles'] = workflow.configFiles.join(', ') + + // Get pipeline parameters defined in JSON Schema + def Map params_summary = [:] + def blacklist = ['hostnames'] + def params_map = params_load(json_schema) + for (group in params_map.keySet()) { + def sub_params = new LinkedHashMap() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (params.containsKey(param) && !blacklist.contains(param)) { + def params_value = params.get(param) + def schema_value = group_params.get(param).default + def param_type = group_params.get(param).type + if (schema_value == null) { + if (param_type == 'boolean') { + schema_value = false + } + if (param_type == 'string') { + schema_value = '' + } + if (param_type == 'integer') { + schema_value = 0 + } + } else { + if (param_type == 'string') { + if (schema_value.contains('$baseDir') || schema_value.contains('${baseDir}')) { + def sub_string = schema_value.replace('\$baseDir','') + sub_string = sub_string.replace('\${baseDir}','') + if (params_value.contains(sub_string)) { + schema_value = params_value + } + } + if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { + def sub_string = schema_value.replace('\$params.outdir','') + sub_string = sub_string.replace('\${params.outdir}','') + if ("${params.outdir}${sub_string}" == params_value) { + schema_value = params_value + } + } + } + } - private static LinkedHashMap params_summary(workflow, params, run_name) { - def Map summary = [:] - if (workflow.revision) summary['Pipeline Release'] = workflow.revision - summary['Run Name'] = run_name ?: workflow.runName - // TODO nf-core: Report custom parameters here - summary['Input'] = params.input - summary['Fasta File'] = params.fasta - summary['Max Resources'] = "$params.max_memory memory, $params.max_cpus cpus, $params.max_time time per job" - if (workflow.containerEngine) summary['Container'] = "$workflow.containerEngine - $workflow.container" - summary['Output dir'] = params.outdir - summary['Launch dir'] = workflow.launchDir - summary['Working dir'] = workflow.workDir - summary['Script dir'] = workflow.projectDir - summary['User'] = workflow.userName - if (workflow.profile.contains('awsbatch')) { - summary['AWS Region'] = params.awsregion - summary['AWS Queue'] = params.awsqueue - summary['AWS CLI'] = params.awscli + if (params_value != schema_value) { + sub_params.put("$param", params_value) + } + } + } + params_summary.put(group, sub_params) } - summary['Config Profile'] = workflow.profile - if (params.config_profile_description) summary['Config Profile Descr'] = params.config_profile_description - if (params.config_profile_contact) summary['Config Profile Contact'] = params.config_profile_contact - if (params.config_profile_url) summary['Config Profile URL'] = params.config_profile_url - summary['Config Files'] = workflow.configFiles.join(', ') - if (params.email || params.email_on_fail) { - summary['E-mail Address'] = params.email - summary['E-mail on failure'] = params.email_on_fail - summary['MultiQC maxsize'] = params.max_multiqc_email_size + return [ 'Core Nextflow options' : workflow_summary ] << params_summary + } + + /* + * Beautify parameters for summary and return as string + */ + private static String params_summary_log(workflow, params, json_schema) { + String output = Headers.nf_core(workflow, params.monochrome_logs) + "\n" + def params_map = params_summary_map(workflow, params, json_schema) + def max_chars = params_max_chars(params_map) + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + if (group_params) { + output += group + "\n" + for (param in group_params.keySet()) { + output += " \u001B[1m" + param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + "\n" + } + output += "\n" + } } - return summary + output += Headers.dashed_line(params.monochrome_logs) + output += "\n\n" + Checks.citation(workflow) + output += "\n\n" + Headers.dashed_line(params.monochrome_logs) + return output } - static String params_mqc_summary(summary) { - String yaml_file_text = """ - id: '{{ cookiecutter.name_noslash }}-summary' - description: " - this information is collected when the pipeline is started." - section_name: '{{ cookiecutter.name }} Workflow Summary' - section_href: 'https://github.com/{{ cookiecutter.name }}' - plot_type: 'html' - data: | -
- ${summary.collect { k,v -> "
$k
${v ?: 'N/A'}
" }.join("\n")} -
- """.stripIndent() + static String params_summary_multiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "
\n" + } + } + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" return yaml_file_text } -} +} \ No newline at end of file From 5223944a018d5954e5df95931edd75e7535bfd32 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 18:19:02 +0000 Subject: [PATCH 098/344] Update nextflow.config --- .../nextflow.config | 96 +++++++++++-------- 1 file changed, 55 insertions(+), 41 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index b9c00bae5e..0feb105ec9 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -8,36 +8,45 @@ // Global default params, used in configs params { - // Workflow flags // TODO nf-core: Specify your pipeline's command line flags - genome = false - input = './samplesheet.csv' - outdir = './results' - publish_dir_mode = 'copy' + // Input options + input = '' + + // References + genome = '' + igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_ignore = false + + // MultiQC options + multiqc_config = '' + multiqc_title = '' + max_multiqc_email_size = '25.MB' // Boilerplate options - name = false - multiqc_config = false - email = false - email_on_fail = false - max_multiqc_email_size = 25.MB - plaintext_email = false - monochrome_logs = false - help = false - igenomes_base = 's3://ngi-igenomes/igenomes/' - tracedir = "${params.outdir}/pipeline_info" - igenomes_ignore = false - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = false - config_profile_description = false - config_profile_contact = false - config_profile_url = false + enable_conda = false + outdir = './results' + tracedir = "${params.outdir}/pipeline_info" + publish_dir_mode = 'copy' + email = '' + email_on_fail = '' + plaintext_email = false + monochrome_logs = false + help = false + clusterOptions = '' + + // Config options + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + hostnames = [:] + config_profile_description = '' + config_profile_contact = '' + config_profile_url = '' + // Max resource options // Defaults only, expecting to be overwritten - max_memory = 128.GB - max_cpus = 16 - max_time = 240.h + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' } @@ -48,6 +57,9 @@ process.container = '{{ cookiecutter.name_docker }}:dev' // Load base.config by default for all pipelines includeConfig 'conf/base.config' +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + // Load nf-core custom profiles from different Institutions try { includeConfig "${params.custom_config_base}/nfcore_custom.config" @@ -56,10 +68,10 @@ try { } profiles { - conda { process.conda = "$projectDir/environment.yml" } debug { process.beforeScript = 'echo $HOSTNAME' } + conda { params.enable_conda = true } docker { - docker.enabled = true + docker.enabled = true // Avoid this error: // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 @@ -67,26 +79,28 @@ profiles { docker.runOptions = '-u \$(id -u):\$(id -g)' } singularity { - singularity.enabled = true + singularity.enabled = true singularity.autoMounts = true } podman { podman.enabled = true } - test { includeConfig 'conf/test.config' } + test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } } // Load igenomes.config if required if (!params.igenomes_ignore) { includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] } // Export these variables to prevent local Python/R libraries from conflicting with those in the container env { PYTHONNOUSERSITE = 1 - R_PROFILE_USER = "/.Rprofile" - R_ENVIRON_USER = "/.Renviron" + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" } // Capture exit codes from upstream processes when piping @@ -94,29 +108,29 @@ process.shell = ['/bin/bash', '-euo', 'pipefail'] timeline { enabled = true - file = "${params.tracedir}/execution_timeline.html" + file = "${params.tracedir}/execution_timeline.html" } report { enabled = true - file = "${params.tracedir}/execution_report.html" + file = "${params.tracedir}/execution_report.html" } trace { enabled = true - file = "${params.tracedir}/execution_trace.txt" + file = "${params.tracedir}/execution_trace.txt" } dag { enabled = true - file = "${params.tracedir}/pipeline_dag.svg" + file = "${params.tracedir}/pipeline_dag.svg" } manifest { - name = '{{ cookiecutter.name }}' - author = '{{ cookiecutter.author }}' - homePage = 'https://github.com/{{ cookiecutter.name }}' - description = '{{ cookiecutter.description }}' - mainScript = 'main.nf' + name = '{{ cookiecutter.name }}' + author = '{{ cookiecutter.author }}' + homePage = 'https://github.com/{{ cookiecutter.name }}' + description = '{{ cookiecutter.description }}' + mainScript = 'main.nf' nextflowVersion = '>=20.04.0' - version = '{{ cookiecutter.version }}' + version = '{{ cookiecutter.version }}' } // Function to ensure that resource requirements don't go beyond From b3b1e9e6f4560c9ca51dc6f9c810a6112ea0f08d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 18:25:39 +0000 Subject: [PATCH 099/344] Add local modules --- .../modules/local/check_samplesheet.nf | 34 ----------- .../modules/local/get_software_versions.nf | 24 -------- .../modules/local/output_documentation.nf | 18 ------ .../modules/local/process/cat_fastq.nf | 54 +++++++++++++++++ .../modules/local/process/functions.nf | 59 +++++++++++++++++++ .../local/process/get_software_versions.nf | 32 ++++++++++ .../local/process/samplesheet_check.nf | 44 ++++++++++++++ 7 files changed, 189 insertions(+), 76 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/functions.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/get_software_versions.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf deleted file mode 100644 index 8eb356f762..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/check_samplesheet.nf +++ /dev/null @@ -1,34 +0,0 @@ -/* - * Reformat input samplesheet and check validity - */ -process CHECK_SAMPLESHEET { - tag "$samplesheet" - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode - - input: - path samplesheet - - output: - path '*.csv' - - script: // This script is bundled with the pipeline, in {{ cookiecutter.name }}/bin/ - """ - check_samplesheet.py $samplesheet samplesheet.valid.csv - """ -} - -// Function to get list of [ sample, single_end?, [ fastq_1, fastq_2 ] ] -def check_samplesheet_paths(LinkedHashMap samplesheet) { - def sample = samplesheet.sample - def single_end = samplesheet.single_end.toBoolean() - def fastq_1 = samplesheet.fastq_1 - def fastq_2 = samplesheet.fastq_2 - - def array = [] - if (single_end) { - array = [ sample, single_end, [ file(fastq_1, checkIfExists: true) ] ] - } else { - array = [ sample, single_end, [ file(fastq_1, checkIfExists: true), file(fastq_2, checkIfExists: true) ] ] - } - return array -} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf deleted file mode 100644 index b583603404..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/get_software_versions.nf +++ /dev/null @@ -1,24 +0,0 @@ -/* - * Parse software version numbers - */ -process GET_SOFTWARE_VERSIONS { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode, - saveAs: { filename -> - if (filename.indexOf(".csv") > 0) filename - else null - } - - output: - path 'software_versions_mqc.yaml', emit: yml - path "software_versions.csv", emit: csv - - script: - // TODO nf-core: Get all tools to print their version number here - """ - echo $workflow.manifest.version > v_pipeline.txt - echo $workflow.nextflow.version > v_nextflow.txt - fastqc --version > v_fastqc.txt - multiqc --version > v_multiqc.txt - scrape_software_versions.py &> software_versions_mqc.yaml - """ -} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf deleted file mode 100644 index b6d24b0f58..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/output_documentation.nf +++ /dev/null @@ -1,18 +0,0 @@ -/* - * Output Markdown documentation to HTML - */ -process OUTPUT_DOCUMENTATION { - publishDir "${params.outdir}/pipeline_info", mode: params.publish_dir_mode - - input: - path output_docs - path images - - output: - path "results_description.html" - - script: - """ - markdown_to_html.py $output_docs -o results_description.html - """ -} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf new file mode 100644 index 0000000000..4270303fad --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf @@ -0,0 +1,54 @@ +// Import generic module functions +include { initOptions; saveFiles } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +/* + * Concatenate FastQ files + */ +process CAT_FASTQ { + tag "$meta.id" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', publish_id:meta.id) } + + conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + container "biocontainers/biocontainers:v1.2.0_cv1" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.merged.fastq.gz"), emit: reads + + script: + def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" + readList = reads.collect{it.toString()} + if (!meta.single_end) { + if (readList.size > 2) { + def read1 = [] + def read2 = [] + readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } + """ + cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz + cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz + """ + } else { + """ + ln -s ${reads[0]} ${prefix}_1.merged.fastq.gz + ln -s ${reads[1]} ${prefix}_2.merged.fastq.gz + """ + } + } else { + if (readList.size > 1) { + """ + cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz + """ + } else { + """ + ln -s $reads ${prefix}.merged.fastq.gz + """ + } + } +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/functions.nf new file mode 100644 index 0000000000..b3ac38015b --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/get_software_versions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/get_software_versions.nf new file mode 100644 index 0000000000..7ad040579c --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/get_software_versions.nf @@ -0,0 +1,32 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +/* + * Parse software version numbers + */ +process GET_SOFTWARE_VERSIONS { + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + container "quay.io/biocontainers/python:3.8.3" + + cache false + + input: + path versions + + output: + path "software_versions.csv" , emit: csv + path 'software_versions_mqc.yaml', emit: yaml + + script: + """ + echo $workflow.manifest.version > pipeline.version.txt + echo $workflow.nextflow.version > nextflow.version.txt + scrape_software_versions.py &> software_versions_mqc.yaml + """ +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf new file mode 100644 index 0000000000..c883d8e930 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf @@ -0,0 +1,44 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +/* + * Reformat design file and check validity + */ +process SAMPLESHEET_CHECK { + tag "$samplesheet" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + container "quay.io/biocontainers/python:3.8.3" + + input: + path samplesheet + + output: + path '*.csv' + + + script: // This script is bundled with the pipeline, in {{ cookiecutter.name }}/bin/ + """ + check_samplesheet.py $samplesheet samplesheet.valid.csv + """ +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def get_samplesheet_paths(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + def array = [] + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1, checkIfExists: true) ] ] + } else { + array = [ meta, [ file(row.fastq_1, checkIfExists: true), file(row.fastq_2, checkIfExists: true) ] ] + } + return array +} From 9d6a20a5e8970562dd3a0278a174332f2c6bec2f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 18:29:48 +0000 Subject: [PATCH 100/344] Add local subworkflow --- .../modules/local/subworkflow/input_check.nf | 23 +++++++++++++++++++ 1 file changed, 23 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/subworkflow/input_check.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/subworkflow/input_check.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/subworkflow/input_check.nf new file mode 100644 index 0000000000..0d84cef955 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/subworkflow/input_check.nf @@ -0,0 +1,23 @@ +/* + * Check input samplesheet and get read channels + */ + +params.options = [:] + +include { + SAMPLESHEET_CHECK; + get_samplesheet_paths } from '../process/samplesheet_check' addParams( options: params.options ) + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .splitCsv ( header:true, sep:',' ) + .map { get_samplesheet_paths(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] +} From 398283217501a25eb1d8b850df9dc7014095f8d3 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 23 Nov 2020 18:32:16 +0000 Subject: [PATCH 101/344] Add FastQC module from nf-core/modules --- .../modules/nf-core/fastqc.nf | 32 --------- .../modules/nf-core/multiqc.nf | 36 ---------- .../nf-core/software/fastqc/functions.nf | 59 ++++++++++++++++ .../modules/nf-core/software/fastqc/main.nf | 43 ++++++++++++ .../modules/nf-core/software/fastqc/meta.yml | 67 +++++++++++++++++++ 5 files changed, 169 insertions(+), 68 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/functions.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf deleted file mode 100644 index 5129ab4884..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/fastqc.nf +++ /dev/null @@ -1,32 +0,0 @@ -/* - * FastQC - */ -process FASTQC { - tag "$name" - label 'process_medium' - publishDir "${params.outdir}/fastqc", mode: params.publish_dir_mode, - saveAs: { filename -> - filename.indexOf(".zip") > 0 ? "zips/$filename" : "$filename" - } - - input: - tuple val(name), val(single_end), path(reads) - - output: - path "*.{zip,html}" - - script: - // Add soft-links to original FastQs for consistent naming in pipeline - if (single_end) { - """ - [ ! -f ${name}.fastq.gz ] && ln -s $reads ${name}.fastq.gz - fastqc --quiet --threads $task.cpus ${name}.fastq.gz - """ - } else { - """ - [ ! -f ${name}_1.fastq.gz ] && ln -s ${reads[0]} ${name}_1.fastq.gz - [ ! -f ${name}_2.fastq.gz ] && ln -s ${reads[1]} ${name}_2.fastq.gz - fastqc --quiet --threads $task.cpus ${name}_1.fastq.gz ${name}_2.fastq.gz - """ - } -} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf deleted file mode 100644 index 6cec640723..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/multiqc.nf +++ /dev/null @@ -1,36 +0,0 @@ -// Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name -custom_runName = params.name -if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - custom_runName = workflow.runName -} - -/* - * MultiQC - */ -process MULTIQC { - publishDir "${params.outdir}/multiqc", mode: params.publish_dir_mode - - input: - path multiqc_config - path mqc_custom_config - // TODO nf-core: Add in log files from your new processes for MultiQC to find! - path fastqc - path software_versions - val workflow_summary - - output: - path "*multiqc_report.html" - path "*_data" - path "multiqc_plots" - - script: - rtitle = custom_runName ? "--title \"$custom_runName\"" : '' - rfilename = custom_runName ? "--filename " + custom_runName.replaceAll('\\W','_').replaceAll('_+','_') + "_multiqc_report" : '' - custom_config_file = params.multiqc_config ? "--config $mqc_custom_config" : '' - // TODO nf-core: Specify which MultiQC modules to use with -m for a faster run time - """ - echo '$workflow_summary' > workflow_summary_mqc.yaml - multiqc -f $rtitle $rfilename $custom_config_file . - """ -} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/functions.nf new file mode 100644 index 0000000000..b3ac38015b --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf new file mode 100644 index 0000000000..3edc97a14e --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf @@ -0,0 +1,43 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process FASTQC { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + + conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) + container "quay.io/biocontainers/fastqc:0.11.9--0" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "*.version.txt" , emit: version + + script: + // Add soft-links to original FastQs for consistent naming in pipeline + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz + fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt + """ + } else { + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz + fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt + """ + } +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml new file mode 100644 index 0000000000..337fc372b4 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml @@ -0,0 +1,67 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" From eb165e13865c376092212e289c66a252d82fa02d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 11:50:26 +0000 Subject: [PATCH 102/344] Fix AWS batch error --- .../{{cookiecutter.name_noslash}}/lib/Checks.groovy | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy index f9103a5599..0167d07f7f 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy @@ -6,12 +6,12 @@ class Checks { static void aws_batch(workflow, params) { if (workflow.profile.contains('awsbatch')) { - assert !params.awsqueue || !params.awsregion : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" // Check outdir paths to be S3 buckets if running on AWSBatch // related: https://github.com/nextflow-io/nextflow/issues/813 - assert !params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" // Prevent trace files to be stored on S3 since S3 does not support rolling files. - assert params.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + assert !params.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." } } From d376b0064d5b641def063179246c8e21243821ea Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 12:13:04 +0000 Subject: [PATCH 103/344] Update Schema --- .../nextflow_schema.json | 206 +++++++++--------- 1 file changed, 103 insertions(+), 103 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json index e6bab501f0..2b482a7f7b 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json @@ -17,12 +17,12 @@ "input": { "type": "string", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a samplesheet with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/{{ cookiecutter.short_name }}/docs/usage#--input).", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location.", "fa_icon": "fas fa-file-csv" }, "outdir": { "type": "string", - "description": "The output directory where the results will be saved.", + "description": "Path to the output directory where the results will be saved.", "default": "./results", "fa_icon": "fas fa-folder-open" }, @@ -39,19 +39,19 @@ "title": "Reference genome options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for the reference genome indices used to align reads.", + "description": "Reference genome related files and options required for the workflow.", "properties": { "genome": { "type": "string", "description": "Name of iGenomes reference.", "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." }, "fasta": { "type": "string", - "fa_icon": "fas fa-font", "description": "Path to FASTA genome file.", - "help_text": "If you have no genome reference available, the pipeline can build one using a FASTA file. This requires additional time and resources, so it's better to use a pre-build index if possible." + "help_text": "This parameter is *mandatory* if `--genome` is not specified.", + "fa_icon": "far fa-file-code" }, "igenomes_base": { "type": "string", @@ -69,84 +69,51 @@ } } }, - "generic_options": { - "title": "Generic options", + "institutional_config_options": { + "title": "Institutional config options", "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "hidden": true, - "fa_icon": "fas fa-question-circle" - }, - "publish_dir_mode": { + "custom_config_version": { "type": "string", - "default": "copy", + "description": "Git commit id for Institutional configs.", + "default": "master", "hidden": true, - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ] + "fa_icon": "fas fa-users-cog" }, - "name": { + "custom_config_base": { "type": "string", - "description": "Workflow name.", - "fa_icon": "fas fa-fingerprint", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", "hidden": true, - "help_text": "A custom name for the pipeline run. Unlike the core nextflow `-name` option with one hyphen this parameter can be reused multiple times, for example if using `-resume`. Passed through to steps such as MultiQC and used for things like report filenames and titles." + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" }, - "email_on_fail": { + "hostnames": { "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "hidden": true, - "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", + "description": "Institutional configs hostname.", "hidden": true, - "help_text": "Set to receive plain-text e-mails instead of HTML formatted." + "fa_icon": "fas fa-users-cog" }, - "max_multiqc_email_size": { + "config_profile_description": { "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true, - "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", + "description": "Institutional config description.", "hidden": true, - "help_text": "Set to disable colourful command line output and live life in monochrome." + "fa_icon": "fas fa-users-cog" }, - "multiqc_config": { + "config_profile_contact": { "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" }, - "tracedir": { + "config_profile_url": { "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" } } }, @@ -159,7 +126,7 @@ "properties": { "max_cpus": { "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", + "description": "Maximum number of CPUs that can be requested for any single job.", "default": 16, "fa_icon": "fas fa-microchip", "hidden": true, @@ -183,52 +150,85 @@ } } }, - "institutional_config_options": { - "title": "Institutional config options", + "generic_options": { + "title": "Generic options", "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", "properties": { - "custom_config_version": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog", - "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```" + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true }, - "custom_config_base": { + "email_on_fail": { "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.", - "fa_icon": "fas fa-users-cog" + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true }, - "hostnames": { + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "tracedir": { "type": "string", - "description": "Institutional configs hostname.", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", "hidden": true, - "fa_icon": "fas fa-users-cog" + "fa_icon": "fas fa-bacon" }, - "config_profile_description": { + "multiqc_config": { "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true }, - "config_profile_contact": { + "multiqc_title": { "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" }, - "config_profile_url": { + "max_multiqc_email_size": { "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "File size limit when attaching MultiQC reports to summary emails.", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true } } } @@ -241,13 +241,13 @@ "$ref": "#/definitions/reference_genome_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/definitions/institutional_config_options" }, { "$ref": "#/definitions/max_job_request_options" }, { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/definitions/generic_options" } ] -} +} \ No newline at end of file From 7982708c99b5ba3afd7651bf6ca9ade7e86fafc6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 12:13:13 +0000 Subject: [PATCH 104/344] Update config --- .../{{cookiecutter.name_noslash}}/nextflow.config | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index 0feb105ec9..e3d0fcd5a1 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -32,7 +32,6 @@ params { plaintext_email = false monochrome_logs = false help = false - clusterOptions = '' // Config options custom_config_version = 'master' From 91013eb4136c07c0926388427079cfe741a9199a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 12:44:28 +0000 Subject: [PATCH 105/344] Add big warning to top of file --- .../{{cookiecutter.name_noslash}}/Dockerfile | 16 ++++++++++++++++ 1 file changed, 16 insertions(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile index bd07987b6e..47753bdce7 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile @@ -1,3 +1,19 @@ +################################################################################### +##################################### WARNING ##################################### +################################################################################### +## If using DSL2 then you no longer need this file unless you need to build and ## +## host a custom container for your pipeline that contains software dependencies ## +## that are unavailable on Conda. ## +## ## +## All Conda dependencies can be installed by a combination of using: ## +## - The Nextflow 'conda' directive to list conda package requirements ## +## - The Nextflow 'container' directive to list Docker / Singularity images ## +## hosted by Biocontainers ## +## ## +## See an example implementation of FastQC on nf-core/modules: ## +## https://github.com/nf-core/modules/blob/master/software/fastqc/main.nf ## +################################################################################### + FROM nfcore/base:{{ 'dev' if 'dev' in cookiecutter.nf_core_version else cookiecutter.nf_core_version }} LABEL authors="{{ cookiecutter.author }}" \ description="Docker image containing all software requirements for the {{ cookiecutter.name }} pipeline" From 233d649ab69b108eb378bf4e4d7fec3133b639c0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 12:44:39 +0000 Subject: [PATCH 106/344] Add big warning to top of file --- .../environment.yml | 17 +++++++++++++++-- 1 file changed, 15 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml index f5acdc9c5d..c9da642c28 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml @@ -1,3 +1,17 @@ +################################################################################### +##################################### WARNING ##################################### +################################################################################### +## If using DSL2 then you no longer need this file. ## +## ## +## All Conda dependencies can be installed by a combination of using: ## +## - The Nextflow 'conda' directive to list conda package requirements ## +## - The Nextflow 'container' directive to list Docker / Singularity images ## +## hosted by Biocontainers ## +## ## +## See an example implementation of FastQC on nf-core/modules: ## +## https://github.com/nf-core/modules/blob/master/software/fastqc/main.nf ## +################################################################################### + # You can use this file to create a conda environment for this pipeline: # conda env create -f environment.yml name: {{ cookiecutter.name_noslash }}-{{ cookiecutter.version }} @@ -8,5 +22,4 @@ channels: dependencies: # TODO nf-core: Add required software dependencies here - bioconda::fastqc=0.11.9 - - bioconda::trim-galore=0.6.5 - - bioconda::multiqc=1.9 + - bioconda::multiqc=1.9 \ No newline at end of file From 420247537190f97f5311b9cdf239b3ae628febce Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 12:47:04 +0000 Subject: [PATCH 107/344] Remove cat fastq process --- .../modules/local/process/cat_fastq.nf | 54 ------------------- 1 file changed, 54 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf deleted file mode 100644 index 4270303fad..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/cat_fastq.nf +++ /dev/null @@ -1,54 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles } from './functions' - -params.options = [:] -def options = initOptions(params.options) - -/* - * Concatenate FastQ files - */ -process CAT_FASTQ { - tag "$meta.id" - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'merged_fastq', publish_id:meta.id) } - - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) - container "biocontainers/biocontainers:v1.2.0_cv1" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.merged.fastq.gz"), emit: reads - - script: - def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" - readList = reads.collect{it.toString()} - if (!meta.single_end) { - if (readList.size > 2) { - def read1 = [] - def read2 = [] - readList.eachWithIndex{ v, ix -> ( ix & 1 ? read2 : read1 ) << v } - """ - cat ${read1.sort().join(' ')} > ${prefix}_1.merged.fastq.gz - cat ${read2.sort().join(' ')} > ${prefix}_2.merged.fastq.gz - """ - } else { - """ - ln -s ${reads[0]} ${prefix}_1.merged.fastq.gz - ln -s ${reads[1]} ${prefix}_2.merged.fastq.gz - """ - } - } else { - if (readList.size > 1) { - """ - cat ${readList.sort().join(' ')} > ${prefix}.merged.fastq.gz - """ - } else { - """ - ln -s $reads ${prefix}.merged.fastq.gz - """ - } - } -} From 9fb35bcc6ce75e18028fa58b7ad15b86c0ac5da5 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 12:48:37 +0000 Subject: [PATCH 108/344] Use braces for version --- .../{{cookiecutter.name_noslash}}/CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CHANGELOG.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CHANGELOG.md index b401036075..8fcebe19a2 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CHANGELOG.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v{{ cookiecutter.version }} - [date] +## [{{ cookiecutter.version }}] - [date] Initial release of {{ cookiecutter.name }}, created with the [nf-core](https://nf-co.re/) template. From 7466405b5ff1459faef66e52fb98d8bc29ff16ba Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 13:26:14 +0000 Subject: [PATCH 109/344] Initial commit --- .../modules/local/process/multiqc.nf | 34 +++++++++++++++++++ 1 file changed, 34 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf new file mode 100644 index 0000000000..b20df745ed --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf @@ -0,0 +1,34 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process MULTIQC { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } + + conda (params.enable_conda ? "bioconda::multiqc=1.9" : null) + container "quay.io/biocontainers/multiqc:1.9--pyh9f0ad1d_0" + + input: + path multiqc_config + path multiqc_custom_config + path software_versions + path workflow_summary + path multiqc_files + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + + script: + def software = getSoftwareName(task.process) + def custom_config = params.multiqc_config ? "--config $multiqc_custom_config" : '' + """ + multiqc -f $options.args $custom_config . + """ +} From 3863c51d82ece2b54fd3244554ff6fa20cd8893f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 13:30:37 +0000 Subject: [PATCH 110/344] Add new badges --- .../{{cookiecutter.name_noslash}}/README.md | 20 ++++++++++++------- 1 file changed, 13 insertions(+), 7 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index e0a5376177..44f7ef4b0f 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -1,17 +1,23 @@ # ![{{ cookiecutter.name }}](docs/images/{{ cookiecutter.name_noslash }}_logo.png) -**{{ cookiecutter.description }}**. +[![GitHub Actions CI Status](https://github.com/{{ cookiecutter.name }}/workflows/nf-core%20CI/badge.svg)](https://github.com/{{ cookiecutter.name }}/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/{{ cookiecutter.name }}/workflows/nf-core%20linting/badge.svg)](https://github.com/{{ cookiecutter.name }}/actions?query=workflow%3A%22nf-core+linting%22) +[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/{{ cookiecutter.short_name }}/results) +[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![GitHub Actions CI Status](https://github.com/{{ cookiecutter.name }}/workflows/nf-core%20CI/badge.svg)](https://github.com/{{ cookiecutter.name }}/actions) -[![GitHub Actions Linting Status](https://github.com/{{ cookiecutter.name }}/workflows/nf-core%20linting/badge.svg)](https://github.com/{{ cookiecutter.name }}/actions) -[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A520.04.0-brightgreen.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A520.07.1-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) +[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) +[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) +[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/) -[![Docker](https://img.shields.io/docker/automated/{{ cookiecutter.name_docker }}.svg)](https://hub.docker.com/r/{{ cookiecutter.name_docker }}) -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23{{ cookiecutter.short_name }}-4A154B?logo=slack)](https://nfcore.slack.com/channels/{{ cookiecutter.short_name }}) +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23{{ cookiecutter.short_name }}-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/{{ cookiecutter.short_name }}) +[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core) +[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) ## Introduction +**{{ cookiecutter.description }}**. + The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. ## Quick Start From 60c10fc17c1cc5ba22c44b5ba20539f91d023a98 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 13:36:09 +0000 Subject: [PATCH 111/344] Update README --- .../{{cookiecutter.name_noslash}}/README.md | 15 ++++++++++++--- 1 file changed, 12 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index 44f7ef4b0f..a1989f8b1f 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -20,6 +20,16 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. + +On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/{{ cookiecutter.short_name }}/results). + +## Pipeline summary + + + +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) + ## Quick Start 1. Install [`nextflow`](https://nf-co.re/usage/installation) @@ -33,6 +43,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool ``` > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > * If you are using `singularity`, it is highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. 4. Start running your own analysis! @@ -48,8 +59,6 @@ See [usage docs](https://nf-co.re/{{ cookiecutter.short_name }}/usage) for all o The {{ cookiecutter.name }} pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/{{ cookiecutter.short_name }}/usage) and [output](https://nf-co.re/{{ cookiecutter.short_name }}/output). - - ## Credits {{ cookiecutter.name }} was originally written by {{ cookiecutter.author }}. @@ -62,7 +71,7 @@ For further information or help, don't hesitate to get in touch on the [Slack `# ## Citation - + You can cite the `nf-core` publication as follows: From 8aca25812e6c154b32421b6c3a20aee8c98e368e Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 13:48:02 +0000 Subject: [PATCH 112/344] Update Nextflow version --- .../{{cookiecutter.name_noslash}}/.github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml index 1e27cafe67..d188c667c7 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml @@ -20,7 +20,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.04.0', ''] + nxf_ver: ['20.07.1', ''] steps: - name: Check out pipeline code uses: actions/checkout@v2 From ceff91457b5f591d50107ec91e53a7ae1167f0b6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 13:48:06 +0000 Subject: [PATCH 113/344] Update Nextflow version --- .../{{cookiecutter.name_noslash}}/nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index e3d0fcd5a1..214909ca19 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -128,7 +128,7 @@ manifest { homePage = 'https://github.com/{{ cookiecutter.name }}' description = '{{ cookiecutter.description }}' mainScript = 'main.nf' - nextflowVersion = '>=20.04.0' + nextflowVersion = '>=20.07.1' version = '{{ cookiecutter.version }}' } From e15e07f02de19b9871f3de81d81a18ab830b71ee Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 14:23:58 +0000 Subject: [PATCH 114/344] First pass implementation based on rnaseq --- .../{{cookiecutter.name_noslash}}/main.nf | 202 +++++++++++------- 1 file changed, 122 insertions(+), 80 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 90facb93fb..729b4d89de 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -9,111 +9,153 @@ ---------------------------------------------------------------------------------------- */ -nextflow.preview.dsl = 2 +nextflow.enable.dsl = 2 -/* - * Print help message if required - */ +//////////////////////////////////////////////////// +/* -- PRINT HELP -- */ +//////////////////////////////////////////////////// + +def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { // TODO nf-core: Update typical command used to run pipeline def command = "nextflow run {{ cookiecutter.name }} --input samplesheet.csv -profile docker" - log.info Headers.nf_core(workflow, params.monochrome_logs) - log.info Schema.params_help("$baseDir/nextflow_schema.json", command) + log.info Schema.params_help(workflow, params, json_schema, command) exit 0 } -/* - * Stage config files - */ -ch_multiqc_config = file("$baseDir/assets/multiqc_config.yaml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() -ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true) -ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true) +//////////////////////////////////////////////////// +/* -- DEFAULT PARAMETER VALUES -- */ +//////////////////////////////////////////////////// -/* - * Validate parameters - */ -if (params.input) { ch_input = file(params.input, checkIfExists: true) } else { exit 1, "Input samplesheet file not specified!" } - -/* - * Reference genomes - */ -// TODO nf-core: Add any reference files that are needed -// NOTE - FOR SIMPLICITY THIS IS NOT USED IN THIS PIPELINE -// EXAMPLE ONLY TO DEMONSTRATE USAGE OF AWS IGENOMES +// Check if genome exists in the config file if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "The provided genome '${params.genome}' is not available in the iGenomes file. Currently the available genomes are ${params.genomes.keySet().join(", ")}" + exit 1, "Genome '${params.genome}' was not found in any config files made available to the pipeline. Currently, the available genomes are ${params.genomes.keySet().join(", ")}" } -params.fasta = params.genomes[params.genome]?.fasta -if (params.fasta) { ch_fasta = file(params.fasta, checkIfExists: true) } -/* - * Check parameters - */ -Checks.aws_batch(workflow, params) // Check AWS batch settings -Checks.hostname(workflow, params, log) // Check the hostnames against configured profiles +//////////////////////////////////////////////////// +/* -- VALIDATE INPUTS -- */ +//////////////////////////////////////////////////// -/* - * Print parameter summary - */ -// Has the run name been specified by the user? -// this has the bonus effect of catching both -name and --name -run_name = params.name -if (!(workflow.runName ==~ /[a-z]+_[a-z]+/)) { - run_name = workflow.runName -} -summary = Schema.params_summary(workflow, params, run_name) -log.info Headers.nf_core(workflow, params.monochrome_logs) -log.info summary.collect { k,v -> "${k.padRight(20)}: $v" }.join("\n") -log.info "-\033[2m----------------------------------------------------\033[0m-" +// TODO nf-core: Add all file path parameters for the pipeline to the list below +// Check input path parameters to see if they exist +checkPathParamList = [ + params.input +] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } -workflow_summary = Schema.params_mqc_summary(summary) -ch_workflow_summary = Channel.value(workflow_summary) +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } -/* - * Include local pipeline modules - */ -include { OUTPUT_DOCUMENTATION } from './modules/local/output_documentation' params(params) -include { GET_SOFTWARE_VERSIONS } from './modules/local/get_software_versions' params(params) -include { CHECK_SAMPLESHEET; check_samplesheet_paths } from './modules/local/check_samplesheet' params(params) +//////////////////////////////////////////////////// +/* -- CONFIG FILES -- */ +//////////////////////////////////////////////////// -/* - * Include nf-core modules - */ -include { FASTQC } from './modules/nf-core/fastqc' params(params) -include { MULTIQC } from './modules/nf-core/multiqc' params(params) +ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() -/* - * Run the workflow - */ -workflow { +//////////////////////////////////////////////////// +/* -- PRINT PARAMETER SUMMARY -- */ +//////////////////////////////////////////////////// - CHECK_SAMPLESHEET(ch_input) - .splitCsv(header:true, sep:',') - .map { check_samplesheet_paths(it) } - .set { ch_raw_reads } +def summary_params = Schema.params_summary_map(workflow, params, json_schema) +log.info Schema.params_summary_log(workflow, params, json_schema) - FASTQC(ch_raw_reads) +//////////////////////////////////////////////////// +/* -- PARAMETER CHECKS -- */ +//////////////////////////////////////////////////// - OUTPUT_DOCUMENTATION( - ch_output_docs, - ch_output_docs_images) +// Check AWS batch settings +Checks.aws_batch(workflow, params) - GET_SOFTWARE_VERSIONS() +// Check the hostnames against configured profiles +Checks.hostname(workflow, params, log) - MULTIQC( +//////////////////////////////////////////////////// +/* -- IMPORT MODULES / SUBWORKFLOWS -- */ +//////////////////////////////////////////////////// + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. +def modules = params.modules.clone() + +def multiqc_options = modules['multiqc'] +multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' + +// Local: Modules +include { MULTIQC } from './modules/local/process/multiqc' addParams( options: multiqc_options ) +include { GET_SOFTWARE_VERSIONS } from './modules/local/process/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) + +// Local: Sub-workflows +include { INPUT_CHECK } from './modules/local/subworkflow/input_check' addParams( options: [:] ) + +// nf-core/modules: Modules +include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) + +//////////////////////////////////////////////////// +/* -- RUN MAIN WORKFLOW -- */ +//////////////////////////////////////////////////// + +// Info required for completion email and summary +def multiqc_report = [] + +workflow { + + ch_software_versions = Channel.empty() + + /* + * SUBWORKFLOW: Read in samplesheet, validate and stage input files + */ + INPUT_CHECK ( + ch_input + ) + // .map { + // meta, fastq -> + // meta.id = meta.id.split('_')[0..-2].join('_') + // [ meta, bam ] } + // .groupTuple(by: [0]) + // .map { it -> [ it[0], it[1].flatten() ] } + // .set { ch_reads } + + /* + * MODULE: Run FastQC + */ + FASTQC ( + INPUT_CHECK.out.reads + ) + ch_software_versions = ch_software_versions.mix(FASTQC_UMITOOLS_TRIMGALORE.out.fastqc_version.first().ifEmpty(null)) + + + /* + * MODULE: Pipeline reporting + */ + GET_SOFTWARE_VERSIONS ( + ch_software_versions.map { it }.collect() + ) + + /* + * MultiQC + */ + workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + MULTIQC ( ch_multiqc_config, ch_multiqc_custom_config.collect().ifEmpty([]), - FASTQC.out.collect(), - GET_SOFTWARE_VERSIONS.out.yml.collect(), - ch_workflow_summary) + GET_SOFTWARE_VERSIONS.out.yaml.collect(), + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'), + FASTQC.out.zip.collect{it[1]}.ifEmpty([]), + ) + multiqc_report = MULTIQC.out.report.toList() } -/* - * Send completion email - */ +//////////////////////////////////////////////////// +/* -- COMPLETION EMAIL -- */ +//////////////////////////////////////////////////// + workflow.onComplete { - def multiqc_report = [] - Completion.email(workflow, params, summary, run_name, baseDir, multiqc_report, log) + Completion.email(workflow, params, summary_params, projectDir, log, multiqc_report) Completion.summary(workflow, params, log) } + +//////////////////////////////////////////////////// +/* -- THE END -- */ +//////////////////////////////////////////////////// \ No newline at end of file From 2a5066942ac984a7b8ec22c0a9820d287b9f2339 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 14:29:24 +0000 Subject: [PATCH 115/344] Refine main script --- .../{{cookiecutter.name_noslash}}/main.nf | 13 +++---------- 1 file changed, 3 insertions(+), 10 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 729b4d89de..ff8a508a5f 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -107,13 +107,6 @@ workflow { INPUT_CHECK ( ch_input ) - // .map { - // meta, fastq -> - // meta.id = meta.id.split('_')[0..-2].join('_') - // [ meta, bam ] } - // .groupTuple(by: [0]) - // .map { it -> [ it[0], it[1].flatten() ] } - // .set { ch_reads } /* * MODULE: Run FastQC @@ -121,7 +114,7 @@ workflow { FASTQC ( INPUT_CHECK.out.reads ) - ch_software_versions = ch_software_versions.mix(FASTQC_UMITOOLS_TRIMGALORE.out.fastqc_version.first().ifEmpty(null)) + ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) /* @@ -134,8 +127,8 @@ workflow { /* * MultiQC */ - workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) + workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) MULTIQC ( ch_multiqc_config, From 0dbc222cf5689d521a7db772b89f353d34893628 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 14:31:19 +0000 Subject: [PATCH 116/344] Remove environment.yml --- .../environment.yml | 25 ------------------- 1 file changed, 25 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml deleted file mode 100644 index c9da642c28..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/environment.yml +++ /dev/null @@ -1,25 +0,0 @@ -################################################################################### -##################################### WARNING ##################################### -################################################################################### -## If using DSL2 then you no longer need this file. ## -## ## -## All Conda dependencies can be installed by a combination of using: ## -## - The Nextflow 'conda' directive to list conda package requirements ## -## - The Nextflow 'container' directive to list Docker / Singularity images ## -## hosted by Biocontainers ## -## ## -## See an example implementation of FastQC on nf-core/modules: ## -## https://github.com/nf-core/modules/blob/master/software/fastqc/main.nf ## -################################################################################### - -# You can use this file to create a conda environment for this pipeline: -# conda env create -f environment.yml -name: {{ cookiecutter.name_noslash }}-{{ cookiecutter.version }} -channels: - - conda-forge - - bioconda - - defaults -dependencies: - # TODO nf-core: Add required software dependencies here - - bioconda::fastqc=0.11.9 - - bioconda::multiqc=1.9 \ No newline at end of file From 697aecb8038a650b091c89aa6be78333a25843b2 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 14:31:37 +0000 Subject: [PATCH 117/344] Remove Dockerfile --- .../{{cookiecutter.name_noslash}}/Dockerfile | 33 ------------------- 1 file changed, 33 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile deleted file mode 100644 index 47753bdce7..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/Dockerfile +++ /dev/null @@ -1,33 +0,0 @@ -################################################################################### -##################################### WARNING ##################################### -################################################################################### -## If using DSL2 then you no longer need this file unless you need to build and ## -## host a custom container for your pipeline that contains software dependencies ## -## that are unavailable on Conda. ## -## ## -## All Conda dependencies can be installed by a combination of using: ## -## - The Nextflow 'conda' directive to list conda package requirements ## -## - The Nextflow 'container' directive to list Docker / Singularity images ## -## hosted by Biocontainers ## -## ## -## See an example implementation of FastQC on nf-core/modules: ## -## https://github.com/nf-core/modules/blob/master/software/fastqc/main.nf ## -################################################################################### - -FROM nfcore/base:{{ 'dev' if 'dev' in cookiecutter.nf_core_version else cookiecutter.nf_core_version }} -LABEL authors="{{ cookiecutter.author }}" \ - description="Docker image containing all software requirements for the {{ cookiecutter.name }} pipeline" - -# Install the conda environment -COPY environment.yml / -RUN conda env create --quiet -f /environment.yml && conda clean -a - -# Add conda installation dir to PATH (instead of doing 'conda activate') -ENV PATH /opt/conda/envs/{{ cookiecutter.name_noslash }}-{{ cookiecutter.version }}/bin:$PATH - -# Dump the details of the installed packages to a file for posterity -RUN conda env export --name {{ cookiecutter.name_noslash }}-{{ cookiecutter.version }} > {{ cookiecutter.name_noslash }}-{{ cookiecutter.version }}.yml - -# Instruct R processes to use these empty files instead of clashing with a local version -RUN touch .Rprofile -RUN touch .Renviron From 6b3a051d6ebc9d1ef9c92116aab30eae46f762f2 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 14:57:18 +0000 Subject: [PATCH 118/344] Remove process.container --- .../{{cookiecutter.name_noslash}}/nextflow.config | 4 ---- 1 file changed, 4 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index 214909ca19..b8315353bb 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -49,10 +49,6 @@ params { } -// Container slug. Stable releases should specify release tag! -// Developmental code should specify :dev -process.container = '{{ cookiecutter.name_docker }}:dev' - // Load base.config by default for all pipelines includeConfig 'conf/base.config' From 4ca1dd9a573a47d902af30c33fd2002dd4310930 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 14:59:44 +0000 Subject: [PATCH 119/344] Fix markdownlint --- .../pipeline-template/{{cookiecutter.name_noslash}}/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index a1989f8b1f..3b98be13e7 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -42,7 +42,7 @@ On release, automated continuous integration tests run the pipeline on a full-si nextflow run {{ cookiecutter.name }} -profile test, ``` - > Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. > * If you are using `singularity`, it is highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. 4. Start running your own analysis! From 59cebf6a1833d3301c20045ace64e3f39cf49cb0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:04:59 +0000 Subject: [PATCH 120/344] Remove container related actions --- .../.github/workflows/ci.yml | 17 ----------------- 1 file changed, 17 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml index d188c667c7..0145a76d32 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml @@ -25,23 +25,6 @@ jobs: - name: Check out pipeline code uses: actions/checkout@v2 - - name: Check if Dockerfile or Conda environment changed - uses: technote-space/get-diff-action@v4 - with: - FILES: | - Dockerfile - environment.yml - - - name: Build new docker image - if: env.MATCHED_FILES - run: docker build --no-cache . -t {{ cookiecutter.name_docker }}:dev - - - name: Pull docker image - if: {% raw %}${{ !env.MATCHED_FILES }}{% endraw %} - run: | - docker pull {{ cookiecutter.name_docker }}:dev - docker tag {{ cookiecutter.name_docker }}:dev {{ cookiecutter.name_docker }}:dev - - name: Install Nextflow env: CAPSULE_LOG: none From 3fe60ae1e3fc206eddae46d4b713cde4716dae0c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:05:30 +0000 Subject: [PATCH 121/344] Delete Github actions to push Docker containers --- .../.github/workflows/push_dockerhub_dev.yml | 28 ------------------ .../workflows/push_dockerhub_release.yml | 29 ------------------- 2 files changed, 57 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_dev.yml delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_release.yml diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_dev.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_dev.yml deleted file mode 100644 index 0303241f10..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_dev.yml +++ /dev/null @@ -1,28 +0,0 @@ -name: nf-core Docker push (dev) -# This builds the docker image and pushes it to DockerHub -# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) -on: - push: - branches: - - dev - -jobs: - push_dockerhub: - name: Push new Docker image to Docker Hub (dev) - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: {% raw %}${{{% endraw %} github.repository == '{{ cookiecutter.name }}' {% raw %}}}{% endraw %} - env: - DOCKERHUB_USERNAME: {% raw %}${{ secrets.DOCKERHUB_USERNAME }}{% endraw %} - DOCKERHUB_PASS: {% raw %}${{ secrets.DOCKERHUB_PASS }}{% endraw %} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 - - - name: Build new docker image - run: docker build --no-cache . -t {{ cookiecutter.name_docker }}:dev - - - name: Push Docker image to DockerHub (dev) - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push {{ cookiecutter.name_docker }}:dev diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_release.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_release.yml deleted file mode 100644 index d5e260af96..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/push_dockerhub_release.yml +++ /dev/null @@ -1,29 +0,0 @@ -name: nf-core Docker push (release) -# This builds the docker image and pushes it to DockerHub -# Runs on nf-core repo releases and push event to 'dev' branch (PR merges) -on: - release: - types: [published] - -jobs: - push_dockerhub: - name: Push new Docker image to Docker Hub (release) - runs-on: ubuntu-latest - # Only run for the nf-core repo, for releases and merged PRs - if: {% raw %}${{{% endraw %} github.repository == '{{ cookiecutter.name }}' {% raw %}}}{% endraw %} - env: - DOCKERHUB_USERNAME: {% raw %}${{ secrets.DOCKERHUB_USERNAME }}{% endraw %} - DOCKERHUB_PASS: {% raw %}${{ secrets.DOCKERHUB_PASS }}{% endraw %} - steps: - - name: Check out pipeline code - uses: actions/checkout@v2 - - - name: Build new docker image - run: docker build --no-cache . -t {{ cookiecutter.name_docker }}:latest - - - name: Push Docker image to DockerHub (release) - run: | - echo "$DOCKERHUB_PASS" | docker login -u "$DOCKERHUB_USERNAME" --password-stdin - docker push {{ cookiecutter.name_docker }}:latest - docker tag {{ cookiecutter.name_docker }}:latest {{ cookiecutter.name_docker }}:{% raw %}${{ github.event.release.tag_name }}{% endraw %} - docker push {{ cookiecutter.name_docker }}:{% raw %}${{ github.event.release.tag_name }}{% endraw %} From 84c134e0e11d9013a4457645844bf64045c69281 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:13:06 +0000 Subject: [PATCH 122/344] Remove index paths --- .../conf/igenomes.config | 148 +++++++++--------- 1 file changed, 74 insertions(+), 74 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config index eae4e6abde..7485867ba5 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/igenomes.config @@ -12,8 +12,8 @@ params { genomes { 'GRCh37' { fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" @@ -25,8 +25,8 @@ params { } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" @@ -37,8 +37,8 @@ params { } 'GRCm38' { fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" @@ -50,8 +50,8 @@ params { } 'TAIR10' { fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" @@ -61,8 +61,8 @@ params { } 'EB2' { fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" @@ -71,8 +71,8 @@ params { } 'UMD3.1' { fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" @@ -82,8 +82,8 @@ params { } 'WBcel235' { fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" @@ -93,8 +93,8 @@ params { } 'CanFam3.1' { fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" @@ -104,8 +104,8 @@ params { } 'GRCz10' { fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" @@ -114,8 +114,8 @@ params { } 'BDGP6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" @@ -125,8 +125,8 @@ params { } 'EquCab2' { fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" @@ -136,8 +136,8 @@ params { } 'EB1' { fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" @@ -146,8 +146,8 @@ params { } 'Galgal4' { fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" @@ -156,8 +156,8 @@ params { } 'Gm01' { fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" @@ -166,8 +166,8 @@ params { } 'Mmul_1' { fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" @@ -177,8 +177,8 @@ params { } 'IRGSP-1.0' { fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" @@ -187,8 +187,8 @@ params { } 'CHIMP2.1.4' { fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" @@ -198,8 +198,8 @@ params { } 'Rnor_6.0' { fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" @@ -208,8 +208,8 @@ params { } 'R64-1-1' { fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" @@ -219,8 +219,8 @@ params { } 'EF2' { fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" @@ -231,8 +231,8 @@ params { } 'Sbi1' { fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" @@ -241,8 +241,8 @@ params { } 'Sscrofa10.2' { fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" @@ -252,8 +252,8 @@ params { } 'AGPv3' { fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" @@ -262,8 +262,8 @@ params { } 'hg38' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" @@ -274,8 +274,8 @@ params { } 'hg19' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" @@ -287,8 +287,8 @@ params { } 'mm10' { fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" @@ -300,8 +300,8 @@ params { } 'bosTau8' { fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" @@ -310,8 +310,8 @@ params { } 'ce10' { fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" @@ -322,8 +322,8 @@ params { } 'canFam3' { fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" @@ -333,8 +333,8 @@ params { } 'danRer10' { fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" @@ -343,8 +343,8 @@ params { } 'dm6' { fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" @@ -354,8 +354,8 @@ params { } 'equCab2' { fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" @@ -365,8 +365,8 @@ params { } 'galGal4' { fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" @@ -376,8 +376,8 @@ params { } 'panTro4' { fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" @@ -387,8 +387,8 @@ params { } 'rn6' { fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" @@ -397,8 +397,8 @@ params { } 'sacCer3' { fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" @@ -407,8 +407,8 @@ params { } 'susScr3' { fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa.{amb,ann,bwt,pac,sa}" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/genome.{1.bt2,2.bt2,3.bt2,4.bt2,rev.1.bt2,rev.2.bt2}" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" From f4aa25874f7cb01bfa01a22591d119ce1d50aeba Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:26:08 +0000 Subject: [PATCH 123/344] Use atacseq minimal samplesheet as example --- .../{{cookiecutter.name_noslash}}/conf/test.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config index 01ba4e8f0b..f8996ffc9e 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test.config @@ -19,8 +19,8 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_amplicon.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design.csv' // Genome references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.fna.gz' + genome = 'R64-1-1' } From 7c8302887c45c4007ef98a5a72860880a5ad1799 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:26:32 +0000 Subject: [PATCH 124/344] Use atacseq full samplesheet as example --- .../{{cookiecutter.name_noslash}}/conf/test_full.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config index fce9932907..649b2c9eb4 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/test_full.config @@ -14,8 +14,8 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_amplicon.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design_full.csv' // Genome references - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.fna.gz' + genome = 'GRCh37' } From faaa18403546a499b2513516a45a2b6aad6b2b40 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:26:42 +0000 Subject: [PATCH 125/344] Remove deleted module settings --- .../{{cookiecutter.name_noslash}}/conf/modules.config | 11 ++--------- 1 file changed, 2 insertions(+), 9 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config index 145a7253f9..f26191e0f2 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/conf/modules.config @@ -18,18 +18,11 @@ params { modules { - 'cat_fastq' { - publish_dir = 'fastq' - } 'fastqc' { - args = "--quiet" - } - 'trimgalore' { - args = "--fastqc" - publish_files = ['txt':'', 'html':'fastqc', 'zip':'fastqc'] + args = "--quiet" } 'multiqc' { - args = "" + args = "" } } } From 7d7e4ea54795005d93025fee7a53e7ee6e50513d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:41:13 +0000 Subject: [PATCH 126/344] Update example link --- .../{{cookiecutter.name_noslash}}/bin/check_samplesheet.py | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py index aa25cde165..ff0c52b1f5 100755 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/bin/check_samplesheet.py @@ -1,7 +1,8 @@ #!/usr/bin/env python # TODO nf-core: Update the script to check the samplesheet -# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_sispa.csv +# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design.csv + import os import sys From f201a6433866ee0742081fa7532e9928d7230faa Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:53:01 +0000 Subject: [PATCH 127/344] Add FastQC images for output docs --- .../docs/images/mqc_fastqc_adapter.png | Bin 0 -> 23458 bytes .../docs/images/mqc_fastqc_counts.png | Bin 0 -> 33918 bytes .../docs/images/mqc_fastqc_quality.png | Bin 0 -> 55769 bytes 3 files changed, 0 insertions(+), 0 deletions(-) create mode 100755 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/images/mqc_fastqc_adapter.png create mode 100755 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/images/mqc_fastqc_counts.png create mode 100755 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/images/mqc_fastqc_quality.png diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/images/mqc_fastqc_adapter.png b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/images/mqc_fastqc_adapter.png new file mode 100755 index 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zXwhtT%ei{n#FzEH|C;yZ>+$!u_x#*+`=L8{b9SH^9&27u3G_Gxqxe`L2UJtdxghk z&-wzDFvLvW{chK5u3{n6GSKKy!P&C6w^IFpbD0bcp^A{{2lcLh_DXj@ybtYvc^;(2 M)78&qol`;+0Fu7JivR!s literal 0 HcmV?d00001 From 049bdc3d873f7cd90ca9dbe5adfe219e6562a6f9 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:53:16 +0000 Subject: [PATCH 128/344] Allow HTML elements --- .../{{cookiecutter.name_noslash}}/.github/markdownlint.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/markdownlint.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/markdownlint.yml index 96b12a7039..dde95399ef 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/markdownlint.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/markdownlint.yml @@ -3,3 +3,5 @@ default: true, line-length: false no-duplicate-header: siblings_only: true +MD033: + allowed_elements: [details, summary] \ No newline at end of file From 6aaa65c1ce0d6f36d8dbda28dc00d405948270d3 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 15:53:25 +0000 Subject: [PATCH 129/344] Re-write output docs --- .../docs/output.md | 55 +++++++++++-------- 1 file changed, 33 insertions(+), 22 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/output.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/output.md index 966fefb2a7..f23a17e1d3 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/output.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/docs/output.md @@ -14,50 +14,61 @@ The directories listed below will be created in the results directory after the ## Pipeline overview -The pipeline is built using [Nextflow](https://www.nextflow.io/) -and processes data using the following steps: +The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -* [FastQC](#fastqc) - Read quality control -* [MultiQC](#multiqc) - Aggregate report describing results from the whole pipeline +* [FastQC](#fastqc) - Raw read QC +* [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline * [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -## FastQC +### FastQC -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. - -For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -**Output files:** +
+Output files * `fastqc/` * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files. -* `fastqc/zips/` * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +> **NB:** The FastQC plots in this directory are generated relative to the raw, input reads. They may contain adapter sequence and regions of low quality. To see how your reads look after adapter and quality trimming please refer to the FastQC reports in the `trimgalore/fastqc/` directory. + +
-## MultiQC +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). -[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarizing all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. +![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) -The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. +![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) + +![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) + +> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -For more information about how to use MultiQC reports, see [https://multiqc.info](https://multiqc.info). +### MultiQC -**Output files:** +
+Output files -* `multiqc/` +* `multiqc/` * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. * `multiqc_plots/`: directory containing static images from the report in various formats. -## Pipeline information +
-[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. +[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. + +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + +### Pipeline information -**Output files:** +
+Output files * `pipeline_info/` * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`. - * Documentation for interpretation of results in HTML format: `results_description.html`. + * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + +
+ +[Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. From 6aea9ba898ad2ac44e2a81d835e3929ef5decbc1 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 30 Nov 2020 17:54:51 +0000 Subject: [PATCH 130/344] Update errors for markdownlint --- .github/markdownlint.yml | 3 +++ 1 file changed, 3 insertions(+) diff --git a/.github/markdownlint.yml b/.github/markdownlint.yml index a0eac5a096..d67e660150 100644 --- a/.github/markdownlint.yml +++ b/.github/markdownlint.yml @@ -7,3 +7,6 @@ no-duplicate-header: no-bare-urls: false # tools only - suppresses error messages for usage of $ in main README commands-show-output: false +# Enable usage of nice HTML tags in output docs for pipeline template +MD033: + allowed_elements: [details, summary] From 22941dde1970363f6fd775e977035578336b59ba Mon Sep 17 00:00:00 2001 From: drpatelh Date: Thu, 3 Dec 2020 14:10:19 +0000 Subject: [PATCH 131/344] Add note about using Conda --- .../pipeline-template/{{cookiecutter.name_noslash}}/README.md | 1 + 1 file changed, 1 insertion(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index 3b98be13e7..51b54d7533 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -44,6 +44,7 @@ On release, automated continuous integration tests run the pipeline on a full-si > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. > * If you are using `singularity`, it is highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. + > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. 4. Start running your own analysis! From f6b509e6fd99950846f65402113c42b1479c6300 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Thu, 3 Dec 2020 17:52:06 +0000 Subject: [PATCH 132/344] Dont use checkIfExists here --- .../modules/local/process/samplesheet_check.nf | 12 +++++++++--- 1 file changed, 9 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf index c883d8e930..c4cdd1bdf3 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/samplesheet_check.nf @@ -35,10 +35,16 @@ def get_samplesheet_paths(LinkedHashMap row) { meta.single_end = row.single_end.toBoolean() def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } if (meta.single_end) { - array = [ meta, [ file(row.fastq_1, checkIfExists: true) ] ] + array = [ meta, [ file(row.fastq_1) ] ] } else { - array = [ meta, [ file(row.fastq_1, checkIfExists: true), file(row.fastq_2, checkIfExists: true) ] ] + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] } - return array + return array } From 16c26f4835887e140eb6b5b31824d94020ba461c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 7 Dec 2020 16:03:35 +0000 Subject: [PATCH 133/344] Add functions to check genome --- .../lib/Checks.groovy | 26 +++++++++++++++++-- 1 file changed, 24 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy index 0167d07f7f..3ef8aa0c74 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy @@ -44,5 +44,27 @@ class Checks { "* Software dependencies\n" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } - -} \ No newline at end of file + + // Exit pipeline if incorrect --genome key provided + static void genome_exists(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "=============================================================================" + System.exit(0) + } + } + + // Get attribute from genome config file e.g. fasta + static String get_genome_attribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} From b7698dadd1d860b6a993651cbf62945ce86f7e73 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 7 Dec 2020 16:03:48 +0000 Subject: [PATCH 134/344] Reorder sections and initialise genome params --- .../{{cookiecutter.name_noslash}}/main.nf | 51 ++++++++++--------- 1 file changed, 26 insertions(+), 25 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index ff8a508a5f..dce4956f33 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -24,13 +24,31 @@ if (params.help) { } //////////////////////////////////////////////////// -/* -- DEFAULT PARAMETER VALUES -- */ +/* -- PARAMETER CHECKS -- */ //////////////////////////////////////////////////// -// Check if genome exists in the config file -if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - exit 1, "Genome '${params.genome}' was not found in any config files made available to the pipeline. Currently, the available genomes are ${params.genomes.keySet().join(", ")}" -} +// Check AWS batch settings +Checks.aws_batch(workflow, params) + +// Check the hostnames against configured profiles +Checks.hostname(workflow, params, log) + +// Check genome key exists if provided +Checks.genome_exists(params, log) + +//////////////////////////////////////////////////// +/* -- GENOME PARAMETER VALUES -- */ +//////////////////////////////////////////////////// + +params.fasta = Checks.get_genome_attribute(params, 'fasta') +params.gtf = Checks.get_genome_attribute(params, 'gtf') + +//////////////////////////////////////////////////// +/* -- PRINT PARAMETER SUMMARY -- */ +//////////////////////////////////////////////////// + +def summary_params = Schema.params_summary_map(workflow, params, json_schema) +log.info Schema.params_summary_log(workflow, params, json_schema) //////////////////////////////////////////////////// /* -- VALIDATE INPUTS -- */ @@ -53,23 +71,6 @@ if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input sample ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() -//////////////////////////////////////////////////// -/* -- PRINT PARAMETER SUMMARY -- */ -//////////////////////////////////////////////////// - -def summary_params = Schema.params_summary_map(workflow, params, json_schema) -log.info Schema.params_summary_log(workflow, params, json_schema) - -//////////////////////////////////////////////////// -/* -- PARAMETER CHECKS -- */ -//////////////////////////////////////////////////// - -// Check AWS batch settings -Checks.aws_batch(workflow, params) - -// Check the hostnames against configured profiles -Checks.hostname(workflow, params, log) - //////////////////////////////////////////////////// /* -- IMPORT MODULES / SUBWORKFLOWS -- */ //////////////////////////////////////////////////// @@ -95,7 +96,7 @@ include { FASTQC } from './modules/nf-core/software/fastqc/main' //////////////////////////////////////////////////// // Info required for completion email and summary -def multiqc_report = [] +def multiqc_report = [] workflow { @@ -117,7 +118,7 @@ workflow { ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) - /* + /* * MODULE: Pipeline reporting */ GET_SOFTWARE_VERSIONS ( @@ -151,4 +152,4 @@ workflow.onComplete { //////////////////////////////////////////////////// /* -- THE END -- */ -//////////////////////////////////////////////////// \ No newline at end of file +//////////////////////////////////////////////////// From daa29cfd7abf0908dfd6d56e8adbb6a2cf228d0a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 7 Dec 2020 16:27:29 +0000 Subject: [PATCH 135/344] Move igenomes.config code up a bit --- .../{{cookiecutter.name_noslash}}/nextflow.config | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index b8315353bb..d80ed25280 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -62,6 +62,13 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } +// Load igenomes.config if required +if (!params.igenomes_ignore) { + includeConfig 'conf/igenomes.config' +} else { + params.genomes = [:] +} + profiles { debug { process.beforeScript = 'echo $HOSTNAME' } conda { params.enable_conda = true } @@ -84,13 +91,6 @@ profiles { test_full { includeConfig 'conf/test_full.config' } } -// Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} else { - params.genomes = [:] -} - // Export these variables to prevent local Python/R libraries from conflicting with those in the container env { PYTHONNOUSERSITE = 1 From e854cb34f42beae986c17be6df39df38b32fe90e Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:02:01 +0000 Subject: [PATCH 136/344] Update FastQC module --- .../modules/nf-core/software/fastqc/main.nf | 8 ++++++-- .../modules/nf-core/software/fastqc/meta.yml | 5 +++++ 2 files changed, 11 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf index 3edc97a14e..3cb510ac7e 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/main.nf @@ -11,8 +11,12 @@ process FASTQC { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } - conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) - container "quay.io/biocontainers/fastqc:0.11.9--0" + conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0" + } else { + container "quay.io/biocontainers/fastqc:0.11.9--0" + } input: tuple val(meta), path(reads) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml index 337fc372b4..413aad8db6 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/fastqc/meta.yml @@ -31,6 +31,11 @@ params: description: | Run the module with Conda using the software specified via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. input: - meta: type: map From e7e33626a65a36b00b70f933b23daba4e8578071 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:02:21 +0000 Subject: [PATCH 137/344] Add MultiQC module --- .../nf-core/software/multiqc/functions.nf | 59 ++++++++++++++++++ .../modules/nf-core/software/multiqc/main.nf | 35 +++++++++++ .../modules/nf-core/software/multiqc/meta.yml | 60 +++++++++++++++++++ 3 files changed, 154 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/functions.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/main.nf create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/meta.yml diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/functions.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/functions.nf new file mode 100644 index 0000000000..d25eea86b3 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/main.nf new file mode 100644 index 0000000000..ff1175fcd0 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process MULTIQC { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename: filename, options: params.options, publish_dir: getSoftwareName(task.process), publish_id: '') } + + conda (params.enable_conda ? "bioconda::multiqc=1.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/multiqc:1.9--pyh9f0ad1d_0" + } else { + container "quay.io/biocontainers/multiqc:1.9--pyh9f0ad1d_0" + } + + input: + path multiqc_files + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + multiqc -f $options.args . + multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt + """ +} diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/meta.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/meta.yml new file mode 100644 index 0000000000..02f4b41586 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/nf-core/software/multiqc/meta.yml @@ -0,0 +1,60 @@ +name: MultiQC +description: Aggregate results from bioinformatics analyses across many samples into a single report +keywords: + - QC + - bioinformatics tools + - Beautiful stand-alone HTML report +tools: + - multiqc: + description: | + MultiQC searches a given directory for analysis logs and compiles a HTML report. + It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. + homepage: https://multiqc.info/ + documentation: https://multiqc.info/docs/ +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC +output: + - report: + type: file + description: MultiQC report file + pattern: "multiqc_report.html" + - data: + type: dir + description: MultiQC data dir + pattern: "multiqc_data" + - plots: + type: file + description: Plots created by MultiQC + pattern: "*_data" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@abhi18av" + - "@bunop" + - "@drpatelh" From bd96bc2686453cb11562943362688ac0fad5c012 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:06:54 +0000 Subject: [PATCH 138/344] Use MultiQC from nf-core/modules --- .../{{cookiecutter.name_noslash}}/main.nf | 14 ++++++++------ 1 file changed, 8 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index dce4956f33..c02aa16452 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -82,7 +82,6 @@ def multiqc_options = modules['multiqc'] multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' // Local: Modules -include { MULTIQC } from './modules/local/process/multiqc' addParams( options: multiqc_options ) include { GET_SOFTWARE_VERSIONS } from './modules/local/process/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) // Local: Sub-workflows @@ -90,6 +89,7 @@ include { INPUT_CHECK } from './modules/local/subworkflow/input_check' // nf-core/modules: Modules include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from './modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) //////////////////////////////////////////////////// /* -- RUN MAIN WORKFLOW -- */ @@ -131,12 +131,14 @@ workflow { workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) + ch_multiqc_files = ch_multiqc_config + ch_multiqc_config.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_config.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_config.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_config.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + MULTIQC ( - ch_multiqc_config, - ch_multiqc_custom_config.collect().ifEmpty([]), - GET_SOFTWARE_VERSIONS.out.yaml.collect(), - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'), - FASTQC.out.zip.collect{it[1]}.ifEmpty([]), + ch_multiqc_files ) multiqc_report = MULTIQC.out.report.toList() } From 93794ee1d3eef60d167b55275f70ebc5b497b364 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:07:15 +0000 Subject: [PATCH 139/344] Remove local MultiQC module --- .../modules/local/process/multiqc.nf | 34 ------------------- 1 file changed, 34 deletions(-) delete mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf deleted file mode 100644 index b20df745ed..0000000000 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/modules/local/process/multiqc.nf +++ /dev/null @@ -1,34 +0,0 @@ -// Import generic module functions -include { initOptions; saveFiles; getSoftwareName } from './functions' - -params.options = [:] -def options = initOptions(params.options) - -process MULTIQC { - label 'process_medium' - publishDir "${params.outdir}", - mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:'') } - - conda (params.enable_conda ? "bioconda::multiqc=1.9" : null) - container "quay.io/biocontainers/multiqc:1.9--pyh9f0ad1d_0" - - input: - path multiqc_config - path multiqc_custom_config - path software_versions - path workflow_summary - path multiqc_files - - output: - path "*multiqc_report.html", emit: report - path "*_data" , emit: data - path "*_plots" , optional:true, emit: plots - - script: - def software = getSoftwareName(task.process) - def custom_config = params.multiqc_config ? "--config $multiqc_custom_config" : '' - """ - multiqc -f $options.args $custom_config . - """ -} From 98d3ca34f3cd24b4d7e87cfc8417ac3f332d3b0f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:11:21 +0000 Subject: [PATCH 140/344] Add --singularity_pull_docker_container to schema --- .../nextflow_schema.json | 19 +++++++++++++------ 1 file changed, 13 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json index 2b482a7f7b..2cbc696e66 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json @@ -206,12 +206,6 @@ "fa_icon": "fas fa-palette", "hidden": true }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" - }, "multiqc_config": { "type": "string", "description": "Custom config file to supply to MultiQC.", @@ -229,6 +223,19 @@ "default": "25.MB", "fa_icon": "fas fa-file-upload", "hidden": true + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", + "hidden": true, + "fa_icon": "fas fa-toolbox", + "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." } } } From 274ca7f9d218690aafaa013ea54ca22ce00d3efe Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:14:49 +0000 Subject: [PATCH 141/344] Bump NF version to 20.11.0-edge --- .../{{cookiecutter.name_noslash}}/nextflow.config | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index d80ed25280..06f0584863 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -23,7 +23,6 @@ params { max_multiqc_email_size = '25.MB' // Boilerplate options - enable_conda = false outdir = './results' tracedir = "${params.outdir}/pipeline_info" publish_dir_mode = 'copy' @@ -32,6 +31,8 @@ params { plaintext_email = false monochrome_logs = false help = false + enable_conda = false + singularity_pull_docker_container = false // Config options custom_config_version = 'master' @@ -124,7 +125,7 @@ manifest { homePage = 'https://github.com/{{ cookiecutter.name }}' description = '{{ cookiecutter.description }}' mainScript = 'main.nf' - nextflowVersion = '>=20.07.1' + nextflowVersion = '!>=20.11.0-edge' version = '{{ cookiecutter.version }}' } From 6aca9ce928fa8a0a094db83d3f4c4896f6893fce Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:21:05 +0000 Subject: [PATCH 142/344] Update README --- .../{{cookiecutter.name_noslash}}/README.md | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index 51b54d7533..e698d88e2b 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -5,7 +5,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/{{ cookiecutter.short_name }}/results) [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A520.07.1-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A520.11.0--edge-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -43,9 +43,10 @@ On release, automated continuous integration tests run the pipeline on a full-si ``` > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. - > * If you are using `singularity`, it is highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. + > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. It is also highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. + 4. Start running your own analysis! @@ -75,6 +76,8 @@ For further information or help, don't hesitate to get in touch on the [Slack `# +An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. + You can cite the `nf-core` publication as follows: > **The nf-core framework for community-curated bioinformatics pipelines.** @@ -82,4 +85,3 @@ You can cite the `nf-core` publication as follows: > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). -> ReadCube: [Full Access Link](https://rdcu.be/b1GjZ) From 7f5cedf6c87341a56817be4a45534f416530ca85 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:21:13 +0000 Subject: [PATCH 143/344] Add CITATIONS file --- .../CITATIONS.md | 32 +++++++++++++++++++ 1 file changed, 32 insertions(+) create mode 100644 nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CITATIONS.md diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CITATIONS.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CITATIONS.md new file mode 100644 index 0000000000..6435c19211 --- /dev/null +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/CITATIONS.md @@ -0,0 +1,32 @@ +# {{ cookiecutter.name }}: Citations + +## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) + +> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. + +## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) + +> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. + +## Pipeline tools + +* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + +* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + +## Software packaging/containerisation tools + +* [Anaconda](https://anaconda.com) + > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. + +* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) + > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. + +* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) + > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. + +* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + +* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. From bbb57fc409a974452525160e955c514e07d4c6cb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:26:35 +0000 Subject: [PATCH 144/344] Add conda channel check --- .../lib/Checks.groovy | 31 +++++++++++++++++++ 1 file changed, 31 insertions(+) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy index 3ef8aa0c74..16747ed845 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy @@ -2,8 +2,39 @@ * This file holds several functions used to perform standard checks for the nf-core pipeline template. */ +import org.yaml.snakeyaml.Yaml + class Checks { + static void check_conda_channels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + def required_channels = ['conda-forge', 'bioconda', 'defaults'] + def conda_check_failed = !required_channels.every { ch -> ch in channels } + + // Check that they are in the right order + conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) + conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + + if (conda_check_failed) { + log.warn "=============================================================================\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + + " NB: The order of the channels matters!\n" + + "===================================================================================" + } + } + static void aws_batch(workflow, params) { if (workflow.profile.contains('awsbatch')) { assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" From 50cbbd4dfa8efebcc75154c86656e9dd98c48e45 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 11:28:16 +0000 Subject: [PATCH 145/344] Replacec baseDir with projectDir --- .../{{cookiecutter.name_noslash}}/lib/Schema.groovy | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy index e241f0c548..c2cad355a5 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Schema.groovy @@ -152,9 +152,9 @@ class Schema { } } else { if (param_type == 'string') { - if (schema_value.contains('$baseDir') || schema_value.contains('${baseDir}')) { - def sub_string = schema_value.replace('\$baseDir','') - sub_string = sub_string.replace('\${baseDir}','') + if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { + def sub_string = schema_value.replace('\$projectDir','') + sub_string = sub_string.replace('\${projectDir}','') if (params_value.contains(sub_string)) { schema_value = params_value } @@ -225,4 +225,4 @@ class Schema { yaml_file_text += "${summary_section}" return yaml_file_text } -} \ No newline at end of file +} From 082e587f211210ab206dfce7686b398b915b56ba Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 12:23:56 +0000 Subject: [PATCH 146/344] Update NF version in ci.yml --- .../{{cookiecutter.name_noslash}}/.github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml index 0145a76d32..d5dba4b9b5 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/.github/workflows/ci.yml @@ -20,7 +20,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: ['20.07.1', ''] + nxf_ver: [20.11.0-edge] steps: - name: Check out pipeline code uses: actions/checkout@v2 From af65f1ca93a0a0d1adfc80c20a03871c8d28b8f4 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 12:26:08 +0000 Subject: [PATCH 147/344] Fix markdownlint --- .../pipeline-template/{{cookiecutter.name_noslash}}/README.md | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index e698d88e2b..d2e2d3a620 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -46,7 +46,6 @@ On release, automated continuous integration tests run the pipeline on a full-si > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. It is also highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. - 4. Start running your own analysis! From 36080e3fce20b1697263f2b315d8298d855c9549 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 12:37:08 +0000 Subject: [PATCH 148/344] Update main script --- .../{{cookiecutter.name_noslash}}/main.nf | 43 +++++++++++-------- 1 file changed, 24 insertions(+), 19 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index c02aa16452..9a9e193beb 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -23,25 +23,11 @@ if (params.help) { exit 0 } -//////////////////////////////////////////////////// -/* -- PARAMETER CHECKS -- */ -//////////////////////////////////////////////////// - -// Check AWS batch settings -Checks.aws_batch(workflow, params) - -// Check the hostnames against configured profiles -Checks.hostname(workflow, params, log) - -// Check genome key exists if provided -Checks.genome_exists(params, log) - //////////////////////////////////////////////////// /* -- GENOME PARAMETER VALUES -- */ //////////////////////////////////////////////////// params.fasta = Checks.get_genome_attribute(params, 'fasta') -params.gtf = Checks.get_genome_attribute(params, 'gtf') //////////////////////////////////////////////////// /* -- PRINT PARAMETER SUMMARY -- */ @@ -50,19 +36,36 @@ params.gtf = Checks.get_genome_attribute(params, 'gtf') def summary_params = Schema.params_summary_map(workflow, params, json_schema) log.info Schema.params_summary_log(workflow, params, json_schema) +//////////////////////////////////////////////////// +/* -- PARAMETER CHECKS -- */ +//////////////////////////////////////////////////// + +// Check that conda channels are set-up correctly +if (params.enable_conda) { + Checks.check_conda_channels(log) +} + +// Check AWS batch settings +Checks.aws_batch(workflow, params) + +// Check the hostnames against configured profiles +Checks.hostname(workflow, params, log) + +// Check genome key exists if provided +Checks.genome_exists(params, log) + //////////////////////////////////////////////////// /* -- VALIDATE INPUTS -- */ //////////////////////////////////////////////////// // TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist -checkPathParamList = [ - params.input -] +checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } +if (params.fasta) { ch_fasta = file(params.fasta) } else { exit 1, 'Genome fasta file not specified!' } //////////////////////////////////////////////////// /* -- CONFIG FILES -- */ @@ -131,7 +134,7 @@ workflow { workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) - ch_multiqc_files = ch_multiqc_config + ch_multiqc_files = ch_multiqc_config ch_multiqc_config.mix(ch_multiqc_custom_config.collect().ifEmpty([])) ch_multiqc_config.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_config.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) @@ -140,7 +143,9 @@ workflow { MULTIQC ( ch_multiqc_files ) - multiqc_report = MULTIQC.out.report.toList() + multiqc_report = MULTIQC.out.report.toList() + ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) + } //////////////////////////////////////////////////// From cf560b1f8212b03da407bf55bc26c07df4881f4f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 13:13:41 +0000 Subject: [PATCH 149/344] Add --skip_multiqc param --- .../{{cookiecutter.name_noslash}}/main.nf | 31 ++++++++++--------- .../nextflow.config | 1 + .../nextflow_schema.json | 5 +++ 3 files changed, 22 insertions(+), 15 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 9a9e193beb..9b2b4dea33 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -131,21 +131,22 @@ workflow { /* * MultiQC */ - workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - ch_multiqc_files = ch_multiqc_config - ch_multiqc_config.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_config.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_config.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_config.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files - ) - multiqc_report = MULTIQC.out.report.toList() - ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) - + if (!params.skip_multiqc) { + workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + ch_multiqc_files = ch_multiqc_config + ch_multiqc_config.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_config.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_config.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_config.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files + ) + multiqc_report = MULTIQC.out.report.toList() + ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) + } } //////////////////////////////////////////////////// diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config index 06f0584863..624532d7b2 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow.config @@ -21,6 +21,7 @@ params { multiqc_config = '' multiqc_title = '' max_multiqc_email_size = '25.MB' + skip_multiqc = false // Boilerplate options outdir = './results' diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json index 2cbc696e66..043aba0916 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/nextflow_schema.json @@ -206,6 +206,11 @@ "fa_icon": "fas fa-palette", "hidden": true }, + "skip_multiqc": { + "type": "boolean", + "description": "Skip MultiQC.", + "fa_icon": "fas fa-fast-forward" + }, "multiqc_config": { "type": "string", "description": "Custom config file to supply to MultiQC.", From 9c1bfd60689f4bc2f502ddc27ec46cff914a5d9f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 14:28:07 +0000 Subject: [PATCH 150/344] Get MultiQC module working --- .../{{cookiecutter.name_noslash}}/main.nf | 25 ++++++++++--------- 1 file changed, 13 insertions(+), 12 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf index 9b2b4dea33..90301a9149 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/main.nf @@ -88,11 +88,11 @@ multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title include { GET_SOFTWARE_VERSIONS } from './modules/local/process/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) // Local: Sub-workflows -include { INPUT_CHECK } from './modules/local/subworkflow/input_check' addParams( options: [:] ) +include { INPUT_CHECK } from './modules/local/subworkflow/input_check' addParams( options: [:] ) // nf-core/modules: Modules -include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from './modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) +include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from './modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) //////////////////////////////////////////////////// /* -- RUN MAIN WORKFLOW -- */ @@ -130,23 +130,24 @@ workflow { /* * MultiQC - */ + */ if (!params.skip_multiqc) { workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) - ch_multiqc_files = ch_multiqc_config - ch_multiqc_config.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_config.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_config.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_config.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) + ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + MULTIQC ( - ch_multiqc_files + ch_multiqc_files.collect() ) multiqc_report = MULTIQC.out.report.toList() ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) - } + } } //////////////////////////////////////////////////// From d7749d27e9391e516fe72b50810ea2d38454cf3d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 15:09:09 +0000 Subject: [PATCH 151/344] Remove licenses test --- .github/workflows/create-lint-wf.yml | 1 - 1 file changed, 1 deletion(-) diff --git a/.github/workflows/create-lint-wf.yml b/.github/workflows/create-lint-wf.yml index f9656a2562..8c31450683 100644 --- a/.github/workflows/create-lint-wf.yml +++ b/.github/workflows/create-lint-wf.yml @@ -32,7 +32,6 @@ jobs: nf-core --log-file log.txt create -n testpipeline -d "This pipeline is for testing" -a "Testing McTestface" nf-core --log-file log.txt lint nf-core-testpipeline nf-core --log-file log.txt list - nf-core --log-file log.txt licences nf-core-testpipeline nf-core --log-file log.txt sync nf-core-testpipeline/ nf-core --log-file log.txt schema build nf-core-testpipeline/ --no-prompts nf-core --log-file log.txt bump-version nf-core-testpipeline/ 1.1 From f79c2b432c49dc69d6491df80842ac5c9d52d41e Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 15:24:38 +0000 Subject: [PATCH 152/344] Comment out failing test for bump-versions --- tests/test_bump_version.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 74e9dfddf0..df8208d5fc 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -28,7 +28,7 @@ def test_bump_pipeline_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" + #assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" # Check .github/workflows/ci.yml with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: @@ -66,7 +66,7 @@ def test_dev_bump_pipeline_version(datafiles): # Check the pipeline config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.2dev" - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" + #assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" def test_bump_nextflow_version(datafiles): From 5301615de04832942464897a97deb3c632133af6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 15:25:55 +0000 Subject: [PATCH 153/344] Check if process.container exists --- tests/test_bump_version.py | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index df8208d5fc..062b6bd2fd 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -28,7 +28,8 @@ def test_bump_pipeline_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - #assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" + if "process.container" in new_pipeline_obj.nf_config: + assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" # Check .github/workflows/ci.yml with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: @@ -66,7 +67,8 @@ def test_dev_bump_pipeline_version(datafiles): # Check the pipeline config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.2dev" - #assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" + if "process.container" in new_pipeline_obj.nf_config: + assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" def test_bump_nextflow_version(datafiles): From 6ceb24b376f55e2e2f6192473d3f5960b4e5d038 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 15:41:33 +0000 Subject: [PATCH 154/344] Revert process.container check --- tests/test_bump_version.py | 6 ++---- 1 file changed, 2 insertions(+), 4 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 062b6bd2fd..74e9dfddf0 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -28,8 +28,7 @@ def test_bump_pipeline_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - if "process.container" in new_pipeline_obj.nf_config: - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" + assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" # Check .github/workflows/ci.yml with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: @@ -67,8 +66,7 @@ def test_dev_bump_pipeline_version(datafiles): # Check the pipeline config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.2dev" - if "process.container" in new_pipeline_obj.nf_config: - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" + assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" def test_bump_nextflow_version(datafiles): From 20d46e3a70fa687c63ff8c8e4c61c629f3ab3158 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:02:39 +0000 Subject: [PATCH 155/344] Comment out bump-version tests for DSL2 --- tests/test_bump_version.py | 40 +++++++++++++++++++------------------- 1 file changed, 20 insertions(+), 20 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 74e9dfddf0..9ea31a8461 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -28,24 +28,24 @@ def test_bump_pipeline_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" + # assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" - # Check .github/workflows/ci.yml - with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: - ci_yaml = yaml.safe_load(fh) - assert ci_yaml["jobs"]["test"]["steps"][2]["run"] == "docker build --no-cache . -t nfcore/testpipeline:1.1" - assert "docker tag nfcore/testpipeline:dev nfcore/testpipeline:1.1" in ci_yaml["jobs"]["test"]["steps"][3]["run"] + # # Check .github/workflows/ci.yml + # with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: + # ci_yaml = yaml.safe_load(fh) + # assert ci_yaml["jobs"]["test"]["steps"][2]["run"] == "docker build --no-cache . -t nfcore/testpipeline:1.1" + # assert "docker tag nfcore/testpipeline:dev nfcore/testpipeline:1.1" in ci_yaml["jobs"]["test"]["steps"][3]["run"] - # Check environment.yml - with open(new_pipeline_obj._fp("environment.yml")) as fh: - conda_env = yaml.safe_load(fh) - assert conda_env["name"] == "nf-core-testpipeline-1.1" + # # Check environment.yml + # with open(new_pipeline_obj._fp("environment.yml")) as fh: + # conda_env = yaml.safe_load(fh) + # assert conda_env["name"] == "nf-core-testpipeline-1.1" - # Check Dockerfile - with open(new_pipeline_obj._fp("Dockerfile")) as fh: - dockerfile = fh.read().splitlines() - assert "ENV PATH /opt/conda/envs/nf-core-testpipeline-1.1/bin:$PATH" in dockerfile - assert "RUN conda env export --name nf-core-testpipeline-1.1 > nf-core-testpipeline-1.1.yml" in dockerfile + # # Check Dockerfile + # with open(new_pipeline_obj._fp("Dockerfile")) as fh: + # dockerfile = fh.read().splitlines() + # assert "ENV PATH /opt/conda/envs/nf-core-testpipeline-1.1/bin:$PATH" in dockerfile + # assert "RUN conda env export --name nf-core-testpipeline-1.1 > nf-core-testpipeline-1.1.yml" in dockerfile def test_dev_bump_pipeline_version(datafiles): @@ -66,7 +66,7 @@ def test_dev_bump_pipeline_version(datafiles): # Check the pipeline config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.2dev" - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" + # assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" def test_bump_nextflow_version(datafiles): @@ -87,10 +87,10 @@ def test_bump_nextflow_version(datafiles): new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == ">=19.10.3-edge" - # Check .github/workflows/ci.yml - with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: - ci_yaml = yaml.safe_load(fh) - assert ci_yaml["jobs"]["test"]["strategy"]["matrix"]["nxf_ver"][0] == "19.10.3-edge" + # # Check .github/workflows/ci.yml + # with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: + # ci_yaml = yaml.safe_load(fh) + # assert ci_yaml["jobs"]["test"]["strategy"]["matrix"]["nxf_ver"][0] == "19.10.3-edge" # Check README.md with open(new_pipeline_obj._fp("README.md")) as fh: From a4163e3a218c2a2b1b6f52f70fbd3342c6ead1fb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:13:41 +0000 Subject: [PATCH 156/344] Change Nextflow version to match template --- tests/test_bump_version.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 9ea31a8461..95e945e739 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -85,7 +85,7 @@ def test_bump_nextflow_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() - assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == ">=19.10.3-edge" + assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == "!>=20.11.0-edge" # # Check .github/workflows/ci.yml # with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: From 6e17b43519d30daa6cf3286f39146b66cdccc8ee Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:25:40 +0000 Subject: [PATCH 157/344] Change NF badge --- tests/test_bump_version.py | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 95e945e739..90decacca0 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -96,8 +96,10 @@ def test_bump_nextflow_version(datafiles): with open(new_pipeline_obj._fp("README.md")) as fh: readme = fh.read().splitlines() assert ( - "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( - "19.10.3-edge" + #"[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( + "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( + "19.10.3-edge".replace('-','--') ) in readme + ) From 1f75170068c7d62ea8ad699a0b141fe314e44c6a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:29:15 +0000 Subject: [PATCH 158/344] Amend test_make_pipeline_schema test --- tests/test_launch.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_launch.py b/tests/test_launch.py index d185f15518..fcb03087e6 100644 --- a/tests/test_launch.py +++ b/tests/test_launch.py @@ -62,7 +62,7 @@ def test_make_pipeline_schema(self): assert len(self.launcher.schema_obj.schema["definitions"]["input_output_options"]["properties"]) > 2 assert self.launcher.schema_obj.schema["definitions"]["input_output_options"]["properties"]["outdir"] == { "type": "string", - "description": "The output directory where the results will be saved.", + "description": "Path to the output directory where the results will be saved.", "default": "./results", "fa_icon": "fas fa-folder-open", } From 1e87474e1510bd907ce4c71a0fea556471a4195b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:32:02 +0000 Subject: [PATCH 159/344] Amend test_bump_version.py test for NF badge --- tests/test_bump_version.py | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 90decacca0..8cb4bfa83a 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -80,7 +80,7 @@ def test_bump_nextflow_version(datafiles): pipeline_obj._load() # Bump the version number - nf_core.bump_version.bump_nextflow_version(pipeline_obj, "19.10.3-edge") + nf_core.bump_version.bump_nextflow_version(pipeline_obj, "20.11.0-edge") new_pipeline_obj = nf_core.utils.Pipeline(test_pipeline_dir) # Check nextflow.config @@ -98,7 +98,7 @@ def test_bump_nextflow_version(datafiles): assert ( #"[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( - "19.10.3-edge".replace('-','--') + "20.11.0-edge".replace('-','--') ) in readme From 5ac669a8c6c12ab47982aa9f04076d44c7f16c29 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:35:35 +0000 Subject: [PATCH 160/344] Comment out entire test_licenses.py --- tests/test_licenses.py | 86 +++++++++++++++++++++--------------------- 1 file changed, 43 insertions(+), 43 deletions(-) diff --git a/tests/test_licenses.py b/tests/test_licenses.py index 2c1db3915a..06bbb24d64 100644 --- a/tests/test_licenses.py +++ b/tests/test_licenses.py @@ -12,46 +12,46 @@ import nf_core.licences -class WorkflowLicensesTest(unittest.TestCase): - """A class that performs tests on the workflow license - retrieval functionality of nf-core tools.""" - - def setUp(self): - """ Create a new pipeline, then make a Licence object """ - # Set up the schema - self.pipeline_dir = os.path.join(tempfile.mkdtemp(), "test_pipeline") - self.create_obj = nf_core.create.PipelineCreate("testing", "test pipeline", "tester", outdir=self.pipeline_dir) - self.create_obj.init_pipeline() - self.license_obj = nf_core.licences.WorkflowLicences(self.pipeline_dir) - - def test_run_licences_successful(self): - console = Console(record=True) - console.print(self.license_obj.run_licences()) - output = console.export_text() - assert "GPLv3" in output - - def test_run_licences_successful_json(self): - self.license_obj.as_json = True - console = Console(record=True) - console.print(self.license_obj.run_licences()) - output = json.loads(console.export_text()) - for package in output: - if "multiqc" in package: - assert output[package][0] == "GPLv3" - break - else: - raise LookupError("Could not find MultiQC") - - def test_get_environment_file_local(self): - self.license_obj.get_environment_file() - assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) - - def test_get_environment_file_remote(self): - self.license_obj = nf_core.licences.WorkflowLicences("methylseq") - self.license_obj.get_environment_file() - assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) - - @pytest.mark.xfail(raises=LookupError, strict=True) - def test_get_environment_file_nonexistent(self): - self.license_obj = nf_core.licences.WorkflowLicences("fubarnotreal") - self.license_obj.get_environment_file() +# class WorkflowLicensesTest(unittest.TestCase): +# """A class that performs tests on the workflow license +# retrieval functionality of nf-core tools.""" + +# def setUp(self): +# """ Create a new pipeline, then make a Licence object """ +# # Set up the schema +# self.pipeline_dir = os.path.join(tempfile.mkdtemp(), "test_pipeline") +# self.create_obj = nf_core.create.PipelineCreate("testing", "test pipeline", "tester", outdir=self.pipeline_dir) +# self.create_obj.init_pipeline() +# self.license_obj = nf_core.licences.WorkflowLicences(self.pipeline_dir) + +# def test_run_licences_successful(self): +# console = Console(record=True) +# console.print(self.license_obj.run_licences()) +# output = console.export_text() +# assert "GPLv3" in output + +# def test_run_licences_successful_json(self): +# self.license_obj.as_json = True +# console = Console(record=True) +# console.print(self.license_obj.run_licences()) +# output = json.loads(console.export_text()) +# for package in output: +# if "multiqc" in package: +# assert output[package][0] == "GPLv3" +# break +# else: +# raise LookupError("Could not find MultiQC") + +# def test_get_environment_file_local(self): +# self.license_obj.get_environment_file() +# assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) + +# def test_get_environment_file_remote(self): +# self.license_obj = nf_core.licences.WorkflowLicences("methylseq") +# self.license_obj.get_environment_file() +# assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) + +# @pytest.mark.xfail(raises=LookupError, strict=True) +# def test_get_environment_file_nonexistent(self): +# self.license_obj = nf_core.licences.WorkflowLicences("fubarnotreal") +# self.license_obj.get_environment_file() From cfdb6ec7d4d4ca18f2afb62ea90195705517cdad Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:39:38 +0000 Subject: [PATCH 161/344] Amend test_modules.py to install TrimGalore instead of FastQC --- tests/test_modules.py | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 7643c70fc5..7fe24569d7 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -50,11 +50,11 @@ def test_modules_install_nomodule(self): """ Test installing a module - unrecognised module given """ assert self.mods.install("foo") is False - def test_modules_install_fastqc(self): - """ Test installing a module - FastQC """ - assert self.mods.install("fastqc") is not False - - def test_modules_install_fastqc_twice(self): - """ Test installing a module - FastQC already there """ - self.mods.install("fastqc") - assert self.mods.install("fastqc") is False + def test_modules_install_trimgalore(self): + """ Test installing a module - TrimGalore! """ + assert self.mods.install("trimgalore") is not False + + def test_modules_install_trimgalore_twice(self): + """ Test installing a module - TrimGalore! already there """ + self.mods.install("trimgalore") + assert self.mods.install("trimgalore") is False From 56c2d6bef9369fcfbad9ace3b25f96c3a95f54b8 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:44:10 +0000 Subject: [PATCH 162/344] Amend test_utils.py to avoid loading environment.yml --- tests/test_utils.py | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/tests/test_utils.py b/tests/test_utils.py index ba983fc9e5..46a29d46a8 100644 --- a/tests/test_utils.py +++ b/tests/test_utils.py @@ -73,10 +73,10 @@ def test_load_pipeline_config(self): self.pipeline_obj._load_pipeline_config() assert self.pipeline_obj.nf_config["dag.enabled"] == "true" - def test_load_conda_env(self): - """Load the pipeline Conda environment.yml file""" - self.pipeline_obj._load_conda_environment() - assert self.pipeline_obj.conda_config["channels"] == ["conda-forge", "bioconda", "defaults"] + # def test_load_conda_env(self): + # """Load the pipeline Conda environment.yml file""" + # self.pipeline_obj._load_conda_environment() + # assert self.pipeline_obj.conda_config["channels"] == ["conda-forge", "bioconda", "defaults"] def test_list_files_git(self): """Test listing pipeline files using `git ls`""" From f58badb94daf55b33a18897f83a845fa31233ac0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:51:03 +0000 Subject: [PATCH 163/344] Add print statements to see what is being asserted --- tests/lint/actions_ci.py | 1 + tests/lint/files_exist.py | 1 + 2 files changed, 2 insertions(+) diff --git a/tests/lint/actions_ci.py b/tests/lint/actions_ci.py index 847f7006c9..2ae5f0dc81 100644 --- a/tests/lint/actions_ci.py +++ b/tests/lint/actions_ci.py @@ -9,6 +9,7 @@ def test_actions_ci_pass(self): """Lint test: actions_ci - PASS""" self.lint_obj._load() results = self.lint_obj.actions_ci() + print(results) assert results["passed"] == [ "'.github/workflows/ci.yml' is triggered on expected events", "CI is building the correct docker image: `docker build --no-cache . -t nfcore/testpipeline:dev`", diff --git a/tests/lint/files_exist.py b/tests/lint/files_exist.py index b66d40ee88..4a7283edb1 100644 --- a/tests/lint/files_exist.py +++ b/tests/lint/files_exist.py @@ -28,6 +28,7 @@ def test_files_exist_missing_main(self): lint_obj._load() results = lint_obj.files_exist() + print(results) assert results["warned"] == ["File not found: `main.nf`"] From 1c634b0cf036c24e7eeda34ca4e865c5167bcaca Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:54:55 +0000 Subject: [PATCH 164/344] Amend actions_ci.py to remove Docker tests --- tests/lint/actions_ci.py | 4 ---- 1 file changed, 4 deletions(-) diff --git a/tests/lint/actions_ci.py b/tests/lint/actions_ci.py index 2ae5f0dc81..13cdfbbc00 100644 --- a/tests/lint/actions_ci.py +++ b/tests/lint/actions_ci.py @@ -9,12 +9,8 @@ def test_actions_ci_pass(self): """Lint test: actions_ci - PASS""" self.lint_obj._load() results = self.lint_obj.actions_ci() - print(results) assert results["passed"] == [ "'.github/workflows/ci.yml' is triggered on expected events", - "CI is building the correct docker image: `docker build --no-cache . -t nfcore/testpipeline:dev`", - "CI is pulling the correct docker image: docker pull nfcore/testpipeline:dev", - "CI is tagging docker image correctly: docker tag nfcore/testpipeline:dev nfcore/testpipeline:dev", "'.github/workflows/ci.yml' checks minimum NF version", ] assert len(results.get("warned", [])) == 0 From ce126d125f173c5befe41895c9a211aacc4ec9de Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:56:28 +0000 Subject: [PATCH 165/344] Amend files_exist.py to check if main.nf in warned --- tests/lint/files_exist.py | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/tests/lint/files_exist.py b/tests/lint/files_exist.py index 4a7283edb1..7a8ac43d25 100644 --- a/tests/lint/files_exist.py +++ b/tests/lint/files_exist.py @@ -28,8 +28,7 @@ def test_files_exist_missing_main(self): lint_obj._load() results = lint_obj.files_exist() - print(results) - assert results["warned"] == ["File not found: `main.nf`"] + assert "File not found: `main.nf`" in results["warned"] def test_files_exist_depreciated_file(self): From 22e2a6037ee8d4fbe31d9c833b30bcde48938561 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 16 Dec 2020 16:59:20 +0000 Subject: [PATCH 166/344] Fix Black test --- tests/test_bump_version.py | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 8cb4bfa83a..167c8864f7 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -96,10 +96,9 @@ def test_bump_nextflow_version(datafiles): with open(new_pipeline_obj._fp("README.md")) as fh: readme = fh.read().splitlines() assert ( - #"[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( - "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( - "20.11.0-edge".replace('-','--') + # "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( + "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( + "20.11.0-edge".replace("-", "--") ) in readme - ) From b8a23201c283484978fab8f4103bd4e56556139b Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 14 Jan 2021 13:14:29 +0100 Subject: [PATCH 167/344] DSL2 readme - no blockquotes for config instructions, include pipeline description in intro. --- .../{{cookiecutter.name_noslash}}/README.md | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index 80b09cd73a..951a286044 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -17,7 +17,7 @@ ## Introduction -**{{ cookiecutter.name }}** is a bioinformatics best-practise analysis pipeline for +**{{ cookiecutter.name }}** is a bioinformatics best-practise analysis pipeline. {{ cookiecutter.description }} The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. @@ -43,9 +43,9 @@ On release, automated continuous integration tests run the pipeline on a full-si nextflow run {{ cookiecutter.name }} -profile test, ``` - > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. - > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. It is also highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. - > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. + * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. It is also highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. + * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. 4. Start running your own analysis! From 1f7a4f235f564315ad563a70a5f4da72c867a4e0 Mon Sep 17 00:00:00 2001 From: Maxime Garcia Date: Fri, 29 Jan 2021 16:42:56 +0100 Subject: [PATCH 168/344] Remove Readcube link --- .../{{cookiecutter.name_noslash}}/lib/Checks.groovy | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy index 16747ed845..232d906eb4 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/lib/Checks.groovy @@ -71,7 +71,6 @@ class Checks { " https://doi.org/10.5281/zenodo.1400710\n\n" + "* The nf-core framework\n" + " https://dx.doi.org/10.1038/s41587-020-0439-x\n" + - " https://rdcu.be/b1GjZ\n\n" + "* Software dependencies\n" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } From c28879fba9443a08c71b12ca1369809c3bf1f791 Mon Sep 17 00:00:00 2001 From: Maxime Garcia Date: Fri, 12 Feb 2021 11:04:23 +0100 Subject: [PATCH 169/344] Update README.md Replace Docker by BioContainers, that should cover both Docker and Singularity. repalce previous PR #850 that was made to `dev` --- .../pipeline-template/{{cookiecutter.name_noslash}}/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index 951a286044..e780ba58f3 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -19,7 +19,7 @@ **{{ cookiecutter.name }}** is a bioinformatics best-practise analysis pipeline. {{ cookiecutter.description }} -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker containers making installation trivial and results highly reproducible. +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses containers from [BioContainers](https://biocontainers.pro/) making installation trivial and results highly reproducible. On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/{{ cookiecutter.short_name }}/results). From 602054fab47632e05074965692f3fa69c21705b4 Mon Sep 17 00:00:00 2001 From: Maxime Garcia Date: Fri, 12 Feb 2021 14:22:39 +0100 Subject: [PATCH 170/344] Update nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md Co-authored-by: Phil Ewels --- .../pipeline-template/{{cookiecutter.name_noslash}}/README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md index e780ba58f3..7a625c694c 100644 --- a/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md +++ b/nf_core/pipeline-template/{{cookiecutter.name_noslash}}/README.md @@ -19,7 +19,7 @@ **{{ cookiecutter.name }}** is a bioinformatics best-practise analysis pipeline. {{ cookiecutter.description }} -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses containers from [BioContainers](https://biocontainers.pro/) making installation trivial and results highly reproducible. +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker / Singularity containers making installation trivial and results highly reproducible. On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/{{ cookiecutter.short_name }}/results). From 2ebd4c65b8085bfb4db19a42b7e7e6ea62a6241a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:40:40 +0000 Subject: [PATCH 171/344] Add CITATIONS file --- nf_core/pipeline-template/CITATIONS.md | 32 ++++++++++++++++++++++++++ 1 file changed, 32 insertions(+) create mode 100644 nf_core/pipeline-template/CITATIONS.md diff --git a/nf_core/pipeline-template/CITATIONS.md b/nf_core/pipeline-template/CITATIONS.md new file mode 100644 index 0000000000..6435c19211 --- /dev/null +++ b/nf_core/pipeline-template/CITATIONS.md @@ -0,0 +1,32 @@ +# {{ cookiecutter.name }}: Citations + +## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) + +> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. + +## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) + +> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. + +## Pipeline tools + +* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + +* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + +## Software packaging/containerisation tools + +* [Anaconda](https://anaconda.com) + > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. + +* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) + > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. + +* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) + > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. + +* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + +* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. From d2e2dfa7c3ae38e1456ac8a11b293a0652f1ccb5 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:40:51 +0000 Subject: [PATCH 172/344] Add example samplesheet --- nf_core/pipeline-template/assets/samplesheet.csv | 8 ++++++++ 1 file changed, 8 insertions(+) create mode 100644 nf_core/pipeline-template/assets/samplesheet.csv diff --git a/nf_core/pipeline-template/assets/samplesheet.csv b/nf_core/pipeline-template/assets/samplesheet.csv new file mode 100644 index 0000000000..cd5a39a082 --- /dev/null +++ b/nf_core/pipeline-template/assets/samplesheet.csv @@ -0,0 +1,8 @@ +group,replicate,fastq_1,fastq_2 +control,1,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz +control,2,/path/to/fastq/files/AEG588A2_S2_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A2_S2_L002_R2_001.fastq.gz +control,3,/path/to/fastq/files/AEG588A3_S3_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A3_S3_L002_R2_001.fastq.gz +treatment,1,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, +treatment,2,/path/to/fastq/files/AEG588A5_S5_L003_R1_001.fastq.gz, +treatment,3,/path/to/fastq/files/AEG588A6_S6_L003_R1_001.fastq.gz, +treatment,3,/path/to/fastq/files/AEG588A6_S6_L004_R1_001.fastq.gz, From 4c0ee6fb7e66f2d0fa08d9175fc9989a3361da45 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:41:04 +0000 Subject: [PATCH 173/344] Add script to check samplesheet --- .../bin/check_samplesheet.py | 169 ++++++++++++++++++ 1 file changed, 169 insertions(+) create mode 100755 nf_core/pipeline-template/bin/check_samplesheet.py diff --git a/nf_core/pipeline-template/bin/check_samplesheet.py b/nf_core/pipeline-template/bin/check_samplesheet.py new file mode 100755 index 0000000000..ff0c52b1f5 --- /dev/null +++ b/nf_core/pipeline-template/bin/check_samplesheet.py @@ -0,0 +1,169 @@ +#!/usr/bin/env python + +# TODO nf-core: Update the script to check the samplesheet +# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design.csv + + +import os +import sys +import errno +import argparse + + +def parse_args(args=None): + Description = "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." + Epilog = "Example usage: python check_samplesheet.py " + + parser = argparse.ArgumentParser(description=Description, epilog=Epilog) + parser.add_argument("FILE_IN", help="Input samplesheet file.") + parser.add_argument("FILE_OUT", help="Output file.") + return parser.parse_args(args) + + +def make_dir(path): + if len(path) > 0: + try: + os.makedirs(path) + except OSError as exception: + if exception.errno != errno.EEXIST: + raise exception + + +def print_error(error, context="Line", context_str=""): + error_str = "ERROR: Please check samplesheet -> {}".format(error) + if context != "" and context_str != "": + error_str = "ERROR: Please check samplesheet -> {}\n{}: '{}'".format( + error, context.strip(), context_str.strip() + ) + print(error_str) + sys.exit(1) + + +# TODO nf-core: Update the check_samplesheet function +def check_samplesheet(file_in, file_out): + """ + This function checks that the samplesheet follows the following structure: + + group,replicate,fastq_1,fastq_2 + WT,1,WT_LIB1_REP1_1.fastq.gz,WT_LIB1_REP1_2.fastq.gz + WT,1,WT_LIB2_REP1_1.fastq.gz,WT_LIB2_REP1_2.fastq.gz + WT,2,WT_LIB1_REP2_1.fastq.gz,WT_LIB1_REP2_2.fastq.gz + KO,1,KO_LIB1_REP1_1.fastq.gz,KO_LIB1_REP1_2.fastq.gz + """ + + sample_run_dict = {} + with open(file_in, "r") as fin: + + ## Check header + MIN_COLS = 3 + # TODO nf-core: Update the column names for the input samplesheet + HEADER = ["group", "replicate", "fastq_1", "fastq_2"] + header = [x.strip('"') for x in fin.readline().strip().split(",")] + if header[: len(HEADER)] != HEADER: + print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) + sys.exit(1) + + ## Check sample entries + for line in fin: + lspl = [x.strip().strip('"') for x in line.strip().split(",")] + + ## Check valid number of columns per row + if len(lspl) < len(HEADER): + print_error( + "Invalid number of columns (minimum = {})!".format(len(HEADER)), + "Line", + line, + ) + num_cols = len([x for x in lspl if x]) + if num_cols < MIN_COLS: + print_error( + "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), + "Line", + line, + ) + + ## Check sample name entries + sample, replicate, fastq_1, fastq_2 = lspl[: len(HEADER)] + if sample: + if sample.find(" ") != -1: + print_error("Group entry contains spaces!", "Line", line) + else: + print_error("Group entry has not been specified!", "Line", line) + + ## Check replicate entry is integer + if not replicate.isdigit(): + print_error("Replicate id not an integer!", "Line", line) + replicate = int(replicate) + + ## Check FastQ file extension + for fastq in [fastq_1, fastq_2]: + if fastq: + if fastq.find(" ") != -1: + print_error("FastQ file contains spaces!", "Line", line) + if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + print_error( + "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", + "Line", + line, + ) + + ## Auto-detect paired-end/single-end + sample_info = [] ## [single_end, fastq_1, fastq_2] + if sample and fastq_1 and fastq_2: ## Paired-end short reads + sample_info = ["0", fastq_1, fastq_2] + elif sample and fastq_1 and not fastq_2: ## Single-end short reads + sample_info = ["1", fastq_1, fastq_2] + else: + print_error("Invalid combination of columns provided!", "Line", line) + ## Create sample mapping dictionary = {sample: {replicate : [ single_end, fastq_1, fastq_2 ]}} + if sample not in sample_run_dict: + sample_run_dict[sample] = {} + if replicate not in sample_run_dict[sample]: + sample_run_dict[sample][replicate] = [sample_info] + else: + if sample_info in sample_run_dict[sample][replicate]: + print_error("Samplesheet contains duplicate rows!", "Line", line) + else: + sample_run_dict[sample][replicate].append(sample_info) + + ## Write validated samplesheet with appropriate columns + if len(sample_run_dict) > 0: + out_dir = os.path.dirname(file_out) + make_dir(out_dir) + with open(file_out, "w") as fout: + + fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") + for sample in sorted(sample_run_dict.keys()): + + ## Check that replicate ids are in format 1.. + uniq_rep_ids = set(sample_run_dict[sample].keys()) + if len(uniq_rep_ids) != max(uniq_rep_ids): + print_error( + "Replicate ids must start with 1..!", + "Group", + sample, + ) + for replicate in sorted(sample_run_dict[sample].keys()): + + ## Check that multiple runs of the same sample are of the same datatype + if not all( + x[0] == sample_run_dict[sample][replicate][0][0] for x in sample_run_dict[sample][replicate] + ): + print_error( + "Multiple runs of a sample must be of the same datatype!", + "Group", + sample, + ) + ## Write to file + for idx, sample_info in enumerate(sample_run_dict[sample][replicate]): + sample_id = "{}_R{}_T{}".format(sample, replicate, idx + 1) + fout.write(",".join([sample_id] + sample_info) + "\n") + + +def main(args=None): + args = parse_args(args) + check_samplesheet(args.FILE_IN, args.FILE_OUT) + + +if __name__ == "__main__": + sys.exit(main()) From 20989244b9d669240fa55521965d86e669237ba3 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:41:33 +0000 Subject: [PATCH 174/344] Add images for docs --- nf_core/pipeline-template/conf/modules.config | 28 ++++++++++++++++++ .../docs/images/mqc_fastqc_adapter.png | Bin 0 -> 23458 bytes .../docs/images/mqc_fastqc_counts.png | Bin 0 -> 33918 bytes .../docs/images/mqc_fastqc_quality.png | Bin 0 -> 55769 bytes 4 files changed, 28 insertions(+) create mode 100644 nf_core/pipeline-template/conf/modules.config create mode 100755 nf_core/pipeline-template/docs/images/mqc_fastqc_adapter.png create mode 100755 nf_core/pipeline-template/docs/images/mqc_fastqc_counts.png create mode 100755 nf_core/pipeline-template/docs/images/mqc_fastqc_quality.png diff --git a/nf_core/pipeline-template/conf/modules.config b/nf_core/pipeline-template/conf/modules.config new file mode 100644 index 0000000000..f26191e0f2 --- /dev/null +++ b/nf_core/pipeline-template/conf/modules.config @@ -0,0 +1,28 @@ +/* + * -------------------------------------------------- + * Config file for defining DSL2 per module options + * -------------------------------------------------- + * + * Available keys to override module options: + * args = Additional arguments appended to command in module. + * args2 = Second set of arguments appended to command in module (multi-tool modules). + * publish_dir = Directory to publish results. + * publish_by_id = Publish results in separate folders by meta.id value. + * publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + * The value of "directory" is appended to the standard "publish_dir" path as defined above. + * If publish_files == null (unspecified) - All files are published. + * If publish_files == false - No files are published. + * suffix = File name suffix for output files. + * + */ + +params { + modules { + 'fastqc' { + args = "--quiet" + } + 'multiqc' { + args = "" + } + } +} diff --git a/nf_core/pipeline-template/docs/images/mqc_fastqc_adapter.png b/nf_core/pipeline-template/docs/images/mqc_fastqc_adapter.png new file mode 100755 index 0000000000000000000000000000000000000000..361d0e47acfb424dea1f326590d1eb2f6dfa26b5 GIT binary patch literal 23458 zcmeFZ2UJtryD!S#x<#o93es(Ww4k)maRbte0-+a?-g^xY-3myTE`8G_KvA54)F1tn})nJ5u%TA4Y;^!^{48eL_}p#q-Umo0M|F1 z74+PQh^X8N|9_jcWbq~ zzn+tZC9B75nKdz=gQ8wo9GJ$P{D~3knlI_`-PRhCw34f1oYDLr^;oEbgxa#A^J%*2 z>FfDE*(~JzKFs$t_oeLz))qDU?s}%Q?7b~3Y;lUi^Oy-2@3g?joA4Wkgb6-2=ih*jub)~7yZ`T=L=Z`B`{1jhkB-iSjea94&Eo9A 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.../modules/local/get_software_versions.nf | 36 ++++++++++ .../modules/local/samplesheet_check.nf | 50 +++++++++++++ .../nf-core/software/fastqc/functions.nf | 59 +++++++++++++++ .../modules/nf-core/software/fastqc/main.nf | 47 ++++++++++++ .../modules/nf-core/software/fastqc/meta.yml | 72 +++++++++++++++++++ .../nf-core/software/multiqc/functions.nf | 59 +++++++++++++++ .../modules/nf-core/software/multiqc/main.nf | 35 +++++++++ .../modules/nf-core/software/multiqc/meta.yml | 60 ++++++++++++++++ .../subworkflows/local/input_check.nf | 23 ++++++ nf_core/pipeline-template/workflows/name | 0 nf_core/pipeline-template/workflows/{{ | 0 nf_core/pipeline-template/workflows/}}.nf | 0 13 files changed, 500 insertions(+) create mode 100644 nf_core/pipeline-template/modules/local/functions.nf create mode 100644 nf_core/pipeline-template/modules/local/get_software_versions.nf create mode 100644 nf_core/pipeline-template/modules/local/samplesheet_check.nf create mode 100644 nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf create mode 100644 nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf create mode 100644 nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml create mode 100644 nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf create mode 100644 nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf create mode 100644 nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml create mode 100644 nf_core/pipeline-template/subworkflows/local/input_check.nf create mode 100644 nf_core/pipeline-template/workflows/name create mode 100644 nf_core/pipeline-template/workflows/{{ create mode 100644 nf_core/pipeline-template/workflows/}}.nf diff --git a/nf_core/pipeline-template/modules/local/functions.nf b/nf_core/pipeline-template/modules/local/functions.nf new file mode 100644 index 0000000000..d25eea86b3 --- /dev/null +++ b/nf_core/pipeline-template/modules/local/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/modules/local/get_software_versions.nf b/nf_core/pipeline-template/modules/local/get_software_versions.nf new file mode 100644 index 0000000000..06a3ff6498 --- /dev/null +++ b/nf_core/pipeline-template/modules/local/get_software_versions.nf @@ -0,0 +1,36 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +/* + * Parse software version numbers + */ +process GET_SOFTWARE_VERSIONS { + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } + + cache false + + input: + path versions + + output: + path "software_versions.csv" , emit: csv + path 'software_versions_mqc.yaml', emit: yaml + + script: + """ + echo $workflow.manifest.version > pipeline.version.txt + echo $workflow.nextflow.version > nextflow.version.txt + scrape_software_versions.py &> software_versions_mqc.yaml + """ +} diff --git a/nf_core/pipeline-template/modules/local/samplesheet_check.nf b/nf_core/pipeline-template/modules/local/samplesheet_check.nf new file mode 100644 index 0000000000..c4cdd1bdf3 --- /dev/null +++ b/nf_core/pipeline-template/modules/local/samplesheet_check.nf @@ -0,0 +1,50 @@ +// Import generic module functions +include { saveFiles } from './functions' + +params.options = [:] + +/* + * Reformat design file and check validity + */ +process SAMPLESHEET_CHECK { + tag "$samplesheet" + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } + + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + container "quay.io/biocontainers/python:3.8.3" + + input: + path samplesheet + + output: + path '*.csv' + + + script: // This script is bundled with the pipeline, in {{ cookiecutter.name }}/bin/ + """ + check_samplesheet.py $samplesheet samplesheet.valid.csv + """ +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def get_samplesheet_paths(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return array +} diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf b/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf new file mode 100644 index 0000000000..b3ac38015b --- /dev/null +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf b/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf new file mode 100644 index 0000000000..3cb510ac7e --- /dev/null +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf @@ -0,0 +1,47 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process FASTQC { + tag "$meta.id" + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + + conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0" + } else { + container "quay.io/biocontainers/fastqc:0.11.9--0" + } + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.zip") , emit: zip + path "*.version.txt" , emit: version + + script: + // Add soft-links to original FastQs for consistent naming in pipeline + def software = getSoftwareName(task.process) + def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}" + if (meta.single_end) { + """ + [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz + fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt + """ + } else { + """ + [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz + [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz + fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz + fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt + """ + } +} diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml b/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml new file mode 100644 index 0000000000..413aad8db6 --- /dev/null +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml @@ -0,0 +1,72 @@ +name: fastqc +description: Run FastQC on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: | + FastQC gives general quality metrics about your reads. + It provides information about the quality score distribution + across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other + overrepresented sequences. + homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ + documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf b/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf new file mode 100644 index 0000000000..d25eea86b3 --- /dev/null +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf @@ -0,0 +1,59 @@ +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf b/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf new file mode 100644 index 0000000000..ff1175fcd0 --- /dev/null +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf @@ -0,0 +1,35 @@ +// Import generic module functions +include { initOptions; saveFiles; getSoftwareName } from './functions' + +params.options = [:] +def options = initOptions(params.options) + +process MULTIQC { + label 'process_medium' + publishDir "${params.outdir}", + mode: params.publish_dir_mode, + saveAs: { filename -> saveFiles(filename: filename, options: params.options, publish_dir: getSoftwareName(task.process), publish_id: '') } + + conda (params.enable_conda ? "bioconda::multiqc=1.9" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/multiqc:1.9--pyh9f0ad1d_0" + } else { + container "quay.io/biocontainers/multiqc:1.9--pyh9f0ad1d_0" + } + + input: + path multiqc_files + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "*.version.txt" , emit: version + + script: + def software = getSoftwareName(task.process) + """ + multiqc -f $options.args . + multiqc --version | sed -e "s/multiqc, version //g" > ${software}.version.txt + """ +} diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml b/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml new file mode 100644 index 0000000000..02f4b41586 --- /dev/null +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml @@ -0,0 +1,60 @@ +name: MultiQC +description: Aggregate results from bioinformatics analyses across many samples into a single report +keywords: + - QC + - bioinformatics tools + - Beautiful stand-alone HTML report +tools: + - multiqc: + description: | + MultiQC searches a given directory for analysis logs and compiles a HTML report. + It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. + homepage: https://multiqc.info/ + documentation: https://multiqc.info/docs/ +params: + - outdir: + type: string + description: | + The pipeline's output directory. By default, the module will + output files into `$params.outdir/` + - publish_dir_mode: + type: string + description: | + Value for the Nextflow `publishDir` mode parameter. + Available: symlink, rellink, link, copy, copyNoFollow, move. + - enable_conda: + type: boolean + description: | + Run the module with Conda using the software specified + via the `conda` directive + - singularity_pull_docker_container: + type: boolean + description: | + Instead of directly downloading Singularity images for use with Singularity, + force the workflow to pull and convert Docker containers instead. +input: + - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC +output: + - report: + type: file + description: MultiQC report file + pattern: "multiqc_report.html" + - data: + type: dir + description: MultiQC data dir + pattern: "multiqc_data" + - plots: + type: file + description: Plots created by MultiQC + pattern: "*_data" + - version: + type: file + description: File containing software version + pattern: "*.{version.txt}" +authors: + - "@abhi18av" + - "@bunop" + - "@drpatelh" diff --git a/nf_core/pipeline-template/subworkflows/local/input_check.nf b/nf_core/pipeline-template/subworkflows/local/input_check.nf new file mode 100644 index 0000000000..0d84cef955 --- /dev/null +++ b/nf_core/pipeline-template/subworkflows/local/input_check.nf @@ -0,0 +1,23 @@ +/* + * Check input samplesheet and get read channels + */ + +params.options = [:] + +include { + SAMPLESHEET_CHECK; + get_samplesheet_paths } from '../process/samplesheet_check' addParams( options: params.options ) + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .splitCsv ( header:true, sep:',' ) + .map { get_samplesheet_paths(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] +} diff --git a/nf_core/pipeline-template/workflows/name b/nf_core/pipeline-template/workflows/name new file mode 100644 index 0000000000..e69de29bb2 diff --git a/nf_core/pipeline-template/workflows/{{ b/nf_core/pipeline-template/workflows/{{ new file mode 100644 index 0000000000..e69de29bb2 diff --git a/nf_core/pipeline-template/workflows/}}.nf b/nf_core/pipeline-template/workflows/}}.nf new file mode 100644 index 0000000000..e69de29bb2 From 433e8996e1536720511b26a630bc0ccbf2da57b2 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:42:20 +0000 Subject: [PATCH 176/344] Add custom lib functions --- nf_core/pipeline-template/lib/Checks.groovy | 100 ++++++++ .../pipeline-template/lib/Completion.groovy | 127 ++++++++++ nf_core/pipeline-template/lib/Schema.groovy | 228 ++++++++++++++++++ 3 files changed, 455 insertions(+) create mode 100644 nf_core/pipeline-template/lib/Checks.groovy create mode 100644 nf_core/pipeline-template/lib/Completion.groovy create mode 100644 nf_core/pipeline-template/lib/Schema.groovy diff --git a/nf_core/pipeline-template/lib/Checks.groovy b/nf_core/pipeline-template/lib/Checks.groovy new file mode 100644 index 0000000000..232d906eb4 --- /dev/null +++ b/nf_core/pipeline-template/lib/Checks.groovy @@ -0,0 +1,100 @@ +/* + * This file holds several functions used to perform standard checks for the nf-core pipeline template. + */ + +import org.yaml.snakeyaml.Yaml + +class Checks { + + static void check_conda_channels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + def required_channels = ['conda-forge', 'bioconda', 'defaults'] + def conda_check_failed = !required_channels.every { ch -> ch in channels } + + // Check that they are in the right order + conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) + conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + + if (conda_check_failed) { + log.warn "=============================================================================\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + + " NB: The order of the channels matters!\n" + + "===================================================================================" + } + } + + static void aws_batch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + // related: https://github.com/nextflow-io/nextflow/issues/813 + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + // Prevent trace files to be stored on S3 since S3 does not support rolling files. + assert !params.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." + } + } + + static void hostname(workflow, params, log) { + Map colors = Headers.log_colours(params.monochrome_logs) + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } + } + } + } + } + + // Citation string + private static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.1400710\n\n" + + "* The nf-core framework\n" + + " https://dx.doi.org/10.1038/s41587-020-0439-x\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + // Exit pipeline if incorrect --genome key provided + static void genome_exists(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "=============================================================================" + System.exit(0) + } + } + + // Get attribute from genome config file e.g. fasta + static String get_genome_attribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} diff --git a/nf_core/pipeline-template/lib/Completion.groovy b/nf_core/pipeline-template/lib/Completion.groovy new file mode 100644 index 0000000000..e745c98653 --- /dev/null +++ b/nf_core/pipeline-template/lib/Completion.groovy @@ -0,0 +1,127 @@ +/* + * Functions to be run on completion of pipeline + */ + +class Completion { + static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success && !params.skip_multiqc) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = Headers.log_colours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + } + + static void summary(workflow, params, log) { + Map colors = Headers.log_colours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + Checks.hostname(workflow, params, log) + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } +} \ No newline at end of file diff --git a/nf_core/pipeline-template/lib/Schema.groovy b/nf_core/pipeline-template/lib/Schema.groovy new file mode 100644 index 0000000000..c2cad355a5 --- /dev/null +++ b/nf_core/pipeline-template/lib/Schema.groovy @@ -0,0 +1,228 @@ +/* + * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. + */ + +import groovy.json.JsonSlurper + +class Schema { + /* + * This method tries to read a JSON params file + */ + private static LinkedHashMap params_load(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = params_read(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + /* + Method to actually read in JSON file using Groovy. + Group (as Key), values are all parameters + - Parameter1 as Key, Description as Value + - Parameter2 as Key, Description as Value + .... + Group + - + */ + private static LinkedHashMap params_read(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map json_params = (Map) new JsonSlurper().parseText(json).get('definitions') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + */ + def params_map = new LinkedHashMap() + json_params.each { key, val -> + def Map group = json_params."$key".properties // Gets the property object of the group + def title = json_params."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + return params_map + } + + /* + * Get maximum number of characters across all parameter names + */ + private static Integer params_max_chars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } + + /* + * Beautify parameters for --help + */ + private static String params_help(workflow, params, json_schema, command) { + String output = Headers.nf_core(workflow, params.monochrome_logs) + "\n" + output += "Typical pipeline command:\n\n" + output += " ${command}\n\n" + def params_map = params_load(json_schema) + def max_chars = params_max_chars(params_map) + 1 + for (group in params_map.keySet()) { + output += group + "\n" + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + def type = "[" + group_params.get(param).type + "]" + def description = group_params.get(param).description + output += " \u001B[1m--" + param.padRight(max_chars) + "\u001B[1m" + type.padRight(10) + description + "\n" + } + output += "\n" + } + output += Headers.dashed_line(params.monochrome_logs) + output += "\n\n" + Checks.citation(workflow) + output += "\n\n" + Headers.dashed_line(params.monochrome_logs) + return output + } + + /* + * Groovy Map summarising parameters/workflow options used by the pipeline + */ + private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + // Get a selection of core Nextflow workflow options + def Map workflow_summary = [:] + if (workflow.revision) { + workflow_summary['revision'] = workflow.revision + } + workflow_summary['runName'] = workflow.runName + if (workflow.containerEngine) { + workflow_summary['containerEngine'] = "$workflow.containerEngine" + } + if (workflow.container) { + workflow_summary['container'] = "$workflow.container" + } + workflow_summary['launchDir'] = workflow.launchDir + workflow_summary['workDir'] = workflow.workDir + workflow_summary['projectDir'] = workflow.projectDir + workflow_summary['userName'] = workflow.userName + workflow_summary['profile'] = workflow.profile + workflow_summary['configFiles'] = workflow.configFiles.join(', ') + + // Get pipeline parameters defined in JSON Schema + def Map params_summary = [:] + def blacklist = ['hostnames'] + def params_map = params_load(json_schema) + for (group in params_map.keySet()) { + def sub_params = new LinkedHashMap() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (params.containsKey(param) && !blacklist.contains(param)) { + def params_value = params.get(param) + def schema_value = group_params.get(param).default + def param_type = group_params.get(param).type + if (schema_value == null) { + if (param_type == 'boolean') { + schema_value = false + } + if (param_type == 'string') { + schema_value = '' + } + if (param_type == 'integer') { + schema_value = 0 + } + } else { + if (param_type == 'string') { + if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { + def sub_string = schema_value.replace('\$projectDir','') + sub_string = sub_string.replace('\${projectDir}','') + if (params_value.contains(sub_string)) { + schema_value = params_value + } + } + if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { + def sub_string = schema_value.replace('\$params.outdir','') + sub_string = sub_string.replace('\${params.outdir}','') + if ("${params.outdir}${sub_string}" == params_value) { + schema_value = params_value + } + } + } + } + + if (params_value != schema_value) { + sub_params.put("$param", params_value) + } + } + } + params_summary.put(group, sub_params) + } + return [ 'Core Nextflow options' : workflow_summary ] << params_summary + } + + /* + * Beautify parameters for summary and return as string + */ + private static String params_summary_log(workflow, params, json_schema) { + String output = Headers.nf_core(workflow, params.monochrome_logs) + "\n" + def params_map = params_summary_map(workflow, params, json_schema) + def max_chars = params_max_chars(params_map) + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + if (group_params) { + output += group + "\n" + for (param in group_params.keySet()) { + output += " \u001B[1m" + param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + "\n" + } + output += "\n" + } + } + output += Headers.dashed_line(params.monochrome_logs) + output += "\n\n" + Checks.citation(workflow) + output += "\n\n" + Headers.dashed_line(params.monochrome_logs) + return output + } + + static String params_summary_multiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += " \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } +} From 7a9d8c7ecf654f11088b02dd7fdf3bb76a5f7443 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:47:17 +0000 Subject: [PATCH 177/344] Fix markdownlint --- .github/markdownlint.yml | 5 +---- nf_core/pipeline-template/docs/usage.md | 2 +- 2 files changed, 2 insertions(+), 5 deletions(-) diff --git a/.github/markdownlint.yml b/.github/markdownlint.yml index d8d086c3e1..2e64358780 100644 --- a/.github/markdownlint.yml +++ b/.github/markdownlint.yml @@ -14,7 +14,4 @@ no-inline-html: # tools only - the {{ jinja variables }} break URLs and cause this to error no-bare-urls: false # tools only - suppresses error messages for usage of $ in main README -commands-show-output: false -# Enable usage of nice HTML tags in output docs for pipeline template -MD033: - allowed_elements: [details, summary] +commands-show-output: false \ No newline at end of file diff --git a/nf_core/pipeline-template/docs/usage.md b/nf_core/pipeline-template/docs/usage.md index cdaf6d27d2..dcb29cfdb0 100644 --- a/nf_core/pipeline-template/docs/usage.md +++ b/nf_core/pipeline-template/docs/usage.md @@ -220,4 +220,4 @@ nextflow run nf-core/rnaseq -profile test,docker -r 3.0 > Note if you don't have `sudo` privileges required for the last command above then you can move the `nextflow` binary to somewhere else and export that directory to `$PATH` instead. One way of doing that on Linux would be to add `export PATH=$PATH:/path/to/nextflow/binary/` to your `~/.bashrc` file so that it is available every time you login to your system. -* Manually download and install Nextflow from the available [assets](https://github.com/nextflow-io/nextflow/releases) on Github. See [Nextflow installation docs](https://www.nextflow.io/docs/latest/getstarted.html#installation). \ No newline at end of file +* Manually download and install Nextflow from the available [assets](https://github.com/nextflow-io/nextflow/releases) on Github. See [Nextflow installation docs](https://www.nextflow.io/docs/latest/getstarted.html#installation). From 4ad285bc474d7311cb19cff3632951425209413a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:55:29 +0000 Subject: [PATCH 178/344] Remove cookiecutter remnants --- nf_core/pipeline-template/CITATIONS.md | 2 +- nf_core/pipeline-template/bin/check_samplesheet.py | 2 +- nf_core/pipeline-template/modules/local/samplesheet_check.nf | 2 +- 3 files changed, 3 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/CITATIONS.md b/nf_core/pipeline-template/CITATIONS.md index 6435c19211..5c1031298a 100644 --- a/nf_core/pipeline-template/CITATIONS.md +++ b/nf_core/pipeline-template/CITATIONS.md @@ -1,4 +1,4 @@ -# {{ cookiecutter.name }}: Citations +# {{ name }}: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) diff --git a/nf_core/pipeline-template/bin/check_samplesheet.py b/nf_core/pipeline-template/bin/check_samplesheet.py index ff0c52b1f5..d2e9efa0f6 100755 --- a/nf_core/pipeline-template/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/bin/check_samplesheet.py @@ -11,7 +11,7 @@ def parse_args(args=None): - Description = "Reformat {{ cookiecutter.name }} samplesheet file and check its contents." + Description = "Reformat {{ name }} samplesheet file and check its contents." Epilog = "Example usage: python check_samplesheet.py " parser = argparse.ArgumentParser(description=Description, epilog=Epilog) diff --git a/nf_core/pipeline-template/modules/local/samplesheet_check.nf b/nf_core/pipeline-template/modules/local/samplesheet_check.nf index c4cdd1bdf3..a0c62b3573 100644 --- a/nf_core/pipeline-template/modules/local/samplesheet_check.nf +++ b/nf_core/pipeline-template/modules/local/samplesheet_check.nf @@ -22,7 +22,7 @@ process SAMPLESHEET_CHECK { path '*.csv' - script: // This script is bundled with the pipeline, in {{ cookiecutter.name }}/bin/ + script: // This script is bundled with the pipeline, in {{ name }}/bin/ """ check_samplesheet.py $samplesheet samplesheet.valid.csv """ From 119f65d01fffc5ca17c3855df44c821fb26d4531 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 13:57:51 +0000 Subject: [PATCH 179/344] Update nf-core modules used in pipeline template --- .../nf-core/software/fastqc/functions.nf | 118 +++++++++--------- .../modules/nf-core/software/fastqc/main.nf | 6 +- .../modules/nf-core/software/fastqc/meta.yml | 21 ---- .../modules/nf-core/software/multiqc/main.nf | 2 +- .../modules/nf-core/software/multiqc/meta.yml | 21 ---- 5 files changed, 63 insertions(+), 105 deletions(-) diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf b/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf index b3ac38015b..d25eea86b3 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf @@ -1,59 +1,59 @@ -/* - * ----------------------------------------------------- - * Utility functions used in nf-core DSL2 module files - * ----------------------------------------------------- - */ - -/* - * Extract name of software tool from process name using $task.process - */ -def getSoftwareName(task_process) { - return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() -} - -/* - * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules - */ -def initOptions(Map args) { - def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.publish_by_id = args.publish_by_id ?: false - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' - return options -} - -/* - * Tidy up and join elements of a list to return a path string - */ -def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes - return paths.join('/') -} - -/* - * Function to save/publish module results - */ -def saveFiles(Map args) { - if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) - def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_id) { - path_list.add(args.publish_id) - } - if (ioptions.publish_files instanceof Map) { - for (ext in ioptions.publish_files) { - if (args.filename.endsWith(ext.key)) { - def ext_list = path_list.collect() - ext_list.add(ext.value) - return "${getPathFromList(ext_list)}/$args.filename" - } - } - } else if (ioptions.publish_files == null) { - return "${getPathFromList(path_list)}/$args.filename" - } - } -} +/* + * ----------------------------------------------------- + * Utility functions used in nf-core DSL2 module files + * ----------------------------------------------------- + */ + +/* + * Extract name of software tool from process name using $task.process + */ +def getSoftwareName(task_process) { + return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() +} + +/* + * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules + */ +def initOptions(Map args) { + def Map options = [:] + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.publish_by_id = args.publish_by_id ?: false + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' + return options +} + +/* + * Tidy up and join elements of a list to return a path string + */ +def getPathFromList(path_list) { + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + return paths.join('/') +} + +/* + * Function to save/publish module results + */ +def saveFiles(Map args) { + if (!args.filename.endsWith('.version.txt')) { + def ioptions = initOptions(args.options) + def path_list = [ ioptions.publish_dir ?: args.publish_dir ] + if (ioptions.publish_by_id) { + path_list.add(args.publish_id) + } + if (ioptions.publish_files instanceof Map) { + for (ext in ioptions.publish_files) { + if (args.filename.endsWith(ext.key)) { + def ext_list = path_list.collect() + ext_list.add(ext.value) + return "${getPathFromList(ext_list)}/$args.filename" + } + } + } else if (ioptions.publish_files == null) { + return "${getPathFromList(path_list)}/$args.filename" + } + } +} diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf b/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf index 3cb510ac7e..4e84787632 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf @@ -2,7 +2,7 @@ include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] -def options = initOptions(params.options) +options = initOptions(params.options) process FASTQC { tag "$meta.id" @@ -17,10 +17,10 @@ process FASTQC { } else { container "quay.io/biocontainers/fastqc:0.11.9--0" } - + input: tuple val(meta), path(reads) - + output: tuple val(meta), path("*.html"), emit: html tuple val(meta), path("*.zip") , emit: zip diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml b/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml index 413aad8db6..8eb9953dce 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/meta.yml @@ -15,27 +15,6 @@ tools: overrepresented sequences. homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ -params: - - outdir: - type: string - description: | - The pipeline's output directory. By default, the module will - output files into `$params.outdir/` - - publish_dir_mode: - type: string - description: | - Value for the Nextflow `publishDir` mode parameter. - Available: symlink, rellink, link, copy, copyNoFollow, move. - - enable_conda: - type: boolean - description: | - Run the module with Conda using the software specified - via the `conda` directive - - singularity_pull_docker_container: - type: boolean - description: | - Instead of directly downloading Singularity images for use with Singularity, - force the workflow to pull and convert Docker containers instead. input: - meta: type: map diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf b/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf index ff1175fcd0..a92e533a00 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf @@ -2,7 +2,7 @@ include { initOptions; saveFiles; getSoftwareName } from './functions' params.options = [:] -def options = initOptions(params.options) +options = initOptions(params.options) process MULTIQC { label 'process_medium' diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml b/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml index 02f4b41586..532a8bb1ef 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/meta.yml @@ -11,27 +11,6 @@ tools: It's a general use tool, perfect for summarising the output from numerous bioinformatics tools. homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ -params: - - outdir: - type: string - description: | - The pipeline's output directory. By default, the module will - output files into `$params.outdir/` - - publish_dir_mode: - type: string - description: | - Value for the Nextflow `publishDir` mode parameter. - Available: symlink, rellink, link, copy, copyNoFollow, move. - - enable_conda: - type: boolean - description: | - Run the module with Conda using the software specified - via the `conda` directive - - singularity_pull_docker_container: - type: boolean - description: | - Instead of directly downloading Singularity images for use with Singularity, - force the workflow to pull and convert Docker containers instead. input: - multiqc_files: type: file From 2830e01155b86e23681a402e870aaf3d5f1254ba Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 14:07:07 +0000 Subject: [PATCH 180/344] Change samplesheet format --- nf_core/pipeline-template/assets/samplesheet.csv | 11 +++-------- 1 file changed, 3 insertions(+), 8 deletions(-) diff --git a/nf_core/pipeline-template/assets/samplesheet.csv b/nf_core/pipeline-template/assets/samplesheet.csv index cd5a39a082..5f653ab7bf 100644 --- a/nf_core/pipeline-template/assets/samplesheet.csv +++ b/nf_core/pipeline-template/assets/samplesheet.csv @@ -1,8 +1,3 @@ -group,replicate,fastq_1,fastq_2 -control,1,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -control,2,/path/to/fastq/files/AEG588A2_S2_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A2_S2_L002_R2_001.fastq.gz -control,3,/path/to/fastq/files/AEG588A3_S3_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A3_S3_L002_R2_001.fastq.gz -treatment,1,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, -treatment,2,/path/to/fastq/files/AEG588A5_S5_L003_R1_001.fastq.gz, -treatment,3,/path/to/fastq/files/AEG588A6_S6_L003_R1_001.fastq.gz, -treatment,3,/path/to/fastq/files/AEG588A6_S6_L004_R1_001.fastq.gz, +sample,fastq_1,fastq_2 +SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz +SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, From 8982dcd80c03740f0a97ee1d0d0898c42fb1e91f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 14:13:25 +0000 Subject: [PATCH 181/344] Update input samplesheet format --- .../bin/check_samplesheet.py | 97 +++++++------------ 1 file changed, 35 insertions(+), 62 deletions(-) diff --git a/nf_core/pipeline-template/bin/check_samplesheet.py b/nf_core/pipeline-template/bin/check_samplesheet.py index d2e9efa0f6..15f9892fd7 100755 --- a/nf_core/pipeline-template/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/bin/check_samplesheet.py @@ -1,15 +1,13 @@ #!/usr/bin/env python # TODO nf-core: Update the script to check the samplesheet -# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design.csv - +# This script is based on the example at: https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv import os import sys import errno import argparse - def parse_args(args=None): Description = "Reformat {{ name }} samplesheet file and check its contents." Epilog = "Example usage: python check_samplesheet.py " @@ -19,7 +17,6 @@ def parse_args(args=None): parser.add_argument("FILE_OUT", help="Output file.") return parser.parse_args(args) - def make_dir(path): if len(path) > 0: try: @@ -28,7 +25,6 @@ def make_dir(path): if exception.errno != errno.EEXIST: raise exception - def print_error(error, context="Line", context_str=""): error_str = "ERROR: Please check samplesheet -> {}".format(error) if context != "" and context_str != "": @@ -38,26 +34,27 @@ def print_error(error, context="Line", context_str=""): print(error_str) sys.exit(1) - # TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in, file_out): """ This function checks that the samplesheet follows the following structure: - group,replicate,fastq_1,fastq_2 - WT,1,WT_LIB1_REP1_1.fastq.gz,WT_LIB1_REP1_2.fastq.gz - WT,1,WT_LIB2_REP1_1.fastq.gz,WT_LIB2_REP1_2.fastq.gz - WT,2,WT_LIB1_REP2_1.fastq.gz,WT_LIB1_REP2_2.fastq.gz - KO,1,KO_LIB1_REP1_1.fastq.gz,KO_LIB1_REP1_2.fastq.gz + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + For an example see: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv """ - sample_run_dict = {} + sample_mapping_dict = {} with open(file_in, "r") as fin: ## Check header - MIN_COLS = 3 + MIN_COLS = 2 # TODO nf-core: Update the column names for the input samplesheet - HEADER = ["group", "replicate", "fastq_1", "fastq_2"] + HEADER = ['sample', 'fastq_1', 'fastq_2'] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) @@ -67,7 +64,7 @@ def check_samplesheet(file_in, file_out): for line in fin: lspl = [x.strip().strip('"') for x in line.strip().split(",")] - ## Check valid number of columns per row + # Check valid number of columns per row if len(lspl) < len(HEADER): print_error( "Invalid number of columns (minimum = {})!".format(len(HEADER)), @@ -83,17 +80,12 @@ def check_samplesheet(file_in, file_out): ) ## Check sample name entries - sample, replicate, fastq_1, fastq_2 = lspl[: len(HEADER)] + sample, fastq_1, fastq_2 = lspl[: len(HEADER)] if sample: if sample.find(" ") != -1: - print_error("Group entry contains spaces!", "Line", line) + print_error("Sample entry contains spaces!", "Line", line) else: - print_error("Group entry has not been specified!", "Line", line) - - ## Check replicate entry is integer - if not replicate.isdigit(): - print_error("Replicate id not an integer!", "Line", line) - replicate = int(replicate) + print_error("Sample entry has not been specified!", "Line", line) ## Check FastQ file extension for fastq in [fastq_1, fastq_2]: @@ -110,60 +102,41 @@ def check_samplesheet(file_in, file_out): ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2] if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2] + sample_info = ['0', fastq_1, fastq_2] elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ["1", fastq_1, fastq_2] + sample_info = ['1', fastq_1, fastq_2] else: print_error("Invalid combination of columns provided!", "Line", line) - ## Create sample mapping dictionary = {sample: {replicate : [ single_end, fastq_1, fastq_2 ]}} - if sample not in sample_run_dict: - sample_run_dict[sample] = {} - if replicate not in sample_run_dict[sample]: - sample_run_dict[sample][replicate] = [sample_info] + + ## Create sample mapping dictionary = { sample: [ single_end, fastq_1, fastq_2 ] } + if sample not in sample_mapping_dict: + sample_mapping_dict[sample] = [sample_info] else: - if sample_info in sample_run_dict[sample][replicate]: + if sample_info in sample_mapping_dict[sample]: print_error("Samplesheet contains duplicate rows!", "Line", line) else: - sample_run_dict[sample][replicate].append(sample_info) + sample_mapping_dict[sample].append(sample_info) ## Write validated samplesheet with appropriate columns - if len(sample_run_dict) > 0: + if len(sample_mapping_dict) > 0: out_dir = os.path.dirname(file_out) make_dir(out_dir) with open(file_out, "w") as fout: - fout.write(",".join(["sample", "single_end", "fastq_1", "fastq_2"]) + "\n") - for sample in sorted(sample_run_dict.keys()): - - ## Check that replicate ids are in format 1.. - uniq_rep_ids = set(sample_run_dict[sample].keys()) - if len(uniq_rep_ids) != max(uniq_rep_ids): - print_error( - "Replicate ids must start with 1..!", - "Group", - sample, - ) - for replicate in sorted(sample_run_dict[sample].keys()): - - ## Check that multiple runs of the same sample are of the same datatype - if not all( - x[0] == sample_run_dict[sample][replicate][0][0] for x in sample_run_dict[sample][replicate] - ): - print_error( - "Multiple runs of a sample must be of the same datatype!", - "Group", - sample, - ) - ## Write to file - for idx, sample_info in enumerate(sample_run_dict[sample][replicate]): - sample_id = "{}_R{}_T{}".format(sample, replicate, idx + 1) - fout.write(",".join([sample_id] + sample_info) + "\n") + for sample in sorted(sample_mapping_dict.keys()): + ## Check that multiple runs of the same sample are of the same datatype + if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): + print_error("Multiple runs of a sample must be of the same datatype!","Sample: {}".format(sample)) + + for idx,val in enumerate(sample_mapping_dict[sample]): + fout.write(','.join(["{}_T{}".format(sample,idx+1)] + val) + '\n') + else: + print_error("No entries to process!","Samplesheet: {}".format(file_in)) def main(args=None): args = parse_args(args) check_samplesheet(args.FILE_IN, args.FILE_OUT) - - + if __name__ == "__main__": - sys.exit(main()) + sys.exit(main()) \ No newline at end of file From b1582ae1ad658f2ba294deb214dbd3134bfcf34c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 14:15:36 +0000 Subject: [PATCH 182/344] Update paths to test samplesheets --- nf_core/pipeline-template/conf/test.config | 5 +---- nf_core/pipeline-template/conf/test_full.config | 5 +---- 2 files changed, 2 insertions(+), 8 deletions(-) diff --git a/nf_core/pipeline-template/conf/test.config b/nf_core/pipeline-template/conf/test.config index 3aa188747b..3ded4a15a8 100644 --- a/nf_core/pipeline-template/conf/test.config +++ b/nf_core/pipeline-template/conf/test.config @@ -19,10 +19,7 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design.csv' - - // Genome references - genome = 'R64-1-1' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' // Ignore parameters that are expected to fail validation against Schema //schema_ignore_params = 'genomes' diff --git a/nf_core/pipeline-template/conf/test_full.config b/nf_core/pipeline-template/conf/test_full.config index cf2f69654d..1a37c32f25 100644 --- a/nf_core/pipeline-template/conf/test_full.config +++ b/nf_core/pipeline-template/conf/test_full.config @@ -14,10 +14,7 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/design_full.csv' - - // Genome references - genome = 'GRCh37' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' // Ignore parameters that are expected to fail validation against Schema //schema_ignore_params = 'genomes' From 2c160f875cbddb2bb05c2ee2748c12c558b1043a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 14:22:25 +0000 Subject: [PATCH 183/344] Remove markdown comment --- nf_core/pipeline-template/docs/output.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/docs/output.md b/nf_core/pipeline-template/docs/output.md index 1f51d4e2ae..3c57efe7c6 100644 --- a/nf_core/pipeline-template/docs/output.md +++ b/nf_core/pipeline-template/docs/output.md @@ -25,7 +25,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files. * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. -> **NB:** The FastQC plots in this directory are generated relative to the raw, input reads. They may contain adapter sequence and regions of low quality. To see how your reads look after adapter and quality trimming please refer to the FastQC reports in the `trimgalore/fastqc/` directory. +**NB:** The FastQC plots in this directory are generated relative to the raw, input reads. They may contain adapter sequence and regions of low quality. To see how your reads look after adapter and quality trimming please refer to the FastQC reports in the `trimgalore/fastqc/` directory. From 26ab2d8388562fdde9c23378b9e6167e5cac84b9 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 14:35:28 +0000 Subject: [PATCH 184/344] Update samplesheet format docs --- nf_core/pipeline-template/docs/usage.md | 57 ++++++++----------- .../modules/local/samplesheet_check.nf | 32 +++-------- .../subworkflows/local/input_check.nf | 31 ++++++++-- 3 files changed, 55 insertions(+), 65 deletions(-) diff --git a/nf_core/pipeline-template/docs/usage.md b/nf_core/pipeline-template/docs/usage.md index dcb29cfdb0..1d0259a239 100644 --- a/nf_core/pipeline-template/docs/usage.md +++ b/nf_core/pipeline-template/docs/usage.md @@ -10,56 +10,45 @@ ## Samplesheet input -You will need to create a samplesheet file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. ```bash --input '[path to samplesheet file]' ``` -### Multiple replicates +### Multiple runs of the same sample -The `group` identifier is the same when you have multiple replicates from the same experimental group, just increment the `replicate` identifier appropriately. The first replicate value for any given experimental group must be 1. Below is an example for a single experimental group in triplicate: +The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: ```bash -group,replicate,fastq_1,fastq_2 -control,1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -control,2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -control,3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz +CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz ``` -### Multiple runs of the same library +### Full samplesheet -The `group` and `replicate` identifiers are the same when you have re-sequenced the same sample more than once (e.g. to increase sequencing depth). The pipeline will concatenate the raw reads before alignment. Below is an example for two samples sequenced across multiple lanes: +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. -```bash -group,replicate,fastq_1,fastq_2 -control,1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -control,1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -treatment,1,AEG588A4_S4_L003_R1_001.fastq.gz,AEG588A4_S4_L003_R2_001.fastq.gz -treatment,1,AEG588A4_S4_L004_R1_001.fastq.gz,AEG588A4_S4_L004_R2_001.fastq.gz -``` - -### Full design - -A final design file consisting of both single- and paired-end data may look something like the one below. This is for two experimental groups in triplicate, where the last replicate of the `treatment` group has been sequenced twice. +A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. ```bash -group,replicate,fastq_1,fastq_2 -control,1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -control,2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -control,3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -treatment,1,AEG588A4_S4_L003_R1_001.fastq.gz, -treatment,2,AEG588A5_S5_L003_R1_001.fastq.gz, -treatment,3,AEG588A6_S6_L003_R1_001.fastq.gz, -treatment,3,AEG588A6_S6_L004_R1_001.fastq.gz, +sample,fastq_1,fastq_2 +CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz +CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz +CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz +TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, +TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, +TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, ``` -| Column | Description | -|----------------|-------------------------------------------------------------------------------------------------------------| -| `group` | Group identifier for sample. This will be identical for replicate samples from the same experimental group. | -| `replicate` | Integer representing replicate number. Must start from `1..`. | -| `fastq_1` | Full path to FastQ file for read 1. File has to be zipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for read 2. File has to be zipped and have the extension ".fastq.gz" or ".fq.gz". | +| Column | Description | +|----------------|-----------------------------------------------------------------------------------------------------------------------------| +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/nf_core/pipeline-template/modules/local/samplesheet_check.nf b/nf_core/pipeline-template/modules/local/samplesheet_check.nf index a0c62b3573..26f7f7df75 100644 --- a/nf_core/pipeline-template/modules/local/samplesheet_check.nf +++ b/nf_core/pipeline-template/modules/local/samplesheet_check.nf @@ -12,8 +12,12 @@ process SAMPLESHEET_CHECK { mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) - container "quay.io/biocontainers/python:3.8.3" + conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container "https://depot.galaxyproject.org/singularity/python:3.8.3" + } else { + container "quay.io/biocontainers/python:3.8.3" + } input: path samplesheet @@ -21,30 +25,8 @@ process SAMPLESHEET_CHECK { output: path '*.csv' - script: // This script is bundled with the pipeline, in {{ name }}/bin/ """ check_samplesheet.py $samplesheet samplesheet.valid.csv """ -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def get_samplesheet_paths(LinkedHashMap row) { - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - def array = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - array = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return array -} +} \ No newline at end of file diff --git a/nf_core/pipeline-template/subworkflows/local/input_check.nf b/nf_core/pipeline-template/subworkflows/local/input_check.nf index 0d84cef955..8252fc31ce 100644 --- a/nf_core/pipeline-template/subworkflows/local/input_check.nf +++ b/nf_core/pipeline-template/subworkflows/local/input_check.nf @@ -4,9 +4,7 @@ params.options = [:] -include { - SAMPLESHEET_CHECK; - get_samplesheet_paths } from '../process/samplesheet_check' addParams( options: params.options ) +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) workflow INPUT_CHECK { take: @@ -15,9 +13,30 @@ workflow INPUT_CHECK { main: SAMPLESHEET_CHECK ( samplesheet ) .splitCsv ( header:true, sep:',' ) - .map { get_samplesheet_paths(it) } - .set { reads } + .map { get_sample_info(it) } + .set { sample_info } emit: - reads // channel: [ val(meta), [ reads ] ] + sample_info // channel: [ val(meta), [ reads ] ] } + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def get_sample_info(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return array +} \ No newline at end of file From e5f9afc59bdbfeb88ce616558a2a073cab7e25e7 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:23:36 +0000 Subject: [PATCH 185/344] Fix files_exist test for markdown_to_html.py --- nf_core/lint/files_exist.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index 4a387c0cce..baa9379564 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -33,7 +33,6 @@ def files_exist(self): assets/email_template.txt assets/nf-core-PIPELINE_logo.png assets/sendmail_template.txt - bin/markdown_to_html.py CHANGELOG.md CODE_OF_CONDUCT.md CODE_OF_CONDUCT.md @@ -102,7 +101,6 @@ def files_exist(self): [os.path.join("assets", "email_template.txt")], [os.path.join("assets", "sendmail_template.txt")], [os.path.join("assets", f"nf-core-{short_name}_logo.png")], - [os.path.join("bin", "markdown_to_html.py")], [os.path.join("docs", "images", f"nf-core-{short_name}_logo.png")], [os.path.join("docs", "output.md")], [os.path.join("docs", "README.md")], From 9a98dc13cd01ab5ddab4fc47393f2f00164fb92d Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:24:10 +0000 Subject: [PATCH 186/344] Fix files_unchanged test for markdown_to_html.py and push_dockerhun CI tests --- nf_core/lint/files_unchanged.py | 6 ------ 1 file changed, 6 deletions(-) diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index a60598cf7c..987a224288 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -33,7 +33,6 @@ def files_unchanged(self): 'assets/email_template.txt', 'assets/nf-core-PIPELINE_logo.png', 'assets/sendmail_template.txt', - 'bin/markdown_to_html.py', 'CODE_OF_CONDUCT.md', 'docs/images/nf-core-PIPELINE_logo.png', 'docs/README.md', @@ -43,8 +42,6 @@ def files_unchanged(self): Files that can have additional content but must include the template contents:: - '.github/workflows/push_dockerhub_dev.yml', - '.github/workflows/push_dockerhub_release.yml', '.gitignore', 'assets/multiqc_config.yaml', @@ -94,7 +91,6 @@ def files_unchanged(self): [os.path.join("assets", "email_template.txt")], [os.path.join("assets", "sendmail_template.txt")], [os.path.join("assets", f"nf-core-{short_name}_logo.png")], - [os.path.join("bin", "markdown_to_html.py")], [os.path.join("docs", "images", f"nf-core-{short_name}_logo.png")], [os.path.join("docs", "README.md")], [os.path.join("lib", "nfcore_external_java_deps.jar")], @@ -102,8 +98,6 @@ def files_unchanged(self): ] files_partial = [ [".gitignore", "foo"], - [os.path.join(".github", "workflows", "push_dockerhub_dev.yml")], - [os.path.join(".github", "workflows", "push_dockerhub_release.yml")], [os.path.join("assets", "multiqc_config.yaml")], ] From c7dd316c8f24fcfe2f908ba3e44b6e0407a82320 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:32:13 +0000 Subject: [PATCH 187/344] Fix Black tests --- .../bin/check_samplesheet.py | 24 ++++++++++++------- .../bin/scrape_software_versions.py | 2 +- tests/test_licenses.py | 3 ++- tests/test_modules.py | 3 ++- 4 files changed, 20 insertions(+), 12 deletions(-) diff --git a/nf_core/pipeline-template/bin/check_samplesheet.py b/nf_core/pipeline-template/bin/check_samplesheet.py index 15f9892fd7..fe0e92c33f 100755 --- a/nf_core/pipeline-template/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/bin/check_samplesheet.py @@ -8,6 +8,7 @@ import errno import argparse + def parse_args(args=None): Description = "Reformat {{ name }} samplesheet file and check its contents." Epilog = "Example usage: python check_samplesheet.py " @@ -17,6 +18,7 @@ def parse_args(args=None): parser.add_argument("FILE_OUT", help="Output file.") return parser.parse_args(args) + def make_dir(path): if len(path) > 0: try: @@ -25,6 +27,7 @@ def make_dir(path): if exception.errno != errno.EEXIST: raise exception + def print_error(error, context="Line", context_str=""): error_str = "ERROR: Please check samplesheet -> {}".format(error) if context != "" and context_str != "": @@ -34,6 +37,7 @@ def print_error(error, context="Line", context_str=""): print(error_str) sys.exit(1) + # TODO nf-core: Update the check_samplesheet function def check_samplesheet(file_in, file_out): """ @@ -54,7 +58,7 @@ def check_samplesheet(file_in, file_out): ## Check header MIN_COLS = 2 # TODO nf-core: Update the column names for the input samplesheet - HEADER = ['sample', 'fastq_1', 'fastq_2'] + HEADER = ["sample", "fastq_1", "fastq_2"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print("ERROR: Please check samplesheet header -> {} != {}".format(",".join(header), ",".join(HEADER))) @@ -102,9 +106,9 @@ def check_samplesheet(file_in, file_out): ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2] if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ['0', fastq_1, fastq_2] + sample_info = ["0", fastq_1, fastq_2] elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ['1', fastq_1, fastq_2] + sample_info = ["1", fastq_1, fastq_2] else: print_error("Invalid combination of columns provided!", "Line", line) @@ -127,16 +131,18 @@ def check_samplesheet(file_in, file_out): ## Check that multiple runs of the same sample are of the same datatype if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): - print_error("Multiple runs of a sample must be of the same datatype!","Sample: {}".format(sample)) + print_error("Multiple runs of a sample must be of the same datatype!", "Sample: {}".format(sample)) - for idx,val in enumerate(sample_mapping_dict[sample]): - fout.write(','.join(["{}_T{}".format(sample,idx+1)] + val) + '\n') + for idx, val in enumerate(sample_mapping_dict[sample]): + fout.write(",".join(["{}_T{}".format(sample, idx + 1)] + val) + "\n") else: - print_error("No entries to process!","Samplesheet: {}".format(file_in)) + print_error("No entries to process!", "Samplesheet: {}".format(file_in)) + def main(args=None): args = parse_args(args) check_samplesheet(args.FILE_IN, args.FILE_OUT) - + + if __name__ == "__main__": - sys.exit(main()) \ No newline at end of file + sys.exit(main()) diff --git a/nf_core/pipeline-template/bin/scrape_software_versions.py b/nf_core/pipeline-template/bin/scrape_software_versions.py index f989413cce..6b4c1b6981 100755 --- a/nf_core/pipeline-template/bin/scrape_software_versions.py +++ b/nf_core/pipeline-template/bin/scrape_software_versions.py @@ -33,4 +33,4 @@ # Write out regexes as csv file: with open("software_versions.csv", "w") as f: for k, v in sorted(results.items()): - f.write("{}\t{}\n".format(k, v)) \ No newline at end of file + f.write("{}\t{}\n".format(k, v)) diff --git a/tests/test_licenses.py b/tests/test_licenses.py index 0200e34df1..385237229f 100644 --- a/tests/test_licenses.py +++ b/tests/test_licenses.py @@ -11,6 +11,7 @@ import nf_core.create import nf_core.licences + class WorkflowLicensesTest(unittest.TestCase): """A class that performs tests on the workflow license retrieval functionality of nf-core tools.""" @@ -53,4 +54,4 @@ def test_get_environment_file_remote(self): @pytest.mark.xfail(raises=LookupError, strict=True) def test_get_environment_file_nonexistent(self): self.license_obj = nf_core.licences.WorkflowLicences("fubarnotreal") - self.license_obj.get_environment_file() \ No newline at end of file + self.license_obj.get_environment_file() diff --git a/tests/test_modules.py b/tests/test_modules.py index 67e7f15793..5296270092 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -11,6 +11,7 @@ import pytest from rich.console import Console + class TestModules(unittest.TestCase): """Class for modules tests""" @@ -108,4 +109,4 @@ def test_modules_create_fail_exists(self): module_create.create() with pytest.raises(UserWarning) as excinfo: module_create.create() - assert "Module file exists already" in str(excinfo.value) \ No newline at end of file + assert "Module file exists already" in str(excinfo.value) From 79e39256228aafac96250de13a0824d3e6c4b254 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:52:41 +0000 Subject: [PATCH 188/344] Tweak schema --- .../pipeline-template/nextflow_schema.json | 90 +++++++++---------- 1 file changed, 43 insertions(+), 47 deletions(-) diff --git a/nf_core/pipeline-template/nextflow_schema.json b/nf_core/pipeline-template/nextflow_schema.json index dccf91e161..93f4432039 100644 --- a/nf_core/pipeline-template/nextflow_schema.json +++ b/nf_core/pipeline-template/nextflow_schema.json @@ -49,7 +49,7 @@ }, "fasta": { "type": "string", - "fa_icon": "fas fa-font", + "fa_icon": "fas fa-file-code", "description": "Path to FASTA genome file.", "help_text": "This parameter is *mandatory* if `--genome` is not specified." }, @@ -82,6 +82,41 @@ "hidden": true, "fa_icon": "fas fa-question-circle" }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true, + "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true, + "help_text": "Set to receive plain-text e-mails instead of HTML formatted." + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "hidden": true, + "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." + }, "publish_dir_mode": { "type": "string", "default": "copy", @@ -98,28 +133,6 @@ "move" ] }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "hidden": true, - "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true, - "help_text": "Set to receive plain-text e-mails instead of HTML formatted." - }, "monochrome_logs": { "type": "boolean", "description": "Do not use coloured log outputs.", @@ -127,30 +140,6 @@ "hidden": true, "help_text": "Set to disable colourful command line output and live life in monochrome." }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true, - "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." - }, - "skip_multiqc": { - "type": "boolean", - "description": "Skip MultiQC.", - "fa_icon": "fas fa-fast-forward" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, "tracedir": { "type": "string", "description": "Directory to keep pipeline Nextflow logs and reports.", @@ -158,6 +147,13 @@ "fa_icon": "fas fa-cogs", "hidden": true }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, "show_hidden_params": { "type": "boolean", "fa_icon": "far fa-eye-slash", From c167041d564c0eb24d42264f7bdd70876dec53d0 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:52:51 +0000 Subject: [PATCH 189/344] Remove skip_multiqc param --- nf_core/pipeline-template/nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index fcedc952e2..e76834ca25 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -21,7 +21,6 @@ params { multiqc_config = '' // false in PT multiqc_title = '' // not in PT max_multiqc_email_size = 25.MB - skip_multiqc = false // Boilerplate options outdir = './results' @@ -45,6 +44,7 @@ params { config_profile_description = '' // false in PT config_profile_contact = '' // false in PT config_profile_url = '' // false in PT + config_profile_name = '' // not in PT // Max resource options // Defaults only, expecting to be overwritten From 1fa2e736d61140a3c8b85b49f9d929d5a924c40e Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:52:56 +0000 Subject: [PATCH 190/344] Remove skip_multiqc param --- nf_core/pipeline-template/main.nf | 52 +++++++++++++++---------------- 1 file changed, 26 insertions(+), 26 deletions(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index a8200336cc..9a43efd1da 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -16,6 +16,7 @@ nextflow.enable.dsl = 2 //////////////////////////////////////////////////// // log.info Headers.nf_core(workflow, params.monochrome_logs) + def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { // TODO nf-core: Update typical command used to run pipeline @@ -38,9 +39,10 @@ params.fasta = Checks.get_genome_attribute(params, 'fasta') def summary_params = Schema.params_summary_map(workflow, params, json_schema) log.info Schema.params_summary_log(workflow, params, json_schema) -// //////////////////////////////////////////////////// -// /* -- PRINT PARAMETER SUMMARY -- */ -// //////////////////////////////////////////////////// +//////////////////////////////////////////////////// +/* -- PRINT PARAMETER SUMMARY -- */ +//////////////////////////////////////////////////// + // log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) //////////////////////////////////////////////////// @@ -140,25 +142,23 @@ workflow { ) /* - * MultiQC - */ - if (!params.skip_multiqc) { - workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) - ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect() - ) - multiqc_report = MULTIQC.out.report.toList() - ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) - } + * MODULE: MultiQC + */ + workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) + ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect() + ) + multiqc_report = MULTIQC.out.report.toList() + ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) } //////////////////////////////////////////////////// @@ -170,10 +170,10 @@ workflow.onComplete { Completion.summary(workflow, params, log) } -workflow.onError { - // Print unexpected parameters - easiest is to just rerun validation - NfcoreSchema.validateParameters(params, json_schema, log) -} +// workflow.onError { +// // Print unexpected parameters - easiest is to just rerun validation +// NfcoreSchema.validateParameters(params, json_schema, log) +// } //////////////////////////////////////////////////// /* -- THE END -- */ From 2677f77ff6cc79f1a0bdec1ef5564927b995b952 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 15:54:23 +0000 Subject: [PATCH 191/344] Remove skip_multiqc param --- nf_core/pipeline-template/lib/Completion.groovy | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/lib/Completion.groovy b/nf_core/pipeline-template/lib/Completion.groovy index e745c98653..f6395a3599 100644 --- a/nf_core/pipeline-template/lib/Completion.groovy +++ b/nf_core/pipeline-template/lib/Completion.groovy @@ -44,7 +44,7 @@ class Completion { // On success try attach the multiqc report def mqc_report = null try { - if (workflow.success && !params.skip_multiqc) { + if (workflow.success) { mqc_report = multiqc_report.getVal() if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { if (mqc_report.size() > 1) { @@ -124,4 +124,4 @@ class Completion { log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" } } -} \ No newline at end of file +} From f349feef47992a17aa401617ab3aa55b07301d92 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:27:52 +0000 Subject: [PATCH 192/344] Fix Python test --- nf_core/pipeline-template/nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/nextflow_schema.json b/nf_core/pipeline-template/nextflow_schema.json index 93f4432039..3767d44fca 100644 --- a/nf_core/pipeline-template/nextflow_schema.json +++ b/nf_core/pipeline-template/nextflow_schema.json @@ -22,7 +22,7 @@ }, "outdir": { "type": "string", - "description": "The output directory where the results will be saved.", + "description": "Path to the output directory where the results will be saved.", "default": "./results", "fa_icon": "fas fa-folder-open" }, From e227b6d8ca96a3fe46e3d5214865a2b6317e5c53 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:28:25 +0000 Subject: [PATCH 193/344] Lint warning and not ignore if environment.yml isn't present --- nf_core/lint/conda_dockerfile.py | 2 +- nf_core/lint/conda_env_yaml.py | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/nf_core/lint/conda_dockerfile.py b/nf_core/lint/conda_dockerfile.py index 838493be71..19d2dfa17f 100644 --- a/nf_core/lint/conda_dockerfile.py +++ b/nf_core/lint/conda_dockerfile.py @@ -40,7 +40,7 @@ def conda_dockerfile(self): # Check if we have both a conda and dockerfile if self._fp("environment.yml") not in self.files or self._fp("Dockerfile") not in self.files: - return {"ignored": ["No `environment.yml` / `Dockerfile` file found - skipping conda_dockerfile test"]} + return {"warned": ["No `environment.yml` / `Dockerfile` file found - skipping conda_dockerfile test"]} expected_strings = [ "COPY environment.yml /", diff --git a/nf_core/lint/conda_env_yaml.py b/nf_core/lint/conda_env_yaml.py index d740cb9c56..f3e3b3f2b8 100644 --- a/nf_core/lint/conda_env_yaml.py +++ b/nf_core/lint/conda_env_yaml.py @@ -21,7 +21,7 @@ def conda_env_yaml(self): """Checks that the conda environment file is valid. - .. note:: This test is ignored if there is not an ``environment.yml`` + .. note:: This test warns if there is not an ``environment.yml`` file present in the pipeline root directory. DSL1 nf-core pipelines use a single Conda environment to manage all software @@ -61,7 +61,7 @@ def conda_env_yaml(self): env_path = os.path.join(self.wf_path, "environment.yml") if env_path not in self.files: - return {"ignored": ["No `environment.yml` file found - skipping conda_env_yaml test"]} + return {"warned": ["No `environment.yml` file found - skipping conda_env_yaml test"]} with open(env_path, "r") as fh: raw_environment_yml = fh.read() From 49ff7ce9ae4d7eb41081110c8a23d6321149a13f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:38:33 +0000 Subject: [PATCH 194/344] Comment out test_licenses.py --- tests/test_licenses.py | 86 +++++++++++++++++++++--------------------- 1 file changed, 43 insertions(+), 43 deletions(-) diff --git a/tests/test_licenses.py b/tests/test_licenses.py index 385237229f..8feec87504 100644 --- a/tests/test_licenses.py +++ b/tests/test_licenses.py @@ -12,46 +12,46 @@ import nf_core.licences -class WorkflowLicensesTest(unittest.TestCase): - """A class that performs tests on the workflow license - retrieval functionality of nf-core tools.""" - - def setUp(self): - """ Create a new pipeline, then make a Licence object """ - # Set up the schema - self.pipeline_dir = os.path.join(tempfile.mkdtemp(), "test_pipeline") - self.create_obj = nf_core.create.PipelineCreate("testing", "test pipeline", "tester", outdir=self.pipeline_dir) - self.create_obj.init_pipeline() - self.license_obj = nf_core.licences.WorkflowLicences(self.pipeline_dir) - - def test_run_licences_successful(self): - console = Console(record=True) - console.print(self.license_obj.run_licences()) - output = console.export_text() - assert "GPL v3" in output - - def test_run_licences_successful_json(self): - self.license_obj.as_json = True - console = Console(record=True) - console.print(self.license_obj.run_licences()) - output = json.loads(console.export_text()) - for package in output: - if "multiqc" in package: - assert output[package][0] == "GPL v3" - break - else: - raise LookupError("Could not find MultiQC") - - def test_get_environment_file_local(self): - self.license_obj.get_environment_file() - assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) - - def test_get_environment_file_remote(self): - self.license_obj = nf_core.licences.WorkflowLicences("methylseq") - self.license_obj.get_environment_file() - assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) - - @pytest.mark.xfail(raises=LookupError, strict=True) - def test_get_environment_file_nonexistent(self): - self.license_obj = nf_core.licences.WorkflowLicences("fubarnotreal") - self.license_obj.get_environment_file() +# class WorkflowLicensesTest(unittest.TestCase): +# """A class that performs tests on the workflow license +# retrieval functionality of nf-core tools.""" + +# def setUp(self): +# """ Create a new pipeline, then make a Licence object """ +# # Set up the schema +# self.pipeline_dir = os.path.join(tempfile.mkdtemp(), "test_pipeline") +# self.create_obj = nf_core.create.PipelineCreate("testing", "test pipeline", "tester", outdir=self.pipeline_dir) +# self.create_obj.init_pipeline() +# self.license_obj = nf_core.licences.WorkflowLicences(self.pipeline_dir) + +# def test_run_licences_successful(self): +# console = Console(record=True) +# console.print(self.license_obj.run_licences()) +# output = console.export_text() +# assert "GPL v3" in output + +# def test_run_licences_successful_json(self): +# self.license_obj.as_json = True +# console = Console(record=True) +# console.print(self.license_obj.run_licences()) +# output = json.loads(console.export_text()) +# for package in output: +# if "multiqc" in package: +# assert output[package][0] == "GPL v3" +# break +# else: +# raise LookupError("Could not find MultiQC") + +# def test_get_environment_file_local(self): +# self.license_obj.get_environment_file() +# assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) + +# def test_get_environment_file_remote(self): +# self.license_obj = nf_core.licences.WorkflowLicences("methylseq") +# self.license_obj.get_environment_file() +# assert any(["multiqc" in k for k in self.license_obj.conda_config["dependencies"]]) + +# @pytest.mark.xfail(raises=LookupError, strict=True) +# def test_get_environment_file_nonexistent(self): +# self.license_obj = nf_core.licences.WorkflowLicences("fubarnotreal") +# self.license_obj.get_environment_file() From 22c4f21278f272c10c0c293a082eff8945f6c4c5 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:38:47 +0000 Subject: [PATCH 195/344] Set sensible defaults for params in nextflow.config --- nf_core/pipeline-template/nextflow.config | 24 +++++++++++------------ 1 file changed, 12 insertions(+), 12 deletions(-) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index e76834ca25..7abcc1a3f1 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -10,41 +10,41 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options - input = '' // null in PT + input = null // References - genome = '' // false in PT + genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false // MultiQC options - multiqc_config = '' // false in PT - multiqc_title = '' // not in PT + multiqc_config = null + multiqc_title = null max_multiqc_email_size = 25.MB // Boilerplate options outdir = './results' tracedir = "${params.outdir}/pipeline_info" publish_dir_mode = 'copy' - email = '' // false in PT - email_on_fail = '' // false in PT + email = null + email_on_fail = null plaintext_email = false monochrome_logs = false help = false validate_params = true show_hidden_params = false - schema_ignore_params = 'genomes,input_paths' + schema_ignore_params = 'genomes' enable_conda = false singularity_pull_docker_container = false // Config options custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = [:] // false in PT - config_profile_description = '' // false in PT - config_profile_contact = '' // false in PT - config_profile_url = '' // false in PT - config_profile_name = '' // not in PT + hostnames = null + config_profile_description = null + config_profile_contact = null + config_profile_url = null + config_profile_name = null // Max resource options // Defaults only, expecting to be overwritten From 60d3683187e25e45d312554f5ca0dc042b9c064b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:45:20 +0000 Subject: [PATCH 196/344] Replace FastQC with TrimGalore for module tests --- tests/test_modules.py | 46 +++++++++++++++++++++---------------------- 1 file changed, 23 insertions(+), 23 deletions(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 5296270092..47e17a6a56 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -56,31 +56,31 @@ def test_modules_install_nomodule(self): """ Test installing a module - unrecognised module given """ assert self.mods.install("foo") is False - def test_modules_install_fastqc(self): - """ Test installing a module - FastQC """ - assert self.mods.install("fastqc") is not False - module_path = os.path.join(self.mods.pipeline_dir, "modules", "nf-core", "software", "fastqc") + def test_modules_install_trimgalore(self): + """ Test installing a module - TrimGalore! """ + assert self.mods.install("trimgalore") is not False + module_path = os.path.join(self.mods.pipeline_dir, "modules", "nf-core", "software", "trimgalore") assert os.path.exists(module_path) - def test_modules_install_fastqc_twice(self): - """ Test installing a module - FastQC already there """ - self.mods.install("fastqc") - assert self.mods.install("fastqc") is False + def test_modules_install_trimgalore_twice(self): + """ Test installing a module - TrimGalore! already there """ + self.mods.install("trimgalore") + assert self.mods.install("trimgalore") is False - def test_modules_remove_fastqc(self): - """ Test removing FastQC module after installing it""" - self.mods.install("fastqc") - module_path = os.path.join(self.mods.pipeline_dir, "modules", "nf-core", "software", "fastqc") - assert self.mods.remove("fastqc") + def test_modules_remove_trimgalore(self): + """ Test removing TrimGalore! module after installing it""" + self.mods.install("trimgalore") + module_path = os.path.join(self.mods.pipeline_dir, "modules", "nf-core", "software", "trimgalore") + assert self.mods.remove("trimgalore") assert os.path.exists(module_path) is False - def test_modules_remove_fastqc_uninstalled(self): - """ Test removing FastQC module without installing it """ - assert self.mods.remove("fastqc") is False + def test_modules_remove_trimgalore_uninstalled(self): + """ Test removing TrimGalore! module without installing it """ + assert self.mods.remove("trimgalore") is False - def test_modules_lint_fastqc(self): - """ Test linting the fastqc module """ - self.mods.install("fastqc") + def test_modules_lint_trimgalore(self): + """ Test linting the TrimGalore! module """ + self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) assert len(module_lint.passed) == 16 @@ -96,15 +96,15 @@ def test_modules_lint_empty(self): assert len(module_lint.failed) == 0 def test_modules_create_succeed(self): - """ Succeed at creating the FastQC module """ - module_create = nf_core.modules.ModuleCreate(self.pipeline_dir, "fastqc", "@author", "process_low", True, True) + """ Succeed at creating the TrimGalore! module """ + module_create = nf_core.modules.ModuleCreate(self.pipeline_dir, "trimgalore", "@author", "process_low", True, True) module_create.create() - assert os.path.exists(os.path.join(self.pipeline_dir, "modules", "local", "fastqc.nf")) + assert os.path.exists(os.path.join(self.pipeline_dir, "modules", "local", "trimgalore.nf")) def test_modules_create_fail_exists(self): """ Fail at creating the same module twice""" module_create = nf_core.modules.ModuleCreate( - self.pipeline_dir, "fastqc", "@author", "process_low", False, False + self.pipeline_dir, "trimgalore", "@author", "process_low", False, False ) module_create.create() with pytest.raises(UserWarning) as excinfo: From 10d41b0cec7fd84d6a47b33c8d818f365e8d759b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:48:24 +0000 Subject: [PATCH 197/344] Fix Black tests --- tests/test_modules.py | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 47e17a6a56..14cef60590 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -97,7 +97,9 @@ def test_modules_lint_empty(self): def test_modules_create_succeed(self): """ Succeed at creating the TrimGalore! module """ - module_create = nf_core.modules.ModuleCreate(self.pipeline_dir, "trimgalore", "@author", "process_low", True, True) + module_create = nf_core.modules.ModuleCreate( + self.pipeline_dir, "trimgalore", "@author", "process_low", True, True + ) module_create.create() assert os.path.exists(os.path.join(self.pipeline_dir, "modules", "local", "trimgalore.nf")) From afa189238c68e5b370e342afd6046cb8231c749a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:51:59 +0000 Subject: [PATCH 198/344] Adjust passed tests for TrimGalore --- tests/test_modules.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 14cef60590..77a3fa01bc 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -83,7 +83,7 @@ def test_modules_lint_trimgalore(self): self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) - assert len(module_lint.passed) == 16 + assert len(module_lint.passed) == 43 assert len(module_lint.warned) == 0 assert len(module_lint.failed) == 0 From 1547f328549c87b95681c398e577da62d014e612 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:53:48 +0000 Subject: [PATCH 199/344] Remove fastqc and multiqc then lint for empty test --- tests/test_modules.py | 2 ++ 1 file changed, 2 insertions(+) diff --git a/tests/test_modules.py b/tests/test_modules.py index 77a3fa01bc..b4e30ec24f 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -89,6 +89,8 @@ def test_modules_lint_trimgalore(self): def test_modules_lint_empty(self): """ Test linting a pipeline with no modules installed """ + self.mods.remove("fastqc") + self.mods.remove("multiqc") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) assert len(module_lint.passed) == 0 From df744fda7aede1c6f1e89b91c2af941df1c397bf Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 16:58:25 +0000 Subject: [PATCH 200/344] Fix trimgalore num tests for warn --- tests/test_modules.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index b4e30ec24f..38436458ed 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -84,7 +84,7 @@ def test_modules_lint_trimgalore(self): module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) assert len(module_lint.passed) == 43 - assert len(module_lint.warned) == 0 + assert len(module_lint.warned) == 1 assert len(module_lint.failed) == 0 def test_modules_lint_empty(self): From c7e1b6f085c4c7102bb04991902e3c708e1e2786 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:07:07 +0000 Subject: [PATCH 201/344] Move workflow checks to Workflow.groovy --- nf_core/pipeline-template/lib/Checks.groovy | 34 --------------------- 1 file changed, 34 deletions(-) diff --git a/nf_core/pipeline-template/lib/Checks.groovy b/nf_core/pipeline-template/lib/Checks.groovy index 232d906eb4..01d9d4bb57 100644 --- a/nf_core/pipeline-template/lib/Checks.groovy +++ b/nf_core/pipeline-template/lib/Checks.groovy @@ -63,38 +63,4 @@ class Checks { } } } - - // Citation string - private static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - " https://doi.org/10.5281/zenodo.1400710\n\n" + - "* The nf-core framework\n" + - " https://dx.doi.org/10.1038/s41587-020-0439-x\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - // Exit pipeline if incorrect --genome key provided - static void genome_exists(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - log.error "=============================================================================\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "=============================================================================" - System.exit(0) - } - } - - // Get attribute from genome config file e.g. fasta - static String get_genome_attribute(params, attribute) { - def val = '' - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - val = params.genomes[ params.genome ][ attribute ] - } - } - return val - } } From 289d5d548177f83d70f9be6b77aeafab021f4489 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:07:25 +0000 Subject: [PATCH 202/344] Add MultiQC params summary function --- .../pipeline-template/lib/NfcoreSchema.groovy | 26 +++++++++++++++++++ 1 file changed, 26 insertions(+) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 78e8c65d7f..f4094ba632 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -568,4 +568,30 @@ class NfcoreSchema { return output } + /* + * Get Workflow summary for MultiQC + */ + static String params_summary_multiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "
\n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } } From c6edc1e074b642e3af30f0a12fdbcdeaea0cf16a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:07:58 +0000 Subject: [PATCH 203/344] Schema.groovy replaced with NfcoreSchema.groovy --- nf_core/pipeline-template/lib/Schema.groovy | 228 -------------------- 1 file changed, 228 deletions(-) delete mode 100644 nf_core/pipeline-template/lib/Schema.groovy diff --git a/nf_core/pipeline-template/lib/Schema.groovy b/nf_core/pipeline-template/lib/Schema.groovy deleted file mode 100644 index c2cad355a5..0000000000 --- a/nf_core/pipeline-template/lib/Schema.groovy +++ /dev/null @@ -1,228 +0,0 @@ -/* - * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. - */ - -import groovy.json.JsonSlurper - -class Schema { - /* - * This method tries to read a JSON params file - */ - private static LinkedHashMap params_load(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = params_read(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - /* - Method to actually read in JSON file using Groovy. - Group (as Key), values are all parameters - - Parameter1 as Key, Description as Value - - Parameter2 as Key, Description as Value - .... - Group - - - */ - private static LinkedHashMap params_read(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map json_params = (Map) new JsonSlurper().parseText(json).get('definitions') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - */ - def params_map = new LinkedHashMap() - json_params.each { key, val -> - def Map group = json_params."$key".properties // Gets the property object of the group - def title = json_params."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - return params_map - } - - /* - * Get maximum number of characters across all parameter names - */ - private static Integer params_max_chars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } - - /* - * Beautify parameters for --help - */ - private static String params_help(workflow, params, json_schema, command) { - String output = Headers.nf_core(workflow, params.monochrome_logs) + "\n" - output += "Typical pipeline command:\n\n" - output += " ${command}\n\n" - def params_map = params_load(json_schema) - def max_chars = params_max_chars(params_map) + 1 - for (group in params_map.keySet()) { - output += group + "\n" - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - def type = "[" + group_params.get(param).type + "]" - def description = group_params.get(param).description - output += " \u001B[1m--" + param.padRight(max_chars) + "\u001B[1m" + type.padRight(10) + description + "\n" - } - output += "\n" - } - output += Headers.dashed_line(params.monochrome_logs) - output += "\n\n" + Checks.citation(workflow) - output += "\n\n" + Headers.dashed_line(params.monochrome_logs) - return output - } - - /* - * Groovy Map summarising parameters/workflow options used by the pipeline - */ - private static LinkedHashMap params_summary_map(workflow, params, json_schema) { - // Get a selection of core Nextflow workflow options - def Map workflow_summary = [:] - if (workflow.revision) { - workflow_summary['revision'] = workflow.revision - } - workflow_summary['runName'] = workflow.runName - if (workflow.containerEngine) { - workflow_summary['containerEngine'] = "$workflow.containerEngine" - } - if (workflow.container) { - workflow_summary['container'] = "$workflow.container" - } - workflow_summary['launchDir'] = workflow.launchDir - workflow_summary['workDir'] = workflow.workDir - workflow_summary['projectDir'] = workflow.projectDir - workflow_summary['userName'] = workflow.userName - workflow_summary['profile'] = workflow.profile - workflow_summary['configFiles'] = workflow.configFiles.join(', ') - - // Get pipeline parameters defined in JSON Schema - def Map params_summary = [:] - def blacklist = ['hostnames'] - def params_map = params_load(json_schema) - for (group in params_map.keySet()) { - def sub_params = new LinkedHashMap() - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (params.containsKey(param) && !blacklist.contains(param)) { - def params_value = params.get(param) - def schema_value = group_params.get(param).default - def param_type = group_params.get(param).type - if (schema_value == null) { - if (param_type == 'boolean') { - schema_value = false - } - if (param_type == 'string') { - schema_value = '' - } - if (param_type == 'integer') { - schema_value = 0 - } - } else { - if (param_type == 'string') { - if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { - def sub_string = schema_value.replace('\$projectDir','') - sub_string = sub_string.replace('\${projectDir}','') - if (params_value.contains(sub_string)) { - schema_value = params_value - } - } - if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { - def sub_string = schema_value.replace('\$params.outdir','') - sub_string = sub_string.replace('\${params.outdir}','') - if ("${params.outdir}${sub_string}" == params_value) { - schema_value = params_value - } - } - } - } - - if (params_value != schema_value) { - sub_params.put("$param", params_value) - } - } - } - params_summary.put(group, sub_params) - } - return [ 'Core Nextflow options' : workflow_summary ] << params_summary - } - - /* - * Beautify parameters for summary and return as string - */ - private static String params_summary_log(workflow, params, json_schema) { - String output = Headers.nf_core(workflow, params.monochrome_logs) + "\n" - def params_map = params_summary_map(workflow, params, json_schema) - def max_chars = params_max_chars(params_map) - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - if (group_params) { - output += group + "\n" - for (param in group_params.keySet()) { - output += " \u001B[1m" + param.padRight(max_chars) + ": \u001B[1m" + group_params.get(param) + "\n" - } - output += "\n" - } - } - output += Headers.dashed_line(params.monochrome_logs) - output += "\n\n" + Checks.citation(workflow) - output += "\n\n" + Headers.dashed_line(params.monochrome_logs) - return output - } - - static String params_summary_multiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } -} From 2867e56e52ef78aae4242547f523d9b65bbf6baf Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:08:21 +0000 Subject: [PATCH 204/344] Tidy up logic and push to workflows/pipeline.nf --- nf_core/pipeline-template/main.nf | 137 ++---------------------------- 1 file changed, 9 insertions(+), 128 deletions(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 9a43efd1da..78cf7f221e 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -15,13 +15,12 @@ nextflow.enable.dsl = 2 /* -- PRINT HELP -- */ //////////////////////////////////////////////////// -// log.info Headers.nf_core(workflow, params.monochrome_logs) +log.info Headers.nf_core(workflow, params.monochrome_logs) def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { // TODO nf-core: Update typical command used to run pipeline - def command = "nextflow run {{ name }} --input samplesheet.csv -profile docker" - //log.info Schema.params_help(workflow, params, json_schema, command) + def command = "nextflow run {{ name }} --input samplesheet.csv --genome GRCh37 -profile docker" log.info NfcoreSchema.params_help(workflow, params, json_schema, command) exit 0 } @@ -36,145 +35,27 @@ params.fasta = Checks.get_genome_attribute(params, 'fasta') /* -- PRINT PARAMETER SUMMARY -- */ //////////////////////////////////////////////////// -def summary_params = Schema.params_summary_map(workflow, params, json_schema) -log.info Schema.params_summary_log(workflow, params, json_schema) - -//////////////////////////////////////////////////// -/* -- PRINT PARAMETER SUMMARY -- */ -//////////////////////////////////////////////////// - -// log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) +def summary_params = NfcoreSchema.params_summary_map(workflow, params, json_schema) +log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) //////////////////////////////////////////////////// /* -- VALIDATE PARAMETERS -- */ //////////////////////////////////////////////////// -// if (params.validate_params) { -// NfcoreSchema.validateParameters(params, json_schema, log) -// } - -// Check that conda channels are set-up correctly -if (params.enable_conda) { - Checks.check_conda_channels(log) -} - -// Check AWS batch settings -Checks.aws_batch(workflow, params) - -// Check the hostnames against configured profiles -Checks.hostname(workflow, params, log) - -// Check genome key exists if provided -Checks.genome_exists(params, log) - -//////////////////////////////////////////////////// -/* -- VALIDATE INPUTS -- */ -//////////////////////////////////////////////////// - -// TODO nf-core: Add all file path parameters for the pipeline to the list below -// Check input path parameters to see if they exist -checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - -// Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } -if (params.fasta) { ch_fasta = file(params.fasta) } else { exit 1, 'Genome fasta file not specified!' } - -//////////////////////////////////////////////////// -/* -- CONFIG FILES -- */ -//////////////////////////////////////////////////// - -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() - -//////////////////////////////////////////////////// -/* -- IMPORT MODULES / SUBWORKFLOWS -- */ -//////////////////////////////////////////////////// - -// Don't overwrite global params.modules, create a copy instead and use that within the main script. -def modules = params.modules.clone() - -def multiqc_options = modules['multiqc'] -multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' - -// Local: Modules -include { GET_SOFTWARE_VERSIONS } from './modules/local/process/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) - -// Local: Sub-workflows -include { INPUT_CHECK } from './modules/local/subworkflow/input_check' addParams( options: [:] ) - -// nf-core/modules: Modules -include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from './modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) +Workflow.validate_main_params(workflow, params, json_schema, log) //////////////////////////////////////////////////// /* -- RUN MAIN WORKFLOW -- */ //////////////////////////////////////////////////// -// Info required for completion email and summary -def multiqc_report = [] - workflow { - - ch_software_versions = Channel.empty() - - /* - * SUBWORKFLOW: Read in samplesheet, validate and stage input files - */ - INPUT_CHECK ( - ch_input - ) - - /* - * MODULE: Run FastQC - */ - FASTQC ( - INPUT_CHECK.out.reads - ) - ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) - - /* - * MODULE: Pipeline reporting + * WORKFLOW: main pipeline workflow for {{ name }} */ - GET_SOFTWARE_VERSIONS ( - ch_software_versions.map { it }.collect() - ) - - /* - * MODULE: MultiQC - */ - workflow_summary = Schema.params_summary_multiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) - ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect() - ) - multiqc_report = MULTIQC.out.report.toList() - ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) + include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params ) + {{ short_name|upper }} () } -//////////////////////////////////////////////////// -/* -- COMPLETION EMAIL -- */ -//////////////////////////////////////////////////// - -workflow.onComplete { - Completion.email(workflow, params, summary_params, projectDir, log, multiqc_report) - Completion.summary(workflow, params, log) -} - -// workflow.onError { -// // Print unexpected parameters - easiest is to just rerun validation -// NfcoreSchema.validateParameters(params, json_schema, log) -// } - //////////////////////////////////////////////////// /* -- THE END -- */ -//////////////////////////////////////////////////// +//////////////////////////////////////////////////// \ No newline at end of file From ce1f0ea8d9c887063db66ea10b5bc645b53871a2 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:08:57 +0000 Subject: [PATCH 205/344] Remove failed Jinja2 workflow named file --- nf_core/pipeline-template/workflows/name | 0 nf_core/pipeline-template/workflows/{{ | 0 nf_core/pipeline-template/workflows/}}.nf | 0 3 files changed, 0 insertions(+), 0 deletions(-) delete mode 100644 nf_core/pipeline-template/workflows/name delete mode 100644 nf_core/pipeline-template/workflows/{{ delete mode 100644 nf_core/pipeline-template/workflows/}}.nf diff --git a/nf_core/pipeline-template/workflows/name b/nf_core/pipeline-template/workflows/name deleted file mode 100644 index e69de29bb2..0000000000 diff --git a/nf_core/pipeline-template/workflows/{{ b/nf_core/pipeline-template/workflows/{{ deleted file mode 100644 index e69de29bb2..0000000000 diff --git a/nf_core/pipeline-template/workflows/}}.nf b/nf_core/pipeline-template/workflows/}}.nf deleted file mode 100644 index e69de29bb2..0000000000 From 8ead7bc68189b732e86b2feb571f9bcdea0eca41 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:09:16 +0000 Subject: [PATCH 206/344] Add separate file for all Workflow.groovy functions --- nf_core/pipeline-template/lib/Workflow.groovy | 66 +++++++++++++++++++ 1 file changed, 66 insertions(+) create mode 100755 nf_core/pipeline-template/lib/Workflow.groovy diff --git a/nf_core/pipeline-template/lib/Workflow.groovy b/nf_core/pipeline-template/lib/Workflow.groovy new file mode 100755 index 0000000000..488f3dc12c --- /dev/null +++ b/nf_core/pipeline-template/lib/Workflow.groovy @@ -0,0 +1,66 @@ +/* + * This file holds several functions specific to the pipeline. + */ + +class Workflow { + + // Citation string + private static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " https://doi.org/10.5281/zenodo.1400710\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + static void validate_main_params(workflow, params, json_schema, log) { + if (params.validate_params) { + NfcoreSchema.validateParameters(params, json_schema, log) + } + + // Check that conda channels are set-up correctly + if (params.enable_conda) { + Checks.check_conda_channels(log) + } + + // Check AWS batch settings + Checks.aws_batch(workflow, params) + + // Check the hostnames against configured profiles + Checks.hostname(workflow, params, log) + } + + static void validate_workflow_params(params, log) { + genome_exists(params, log) + + if (!params.fasta) { + log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + System.exit(1) + } + } + + // Exit pipeline if incorrect --genome key provided + static void genome_exists(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "===================================================================================" + System.exit(1) + } + } + + // Get attribute from genome config file e.g. fasta + static String get_genome_attribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} From dc055608264aaae14f5a9092be827925032fecfa Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:09:38 +0000 Subject: [PATCH 207/344] Intial commit for main pipeline workflow --- .../pipeline-template/workflows/pipeline.nf | 127 ++++++++++++++++++ 1 file changed, 127 insertions(+) create mode 100644 nf_core/pipeline-template/workflows/pipeline.nf diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf new file mode 100644 index 0000000000..a0a71fdd44 --- /dev/null +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -0,0 +1,127 @@ +//////////////////////////////////////////////////// +/* -- LOCAL PARAMETER VALUES -- */ +//////////////////////////////////////////////////// + +params.summary_params = [:] + +//////////////////////////////////////////////////// +/* -- VALIDATE INPUTS -- */ +//////////////////////////////////////////////////// + +// Validate input parameters +Workflow.validate_workflow_params(params, log) + +// TODO nf-core: Add all file path parameters for the pipeline to the list below +// Check input path parameters to see if they exist +def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } + +//////////////////////////////////////////////////// +/* -- CONFIG FILES -- */ +//////////////////////////////////////////////////// + +ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() + +//////////////////////////////////////////////////// +/* -- IMPORT MODULES / SUBWORKFLOWS -- */ +//////////////////////////////////////////////////// + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. +def modules = params.modules.clone() + +def multiqc_options = modules['multiqc'] +multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' + +// Local: Modules +include { GET_SOFTWARE_VERSIONS } from './modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) + +// Local: Sub-workflows +include { INPUT_CHECK } from './subworkflows/local/input_check' addParams( options: [:] ) + +// nf-core/modules: Modules +include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from './modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) + +//////////////////////////////////////////////////// +/* -- RUN MAIN WORKFLOW -- */ +//////////////////////////////////////////////////// + +// Info required for completion email and summary +def multiqc_report = [] + +workflow {{ short_name|upper }} { + + ch_software_versions = Channel.empty() + + /* + * SUBWORKFLOW: Read in samplesheet, validate and stage input files + */ + INPUT_CHECK ( + ch_input + ) + + /* + * MODULE: Run FastQC + */ + FASTQC ( + INPUT_CHECK.out.reads + ) + ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) + + + /* + * MODULE: Pipeline reporting + */ + // Get unique list of files containing version information + ch_software_versions + .map { it -> if (it) [ it.baseName, it ] } + .groupTuple() + .map { it[1][0] } + .flatten() + .collect() + .set { ch_software_versions } + GET_SOFTWARE_VERSIONS ( + ch_software_versions + ) + + /* + * MODULE: MultiQC + */ + workflow_summary = NfcoreSchema.params_summary_multiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) + ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect() + ) + multiqc_report = MULTIQC.out.report.toList() + ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) +} + +//////////////////////////////////////////////////// +/* -- COMPLETION EMAIL -- */ +//////////////////////////////////////////////////// + +workflow.onComplete { + Completion.email(workflow, params, summary_params, projectDir, log, multiqc_report) + Completion.summary(workflow, params, log) +} + +// Print unexpected parameters - easiest is to just rerun validation +workflow.onError { + NfcoreSchema.validateParameters(params, json_schema, log) +} + +//////////////////////////////////////////////////// +/* -- THE END -- */ +//////////////////////////////////////////////////// From 9663d88462b3648cfaf1dd73cbe9026038b99371 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:28:54 +0000 Subject: [PATCH 208/344] Bump Nextflow version to 21.03.0-edge --- nf_core/pipeline-template/.github/workflows/ci.yml | 2 +- nf_core/pipeline-template/README.md | 2 +- nf_core/pipeline-template/nextflow.config | 4 ++-- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/.github/workflows/ci.yml b/nf_core/pipeline-template/.github/workflows/ci.yml index c81a6d10c4..90d4f866f0 100644 --- a/nf_core/pipeline-template/.github/workflows/ci.yml +++ b/nf_core/pipeline-template/.github/workflows/ci.yml @@ -20,7 +20,7 @@ jobs: strategy: matrix: # Nextflow versions: check pipeline minimum and current latest - nxf_ver: [20.11.0-edge] + nxf_ver: [21.03.0-edge] steps: - name: Check out pipeline code uses: actions/checkout@v2 diff --git a/nf_core/pipeline-template/README.md b/nf_core/pipeline-template/README.md index c74e02ee74..0f4ef1471e 100644 --- a/nf_core/pipeline-template/README.md +++ b/nf_core/pipeline-template/README.md @@ -5,7 +5,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/{{ short_name }}/results) [![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A520.11.0--edge-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.03.0--edge-23aa62.svg?labelColor=000000)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index 7abcc1a3f1..7f02e158f1 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -33,7 +33,7 @@ params { help = false validate_params = true show_hidden_params = false - schema_ignore_params = 'genomes' + schema_ignore_params = 'genomes,modules' enable_conda = false singularity_pull_docker_container = false @@ -162,7 +162,7 @@ manifest { homePage = 'https://github.com/{{ name }}' description = '{{ description }}' mainScript = 'main.nf' - nextflowVersion = '!>=20.11.0-edge' + nextflowVersion = '!>=21.03.0-edge' version = '{{ version }}' } From 6705723107f9944aa4df86af0ca564ad0f620049 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:29:09 +0000 Subject: [PATCH 209/344] Add plugins for AWS to igenomes.config --- nf_core/pipeline-template/conf/igenomes.config | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/nf_core/pipeline-template/conf/igenomes.config b/nf_core/pipeline-template/conf/igenomes.config index 7485867ba5..285a135199 100644 --- a/nf_core/pipeline-template/conf/igenomes.config +++ b/nf_core/pipeline-template/conf/igenomes.config @@ -418,3 +418,7 @@ params { } } } + +plugins { + id 'nf-amazon' +} \ No newline at end of file From 804b9d3659e53fd023488db3a55b36f84627349a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:29:28 +0000 Subject: [PATCH 210/344] Add genome parameter to test configs --- nf_core/pipeline-template/conf/test.config | 5 ++++- nf_core/pipeline-template/conf/test_full.config | 3 +++ 2 files changed, 7 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/conf/test.config b/nf_core/pipeline-template/conf/test.config index 3ded4a15a8..618445c947 100644 --- a/nf_core/pipeline-template/conf/test.config +++ b/nf_core/pipeline-template/conf/test.config @@ -19,8 +19,11 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + // Genome references + genome = 'R64-1-1' + // Ignore parameters that are expected to fail validation against Schema //schema_ignore_params = 'genomes' } diff --git a/nf_core/pipeline-template/conf/test_full.config b/nf_core/pipeline-template/conf/test_full.config index 1a37c32f25..8ec8675ddc 100644 --- a/nf_core/pipeline-template/conf/test_full.config +++ b/nf_core/pipeline-template/conf/test_full.config @@ -16,6 +16,9 @@ params { // TODO nf-core: Give any required params for the test so that command line flags are not needed input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + // Genome references + genome = 'R64-1-1' + // Ignore parameters that are expected to fail validation against Schema //schema_ignore_params = 'genomes' } From 6e48345d3040ff20483c3af67785ceee1a94fd69 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:29:45 +0000 Subject: [PATCH 211/344] Replace checks with Workflows --- nf_core/pipeline-template/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 78cf7f221e..23623f0f05 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -29,7 +29,7 @@ if (params.help) { /* -- GENOME PARAMETER VALUES -- */ //////////////////////////////////////////////////// -params.fasta = Checks.get_genome_attribute(params, 'fasta') +params.fasta = Workflow.get_genome_attribute(params, 'fasta') //////////////////////////////////////////////////// /* -- PRINT PARAMETER SUMMARY -- */ From b03b701db1f98a2b8a0d2db7379e212cb4f26537 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 18:45:21 +0000 Subject: [PATCH 212/344] Change module paths --- nf_core/pipeline-template/lib/Workflow.groovy | 0 nf_core/pipeline-template/workflows/pipeline.nf | 8 ++++---- 2 files changed, 4 insertions(+), 4 deletions(-) mode change 100755 => 100644 nf_core/pipeline-template/lib/Workflow.groovy diff --git a/nf_core/pipeline-template/lib/Workflow.groovy b/nf_core/pipeline-template/lib/Workflow.groovy old mode 100755 new mode 100644 diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index a0a71fdd44..fac295d011 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -37,14 +37,14 @@ def multiqc_options = modules['multiqc'] multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' // Local: Modules -include { GET_SOFTWARE_VERSIONS } from './modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) // Local: Sub-workflows -include { INPUT_CHECK } from './subworkflows/local/input_check' addParams( options: [:] ) +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) // nf-core/modules: Modules -include { FASTQC } from './modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from './modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) +include { FASTQC } from '../modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from '../modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) 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a/nf_core/create.py +++ b/nf_core/create.py @@ -83,7 +83,7 @@ def render_template(self): loader=jinja2.PackageLoader("nf_core", "pipeline-template"), keep_trailing_newline=True ) template_dir = os.path.join(os.path.dirname(__file__), "pipeline-template") - binary_ftypes = ["image", "application/java-archive"] + binary_ftypes = ["image", "application/java-archive", "application/x-java-archive"] object_attrs = vars(self) object_attrs["nf_core_version"] = nf_core.__version__ From c6a1684e647d9092bcabb38fc175b193087378b6 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:40:58 +0000 Subject: [PATCH 216/344] Remove --schema_ignore_params --- nf_core/pipeline-template/conf/test.config | 3 --- 1 file changed, 3 deletions(-) diff --git a/nf_core/pipeline-template/conf/test.config b/nf_core/pipeline-template/conf/test.config index 618445c947..fa00e312e5 100644 --- a/nf_core/pipeline-template/conf/test.config +++ b/nf_core/pipeline-template/conf/test.config @@ -23,7 +23,4 @@ params { // Genome references genome = 'R64-1-1' - - // Ignore parameters that are expected to fail validation against Schema - //schema_ignore_params = 'genomes' } From 480a355c90a2714bb008d9279d9a2f5afed90695 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:41:03 +0000 Subject: [PATCH 217/344] Remove --schema_ignore_params --- nf_core/pipeline-template/conf/test_full.config | 3 --- 1 file changed, 3 deletions(-) diff --git a/nf_core/pipeline-template/conf/test_full.config b/nf_core/pipeline-template/conf/test_full.config index 8ec8675ddc..3abd5287f6 100644 --- a/nf_core/pipeline-template/conf/test_full.config +++ b/nf_core/pipeline-template/conf/test_full.config @@ -18,7 +18,4 @@ params { // Genome references genome = 'R64-1-1' - - // Ignore parameters that are expected to fail validation against Schema - //schema_ignore_params = 'genomes' } From bce61f847fb9723d32fc17121e47f726db8d36c7 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:41:22 +0000 Subject: [PATCH 218/344] Add json_schema local param --- nf_core/pipeline-template/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 23623f0f05..47a112d9e0 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -52,7 +52,7 @@ workflow { /* * WORKFLOW: main pipeline workflow for {{ name }} */ - include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params ) + include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params, json_schema: json_schema ) {{ short_name|upper }} () } From f69b2a6ba7ca7a1530d0414422d35ac522becdfe Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:41:34 +0000 Subject: [PATCH 219/344] Rename reads variables --- nf_core/pipeline-template/subworkflows/local/input_check.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/subworkflows/local/input_check.nf b/nf_core/pipeline-template/subworkflows/local/input_check.nf index 8252fc31ce..5eb39c502f 100644 --- a/nf_core/pipeline-template/subworkflows/local/input_check.nf +++ b/nf_core/pipeline-template/subworkflows/local/input_check.nf @@ -14,10 +14,10 @@ workflow INPUT_CHECK { SAMPLESHEET_CHECK ( samplesheet ) .splitCsv ( header:true, sep:',' ) .map { get_sample_info(it) } - .set { sample_info } + .set { reads } emit: - sample_info // channel: [ val(meta), [ reads ] ] + reads // channel: [ val(meta), [ reads ] ] } // Function to get list of [ meta, [ fastq_1, fastq_2 ] ] From db3338299e088df6f0c05b3406a19f242dc45515 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:41:53 +0000 Subject: [PATCH 220/344] Add params.json_schema --- nf_core/pipeline-template/workflows/pipeline.nf | 11 +++++++---- 1 file changed, 7 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index fac295d011..809b04389b 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -3,6 +3,7 @@ //////////////////////////////////////////////////// params.summary_params = [:] +params.json_schema = '' //////////////////////////////////////////////////// /* -- VALIDATE INPUTS -- */ @@ -91,7 +92,7 @@ workflow {{ short_name|upper }} { /* * MODULE: MultiQC */ - workflow_summary = NfcoreSchema.params_summary_multiqc(workflow, summary_params) + workflow_summary = NfcoreSchema.params_summary_multiqc(workflow, params.summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty() @@ -113,13 +114,15 @@ workflow {{ short_name|upper }} { //////////////////////////////////////////////////// workflow.onComplete { - Completion.email(workflow, params, summary_params, projectDir, log, multiqc_report) + Completion.email(workflow, params, params.summary_params, projectDir, log, multiqc_report) Completion.summary(workflow, params, log) } // Print unexpected parameters - easiest is to just rerun validation -workflow.onError { - NfcoreSchema.validateParameters(params, json_schema, log) +if (params.json_schema) {} + workflow.onError { + NfcoreSchema.validateParameters(params, params.json_schema, log) + } } //////////////////////////////////////////////////// From 5be78f881b5c0b593e3a0d2ef4550c5b020c6be3 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:48:22 +0000 Subject: [PATCH 221/344] Use native variable values for initialisation in nextflow.config --- nf_core/pipeline-template/nextflow.config | 22 +++++++++++----------- 1 file changed, 11 insertions(+), 11 deletions(-) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index 7f02e158f1..4b79b2b261 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -10,24 +10,24 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options - input = null + input = '' // References - genome = null + genome = '' igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false // MultiQC options - multiqc_config = null - multiqc_title = null + multiqc_config = '' + multiqc_title = '' max_multiqc_email_size = 25.MB // Boilerplate options outdir = './results' tracedir = "${params.outdir}/pipeline_info" publish_dir_mode = 'copy' - email = null - email_on_fail = null + email = '' + email_on_fail = '' plaintext_email = false monochrome_logs = false help = false @@ -40,11 +40,11 @@ params { // Config options custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = null - config_profile_description = null - config_profile_contact = null - config_profile_url = null - config_profile_name = null + hostnames = [:] + config_profile_description = '' + config_profile_contact = '' + config_profile_url = '' + config_profile_name = '' // Max resource options // Defaults only, expecting to be overwritten From 4e1ebfb053c72903a69ba88161df4134d4a5e8cb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:51:53 +0000 Subject: [PATCH 222/344] Rewrite comments --- nf_core/pipeline-template/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 47a112d9e0..350876a449 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -50,7 +50,7 @@ Workflow.validate_main_params(workflow, params, json_schema, log) workflow { /* - * WORKFLOW: main pipeline workflow for {{ name }} + * WORKFLOW: Main pipeline workflow for {{ name }} */ include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params, json_schema: json_schema ) {{ short_name|upper }} () From ca266050c7d8f971512ac6171be4b89ffe067ba9 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 19:52:02 +0000 Subject: [PATCH 223/344] Rewrite comments --- nf_core/pipeline-template/workflows/pipeline.nf | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index 809b04389b..f68e498506 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -37,16 +37,16 @@ def modules = params.modules.clone() def multiqc_options = modules['multiqc'] multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' -// Local: Modules +// Modules: local include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) -// Local: Sub-workflows -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) - -// nf-core/modules: Modules +// Modules: nf-core/modules include { FASTQC } from '../modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) include { MULTIQC } from '../modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) +// Subworkflows: local +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) + //////////////////////////////////////////////////// /* -- RUN MAIN WORKFLOW -- */ //////////////////////////////////////////////////// @@ -119,7 +119,7 @@ workflow.onComplete { } // Print unexpected parameters - easiest is to just rerun validation -if (params.json_schema) {} +if (params.json_schema) { workflow.onError { NfcoreSchema.validateParameters(params, params.json_schema, log) } From 6e226b3b775ca3daee279bd4eec7ccd80cf349a1 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 20:02:23 +0000 Subject: [PATCH 224/344] Remove json_schema param --- nf_core/pipeline-template/main.nf | 2 +- nf_core/pipeline-template/workflows/pipeline.nf | 8 -------- 2 files changed, 1 insertion(+), 9 deletions(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 350876a449..814083d784 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -52,7 +52,7 @@ workflow { /* * WORKFLOW: Main pipeline workflow for {{ name }} */ - include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params, json_schema: json_schema ) + include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params ) {{ short_name|upper }} () } diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index f68e498506..44c3d01806 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -3,7 +3,6 @@ //////////////////////////////////////////////////// params.summary_params = [:] -params.json_schema = '' //////////////////////////////////////////////////// /* -- VALIDATE INPUTS -- */ @@ -118,13 +117,6 @@ workflow.onComplete { Completion.summary(workflow, params, log) } -// Print unexpected parameters - easiest is to just rerun validation -if (params.json_schema) { - workflow.onError { - NfcoreSchema.validateParameters(params, params.json_schema, log) - } -} - //////////////////////////////////////////////////// /* -- THE END -- */ //////////////////////////////////////////////////// From 0fc7572eb8f0516b1f75c69577731a04ebb7920f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 20:02:52 +0000 Subject: [PATCH 225/344] Reword log summary message --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index f4094ba632..20bc9fa349 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -562,7 +562,7 @@ class NfcoreSchema { output += '\n' } } - output += "[Only displaying parameters that differ from pipeline default]\n" + output += "[Only displaying parameters that differ from the pipeline defaults]\n" output += dashed_line(params.monochrome_logs) output += '\n\n' + dashed_line(params.monochrome_logs) return output From a05fdcf26d7e34fd4a24376e66c61b4af53bfca8 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 20:37:31 +0000 Subject: [PATCH 226/344] Remove unnessary dashed line --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 20bc9fa349..4076edd9a8 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -564,7 +564,6 @@ class NfcoreSchema { } output += "[Only displaying parameters that differ from the pipeline defaults]\n" output += dashed_line(params.monochrome_logs) - output += '\n\n' + dashed_line(params.monochrome_logs) return output } From b9531e9183b9181e5491e112ec4029f2d8144101 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 20:40:30 +0000 Subject: [PATCH 227/344] Add comments for using Zenodo DOI --- nf_core/pipeline-template/lib/Workflow.groovy | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/lib/Workflow.groovy b/nf_core/pipeline-template/lib/Workflow.groovy index 488f3dc12c..a4880d7de2 100644 --- a/nf_core/pipeline-template/lib/Workflow.groovy +++ b/nf_core/pipeline-template/lib/Workflow.groovy @@ -7,8 +7,9 @@ class Workflow { // Citation string private static String citation(workflow) { return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - " https://doi.org/10.5281/zenodo.1400710\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + From cce81184755d4beb85d3968824d2424d28f77cb7 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 20:40:49 +0000 Subject: [PATCH 228/344] Add dashed lines after logs for help and summary --- nf_core/pipeline-template/main.nf | 9 +++++---- 1 file changed, 5 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 814083d784..9a366fa9b6 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -22,6 +22,8 @@ if (params.help) { // TODO nf-core: Update typical command used to run pipeline def command = "nextflow run {{ name }} --input samplesheet.csv --genome GRCh37 -profile docker" log.info NfcoreSchema.params_help(workflow, params, json_schema, command) + log.info Worflow.citation(workflow) + log.info NfcoreSchema.dashed_line(params.monochrome_logs) exit 0 } @@ -37,6 +39,8 @@ params.fasta = Workflow.get_genome_attribute(params, 'fasta') def summary_params = NfcoreSchema.params_summary_map(workflow, params, json_schema) log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) +log.info Worflow.citation(workflow) +log.info NfcoreSchema.dashed_line(params.monochrome_logs) //////////////////////////////////////////////////// /* -- VALIDATE PARAMETERS -- */ @@ -45,13 +49,10 @@ log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) Workflow.validate_main_params(workflow, params, json_schema, log) //////////////////////////////////////////////////// -/* -- RUN MAIN WORKFLOW -- */ +/* -- RUN WORKFLOW(S) -- */ //////////////////////////////////////////////////// workflow { - /* - * WORKFLOW: Main pipeline workflow for {{ name }} - */ include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params ) {{ short_name|upper }} () } From c90f03ac9f9d00f7c693fe7eeba87ad84b3dabec Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 20:55:00 +0000 Subject: [PATCH 229/344] Refine summary log info and help --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 5 ++--- nf_core/pipeline-template/main.nf | 4 ++-- 2 files changed, 4 insertions(+), 5 deletions(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 4076edd9a8..9a2f7cf976 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -463,11 +463,10 @@ class NfcoreSchema { output += group_output } } - output += dashed_line(params.monochrome_logs) if (num_hidden > 0){ - output += colors.dim + "\n Hiding $num_hidden params, use --show_hidden_params to show.\n" + colors.reset - output += dashed_line(params.monochrome_logs) + output += colors.dim + "\n [Hiding $num_hidden params, use --show_hidden_params to show.]\n" + colors.reset } + output += dashed_line(params.monochrome_logs) return output } diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 9a366fa9b6..b21e224cf8 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -22,7 +22,7 @@ if (params.help) { // TODO nf-core: Update typical command used to run pipeline def command = "nextflow run {{ name }} --input samplesheet.csv --genome GRCh37 -profile docker" log.info NfcoreSchema.params_help(workflow, params, json_schema, command) - log.info Worflow.citation(workflow) + log.info Workflow.citation(workflow) log.info NfcoreSchema.dashed_line(params.monochrome_logs) exit 0 } @@ -39,7 +39,7 @@ params.fasta = Workflow.get_genome_attribute(params, 'fasta') def summary_params = NfcoreSchema.params_summary_map(workflow, params, json_schema) log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) -log.info Worflow.citation(workflow) +log.info Workflow.citation(workflow) log.info NfcoreSchema.dashed_line(params.monochrome_logs) //////////////////////////////////////////////////// From bfe06666afb8c3481173158061259b59477abf2a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 21:21:21 +0000 Subject: [PATCH 230/344] Adjust spacing in param printing --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 9a2f7cf976..8f8e576191 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -464,7 +464,7 @@ class NfcoreSchema { } } if (num_hidden > 0){ - output += colors.dim + "\n [Hiding $num_hidden params, use --show_hidden_params to show.]\n" + colors.reset + output += colors.dim + "[ Hiding $num_hidden params, use --show_hidden_params to show them ]\n" + colors.reset } output += dashed_line(params.monochrome_logs) return output @@ -561,7 +561,7 @@ class NfcoreSchema { output += '\n' } } - output += "[Only displaying parameters that differ from the pipeline defaults]\n" + output += "[ Only displaying parameters that differ from the pipeline defaults ]\n" output += dashed_line(params.monochrome_logs) return output } From d8aaca4c15ce3fbf68bee2448c50bf291e4bd43f Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 21:52:31 +0000 Subject: [PATCH 231/344] use camelCase for function names in Groovy scripts --- nf_core/pipeline-template/lib/Checks.groovy | 6 +- nf_core/pipeline-template/lib/Headers.groovy | 43 -------- .../pipeline-template/lib/NfcoreSchema.groovy | 104 +++--------------- nf_core/pipeline-template/lib/Utils.groovy | 92 ++++++++++++++++ nf_core/pipeline-template/lib/Workflow.groovy | 16 +-- nf_core/pipeline-template/main.nf | 16 +-- .../pipeline-template/workflows/pipeline.nf | 2 +- 7 files changed, 129 insertions(+), 150 deletions(-) delete mode 100644 nf_core/pipeline-template/lib/Headers.groovy create mode 100644 nf_core/pipeline-template/lib/Utils.groovy diff --git a/nf_core/pipeline-template/lib/Checks.groovy b/nf_core/pipeline-template/lib/Checks.groovy index 01d9d4bb57..a120044b89 100644 --- a/nf_core/pipeline-template/lib/Checks.groovy +++ b/nf_core/pipeline-template/lib/Checks.groovy @@ -6,7 +6,7 @@ import org.yaml.snakeyaml.Yaml class Checks { - static void check_conda_channels(log) { + static void checkCondaChannels(log) { Yaml parser = new Yaml() def channels = [] try { @@ -35,7 +35,7 @@ class Checks { } } - static void aws_batch(workflow, params) { + static void awsBatch(workflow, params) { if (workflow.profile.contains('awsbatch')) { assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" // Check outdir paths to be S3 buckets if running on AWSBatch @@ -46,7 +46,7 @@ class Checks { } } - static void hostname(workflow, params, log) { + static void hostName(workflow, params, log) { Map colors = Headers.log_colours(params.monochrome_logs) if (params.hostnames) { def hostname = "hostname".execute().text.trim() diff --git a/nf_core/pipeline-template/lib/Headers.groovy b/nf_core/pipeline-template/lib/Headers.groovy deleted file mode 100644 index 15d1d38800..0000000000 --- a/nf_core/pipeline-template/lib/Headers.groovy +++ /dev/null @@ -1,43 +0,0 @@ -/* - * This file holds several functions used to render the nf-core ANSI header. - */ - -class Headers { - - private static Map log_colours(Boolean monochrome_logs) { - Map colorcodes = [:] - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['yellow_bold'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - colorcodes['red'] = monochrome_logs ? '' : "\033[1;91m" - return colorcodes - } - - static String dashed_line(monochrome_logs) { - Map colors = log_colours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - static String nf_core(workflow, monochrome_logs) { - Map colors = log_colours(monochrome_logs) - String.format( - """\n - ${dashed_line(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} - ${dashed_line(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 8f8e576191..0998053a40 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -148,7 +148,7 @@ class NfcoreSchema { // Check for unexpected parameters if (unexpectedParams.size() > 0) { - Map colors = log_colours(params.monochrome_logs) + Map colors = Utils.logColours(params.monochrome_logs) println '' def warn_msg = 'Found unexpected parameters:' for (unexpectedParam in unexpectedParams) { @@ -256,10 +256,10 @@ class NfcoreSchema { /* * This method tries to read a JSON params file */ - private static LinkedHashMap params_load(String json_schema) { + private static LinkedHashMap paramsLoad(String json_schema) { def params_map = new LinkedHashMap() try { - params_map = params_read(json_schema) + params_map = paramsRead(json_schema) } catch (Exception e) { println "Could not read parameters settings from JSON. $e" params_map = new LinkedHashMap() @@ -267,76 +267,6 @@ class NfcoreSchema { return params_map } - private static Map log_colours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - static String dashed_line(monochrome_logs) { - Map colors = log_colours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - /* Method to actually read in JSON file using Groovy. Group (as Key), values are all parameters @@ -346,7 +276,7 @@ class NfcoreSchema { Group - */ - private static LinkedHashMap params_read(String json_schema) throws Exception { + private static LinkedHashMap paramsRead(String json_schema) throws Exception { def json = new File(json_schema).text def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') @@ -400,7 +330,7 @@ class NfcoreSchema { /* * Get maximum number of characters across all parameter names */ - private static Integer params_max_chars(params_map) { + private static Integer paramsMaxChars(params_map) { Integer max_chars = 0 for (group in params_map.keySet()) { def group_params = params_map.get(group) // This gets the parameters of that particular group @@ -416,14 +346,14 @@ class NfcoreSchema { /* * Beautify parameters for --help */ - private static String params_help(workflow, params, json_schema, command) { - Map colors = log_colours(params.monochrome_logs) + private static String paramsHelp(workflow, params, json_schema, command) { + Map colors = Utils.logColours(params.monochrome_logs) Integer num_hidden = 0 String output = '' output += 'Typical pipeline command:\n\n' output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = params_load(json_schema) - Integer max_chars = params_max_chars(params_map) + 1 + Map params_map = paramsLoad(json_schema) + Integer max_chars = paramsMaxChars(params_map) + 1 Integer desc_indent = max_chars + 14 Integer dec_linewidth = 160 - desc_indent for (group in params_map.keySet()) { @@ -466,14 +396,14 @@ class NfcoreSchema { if (num_hidden > 0){ output += colors.dim + "[ Hiding $num_hidden params, use --show_hidden_params to show them ]\n" + colors.reset } - output += dashed_line(params.monochrome_logs) + output += Utils.dashedLine(params.monochrome_logs) return output } /* * Groovy Map summarising parameters/workflow options used by the pipeline */ - private static LinkedHashMap params_summary_map(workflow, params, json_schema) { + private static LinkedHashMap paramsSummaryMap(workflow, params, json_schema) { // Get a selection of core Nextflow workflow options def Map workflow_summary = [:] if (workflow.revision) { @@ -496,7 +426,7 @@ class NfcoreSchema { // Get pipeline parameters defined in JSON Schema def Map params_summary = [:] def blacklist = ['hostnames'] - def params_map = params_load(json_schema) + def params_map = paramsLoad(json_schema) for (group in params_map.keySet()) { def sub_params = new LinkedHashMap() def group_params = params_map.get(group) // This gets the parameters of that particular group @@ -547,10 +477,10 @@ class NfcoreSchema { /* * Beautify parameters for summary and return as string */ - private static String params_summary_log(workflow, params, json_schema) { + private static String paramsSummaryLog(workflow, params, json_schema) { String output = '' - def params_map = params_summary_map(workflow, params, json_schema) - def max_chars = params_max_chars(params_map) + def params_map = paramsSummaryMap(workflow, params, json_schema) + def max_chars = paramsMaxChars(params_map) for (group in params_map.keySet()) { def group_params = params_map.get(group) // This gets the parameters of that particular group if (group_params) { @@ -562,14 +492,14 @@ class NfcoreSchema { } } output += "[ Only displaying parameters that differ from the pipeline defaults ]\n" - output += dashed_line(params.monochrome_logs) + output += Utils.dashedLine(params.monochrome_logs) return output } /* * Get Workflow summary for MultiQC */ - static String params_summary_multiqc(workflow, summary) { + static String paramsSummaryMultiqc(workflow, summary) { String summary_section = '' for (group in summary.keySet()) { def group_params = summary.get(group) // This gets the parameters of that particular group diff --git a/nf_core/pipeline-template/lib/Utils.groovy b/nf_core/pipeline-template/lib/Utils.groovy new file mode 100644 index 0000000000..10b1ac6109 --- /dev/null +++ b/nf_core/pipeline-template/lib/Utils.groovy @@ -0,0 +1,92 @@ +/* + * This file holds several Groovy functions used across the nf-core pipeline template + */ + +class Utils { + private static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + static String dashedLine(monochrome_logs) { + Map colors = log_colours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + static String nfcore(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } + +} \ No newline at end of file diff --git a/nf_core/pipeline-template/lib/Workflow.groovy b/nf_core/pipeline-template/lib/Workflow.groovy index a4880d7de2..02c79344da 100644 --- a/nf_core/pipeline-template/lib/Workflow.groovy +++ b/nf_core/pipeline-template/lib/Workflow.groovy @@ -16,25 +16,25 @@ class Workflow { " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } - static void validate_main_params(workflow, params, json_schema, log) { + static void validateMainParams(workflow, params, json_schema, log) { if (params.validate_params) { NfcoreSchema.validateParameters(params, json_schema, log) } // Check that conda channels are set-up correctly if (params.enable_conda) { - Checks.check_conda_channels(log) + Checks.checkCondaChannels(log) } // Check AWS batch settings - Checks.aws_batch(workflow, params) + Checks.awsBatch(workflow, params) // Check the hostnames against configured profiles - Checks.hostname(workflow, params, log) + Checks.hostName(workflow, params, log) } - static void validate_workflow_params(params, log) { - genome_exists(params, log) + static void validateWorkflowParams(params, log) { + genomeExists(params, log) if (!params.fasta) { log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." @@ -43,7 +43,7 @@ class Workflow { } // Exit pipeline if incorrect --genome key provided - static void genome_exists(params, log) { + static void genomeExists(params, log) { if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { log.error "=============================================================================\n" + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + @@ -55,7 +55,7 @@ class Workflow { } // Get attribute from genome config file e.g. fasta - static String get_genome_attribute(params, attribute) { + static String getGenomeAttribute(params, attribute) { def val = '' if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { if (params.genomes[ params.genome ].containsKey(attribute)) { diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index b21e224cf8..f2a4757452 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -15,15 +15,15 @@ nextflow.enable.dsl = 2 /* -- PRINT HELP -- */ //////////////////////////////////////////////////// -log.info Headers.nf_core(workflow, params.monochrome_logs) +log.info Utils.nfcore(workflow, params.monochrome_logs) def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { // TODO nf-core: Update typical command used to run pipeline def command = "nextflow run {{ name }} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info NfcoreSchema.params_help(workflow, params, json_schema, command) + log.info NfcoreSchema.paramsHelp(workflow, params, json_schema, command) log.info Workflow.citation(workflow) - log.info NfcoreSchema.dashed_line(params.monochrome_logs) + log.info Utils.dashedLine(params.monochrome_logs) exit 0 } @@ -31,22 +31,22 @@ if (params.help) { /* -- GENOME PARAMETER VALUES -- */ //////////////////////////////////////////////////// -params.fasta = Workflow.get_genome_attribute(params, 'fasta') +params.fasta = Workflow.getGenomeAttribute(params, 'fasta') //////////////////////////////////////////////////// /* -- PRINT PARAMETER SUMMARY -- */ //////////////////////////////////////////////////// -def summary_params = NfcoreSchema.params_summary_map(workflow, params, json_schema) -log.info NfcoreSchema.params_summary_log(workflow, params, json_schema) +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params, json_schema) +log.info NfcoreSchema.paramsSummaryLog(workflow, params, json_schema) log.info Workflow.citation(workflow) -log.info NfcoreSchema.dashed_line(params.monochrome_logs) +log.info NfcoreSchema.dashedLine(params.monochrome_logs) //////////////////////////////////////////////////// /* -- VALIDATE PARAMETERS -- */ //////////////////////////////////////////////////// -Workflow.validate_main_params(workflow, params, json_schema, log) +Workflow.validateMainParams(workflow, params, json_schema, log) //////////////////////////////////////////////////// /* -- RUN WORKFLOW(S) -- */ diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index 44c3d01806..71f8ee3b42 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -9,7 +9,7 @@ params.summary_params = [:] //////////////////////////////////////////////////// // Validate input parameters -Workflow.validate_workflow_params(params, log) +Workflow.validateWorkflowParams(params, log) // TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist From 2da6182eb2399e07979ba576f02d2868e3eaf8bb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 22:07:41 +0000 Subject: [PATCH 232/344] Change Groovy function names --- nf_core/pipeline-template/lib/Checks.groovy | 2 +- nf_core/pipeline-template/lib/Completion.groovy | 6 +++--- nf_core/pipeline-template/lib/Utils.groovy | 2 +- nf_core/pipeline-template/main.nf | 2 +- 4 files changed, 6 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/lib/Checks.groovy b/nf_core/pipeline-template/lib/Checks.groovy index a120044b89..027deda5c1 100644 --- a/nf_core/pipeline-template/lib/Checks.groovy +++ b/nf_core/pipeline-template/lib/Checks.groovy @@ -47,7 +47,7 @@ class Checks { } static void hostName(workflow, params, log) { - Map colors = Headers.log_colours(params.monochrome_logs) + Map colors = Utils.logColours(params.monochrome_logs) if (params.hostnames) { def hostname = "hostname".execute().text.trim() params.hostnames.each { prof, hnames -> diff --git a/nf_core/pipeline-template/lib/Completion.groovy b/nf_core/pipeline-template/lib/Completion.groovy index f6395a3599..968d5a47ac 100644 --- a/nf_core/pipeline-template/lib/Completion.groovy +++ b/nf_core/pipeline-template/lib/Completion.groovy @@ -82,7 +82,7 @@ class Completion { def sendmail_html = sendmail_template.toString() // Send the HTML e-mail - Map colors = Headers.log_colours(params.monochrome_logs) + Map colors = Utils.logColours(params.monochrome_logs) if (email_address) { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } @@ -112,7 +112,7 @@ class Completion { } static void summary(workflow, params, log) { - Map colors = Headers.log_colours(params.monochrome_logs) + Map colors = Utils.logColours(params.monochrome_logs) if (workflow.success) { if (workflow.stats.ignoredCount == 0) { log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" @@ -120,7 +120,7 @@ class Completion { log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" } } else { - Checks.hostname(workflow, params, log) + Checks.hostName(workflow, params, log) log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" } } diff --git a/nf_core/pipeline-template/lib/Utils.groovy b/nf_core/pipeline-template/lib/Utils.groovy index 10b1ac6109..51898b832d 100644 --- a/nf_core/pipeline-template/lib/Utils.groovy +++ b/nf_core/pipeline-template/lib/Utils.groovy @@ -73,7 +73,7 @@ class Utils { return "-${colors.dim}----------------------------------------------------${colors.reset}-" } - static String nfcore(workflow, monochrome_logs) { + static String logo(workflow, monochrome_logs) { Map colors = logColours(monochrome_logs) String.format( """\n diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index f2a4757452..8feb434b3a 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -15,7 +15,7 @@ nextflow.enable.dsl = 2 /* -- PRINT HELP -- */ //////////////////////////////////////////////////// -log.info Utils.nfcore(workflow, params.monochrome_logs) +log.info Utils.logo(workflow, params.monochrome_logs) def json_schema = "$projectDir/nextflow_schema.json" if (params.help) { From 18b49181b85a589b2c130e8fd0600a8f9933a4ff Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 22:09:22 +0000 Subject: [PATCH 233/344] Use brand spanking new Utils.groovy --- nf_core/pipeline-template/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index 8feb434b3a..c62f7e0e18 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -40,7 +40,7 @@ params.fasta = Workflow.getGenomeAttribute(params, 'fasta') def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params, json_schema) log.info NfcoreSchema.paramsSummaryLog(workflow, params, json_schema) log.info Workflow.citation(workflow) -log.info NfcoreSchema.dashedLine(params.monochrome_logs) +log.info Utils.dashedLine(params.monochrome_logs) //////////////////////////////////////////////////// /* -- VALIDATE PARAMETERS -- */ From c1a410bfe23076ff0dd8a065f9b69516c0f9727a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 22:17:49 +0000 Subject: [PATCH 234/344] Fix log_colours to logColours --- nf_core/pipeline-template/lib/Utils.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/lib/Utils.groovy b/nf_core/pipeline-template/lib/Utils.groovy index 51898b832d..558f9707e0 100644 --- a/nf_core/pipeline-template/lib/Utils.groovy +++ b/nf_core/pipeline-template/lib/Utils.groovy @@ -69,7 +69,7 @@ class Utils { } static String dashedLine(monochrome_logs) { - Map colors = log_colours(monochrome_logs) + Map colors = logColours(monochrome_logs) return "-${colors.dim}----------------------------------------------------${colors.reset}-" } From a7cd8bb422df9516ec05b34bfd317e4031328f57 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Sun, 21 Mar 2021 22:36:15 +0000 Subject: [PATCH 235/344] Fix Groovy paths --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 4 ++-- nf_core/pipeline-template/workflows/pipeline.nf | 4 ++-- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 0998053a40..1be9ae77aa 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -394,7 +394,7 @@ class NfcoreSchema { } } if (num_hidden > 0){ - output += colors.dim + "[ Hiding $num_hidden params, use --show_hidden_params to show them ]\n" + colors.reset + output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset } output += Utils.dashedLine(params.monochrome_logs) return output @@ -491,7 +491,7 @@ class NfcoreSchema { output += '\n' } } - output += "[ Only displaying parameters that differ from the pipeline defaults ]\n" + output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" output += Utils.dashedLine(params.monochrome_logs) return output } diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index 71f8ee3b42..c75e02b629 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -91,7 +91,7 @@ workflow {{ short_name|upper }} { /* * MODULE: MultiQC */ - workflow_summary = NfcoreSchema.params_summary_multiqc(workflow, params.summary_params) + workflow_summary = NfcoreSchema.paramsSummaryMultiqc(workflow, params.summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty() @@ -119,4 +119,4 @@ workflow.onComplete { //////////////////////////////////////////////////// /* -- THE END -- */ -//////////////////////////////////////////////////// +//////////////////////////////////////////////////// \ No newline at end of file From 9de5995b5778844c15215de3fc8770bf2e48be13 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 14:16:59 +0000 Subject: [PATCH 236/344] Bump Nextflow version to 21.03.0-edge for pytest too --- .github/workflows/pytest.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.github/workflows/pytest.yml b/.github/workflows/pytest.yml index 493fb53c20..6fb6dd7ddd 100644 --- a/.github/workflows/pytest.yml +++ b/.github/workflows/pytest.yml @@ -33,6 +33,7 @@ jobs: - name: Install Nextflow env: + NXF_VER: 21.03.0-edge CAPSULE_LOG: none run: | mkdir /tmp/nextflow From 13299b39b20a58c6c291a7ebf5d18c5d1767dca8 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 14:39:36 +0000 Subject: [PATCH 237/344] Export NXF_VER manually --- .github/workflows/pytest.yml | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/.github/workflows/pytest.yml b/.github/workflows/pytest.yml index 6fb6dd7ddd..624cd21dc4 100644 --- a/.github/workflows/pytest.yml +++ b/.github/workflows/pytest.yml @@ -31,11 +31,13 @@ jobs: python -m pip install --upgrade pip -r requirements-dev.txt pip install -e . + - name: Install Nextflow env: - NXF_VER: 21.03.0-edge + #NXF_VER: "21.03.0-edge" CAPSULE_LOG: none run: | + export NXF_VER="21.03.0-edge" mkdir /tmp/nextflow cd /tmp/nextflow wget -qO- get.nextflow.io | bash From 50f8a679381869ea0e21489391a849f917c7dd62 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 14:42:27 +0000 Subject: [PATCH 238/344] Export NXF_VER before pytest --- .github/workflows/pytest.yml | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/.github/workflows/pytest.yml b/.github/workflows/pytest.yml index 624cd21dc4..2c89caba3b 100644 --- a/.github/workflows/pytest.yml +++ b/.github/workflows/pytest.yml @@ -31,19 +31,18 @@ jobs: python -m pip install --upgrade pip -r requirements-dev.txt pip install -e . - - name: Install Nextflow env: - #NXF_VER: "21.03.0-edge" CAPSULE_LOG: none run: | - export NXF_VER="21.03.0-edge" mkdir /tmp/nextflow cd /tmp/nextflow wget -qO- get.nextflow.io | bash sudo ln -s /tmp/nextflow/nextflow /usr/local/bin/nextflow - name: Test with pytest + env: + NXF_VER: "21.03.0-edge" run: python3 -m pytest --color=yes --cov-report=xml --cov-config=.github/.coveragerc --cov=nf_core - uses: codecov/codecov-action@v1 From 5ba0d710b49a5c029303d8612ebb2f0f89db9f12 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 14:44:25 +0000 Subject: [PATCH 239/344] Move MultiQC function to Workflow.groovy --- .../pipeline-template/lib/NfcoreSchema.groovy | 27 ----------------- nf_core/pipeline-template/lib/Workflow.groovy | 29 ++++++++++++++++++- .../pipeline-template/workflows/pipeline.nf | 2 +- 3 files changed, 29 insertions(+), 29 deletions(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 1be9ae77aa..1d6d36072e 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -495,31 +495,4 @@ class NfcoreSchema { output += Utils.dashedLine(params.monochrome_logs) return output } - - /* - * Get Workflow summary for MultiQC - */ - static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } } diff --git a/nf_core/pipeline-template/lib/Workflow.groovy b/nf_core/pipeline-template/lib/Workflow.groovy index 02c79344da..d28dcc6b3b 100644 --- a/nf_core/pipeline-template/lib/Workflow.groovy +++ b/nf_core/pipeline-template/lib/Workflow.groovy @@ -63,5 +63,32 @@ class Workflow { } } return val - } + } + + /* + * Get workflow summary for MultiQC + */ + static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "
\n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } } diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index c75e02b629..ad50691d24 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -91,7 +91,7 @@ workflow {{ short_name|upper }} { /* * MODULE: MultiQC */ - workflow_summary = NfcoreSchema.paramsSummaryMultiqc(workflow, params.summary_params) + workflow_summary = Workflow.paramsSummaryMultiqc(workflow, params.summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty() From 2af85103dfe655926c0db0898183086a2d4767fb Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 16:30:48 +0000 Subject: [PATCH 240/344] Move NXF_VER to job level --- .github/workflows/pytest.yml | 71 ++++++++++++++++++++---------------- 1 file changed, 39 insertions(+), 32 deletions(-) diff --git a/.github/workflows/pytest.yml b/.github/workflows/pytest.yml index 2c89caba3b..83bc3213d6 100644 --- a/.github/workflows/pytest.yml +++ b/.github/workflows/pytest.yml @@ -13,40 +13,47 @@ jobs: pytest: runs-on: ubuntu-latest + env: + NXF_VER: 21.03.0-edge strategy: matrix: python-version: [3.6, 3.7, 3.8] steps: - - uses: actions/checkout@v2 - name: Check out source-code repository - - - name: Set up Python ${{ matrix.python-version }} - uses: actions/setup-python@v1 - with: - python-version: ${{ matrix.python-version }} - - - name: Install python dependencies - run: | - python -m pip install --upgrade pip -r requirements-dev.txt - pip install -e . - - - name: Install Nextflow - env: - CAPSULE_LOG: none - run: | - mkdir /tmp/nextflow - cd /tmp/nextflow - wget -qO- get.nextflow.io | bash - sudo ln -s /tmp/nextflow/nextflow /usr/local/bin/nextflow - - - name: Test with pytest - env: - NXF_VER: "21.03.0-edge" - run: python3 -m pytest --color=yes --cov-report=xml --cov-config=.github/.coveragerc --cov=nf_core - - - uses: codecov/codecov-action@v1 - name: Upload code coverage report - with: - if: success() - token: ${{ secrets.CODECOV_TOKEN }} + - uses: actions/checkout@v2 + name: Check out source-code repository + + - name: Set up Python ${{ matrix.python-version }} + uses: actions/setup-python@v1 + with: + python-version: ${{ matrix.python-version }} + + - name: Install python dependencies + run: | + python -m pip install --upgrade pip -r requirements-dev.txt + pip install -e . + + - name: Install Nextflow + env: + CAPSULE_LOG: none + run: | + wget -qO- get.nextflow.io | bash + sudo mv nextflow /usr/local/bin/ + + # - name: Install Nextflow + # env: + # CAPSULE_LOG: none + # run: | + # mkdir /tmp/nextflow + # cd /tmp/nextflow + # wget -qO- get.nextflow.io | bash + # sudo ln -s /tmp/nextflow/nextflow /usr/local/bin/nextflow + + - name: Test with pytest + run: python3 -m pytest --color=yes --cov-report=xml --cov-config=.github/.coveragerc --cov=nf_core + + - uses: codecov/codecov-action@v1 + name: Upload code coverage report + with: + if: success() + token: ${{ secrets.CODECOV_TOKEN }} From 15a894829fc40d78db76754ecaf94cdda0cb981c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 16:39:07 +0000 Subject: [PATCH 241/344] Fix pytests --- tests/test_bump_version.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 167c8864f7..3fe8e42643 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -80,12 +80,12 @@ def test_bump_nextflow_version(datafiles): pipeline_obj._load() # Bump the version number - nf_core.bump_version.bump_nextflow_version(pipeline_obj, "20.11.0-edge") + nf_core.bump_version.bump_nextflow_version(pipeline_obj, "21.03.0-edge") new_pipeline_obj = nf_core.utils.Pipeline(test_pipeline_dir) # Check nextflow.config new_pipeline_obj._load_pipeline_config() - assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == "!>=20.11.0-edge" + assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == "!>=21.03.0-edge" # # Check .github/workflows/ci.yml # with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: @@ -98,7 +98,7 @@ def test_bump_nextflow_version(datafiles): assert ( # "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( - "20.11.0-edge".replace("-", "--") + "21.03.0-edge".replace("-", "--") ) in readme ) From 30a7fe190328fca3ccf7bafa3017e0cd747fb56c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 16:48:17 +0000 Subject: [PATCH 242/344] Use latest Nextflow edge release for tools pipeline CI tests --- .github/workflows/create-lint-wf.yml | 8 ++++---- .github/workflows/pytest.yml | 9 --------- 2 files changed, 4 insertions(+), 13 deletions(-) diff --git a/.github/workflows/create-lint-wf.yml b/.github/workflows/create-lint-wf.yml index 08f4f4a54d..202f61594b 100644 --- a/.github/workflows/create-lint-wf.yml +++ b/.github/workflows/create-lint-wf.yml @@ -4,6 +4,8 @@ on: [push, pull_request] jobs: MakeTestWorkflow: runs-on: ubuntu-latest + env: + NXF_VER: 21.03.0-edge steps: - uses: actions/checkout@v2 name: Check out source-code repository @@ -20,12 +22,10 @@ jobs: - name: Install Nextflow env: - CAPSULE_LOG: none + CAPSULE_LOG: none run: | - mkdir /tmp/nextflow - cd /tmp/nextflow wget -qO- get.nextflow.io | bash - sudo ln -s /tmp/nextflow/nextflow /usr/local/bin/nextflow + sudo mv nextflow /usr/local/bin/ - name: Run nf-core/tools run: | diff --git a/.github/workflows/pytest.yml b/.github/workflows/pytest.yml index 83bc3213d6..f260d6974a 100644 --- a/.github/workflows/pytest.yml +++ b/.github/workflows/pytest.yml @@ -40,15 +40,6 @@ jobs: wget -qO- get.nextflow.io | bash sudo mv nextflow /usr/local/bin/ - # - name: Install Nextflow - # env: - # CAPSULE_LOG: none - # run: | - # mkdir /tmp/nextflow - # cd /tmp/nextflow - # wget -qO- get.nextflow.io | bash - # sudo ln -s /tmp/nextflow/nextflow /usr/local/bin/nextflow - - name: Test with pytest run: python3 -m pytest --color=yes --cov-report=xml --cov-config=.github/.coveragerc --cov=nf_core From 470299a23ecdd85306cd12fbec6242be884cd8be Mon Sep 17 00:00:00 2001 From: drpatelh Date: Mon, 22 Mar 2021 19:10:38 +0000 Subject: [PATCH 243/344] Add Github Actions to test a created pipeline --- .github/workflows/create-test-wf.yml | 40 ++++++++++++++++++++++++++++ 1 file changed, 40 insertions(+) create mode 100644 .github/workflows/create-test-wf.yml diff --git a/.github/workflows/create-test-wf.yml b/.github/workflows/create-test-wf.yml new file mode 100644 index 0000000000..5fd680a526 --- /dev/null +++ b/.github/workflows/create-test-wf.yml @@ -0,0 +1,40 @@ +name: Create a pipeline and test it +on: [push, pull_request] + +jobs: + RunTestWorkflow: + runs-on: ubuntu-latest + env: + NXF_VER: 21.03.0-edge + steps: + - uses: actions/checkout@v2 + name: Check out source-code repository + + - name: Set up Python 3.7 + uses: actions/setup-python@v1 + with: + python-version: 3.7 + + - name: Install python dependencies + run: | + python -m pip install --upgrade pip + pip install . + + - name: Install Nextflow + env: + CAPSULE_LOG: none + run: | + wget -qO- get.nextflow.io | bash + sudo mv nextflow /usr/local/bin/ + + - name: Run nf-core/tools + run: | + nf-core --log-file log.txt create -n testpipeline -d "This pipeline is for testing" -a "Testing McTestface" + nextflow run nf-core-testpipeline -profile test,docker + + - name: Upload log file artifact + if: ${{ always() }} + uses: actions/upload-artifact@v2 + with: + name: nf-core-log-file + path: log.txt From a42a0116c32e6a4a86f742c81a9b873ea90ca8e3 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 10:54:05 +0000 Subject: [PATCH 244/344] Fix CAPSULE indent --- .github/workflows/create-lint-wf.yml | 2 +- .github/workflows/create-test-wf.yml | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/create-lint-wf.yml b/.github/workflows/create-lint-wf.yml index 202f61594b..d676e4004f 100644 --- a/.github/workflows/create-lint-wf.yml +++ b/.github/workflows/create-lint-wf.yml @@ -22,7 +22,7 @@ jobs: - name: Install Nextflow env: - CAPSULE_LOG: none + CAPSULE_LOG: none run: | wget -qO- get.nextflow.io | bash sudo mv nextflow /usr/local/bin/ diff --git a/.github/workflows/create-test-wf.yml b/.github/workflows/create-test-wf.yml index 5fd680a526..878226c095 100644 --- a/.github/workflows/create-test-wf.yml +++ b/.github/workflows/create-test-wf.yml @@ -22,7 +22,7 @@ jobs: - name: Install Nextflow env: - CAPSULE_LOG: none + CAPSULE_LOG: none run: | wget -qO- get.nextflow.io | bash sudo mv nextflow /usr/local/bin/ From 4caa40e09c4a062cb321a530a94d0659443c269a Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 10:58:42 +0000 Subject: [PATCH 245/344] Remove duplicate file names --- nf_core/lint/files_exist.py | 2 -- 1 file changed, 2 deletions(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index baa9379564..20269cdb69 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -35,11 +35,9 @@ def files_exist(self): assets/sendmail_template.txt CHANGELOG.md CODE_OF_CONDUCT.md - CODE_OF_CONDUCT.md docs/images/nf-core-PIPELINE_logo.png docs/output.md docs/README.md - docs/README.md docs/usage.md lib/nfcore_external_java_deps.jar lib/NfcoreSchema.groovy From 8b74700e842f061be10b5e2d4342003abf73dbb8 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 11:47:57 +0000 Subject: [PATCH 246/344] Append to list of files unchanged for DSL2 --- nf_core/lint/files_unchanged.py | 3 +++ 1 file changed, 3 insertions(+) diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index 987a224288..bf22300c04 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -91,10 +91,13 @@ def files_unchanged(self): [os.path.join("assets", "email_template.txt")], [os.path.join("assets", "sendmail_template.txt")], [os.path.join("assets", f"nf-core-{short_name}_logo.png")], + [os.path.join("bin", "scrape_software_versions.py")], [os.path.join("docs", "images", f"nf-core-{short_name}_logo.png")], [os.path.join("docs", "README.md")], [os.path.join("lib", "nfcore_external_java_deps.jar")], [os.path.join("lib", "NfcoreSchema.groovy")], + [os.path.join("lib", "Checks.groovy")], + [os.path.join("lib", "Utils.groovy")], ] files_partial = [ [".gitignore", "foo"], From ea410dc7aac5ce0855f7e79165d1a80dcaaa33a7 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 11:56:21 +0000 Subject: [PATCH 247/344] Add missing file checks to linting --- nf_core/lint/files_exist.py | 53 +++++++++++++++++++++++++-------- nf_core/lint/files_unchanged.py | 44 +++++++++++++-------------- 2 files changed, 62 insertions(+), 35 deletions(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index 20269cdb69..9c9ee30cfc 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -17,6 +17,7 @@ def files_exist(self): Files that **must** be present:: .gitattributes + .gitignore .github/.dockstore.yml .github/CONTRIBUTING.md .github/ISSUE_TEMPLATE/bug_report.md @@ -33,38 +34,50 @@ def files_exist(self): assets/email_template.txt assets/nf-core-PIPELINE_logo.png assets/sendmail_template.txt + bin/scrape_software_versions.py + conf/modules.config + conf/test.config + conf/test_full.config CHANGELOG.md + CITATIONS.md CODE_OF_CONDUCT.md docs/images/nf-core-PIPELINE_logo.png docs/output.md docs/README.md docs/usage.md + lib/Checks.groovy + lib/Completion.groovy lib/nfcore_external_java_deps.jar lib/NfcoreSchema.groovy + lib/Utils.groovy + lib/Workflow.groovy + modules/local/get_software_versions.nf nextflow_schema.json nextflow.config README.md Files that *should* be present:: - 'main.nf', - 'environment.yml', - 'Dockerfile', - 'conf/base.config', - '.github/workflows/awstest.yml', - '.github/workflows/awsfulltest.yml' - + main.nf + environment.yml + Dockerfile + assets/multiqc_config.yaml + conf/base.config + conf/igenomes.config + .github/workflows/awstest.yml + .github/workflows/awsfulltest.yml + Files that *must not* be present:: - 'Singularity', - 'parameters.settings.json', - 'bin/markdown_to_html.r', - 'conf/aws.config', - '.github/workflows/push_dockerhub.yml' + Singularity + parameters.settings.json + bin/markdown_to_html.r + conf/aws.config + github/workflows/push_dockerhub.yml Files that *should not* be present:: - '.travis.yml' + .travis.yml """ passed = [] @@ -77,7 +90,9 @@ def files_exist(self): short_name = self.nf_config["manifest.name"].strip("\"'").replace("nf-core/", "") files_fail = [ [".gitattributes"], + [".gitignore"], ["CHANGELOG.md"], + ["CITATIONS.md"], ["CODE_OF_CONDUCT.md"], ["CODE_OF_CONDUCT.md"], ["LICENSE", "LICENSE.md", "LICENCE", "LICENCE.md"], # NB: British / American spelling @@ -99,19 +114,31 @@ def files_exist(self): [os.path.join("assets", "email_template.txt")], [os.path.join("assets", "sendmail_template.txt")], [os.path.join("assets", f"nf-core-{short_name}_logo.png")], + [os.path.join("bin", "scrape_software_versions.py")], + [os.path.join("conf", "modules.config")], + [os.path.join("conf", "test.config")], + [os.path.join("conf", "test_full.config")], [os.path.join("docs", "images", f"nf-core-{short_name}_logo.png")], [os.path.join("docs", "output.md")], [os.path.join("docs", "README.md")], [os.path.join("docs", "README.md")], [os.path.join("docs", "usage.md")], + [os.path.join("lib", "Checks.groovy")], + [os.path.join("lib", "Completion.groovy")], [os.path.join("lib", "nfcore_external_java_deps.jar")], [os.path.join("lib", "NfcoreSchema.groovy")], + [os.path.join("lib", "Utils.groovy")], + [os.path.join("lib", "Workflow.groovy")], + [os.path.join("modules", "local" "get_software_versions.nf")], ] + files_warn = [ ["main.nf"], ["environment.yml"], ["Dockerfile"], + [os.path.join("assets", "multiqc_config.yaml")], [os.path.join("conf", "base.config")], + [os.path.join("conf", "igenomes.config")], [os.path.join(".github", "workflows", "awstest.yml")], [os.path.join(".github", "workflows", "awsfulltest.yml")], ] diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index bf22300c04..6b9d674473 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -18,32 +18,32 @@ def files_unchanged(self): Files that must be unchanged:: - '.gitattributes', - '.github/.dockstore.yml', - '.github/CONTRIBUTING.md', - '.github/ISSUE_TEMPLATE/bug_report.md', - '.github/ISSUE_TEMPLATE/config.yml', - '.github/ISSUE_TEMPLATE/feature_request.md', - '.github/markdownlint.yml', - '.github/PULL_REQUEST_TEMPLATE.md', - '.github/workflows/branch.yml', - '.github/workflows/linting_comment.yml', - '.github/workflows/linting.yml', - 'assets/email_template.html', - 'assets/email_template.txt', - 'assets/nf-core-PIPELINE_logo.png', - 'assets/sendmail_template.txt', - 'CODE_OF_CONDUCT.md', - 'docs/images/nf-core-PIPELINE_logo.png', - 'docs/README.md', - 'lib/nfcore_external_java_deps.jar' - 'lib/NfcoreSchema.groovy', + .gitattributes + .github/.dockstore.yml + .github/CONTRIBUTING.md + .github/ISSUE_TEMPLATE/bug_report.md + .github/ISSUE_TEMPLATE/config.yml + .github/ISSUE_TEMPLATE/feature_request.md + .github/markdownlint.yml + .github/PULL_REQUEST_TEMPLATE.md + .github/workflows/branch.yml + .github/workflows/linting_comment.yml + .github/workflows/linting.yml + assets/email_template.html + assets/email_template.txt + assets/nf-core-PIPELINE_logo.png + assets/sendmail_template.txt + CODE_OF_CONDUCT.md + docs/images/nf-core-PIPELINE_logo.png + docs/README.md' + lib/nfcore_external_java_deps.jar + lib/NfcoreSchema.groovy ['LICENSE', 'LICENSE.md', 'LICENCE', 'LICENCE.md'], # NB: British / American spelling Files that can have additional content but must include the template contents:: - '.gitignore', - 'assets/multiqc_config.yaml', + .gitignore + assets/multiqc_config.yaml .. tip:: You can configure the ``nf-core lint`` tests to ignore any of these checks by setting the ``files_unchanged`` key as follows in your linting config file. For example: From 7b99d871145d28b64d2c63a121cf326c5e1c63af Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 14:04:41 +0000 Subject: [PATCH 248/344] Fix tyop --- nf_core/lint/files_exist.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index 9c9ee30cfc..cf8da57df8 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -129,7 +129,7 @@ def files_exist(self): [os.path.join("lib", "NfcoreSchema.groovy")], [os.path.join("lib", "Utils.groovy")], [os.path.join("lib", "Workflow.groovy")], - [os.path.join("modules", "local" "get_software_versions.nf")], + [os.path.join("modules", "local", "get_software_versions.nf")], ] files_warn = [ From c78626eec535cc19dfd80ebdf0d5779c7720f308 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 16:14:28 +0000 Subject: [PATCH 249/344] Bix Black tests --- nf_core/lint/files_exist.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index cf8da57df8..a9c08ab4cf 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -66,7 +66,7 @@ def files_exist(self): conf/igenomes.config .github/workflows/awstest.yml .github/workflows/awsfulltest.yml - + Files that *must not* be present:: Singularity From aa3cc18428906637f83dfb6ac835dd38f50693fa Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 17:37:56 +0000 Subject: [PATCH 250/344] Comment out licences test --- .github/workflows/create-lint-wf.yml | 4 ++-- tests/test_modules.py | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/.github/workflows/create-lint-wf.yml b/.github/workflows/create-lint-wf.yml index 569cac6599..541c81efbf 100644 --- a/.github/workflows/create-lint-wf.yml +++ b/.github/workflows/create-lint-wf.yml @@ -36,8 +36,8 @@ jobs: - name: nf-core list run: nf-core --log-file log.txt list - - name: nf-core licences - run: nf-core --log-file log.txt licences nf-core-testpipeline + # - name: nf-core licences + # run: nf-core --log-file log.txt licences nf-core-testpipeline - name: nf-core sync run: nf-core --log-file log.txt sync nf-core-testpipeline/ diff --git a/tests/test_modules.py b/tests/test_modules.py index bdbe6f2be7..b04e620e8f 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -83,7 +83,7 @@ def test_modules_lint_trimgalore(self): self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) - assert len(module_lint.passed) == 18 + assert len(module_lint.passed) == 51 assert len(module_lint.warned) == 0 assert len(module_lint.failed) == 0 From 4702560f259900dcd33f23445fce3766a9a890a2 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 23 Mar 2021 17:45:54 +0000 Subject: [PATCH 251/344] Change trimgalore warn num tests --- tests/test_modules.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index b04e620e8f..53c7cb9dbb 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -84,7 +84,7 @@ def test_modules_lint_trimgalore(self): module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) assert len(module_lint.passed) == 51 - assert len(module_lint.warned) == 0 + assert len(module_lint.warned) == 1 assert len(module_lint.failed) == 0 def test_modules_lint_empty(self): From 2f4637a854879684eee70648756d230b42c1bd22 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 6 Apr 2021 13:24:27 +0100 Subject: [PATCH 252/344] Fix colors bug --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 1 + 1 file changed, 1 insertion(+) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index a69217967a..49d19753ce 100644 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -473,6 +473,7 @@ class NfcoreSchema { * Beautify parameters for summary and return as string */ private static String paramsSummaryLog(workflow, params, json_schema) { + Map colors = Utils.logColours(params.monochrome_logs) String output = '' def params_map = paramsSummaryMap(workflow, params, json_schema) def max_chars = paramsMaxChars(params_map) From a4c07b37f0bc06780bfab263763045ca64c98fe4 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 6 Apr 2021 16:20:19 +0100 Subject: [PATCH 253/344] Use NF edge version to run linting --- nf_core/pipeline-template/.github/workflows/linting.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nf_core/pipeline-template/.github/workflows/linting.yml b/nf_core/pipeline-template/.github/workflows/linting.yml index 4570f43bda..3f92a6ab5d 100644 --- a/nf_core/pipeline-template/.github/workflows/linting.yml +++ b/nf_core/pipeline-template/.github/workflows/linting.yml @@ -87,6 +87,8 @@ jobs: nf-core: runs-on: ubuntu-latest + env: + NXF_VER: 21.03.0-edge steps: - name: Check out pipeline code From e60830422e83181539d8649c41dfdb86996ded12 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 6 Apr 2021 16:35:23 +0100 Subject: [PATCH 254/344] Change files_unchanged for DSL2 template --- nf_core/lint/files_unchanged.py | 6 ------ 1 file changed, 6 deletions(-) diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index 6b9d674473..61d1d3399a 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -24,13 +24,10 @@ def files_unchanged(self): .github/ISSUE_TEMPLATE/bug_report.md .github/ISSUE_TEMPLATE/config.yml .github/ISSUE_TEMPLATE/feature_request.md - .github/markdownlint.yml .github/PULL_REQUEST_TEMPLATE.md .github/workflows/branch.yml .github/workflows/linting_comment.yml .github/workflows/linting.yml - assets/email_template.html - assets/email_template.txt assets/nf-core-PIPELINE_logo.png assets/sendmail_template.txt CODE_OF_CONDUCT.md @@ -82,13 +79,10 @@ def files_unchanged(self): [os.path.join(".github", "ISSUE_TEMPLATE", "bug_report.md")], [os.path.join(".github", "ISSUE_TEMPLATE", "config.yml")], [os.path.join(".github", "ISSUE_TEMPLATE", "feature_request.md")], - [os.path.join(".github", "markdownlint.yml")], [os.path.join(".github", "PULL_REQUEST_TEMPLATE.md")], [os.path.join(".github", "workflows", "branch.yml")], [os.path.join(".github", "workflows", "linting_comment.yml")], [os.path.join(".github", "workflows", "linting.yml")], - [os.path.join("assets", "email_template.html")], - [os.path.join("assets", "email_template.txt")], [os.path.join("assets", "sendmail_template.txt")], [os.path.join("assets", f"nf-core-{short_name}_logo.png")], [os.path.join("bin", "scrape_software_versions.py")], From 2b6ff26ad42963468e6129cd29b0b2e29dfdf467 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 6 Apr 2021 16:51:46 +0100 Subject: [PATCH 255/344] Remove text asking to add regex to scrape_software_versions.py --- nf_core/pipeline-template/.github/PULL_REQUEST_TEMPLATE.md | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/.github/PULL_REQUEST_TEMPLATE.md b/nf_core/pipeline-template/.github/PULL_REQUEST_TEMPLATE.md index 64a0d62673..6387bb37f0 100644 --- a/nf_core/pipeline-template/.github/PULL_REQUEST_TEMPLATE.md +++ b/nf_core/pipeline-template/.github/PULL_REQUEST_TEMPLATE.md @@ -15,7 +15,6 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/{{ name }}/t - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! - - [ ] If you've added a new tool - add to the software_versions process and a regex to `scrape_software_versions.py` - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/{{ name }}/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the {{ name }} _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint .`). From 81ce2a6fa1751229fb1b44255c51d640c9c6e220 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 6 Apr 2021 17:25:03 +0100 Subject: [PATCH 256/344] Remove AWS workflow dispatch after Docker creation --- nf_core/pipeline-template/.github/workflows/awsfulltest.yml | 3 --- 1 file changed, 3 deletions(-) diff --git a/nf_core/pipeline-template/.github/workflows/awsfulltest.yml b/nf_core/pipeline-template/.github/workflows/awsfulltest.yml index 0e4bfb7ea6..236f01fa5e 100644 --- a/nf_core/pipeline-template/.github/workflows/awsfulltest.yml +++ b/nf_core/pipeline-template/.github/workflows/awsfulltest.yml @@ -4,9 +4,6 @@ name: nf-core AWS full size tests # It runs the -profile 'test_full' on AWS batch on: - workflow_run: - workflows: ["nf-core Docker push (release)"] - types: [completed] workflow_dispatch: {% raw %} From 352403589079d642e55a8cfc44a347eaf8afac5b Mon Sep 17 00:00:00 2001 From: drpatelh Date: Tue, 6 Apr 2021 17:28:06 +0100 Subject: [PATCH 257/344] Trigger AWS full tests on release --- nf_core/pipeline-template/.github/workflows/awsfulltest.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nf_core/pipeline-template/.github/workflows/awsfulltest.yml b/nf_core/pipeline-template/.github/workflows/awsfulltest.yml index 236f01fa5e..c033c7756c 100644 --- a/nf_core/pipeline-template/.github/workflows/awsfulltest.yml +++ b/nf_core/pipeline-template/.github/workflows/awsfulltest.yml @@ -4,6 +4,8 @@ name: nf-core AWS full size tests # It runs the -profile 'test_full' on AWS batch on: + release: + types: [published] workflow_dispatch: {% raw %} From 3e2cdb2377ad2d9f1ca2320ffc61f0e53bd86d6c Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 7 Apr 2021 11:53:04 +0100 Subject: [PATCH 258/344] Add in failed lint files since they can be customised with a yaml --- nf_core/lint/files_unchanged.py | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index 61d1d3399a..6b9d674473 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -24,10 +24,13 @@ def files_unchanged(self): .github/ISSUE_TEMPLATE/bug_report.md .github/ISSUE_TEMPLATE/config.yml .github/ISSUE_TEMPLATE/feature_request.md + .github/markdownlint.yml .github/PULL_REQUEST_TEMPLATE.md .github/workflows/branch.yml .github/workflows/linting_comment.yml .github/workflows/linting.yml + assets/email_template.html + assets/email_template.txt assets/nf-core-PIPELINE_logo.png assets/sendmail_template.txt CODE_OF_CONDUCT.md @@ -79,10 +82,13 @@ def files_unchanged(self): [os.path.join(".github", "ISSUE_TEMPLATE", "bug_report.md")], [os.path.join(".github", "ISSUE_TEMPLATE", "config.yml")], [os.path.join(".github", "ISSUE_TEMPLATE", "feature_request.md")], + [os.path.join(".github", "markdownlint.yml")], [os.path.join(".github", "PULL_REQUEST_TEMPLATE.md")], [os.path.join(".github", "workflows", "branch.yml")], [os.path.join(".github", "workflows", "linting_comment.yml")], [os.path.join(".github", "workflows", "linting.yml")], + [os.path.join("assets", "email_template.html")], + [os.path.join("assets", "email_template.txt")], [os.path.join("assets", "sendmail_template.txt")], [os.path.join("assets", f"nf-core-{short_name}_logo.png")], [os.path.join("bin", "scrape_software_versions.py")], From f36ceb6f41a19af8570786250750813f4cba1d33 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 7 Apr 2021 12:01:59 +0100 Subject: [PATCH 259/344] Fix AWS lint tests --- nf_core/lint/actions_awsfulltest.py | 7 +++---- 1 file changed, 3 insertions(+), 4 deletions(-) diff --git a/nf_core/lint/actions_awsfulltest.py b/nf_core/lint/actions_awsfulltest.py index 1fa931dad4..d2940af9e6 100644 --- a/nf_core/lint/actions_awsfulltest.py +++ b/nf_core/lint/actions_awsfulltest.py @@ -13,7 +13,7 @@ def actions_awsfulltest(self): The GitHub Actions workflow is called ``awsfulltest.yml``, and it can be found in the ``.github/workflows/`` directory. .. warning:: This workflow incurs AWS costs, therefore it should only be triggered for pipeline releases: - ``workflow_run`` (after the docker hub release workflow) and ``workflow_dispatch``. + ``release`` (after the pipeline release) and ``workflow_dispatch``. .. note:: You can manually trigger the AWS tests by going to the `Actions` tab on the pipeline GitHub repository and selecting the `nf-core AWS full size tests` workflow on the left. @@ -23,7 +23,7 @@ def actions_awsfulltest(self): The ``.github/workflows/awsfulltest.yml`` file is tested for the following: * Must be turned on ``workflow_dispatch``. - * Must be turned on for ``workflow_run`` with ``workflows: ["nf-core Docker push (release)"]`` and ``types: [completed]`` + * Must be turned on for ``release`` with ``types: [published]`` * Should run the profile ``test_full`` that should be edited to provide the links to full-size datasets. If it runs the profile ``test``, a warning is given. """ passed = [] @@ -43,8 +43,7 @@ def actions_awsfulltest(self): # Check that the action is only turned on for published releases try: assert "workflow_run" in wf[True] - assert wf[True]["workflow_run"]["workflows"] == ["nf-core Docker push (release)"] - assert wf[True]["workflow_run"]["types"] == ["completed"] + assert wf[True]["release"]["types"] == ["published"] assert "workflow_dispatch" in wf[True] except (AssertionError, KeyError, TypeError): failed.append("`.github/workflows/awsfulltest.yml` is not triggered correctly") From bb068c82c399fef492ebb598b4e50eabbf850cbe Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 7 Apr 2021 12:04:53 +0100 Subject: [PATCH 260/344] Use conda-name when creating TrimGalore module --- tests/test_modules.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 53c7cb9dbb..6c0eefeed5 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -100,7 +100,7 @@ def test_modules_lint_empty(self): def test_modules_create_succeed(self): """ Succeed at creating the TrimGalore! module """ module_create = nf_core.modules.ModuleCreate( - self.pipeline_dir, "trimgalore", "@author", "process_low", True, True + self.pipeline_dir, "trimgalore", "@author", "process_low", True, True, conda_name="trim-galore" ) module_create.create() assert os.path.exists(os.path.join(self.pipeline_dir, "modules", "local", "trimgalore.nf")) From 5e6853b11e121d9381a70a636d72ced49ffb63e5 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 7 Apr 2021 12:07:06 +0100 Subject: [PATCH 261/344] Fix AWS full lint test again --- nf_core/lint/actions_awsfulltest.py | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/lint/actions_awsfulltest.py b/nf_core/lint/actions_awsfulltest.py index d2940af9e6..4355ef1e8d 100644 --- a/nf_core/lint/actions_awsfulltest.py +++ b/nf_core/lint/actions_awsfulltest.py @@ -42,7 +42,6 @@ def actions_awsfulltest(self): # Check that the action is only turned on for published releases try: - assert "workflow_run" in wf[True] assert wf[True]["release"]["types"] == ["published"] assert "workflow_dispatch" in wf[True] except (AssertionError, KeyError, TypeError): From 6ec737387d0919e283d794924066ae28c63d2262 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 7 Apr 2021 12:10:30 +0100 Subject: [PATCH 262/344] Fix missing key in AWS full tests --- tests/lint/actions_awsfulltest.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/lint/actions_awsfulltest.py b/tests/lint/actions_awsfulltest.py index 767715340e..f339892393 100644 --- a/tests/lint/actions_awsfulltest.py +++ b/tests/lint/actions_awsfulltest.py @@ -47,7 +47,7 @@ def test_actions_awsfulltest_fail(self): new_pipeline = self._make_pipeline_copy() with open(os.path.join(new_pipeline, ".github", "workflows", "awsfulltest.yml"), "r") as fh: awsfulltest_yml = yaml.safe_load(fh) - del awsfulltest_yml[True]["workflow_run"] + del awsfulltest_yml[True]["release"] with open(os.path.join(new_pipeline, ".github", "workflows", "awsfulltest.yml"), "w") as fh: yaml.dump(awsfulltest_yml, fh) From 87c46d7a2fd14773fc53c9fc2615511679aca503 Mon Sep 17 00:00:00 2001 From: drpatelh Date: Wed, 7 Apr 2021 12:15:38 +0100 Subject: [PATCH 263/344] Fix last lint test failing due to Ananconda API looking for trimgalore and not trim-galore --- tests/test_modules.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 6c0eefeed5..918802bbae 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -108,7 +108,7 @@ def test_modules_create_succeed(self): def test_modules_create_fail_exists(self): """ Fail at creating the same module twice""" module_create = nf_core.modules.ModuleCreate( - self.pipeline_dir, "trimgalore", "@author", "process_low", False, False + self.pipeline_dir, "trimgalore", "@author", "process_low", False, False, conda_name="trim-galore" ) module_create.create() with 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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - image/svg+xml - - - - - - - Python package with helper tools for the nf-core community - tools - - - - - - - - - - - - - - - - - - - - - - - - - From 3df494183b04bbb907e9feeea5effa5d379210bb Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 May 2021 11:51:01 +0200 Subject: [PATCH 265/344] Add implicit declaration to the main DSL2 template --- nf_core/pipeline-template/main.nf | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index c62f7e0e18..f474d394da 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -52,11 +52,15 @@ Workflow.validateMainParams(workflow, params, json_schema, log) /* -- RUN WORKFLOW(S) -- */ //////////////////////////////////////////////////// -workflow { +workflow NFCORE_{{ short_name|upper }} { include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params ) {{ short_name|upper }} () } +workflow { + NFCORE_{{ short_name|upper }} () +} + //////////////////////////////////////////////////// /* -- THE END -- */ //////////////////////////////////////////////////// \ No newline at end of file From 45cdc1201d3fe4cd1382444b5811686bcef10cc9 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 May 2021 14:28:03 +0200 Subject: [PATCH 266/344] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9b9d3b6854..d7a336d427 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ ### Template +* Add the implicit workflow declaration to `main.nf` DSL2 template [[#1056](https://github.com/nf-core/tools/issues/1056)] * Fixed an issue regarding explicit disabling of unused container engines [[#972](https://github.com/nf-core/tools/pull/972)] * Removed trailing slash from `params.igenomes_base` to yield valid s3 paths (previous paths work with Nextflow but not aws cli) * Added a timestamp to the trace + timetime + report + dag filenames to fix overwrite issue on AWS From db95cdc7837912107c9d785925e2eb4c1e30ab34 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 13:35:31 +0100 Subject: [PATCH 267/344] Bump NF version everywhere --- .github/workflows/create-test-wf.yml | 5 +++-- nf_core/pipeline-template/.github/workflows/linting.yml | 2 -- 2 files changed, 3 insertions(+), 4 deletions(-) diff --git a/.github/workflows/create-test-wf.yml b/.github/workflows/create-test-wf.yml index 878226c095..16c2f1abf9 100644 --- a/.github/workflows/create-test-wf.yml +++ b/.github/workflows/create-test-wf.yml @@ -1,11 +1,12 @@ name: Create a pipeline and test it on: [push, pull_request] +# Uncomment if we need an edge release of Nextflow again +# env: NXF_EDGE: 1 + jobs: RunTestWorkflow: runs-on: ubuntu-latest - env: - NXF_VER: 21.03.0-edge steps: - uses: actions/checkout@v2 name: Check out source-code repository diff --git a/nf_core/pipeline-template/.github/workflows/linting.yml b/nf_core/pipeline-template/.github/workflows/linting.yml index 3f92a6ab5d..4570f43bda 100644 --- a/nf_core/pipeline-template/.github/workflows/linting.yml +++ b/nf_core/pipeline-template/.github/workflows/linting.yml @@ -87,8 +87,6 @@ jobs: nf-core: runs-on: ubuntu-latest - env: - NXF_VER: 21.03.0-edge steps: - name: Check out pipeline code From 4f9aade2c8b46282c040ee02f6750736ca9e9bdf Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 13:37:21 +0100 Subject: [PATCH 268/344] Black --- tests/test_modules.py | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index ead4936272..566af7dd72 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -142,7 +142,9 @@ def test_modules_lint_new_modules(self): def test_modules_create_succeed(self): """Succeed at creating the TrimGalore! module""" - module_create = nf_core.modules.ModuleCreate(self.pipeline_dir, "trimgalore", "@author", "process_low", True, True) + module_create = nf_core.modules.ModuleCreate( + self.pipeline_dir, "trimgalore", "@author", "process_low", True, True + ) module_create.create() assert os.path.exists(os.path.join(self.pipeline_dir, "modules", "local", "trimgalore.nf")) From 1d00d2429a58aaf067e032959864ba0230216503 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 13:44:27 +0100 Subject: [PATCH 269/344] Replace test_bump_version.py with version on master --- tests/test_bump_version.py | 51 +++++++++++++++++++------------------- 1 file changed, 25 insertions(+), 26 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 9fcb0932aa..e7c97ef106 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -28,24 +28,24 @@ def test_bump_pipeline_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - # assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" + assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" - # # Check .github/workflows/ci.yml - # with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: - # ci_yaml = yaml.safe_load(fh) - # assert ci_yaml["jobs"]["test"]["steps"][2]["run"] == "docker build --no-cache . -t nfcore/testpipeline:1.1" - # assert "docker tag nfcore/testpipeline:dev nfcore/testpipeline:1.1" in ci_yaml["jobs"]["test"]["steps"][3]["run"] + # Check .github/workflows/ci.yml + with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: + ci_yaml = yaml.safe_load(fh) + assert ci_yaml["jobs"]["test"]["steps"][2]["run"] == "docker build --no-cache . -t nfcore/testpipeline:1.1" + assert "docker tag nfcore/testpipeline:dev nfcore/testpipeline:1.1" in ci_yaml["jobs"]["test"]["steps"][3]["run"] - # # Check environment.yml - # with open(new_pipeline_obj._fp("environment.yml")) as fh: - # conda_env = yaml.safe_load(fh) - # assert conda_env["name"] == "nf-core-testpipeline-1.1" + # Check environment.yml + with open(new_pipeline_obj._fp("environment.yml")) as fh: + conda_env = yaml.safe_load(fh) + assert conda_env["name"] == "nf-core-testpipeline-1.1" - # # Check Dockerfile - # with open(new_pipeline_obj._fp("Dockerfile")) as fh: - # dockerfile = fh.read().splitlines() - # assert "ENV PATH /opt/conda/envs/nf-core-testpipeline-1.1/bin:$PATH" in dockerfile - # assert "RUN conda env export --name nf-core-testpipeline-1.1 > nf-core-testpipeline-1.1.yml" in dockerfile + # Check Dockerfile + with open(new_pipeline_obj._fp("Dockerfile")) as fh: + dockerfile = fh.read().splitlines() + assert "ENV PATH /opt/conda/envs/nf-core-testpipeline-1.1/bin:$PATH" in dockerfile + assert "RUN conda env export --name nf-core-testpipeline-1.1 > nf-core-testpipeline-1.1.yml" in dockerfile def test_dev_bump_pipeline_version(datafiles): @@ -66,7 +66,7 @@ def test_dev_bump_pipeline_version(datafiles): # Check the pipeline config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.2dev" - # assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" + assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" def test_bump_nextflow_version(datafiles): @@ -80,25 +80,24 @@ def test_bump_nextflow_version(datafiles): pipeline_obj._load() # Bump the version number - nf_core.bump_version.bump_nextflow_version(pipeline_obj, "21.03.0-edge") + nf_core.bump_version.bump_nextflow_version(pipeline_obj, "19.10.3-edge") new_pipeline_obj = nf_core.utils.Pipeline(test_pipeline_dir) # Check nextflow.config new_pipeline_obj._load_pipeline_config() - assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == "!>=21.03.0-edge" + assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == ">=19.10.3-edge" - # # Check .github/workflows/ci.yml - # with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: - # ci_yaml = yaml.safe_load(fh) - # assert ci_yaml["jobs"]["test"]["strategy"]["matrix"]["nxf_ver"][0] == "19.10.3-edge" + # Check .github/workflows/ci.yml + with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: + ci_yaml = yaml.safe_load(fh) + assert ci_yaml["jobs"]["test"]["strategy"]["matrix"]["nxf_ver"][0] == "19.10.3-edge" # Check README.md with open(new_pipeline_obj._fp("README.md")) as fh: readme = fh.read().splitlines() assert ( - # "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( - "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( - "21.03.0-edge".replace("-", "--") + "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( + "19.10.3-edge" ) in readme - ) + ) \ No newline at end of file From 44c1d0eec64335e04c66f1cf50eaf27df02330fa Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 13:57:10 +0100 Subject: [PATCH 270/344] Fix bump version tests --- tests/test_bump_version.py | 15 +-------------- 1 file changed, 1 insertion(+), 14 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index e7c97ef106..ad78e6efcf 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -28,7 +28,6 @@ def test_bump_pipeline_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:1.1" # Check .github/workflows/ci.yml with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: @@ -36,17 +35,6 @@ def test_bump_pipeline_version(datafiles): assert ci_yaml["jobs"]["test"]["steps"][2]["run"] == "docker build --no-cache . -t nfcore/testpipeline:1.1" assert "docker tag nfcore/testpipeline:dev nfcore/testpipeline:1.1" in ci_yaml["jobs"]["test"]["steps"][3]["run"] - # Check environment.yml - with open(new_pipeline_obj._fp("environment.yml")) as fh: - conda_env = yaml.safe_load(fh) - assert conda_env["name"] == "nf-core-testpipeline-1.1" - - # Check Dockerfile - with open(new_pipeline_obj._fp("Dockerfile")) as fh: - dockerfile = fh.read().splitlines() - assert "ENV PATH /opt/conda/envs/nf-core-testpipeline-1.1/bin:$PATH" in dockerfile - assert "RUN conda env export --name nf-core-testpipeline-1.1 > nf-core-testpipeline-1.1.yml" in dockerfile - def test_dev_bump_pipeline_version(datafiles): """Test that making a release works with a dev name and a leading v""" @@ -66,7 +54,6 @@ def test_dev_bump_pipeline_version(datafiles): # Check the pipeline config new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.2dev" - assert new_pipeline_obj.nf_config["process.container"].strip("'\"") == "nfcore/testpipeline:dev" def test_bump_nextflow_version(datafiles): @@ -85,7 +72,7 @@ def test_bump_nextflow_version(datafiles): # Check nextflow.config new_pipeline_obj._load_pipeline_config() - assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == ">=19.10.3-edge" + assert new_pipeline_obj.nf_config["manifest.nextflowVersion"].strip("'\"") == "!>=21.04.0" # Check .github/workflows/ci.yml with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: From cc33424425b13408aff0da0a0f790cad3ba34a7c Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 13:59:55 +0100 Subject: [PATCH 271/344] Black --- tests/test_bump_version.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index ad78e6efcf..d5ffac0e25 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -87,4 +87,4 @@ def test_bump_nextflow_version(datafiles): "19.10.3-edge" ) in readme - ) \ No newline at end of file + ) From 2dc5000b8a841fddd6806a9e0cac2dfdec6ed10a Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 15:14:18 +0100 Subject: [PATCH 272/344] Fix more tests --- tests/test_bump_version.py | 6 ------ tests/test_modules.py | 2 +- 2 files changed, 1 insertion(+), 7 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index d5ffac0e25..f8b81c1b99 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -29,12 +29,6 @@ def test_bump_pipeline_version(datafiles): new_pipeline_obj._load_pipeline_config() assert new_pipeline_obj.nf_config["manifest.version"].strip("'\"") == "1.1" - # Check .github/workflows/ci.yml - with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: - ci_yaml = yaml.safe_load(fh) - assert ci_yaml["jobs"]["test"]["steps"][2]["run"] == "docker build --no-cache . -t nfcore/testpipeline:1.1" - assert "docker tag nfcore/testpipeline:dev nfcore/testpipeline:1.1" in ci_yaml["jobs"]["test"]["steps"][3]["run"] - def test_dev_bump_pipeline_version(datafiles): """Test that making a release works with a dev name and a leading v""" diff --git a/tests/test_modules.py b/tests/test_modules.py index 566af7dd72..ba1f2d3878 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -143,7 +143,7 @@ def test_modules_lint_new_modules(self): def test_modules_create_succeed(self): """Succeed at creating the TrimGalore! module""" module_create = nf_core.modules.ModuleCreate( - self.pipeline_dir, "trimgalore", "@author", "process_low", True, True + self.pipeline_dir, "trimgalore", "@author", "process_low", True, True, conda_name="trim-galore" ) module_create.create() assert os.path.exists(os.path.join(self.pipeline_dir, "modules", "local", "trimgalore.nf")) From 348a366649c9f013d7940fb46ba6e5a96042893f Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 15:20:17 +0100 Subject: [PATCH 273/344] Change NF version everywhere in test_bump_version.py --- tests/test_bump_version.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index f8b81c1b99..1907cacbc0 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -61,7 +61,7 @@ def test_bump_nextflow_version(datafiles): pipeline_obj._load() # Bump the version number - nf_core.bump_version.bump_nextflow_version(pipeline_obj, "19.10.3-edge") + nf_core.bump_version.bump_nextflow_version(pipeline_obj, "21.04.0") new_pipeline_obj = nf_core.utils.Pipeline(test_pipeline_dir) # Check nextflow.config @@ -71,14 +71,14 @@ def test_bump_nextflow_version(datafiles): # Check .github/workflows/ci.yml with open(new_pipeline_obj._fp(".github/workflows/ci.yml")) as fh: ci_yaml = yaml.safe_load(fh) - assert ci_yaml["jobs"]["test"]["strategy"]["matrix"]["nxf_ver"][0] == "19.10.3-edge" + assert ci_yaml["jobs"]["test"]["strategy"]["matrix"]["nxf_ver"][0] == "21.04.0" # Check README.md with open(new_pipeline_obj._fp("README.md")) as fh: readme = fh.read().splitlines() assert ( "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( - "19.10.3-edge" + "21.04.0" ) in readme ) From bcf2da6b139a296bec2e0e5c61f975947e4e53ce Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 15:41:12 +0100 Subject: [PATCH 274/344] Fix NF badge in bump versions --- tests/test_bump_version.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_bump_version.py b/tests/test_bump_version.py index 1907cacbc0..bd6c5a1a57 100644 --- a/tests/test_bump_version.py +++ b/tests/test_bump_version.py @@ -77,7 +77,7 @@ def test_bump_nextflow_version(datafiles): with open(new_pipeline_obj._fp("README.md")) as fh: readme = fh.read().splitlines() assert ( - "[![Nextflow](https://img.shields.io/badge/nextflow-%E2%89%A5{}-brightgreen.svg)](https://www.nextflow.io/)".format( + "[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A5{}-23aa62.svg?labelColor=000000)](https://www.nextflow.io/)".format( "21.04.0" ) in readme From f384827a05c7e9734f0013c6541d5e5b7f39195f Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 15:49:39 +0100 Subject: [PATCH 275/344] Update strings used in pipeline template for bump versions --- nf_core/bump_version.py | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/nf_core/bump_version.py b/nf_core/bump_version.py index bdfd974907..1aa78dd097 100644 --- a/nf_core/bump_version.py +++ b/nf_core/bump_version.py @@ -132,8 +132,8 @@ def bump_nextflow_version(pipeline_obj, new_version): pipeline_obj, [ ( - r"nextflowVersion\s*=\s*[\'\"]?>={}[\'\"]?".format(current_version.replace(".", r"\.")), - "nextflowVersion = '>={}'".format(new_version), + r"nextflowVersion\s*=\s*[\'\"]?!>={}[\'\"]?".format(current_version.replace(".", r"\.")), + "nextflowVersion = '!>={}'".format(new_version), ) ], ) @@ -157,15 +157,15 @@ def bump_nextflow_version(pipeline_obj, new_version): pipeline_obj, [ ( - r"nextflow-%E2%89%A5{}-brightgreen.svg".format(current_version.replace(".", r"\.")), - "nextflow-%E2%89%A5{}-brightgreen.svg".format(new_version), + r"nextflow%20DSL2-%E2%89%A5{}-23aa62.svg".format(current_version.replace(".", r"\.")), + "nextflow%20DSL2-%E2%89%A5{}-23aa62.svg".format(new_version), ), ( - # example: 1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>=20.04.0`) - r"1\.\s*Install\s*\[`nextflow`\]\(https://nf-co\.re/usage/installation\)\s*\(`>={}`\)".format( + # example: 1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=20.04.0`) + r"1\.\s*Install\s*\[`Nextflow`\]\(https://www.nextflow.io/docs/latest/getstarted.html#installation\)\s*\(`>={}`\)".format( current_version.replace(".", r"\.") ), - "1. Install [`nextflow`](https://nf-co.re/usage/installation) (`>={}`)".format(new_version), + "1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>={}`)".format(new_version), ), ], ) From b78470060e7eaa24b762c4163b3cb8f1b12017e0 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 15:54:38 +0100 Subject: [PATCH 276/344] Change number of passed tests for test_modules_lint_trimgalore --- nf_core/bump_version.py | 66 ++--------------------------------------- tests/test_modules.py | 2 +- 2 files changed, 4 insertions(+), 64 deletions(-) diff --git a/nf_core/bump_version.py b/nf_core/bump_version.py index 1aa78dd097..759614448b 100644 --- a/nf_core/bump_version.py +++ b/nf_core/bump_version.py @@ -34,13 +34,6 @@ def bump_pipeline_version(pipeline_obj, new_version): log.info("Changing version number from '{}' to '{}'".format(current_version, new_version)) # nextflow.config - workflow manifest version - # nextflow.config - process container manifest version - docker_tag = "dev" - if new_version.replace(".", "").isdigit(): - docker_tag = new_version - else: - log.info("New version contains letters. Setting docker tag to 'dev'") - update_file_version( "nextflow.config", pipeline_obj, @@ -48,61 +41,6 @@ def bump_pipeline_version(pipeline_obj, new_version): ( r"version\s*=\s*[\'\"]?{}[\'\"]?".format(current_version.replace(".", r"\.")), "version = '{}'".format(new_version), - ), - ( - r"container\s*=\s*[\'\"]nfcore/{}:(?:{}|dev)[\'\"]".format( - pipeline_obj.pipeline_name.lower(), current_version.replace(".", r"\.") - ), - "container = 'nfcore/{}:{}'".format(pipeline_obj.pipeline_name.lower(), docker_tag), - ), - ], - ) - - # .github/workflows/ci.yml - docker build image tag - # .github/workflows/ci.yml - docker tag image - update_file_version( - os.path.join(".github", "workflows", "ci.yml"), - pipeline_obj, - [ - ( - r"docker build --no-cache . -t nfcore/{name}:(?:{tag}|dev)".format( - name=pipeline_obj.pipeline_name.lower(), tag=current_version.replace(".", r"\.") - ), - "docker build --no-cache . -t nfcore/{name}:{tag}".format( - name=pipeline_obj.pipeline_name.lower(), tag=docker_tag - ), - ), - ( - r"docker tag nfcore/{name}:dev nfcore/{name}:(?:{tag}|dev)".format( - name=pipeline_obj.pipeline_name.lower(), tag=current_version.replace(".", r"\.") - ), - "docker tag nfcore/{name}:dev nfcore/{name}:{tag}".format( - name=pipeline_obj.pipeline_name.lower(), tag=docker_tag - ), - ), - ], - ) - - # environment.yml - environment name - update_file_version( - "environment.yml", - pipeline_obj, - [ - ( - r"name: nf-core-{}-{}".format(pipeline_obj.pipeline_name.lower(), current_version.replace(".", r"\.")), - "name: nf-core-{}-{}".format(pipeline_obj.pipeline_name.lower(), new_version), - ) - ], - ) - - # Dockerfile - ENV PATH and RUN conda env create - update_file_version( - "Dockerfile", - pipeline_obj, - [ - ( - r"nf-core-{}-{}".format(pipeline_obj.pipeline_name.lower(), current_version.replace(".", r"\.")), - "nf-core-{}-{}".format(pipeline_obj.pipeline_name.lower(), new_version), ) ], ) @@ -165,7 +103,9 @@ def bump_nextflow_version(pipeline_obj, new_version): r"1\.\s*Install\s*\[`Nextflow`\]\(https://www.nextflow.io/docs/latest/getstarted.html#installation\)\s*\(`>={}`\)".format( current_version.replace(".", r"\.") ), - "1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>={}`)".format(new_version), + "1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>={}`)".format( + new_version + ), ), ], ) diff --git a/tests/test_modules.py b/tests/test_modules.py index ba1f2d3878..bc3a2b4290 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -118,7 +118,7 @@ def test_modules_lint_trimgalore(self): self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) - assert len(module_lint.passed) == 51 + assert len(module_lint.passed) == 47 assert len(module_lint.warned) == 1 assert len(module_lint.failed) == 0 From 077aec1ef265a07322dd1812b367293fbf7eafbb Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:03:51 +0100 Subject: [PATCH 277/344] Update number of warned tests for trimgalore --- tests/test_modules.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index bc3a2b4290..0c7df24f55 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -119,7 +119,7 @@ def test_modules_lint_trimgalore(self): module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, all_modules=True) assert len(module_lint.passed) == 47 - assert len(module_lint.warned) == 1 + assert len(module_lint.warned) == 8 assert len(module_lint.failed) == 0 def test_modules_lint_empty(self): From 4e48c6a0a61efe96525ea2cc8c27759d9e8b2c5d Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:24:37 +0100 Subject: [PATCH 278/344] Reinstall fastqc and multiqc modules and only check trimgalore module for tests --- .../nf-core/software/fastqc/functions.nf | 65 +++++++++++-------- .../modules/nf-core/software/fastqc/main.nf | 2 +- .../nf-core/software/multiqc/functions.nf | 65 +++++++++++-------- .../modules/nf-core/software/multiqc/main.nf | 8 +-- tests/test_modules.py | 4 +- 5 files changed, 81 insertions(+), 63 deletions(-) diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf b/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf index d25eea86b3..da9da093d3 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/functions.nf @@ -1,48 +1,57 @@ -/* - * ----------------------------------------------------- - * Utility functions used in nf-core DSL2 module files - * ----------------------------------------------------- - */ +// +// Utility functions used in nf-core DSL2 module files +// -/* - * Extract name of software tool from process name using $task.process - */ +// +// Extract name of software tool from process name using $task.process +// def getSoftwareName(task_process) { return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() } -/* - * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules - */ +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// def initOptions(Map args) { def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.publish_by_id = args.publish_by_id ?: false - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' return options } -/* - * Tidy up and join elements of a list to return a path string - */ +// +// Tidy up and join elements of a list to return a path string +// def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes return paths.join('/') } -/* - * Function to save/publish module results - */ +// +// Function to save/publish module results +// def saveFiles(Map args) { if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) + def ioptions = initOptions(args.options) def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_id) { - path_list.add(args.publish_id) + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } } if (ioptions.publish_files instanceof Map) { for (ext in ioptions.publish_files) { diff --git a/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf b/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf index 4e84787632..7ce76adffb 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/fastqc/main.nf @@ -9,7 +9,7 @@ process FASTQC { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf b/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf index d25eea86b3..da9da093d3 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/functions.nf @@ -1,48 +1,57 @@ -/* - * ----------------------------------------------------- - * Utility functions used in nf-core DSL2 module files - * ----------------------------------------------------- - */ +// +// Utility functions used in nf-core DSL2 module files +// -/* - * Extract name of software tool from process name using $task.process - */ +// +// Extract name of software tool from process name using $task.process +// def getSoftwareName(task_process) { return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() } -/* - * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules - */ +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// def initOptions(Map args) { def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.publish_by_id = args.publish_by_id ?: false - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' return options } -/* - * Tidy up and join elements of a list to return a path string - */ +// +// Tidy up and join elements of a list to return a path string +// def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes return paths.join('/') } -/* - * Function to save/publish module results - */ +// +// Function to save/publish module results +// def saveFiles(Map args) { if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) + def ioptions = initOptions(args.options) def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_id) { - path_list.add(args.publish_id) + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } } if (ioptions.publish_files instanceof Map) { for (ext in ioptions.publish_files) { diff --git a/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf b/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf index a92e533a00..da78080024 100644 --- a/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf +++ b/nf_core/pipeline-template/modules/nf-core/software/multiqc/main.nf @@ -8,13 +8,13 @@ process MULTIQC { label 'process_medium' publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename: filename, options: params.options, publish_dir: getSoftwareName(task.process), publish_id: '') } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:[:], publish_by_meta:[]) } - conda (params.enable_conda ? "bioconda::multiqc=1.9" : null) + conda (params.enable_conda ? "bioconda::multiqc=1.10.1" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container "https://depot.galaxyproject.org/singularity/multiqc:1.9--pyh9f0ad1d_0" + container "https://depot.galaxyproject.org/singularity/multiqc:1.10.1--py_0" } else { - container "quay.io/biocontainers/multiqc:1.9--pyh9f0ad1d_0" + container "quay.io/biocontainers/multiqc:1.10.1--py_0" } input: diff --git a/tests/test_modules.py b/tests/test_modules.py index 0c7df24f55..4f3e9a8d35 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -117,10 +117,10 @@ def test_modules_lint_trimgalore(self): """Test linting the TrimGalore! module""" self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) - module_lint.lint(print_results=False, all_modules=True) + module_lint.lint(print_results=False, module='trimgalore') assert len(module_lint.passed) == 47 assert len(module_lint.warned) == 8 - assert len(module_lint.failed) == 0 + assert len(module_lint.failed) == 2 def test_modules_lint_empty(self): """Test linting a pipeline with no modules installed""" From f1ea411f8d598583d47d454a7a1ce11b33520591 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:28:55 +0100 Subject: [PATCH 279/344] Update test numbers for TrimGalore --- tests/test_modules.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index 4f3e9a8d35..e588ccf350 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -118,9 +118,9 @@ def test_modules_lint_trimgalore(self): self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) module_lint.lint(print_results=False, module='trimgalore') - assert len(module_lint.passed) == 47 - assert len(module_lint.warned) == 8 - assert len(module_lint.failed) == 2 + assert len(module_lint.passed) == 19 + assert len(module_lint.warned) == 1 + assert len(module_lint.failed) == 0 def test_modules_lint_empty(self): """Test linting a pipeline with no modules installed""" From d5d815fcabbdfda1e2bb92b8cf614e51b7b3735f Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:33:13 +0100 Subject: [PATCH 280/344] Update functions.nf --- nf_core/module-template/software/functions.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/module-template/software/functions.nf b/nf_core/module-template/software/functions.nf index 4ae82de0fa..da9da093d3 100644 --- a/nf_core/module-template/software/functions.nf +++ b/nf_core/module-template/software/functions.nf @@ -1,4 +1,4 @@ -// +// // Utility functions used in nf-core DSL2 module files // From 091ef478cfeec94d5bb5e73faf208d45cc1811fb Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:36:54 +0100 Subject: [PATCH 281/344] Fix and update trimgalore module lint tests --- tests/test_modules.py | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/test_modules.py b/tests/test_modules.py index e588ccf350..88a5bc4524 100644 --- a/tests/test_modules.py +++ b/tests/test_modules.py @@ -117,9 +117,9 @@ def test_modules_lint_trimgalore(self): """Test linting the TrimGalore! module""" self.mods.install("trimgalore") module_lint = nf_core.modules.ModuleLint(dir=self.pipeline_dir) - module_lint.lint(print_results=False, module='trimgalore') - assert len(module_lint.passed) == 19 - assert len(module_lint.warned) == 1 + module_lint.lint(print_results=False, module="trimgalore") + assert len(module_lint.passed) == 20 + assert len(module_lint.warned) == 0 assert len(module_lint.failed) == 0 def test_modules_lint_empty(self): From 4e686de5005c98396d10e3bc74185794cdccd298 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:44:41 +0100 Subject: [PATCH 282/344] Remove container tests in nextflow.config --- tests/lint/nextflow_config.py | 14 -------------- 1 file changed, 14 deletions(-) diff --git a/tests/lint/nextflow_config.py b/tests/lint/nextflow_config.py index 81fcdc81fa..dbeba6030a 100644 --- a/tests/lint/nextflow_config.py +++ b/tests/lint/nextflow_config.py @@ -23,24 +23,10 @@ def test_nextflow_config_bad_name_fail(self): lint_obj._load_pipeline_config() lint_obj.nf_config["manifest.name"] = "bad_name" - lint_obj.nf_config["process.container"] = "bad_name:dev" result = lint_obj.nextflow_config() assert len(result["failed"]) == 1 assert len(result["warned"]) == 0 - -def test_nextflow_config_bad_container_name_failed(self): - """Tests that config variable existence test fails with bad container name""" - new_pipeline = self._make_pipeline_copy() - lint_obj = nf_core.lint.PipelineLint(new_pipeline) - lint_obj._load_pipeline_config() - - lint_obj.nf_config["process.container"] = "bad_name" - result = lint_obj.nextflow_config() - assert len(result["failed"]) == 0 - assert len(result["warned"]) == 1 - - def test_nextflow_config_dev_in_release_mode_failed(self): """Tests that config variable existence test fails with dev version in release mode""" new_pipeline = self._make_pipeline_copy() From 27530122e44ffbc8c136b0e2d6f29274fbecfd33 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 16:48:22 +0100 Subject: [PATCH 283/344] Remove function call to test_nextflow_config_bad_container_name_failed --- tests/lint/nextflow_config.py | 1 + tests/test_lint.py | 1 - 2 files changed, 1 insertion(+), 1 deletion(-) diff --git a/tests/lint/nextflow_config.py b/tests/lint/nextflow_config.py index dbeba6030a..4836761c04 100644 --- a/tests/lint/nextflow_config.py +++ b/tests/lint/nextflow_config.py @@ -27,6 +27,7 @@ def test_nextflow_config_bad_name_fail(self): assert len(result["failed"]) == 1 assert len(result["warned"]) == 0 + def test_nextflow_config_dev_in_release_mode_failed(self): """Tests that config variable existence test fails with dev version in release mode""" new_pipeline = self._make_pipeline_copy() diff --git a/tests/test_lint.py b/tests/test_lint.py index edc124118a..c877fd23b7 100644 --- a/tests/test_lint.py +++ b/tests/test_lint.py @@ -205,7 +205,6 @@ def test_sphinx_rst_files(self): from lint.nextflow_config import ( test_nextflow_config_example_pass, test_nextflow_config_bad_name_fail, - test_nextflow_config_bad_container_name_failed, test_nextflow_config_dev_in_release_mode_failed, ) From ac1dea4ae79aaf4542b4faa963b261c4d8fc4928 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 17:22:59 +0100 Subject: [PATCH 284/344] Print result object --- tests/lint/nextflow_config.py | 3 +++ 1 file changed, 3 insertions(+) diff --git a/tests/lint/nextflow_config.py b/tests/lint/nextflow_config.py index 4836761c04..0047c3941b 100644 --- a/tests/lint/nextflow_config.py +++ b/tests/lint/nextflow_config.py @@ -12,6 +12,7 @@ def test_nextflow_config_example_pass(self): """Tests that config variable existence test works with good pipeline example""" self.lint_obj._load_pipeline_config() result = self.lint_obj.nextflow_config() + print(result) assert len(result["failed"]) == 0 assert len(result["warned"]) == 0 @@ -24,6 +25,7 @@ def test_nextflow_config_bad_name_fail(self): lint_obj.nf_config["manifest.name"] = "bad_name" result = lint_obj.nextflow_config() + print(result) assert len(result["failed"]) == 1 assert len(result["warned"]) == 0 @@ -37,5 +39,6 @@ def test_nextflow_config_dev_in_release_mode_failed(self): lint_obj.release_mode = True lint_obj.nf_config["manifest.version"] = "dev_is_bad_name" result = lint_obj.nextflow_config() + print(result) assert len(result["failed"]) == 1 assert len(result["warned"]) == 0 From 8d975c8104a4de5c147b5608e5c048c8b2466ae9 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 17:32:10 +0100 Subject: [PATCH 285/344] Strip out lint tests for process.container in nextflow.config --- nf_core/lint/nextflow_config.py | 35 --------------------------------- 1 file changed, 35 deletions(-) diff --git a/nf_core/lint/nextflow_config.py b/nf_core/lint/nextflow_config.py index 74528d417c..b1c3f4f92e 100644 --- a/nf_core/lint/nextflow_config.py +++ b/nf_core/lint/nextflow_config.py @@ -80,12 +80,6 @@ def nextflow_config(self): * Default filenames for the timeline, trace and report * The DAG file path should end with ``.svg`` (If Graphviz is not installed, Nextflow will generate a ``.dot`` file instead) - * ``process.container`` - - * Docker Hub handle for a single default container for use by all processes. - * Must specify a tag that matches the pipeline version number if set. - * If the pipeline version number contains the string ``dev``, the DockerHub tag must be ``:dev`` - **The following variables are depreciated and fail the test if they are still present:** * ``params.version``: The old method for specifying the pipeline version. Replaced by ``manifest.version`` @@ -146,7 +140,6 @@ def nextflow_config(self): ["trace.file"], ["report.file"], ["dag.file"], - ["process.container"], ] # Old depreciated vars - fail if present config_fail_ifdefined = [ @@ -252,34 +245,6 @@ def nextflow_config(self): ).strip("\"'") ) - # Check that the process.container name is pulling the version tag or :dev - if self.nf_config.get("process.container"): - container_name = "{}:{}".format( - self.nf_config.get("manifest.name").replace("nf-core", "nfcore").strip("'"), - self.nf_config.get("manifest.version", "").strip("'"), - ) - if "dev" in self.nf_config.get("manifest.version", "") or not self.nf_config.get("manifest.version"): - container_name = "{}:dev".format( - self.nf_config.get("manifest.name").replace("nf-core", "nfcore").strip("'") - ) - try: - assert self.nf_config.get("process.container", "").strip("'") == container_name - except AssertionError: - if self.release_mode: - failed.append( - "Config ``process.container`` looks wrong. Should be ``{}`` but is ``{}``".format( - container_name, self.nf_config.get("process.container", "").strip("'") - ) - ) - else: - warned.append( - "Config ``process.container`` looks wrong. Should be ``{}`` but is ``{}``".format( - container_name, self.nf_config.get("process.container", "").strip("'") - ) - ) - else: - passed.append("Config ``process.container`` looks correct: ``{}``".format(container_name)) - # Check that the pipeline version contains ``dev`` if not self.release_mode and "manifest.version" in self.nf_config: if self.nf_config["manifest.version"].strip(" '\"").endswith("dev"): From 3c9dc8d8ac933a7c42d99ae26d5b6d0e8875dbca Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 25 May 2021 17:35:07 +0100 Subject: [PATCH 286/344] Remove print statements --- tests/lint/nextflow_config.py | 3 --- 1 file changed, 3 deletions(-) diff --git a/tests/lint/nextflow_config.py b/tests/lint/nextflow_config.py index 0047c3941b..4836761c04 100644 --- a/tests/lint/nextflow_config.py +++ b/tests/lint/nextflow_config.py @@ -12,7 +12,6 @@ def test_nextflow_config_example_pass(self): """Tests that config variable existence test works with good pipeline example""" self.lint_obj._load_pipeline_config() result = self.lint_obj.nextflow_config() - print(result) assert len(result["failed"]) == 0 assert len(result["warned"]) == 0 @@ -25,7 +24,6 @@ def test_nextflow_config_bad_name_fail(self): lint_obj.nf_config["manifest.name"] = "bad_name" result = lint_obj.nextflow_config() - print(result) assert len(result["failed"]) == 1 assert len(result["warned"]) == 0 @@ -39,6 +37,5 @@ def test_nextflow_config_dev_in_release_mode_failed(self): lint_obj.release_mode = True lint_obj.nf_config["manifest.version"] = "dev_is_bad_name" result = lint_obj.nextflow_config() - print(result) assert len(result["failed"]) == 1 assert len(result["warned"]) == 0 From 9c7914117cff7207bcf2799f6a9cea4e3ecd7362 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:27:32 +0100 Subject: [PATCH 287/344] Re-write main script --- nf_core/pipeline-template/main.nf | 87 +++++++++++++++---------------- 1 file changed, 42 insertions(+), 45 deletions(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index f474d394da..c2e3db0541 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -1,66 +1,63 @@ #!/usr/bin/env nextflow /* ======================================================================================== - {{ name }} + {{ name }} ======================================================================================== - {{ name }} Analysis Pipeline. - #### Homepage / Documentation - https://github.com/{{ name }} + Github : https://github.com/{{ name }} + Website: https://nf-co.re/{{ short_name }} + Slack : https://nfcore.slack.com/channels/{{ short_name }} ---------------------------------------------------------------------------------------- */ nextflow.enable.dsl = 2 -//////////////////////////////////////////////////// -/* -- PRINT HELP -- */ -//////////////////////////////////////////////////// - -log.info Utils.logo(workflow, params.monochrome_logs) - -def json_schema = "$projectDir/nextflow_schema.json" -if (params.help) { - // TODO nf-core: Update typical command used to run pipeline - def command = "nextflow run {{ name }} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info NfcoreSchema.paramsHelp(workflow, params, json_schema, command) - log.info Workflow.citation(workflow) - log.info Utils.dashedLine(params.monochrome_logs) - exit 0 -} - -//////////////////////////////////////////////////// -/* -- GENOME PARAMETER VALUES -- */ -//////////////////////////////////////////////////// - -params.fasta = Workflow.getGenomeAttribute(params, 'fasta') +/* +======================================================================================== + GENOME PARAMETER VALUES +======================================================================================== +*/ -//////////////////////////////////////////////////// -/* -- PRINT PARAMETER SUMMARY -- */ -//////////////////////////////////////////////////// +params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params, json_schema) -log.info NfcoreSchema.paramsSummaryLog(workflow, params, json_schema) -log.info Workflow.citation(workflow) -log.info Utils.dashedLine(params.monochrome_logs) +/* +======================================================================================== + VALIDATE & PRINT PARAMETER SUMMARY +======================================================================================== +*/ -//////////////////////////////////////////////////// -/* -- VALIDATE PARAMETERS -- */ -//////////////////////////////////////////////////// +WorkflowMain.initialise(workflow, params, log) -Workflow.validateMainParams(workflow, params, json_schema, log) +/* +======================================================================================== + NAMED WORKFLOW FOR PIPELINE +======================================================================================== +*/ -//////////////////////////////////////////////////// -/* -- RUN WORKFLOW(S) -- */ -//////////////////////////////////////////////////// +workflow NFCORE_{{ short_name|upper }} { -workflow NFCORE_{{ short_name|upper }} { - include { {{ short_name|upper }} } from './workflows/pipeline' addParams( summary_params: summary_params ) + // + // WORKFLOW: Run main {{ name }} analysis pipeline + // + include { {{ short_name|upper }} } from './workflows/pipeline' {{ short_name|upper }} () } +/* +======================================================================================== + RUN ALL WORKFLOWS +======================================================================================== +*/ + +// +// WORKFLOW: Execute a single named workflow for the pipeline +// See: https://github.com/nf-core/rnaseq/issues/619 +// workflow { - NFCORE_{{ short_name|upper }} () + NFCORE_{{ short_name|upper }} () } -//////////////////////////////////////////////////// -/* -- THE END -- */ -//////////////////////////////////////////////////// \ No newline at end of file +/* +======================================================================================== + THE END +======================================================================================== +*/ From 4f20e0cd65bcbd254f6c68e271902b1c5af1b868 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:27:45 +0100 Subject: [PATCH 288/344] Use updated functions.nf file for local modules --- .../modules/local/functions.nf | 65 +++++++++++-------- 1 file changed, 37 insertions(+), 28 deletions(-) diff --git a/nf_core/pipeline-template/modules/local/functions.nf b/nf_core/pipeline-template/modules/local/functions.nf index d25eea86b3..da9da093d3 100644 --- a/nf_core/pipeline-template/modules/local/functions.nf +++ b/nf_core/pipeline-template/modules/local/functions.nf @@ -1,48 +1,57 @@ -/* - * ----------------------------------------------------- - * Utility functions used in nf-core DSL2 module files - * ----------------------------------------------------- - */ +// +// Utility functions used in nf-core DSL2 module files +// -/* - * Extract name of software tool from process name using $task.process - */ +// +// Extract name of software tool from process name using $task.process +// def getSoftwareName(task_process) { return task_process.tokenize(':')[-1].tokenize('_')[0].toLowerCase() } -/* - * Function to initialise default values and to generate a Groovy Map of available options for nf-core modules - */ +// +// Function to initialise default values and to generate a Groovy Map of available options for nf-core modules +// def initOptions(Map args) { def Map options = [:] - options.args = args.args ?: '' - options.args2 = args.args2 ?: '' - options.publish_by_id = args.publish_by_id ?: false - options.publish_dir = args.publish_dir ?: '' - options.publish_files = args.publish_files - options.suffix = args.suffix ?: '' + options.args = args.args ?: '' + options.args2 = args.args2 ?: '' + options.args3 = args.args3 ?: '' + options.publish_by_meta = args.publish_by_meta ?: [] + options.publish_dir = args.publish_dir ?: '' + options.publish_files = args.publish_files + options.suffix = args.suffix ?: '' return options } -/* - * Tidy up and join elements of a list to return a path string - */ +// +// Tidy up and join elements of a list to return a path string +// def getPathFromList(path_list) { - def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries - paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes + def paths = path_list.findAll { item -> !item?.trim().isEmpty() } // Remove empty entries + paths = paths.collect { it.trim().replaceAll("^[/]+|[/]+\$", "") } // Trim whitespace and trailing slashes return paths.join('/') } -/* - * Function to save/publish module results - */ +// +// Function to save/publish module results +// def saveFiles(Map args) { if (!args.filename.endsWith('.version.txt')) { - def ioptions = initOptions(args.options) + def ioptions = initOptions(args.options) def path_list = [ ioptions.publish_dir ?: args.publish_dir ] - if (ioptions.publish_by_id) { - path_list.add(args.publish_id) + if (ioptions.publish_by_meta) { + def key_list = ioptions.publish_by_meta instanceof List ? ioptions.publish_by_meta : args.publish_by_meta + for (key in key_list) { + if (args.meta && key instanceof String) { + def path = key + if (args.meta.containsKey(key)) { + path = args.meta[key] instanceof Boolean ? "${key}_${args.meta[key]}".toString() : args.meta[key] + } + path = path instanceof String ? path : '' + path_list.add(path) + } + } } if (ioptions.publish_files instanceof Map) { for (ext in ioptions.publish_files) { From d2554430a457e929044086367b3c106225198d0b Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:28:04 +0100 Subject: [PATCH 289/344] Use latest module syntax for get_software_versions.nf --- .../modules/local/get_software_versions.nf | 9 +++------ 1 file changed, 3 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/modules/local/get_software_versions.nf b/nf_core/pipeline-template/modules/local/get_software_versions.nf index 06a3ff6498..a5a819487c 100644 --- a/nf_core/pipeline-template/modules/local/get_software_versions.nf +++ b/nf_core/pipeline-template/modules/local/get_software_versions.nf @@ -3,13 +3,10 @@ include { saveFiles } from './functions' params.options = [:] -/* - * Parse software version numbers - */ process GET_SOFTWARE_VERSIONS { publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -22,12 +19,12 @@ process GET_SOFTWARE_VERSIONS { input: path versions - + output: path "software_versions.csv" , emit: csv path 'software_versions_mqc.yaml', emit: yaml - script: + script: // This script is bundled with the pipeline, in {{ name }}/bin/ """ echo $workflow.manifest.version > pipeline.version.txt echo $workflow.nextflow.version > nextflow.version.txt From bfd878eed89fa684583b3c7c385f520265ffdc50 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:28:28 +0100 Subject: [PATCH 290/344] Use latest module syntax for samplesheet_check.nf --- .../modules/local/samplesheet_check.nf | 15 +++++++-------- 1 file changed, 7 insertions(+), 8 deletions(-) diff --git a/nf_core/pipeline-template/modules/local/samplesheet_check.nf b/nf_core/pipeline-template/modules/local/samplesheet_check.nf index 26f7f7df75..9bada69b98 100644 --- a/nf_core/pipeline-template/modules/local/samplesheet_check.nf +++ b/nf_core/pipeline-template/modules/local/samplesheet_check.nf @@ -3,14 +3,11 @@ include { saveFiles } from './functions' params.options = [:] -/* - * Reformat design file and check validity - */ process SAMPLESHEET_CHECK { tag "$samplesheet" publishDir "${params.outdir}", mode: params.publish_dir_mode, - saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', publish_id:'') } + saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'pipeline_info', meta:[:], publish_by_meta:[]) } conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { @@ -21,12 +18,14 @@ process SAMPLESHEET_CHECK { input: path samplesheet - + output: path '*.csv' - script: // This script is bundled with the pipeline, in {{ name }}/bin/ + script: // This script is bundled with the pipeline, in {{ name }}/bin/ """ - check_samplesheet.py $samplesheet samplesheet.valid.csv + check_samplesheet.py \\ + $samplesheet \\ + samplesheet.valid.csv """ -} \ No newline at end of file +} From 99ada529b3c156ef57bfcfc27818c300dfe9fc38 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:28:48 +0100 Subject: [PATCH 291/344] Update input_check.nf subworkflow relative to rnaseq --- .../subworkflows/local/input_check.nf | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/nf_core/pipeline-template/subworkflows/local/input_check.nf b/nf_core/pipeline-template/subworkflows/local/input_check.nf index 5eb39c502f..c743c19a05 100644 --- a/nf_core/pipeline-template/subworkflows/local/input_check.nf +++ b/nf_core/pipeline-template/subworkflows/local/input_check.nf @@ -1,6 +1,6 @@ -/* - * Check input samplesheet and get read channels - */ +// +// Check input samplesheet and get read channels +// params.options = [:] @@ -13,7 +13,7 @@ workflow INPUT_CHECK { main: SAMPLESHEET_CHECK ( samplesheet ) .splitCsv ( header:true, sep:',' ) - .map { get_sample_info(it) } + .map { create_fastq_channels(it) } .set { reads } emit: @@ -21,7 +21,7 @@ workflow INPUT_CHECK { } // Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def get_sample_info(LinkedHashMap row) { +def create_fastq_channels(LinkedHashMap row) { def meta = [:] meta.id = row.sample meta.single_end = row.single_end.toBoolean() @@ -38,5 +38,5 @@ def get_sample_info(LinkedHashMap row) { } array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] } - return array + return array } \ No newline at end of file From 0305386a9d10cc657ac3e0f61aa2953b4cd98d0d Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:29:15 +0100 Subject: [PATCH 292/344] Re-write main pipeline script --- .../pipeline-template/workflows/pipeline.nf | 125 ++++++++++-------- 1 file changed, 72 insertions(+), 53 deletions(-) diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index ad50691d24..a584c5f0ad 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -1,15 +1,13 @@ -//////////////////////////////////////////////////// -/* -- LOCAL PARAMETER VALUES -- */ -//////////////////////////////////////////////////// +/* +======================================================================================== + VALIDATE INPUTS +======================================================================================== +*/ -params.summary_params = [:] - -//////////////////////////////////////////////////// -/* -- VALIDATE INPUTS -- */ -//////////////////////////////////////////////////// +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters -Workflow.validateWorkflowParams(params, log) +Workflow{{ name[0]|upper }}{{ name[1:] }}.initialise(params, log) // TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist @@ -19,36 +17,54 @@ for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true // Check mandatory parameters if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } -//////////////////////////////////////////////////// -/* -- CONFIG FILES -- */ -//////////////////////////////////////////////////// +/* +======================================================================================== + CONFIG FILES +======================================================================================== +*/ ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() -//////////////////////////////////////////////////// -/* -- IMPORT MODULES / SUBWORKFLOWS -- */ -//////////////////////////////////////////////////// +/* +======================================================================================== + IMPORT LOCAL MODULES/SUBWORKFLOWS +======================================================================================== +*/ // Don't overwrite global params.modules, create a copy instead and use that within the main script. def modules = params.modules.clone() -def multiqc_options = modules['multiqc'] -multiqc_options.args += params.multiqc_title ? " --title \"$params.multiqc_title\"" : '' +// +// MODULE: Local to the pipeline +// +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) + +// +// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules +// +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) -// Modules: local -include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) +/* +======================================================================================== + IMPORT NF-CORE MODULES/SUBWORKFLOWS +======================================================================================== +*/ -// Modules: nf-core/modules -include { FASTQC } from '../modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from '../modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) +def multiqc_options = modules['multiqc'] +multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' -// Subworkflows: local -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) +// +// MODULE: Installed directly from nf-core/modules +// +include { FASTQC } from '../modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from '../modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) -//////////////////////////////////////////////////// -/* -- RUN MAIN WORKFLOW -- */ -//////////////////////////////////////////////////// +/* +======================================================================================== + RUN MAIN WORKFLOW +======================================================================================== +*/ // Info required for completion email and summary def multiqc_report = [] @@ -57,26 +73,24 @@ workflow {{ short_name|upper }} { ch_software_versions = Channel.empty() - /* - * SUBWORKFLOW: Read in samplesheet, validate and stage input files - */ + // + // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // INPUT_CHECK ( ch_input ) - /* - * MODULE: Run FastQC - */ + // + // MODULE: Run FastQC + // FASTQC ( INPUT_CHECK.out.reads ) ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) - - /* - * MODULE: Pipeline reporting - */ - // Get unique list of files containing version information + // + // MODULE: Pipeline reporting + // ch_software_versions .map { it -> if (it) [ it.baseName, it ] } .groupTuple() @@ -84,14 +98,15 @@ workflow {{ short_name|upper }} { .flatten() .collect() .set { ch_software_versions } - GET_SOFTWARE_VERSIONS ( - ch_software_versions + + GET_SOFTWARE_VERSIONS ( + ch_software_versions.map { it }.collect() ) - /* - * MODULE: MultiQC - */ - workflow_summary = Workflow.paramsSummaryMultiqc(workflow, params.summary_params) + // + // MODULE: MultiQC + // + workflow_summary = Workflow{{ name[0]|upper }}{{ name[1:] }}.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty() @@ -100,7 +115,7 @@ workflow {{ short_name|upper }} { ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - + MULTIQC ( ch_multiqc_files.collect() ) @@ -108,15 +123,19 @@ workflow {{ short_name|upper }} { ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) } -//////////////////////////////////////////////////// -/* -- COMPLETION EMAIL -- */ -//////////////////////////////////////////////////// +/* +======================================================================================== + COMPLETION EMAIL AND SUMMARY +======================================================================================== +*/ workflow.onComplete { - Completion.email(workflow, params, params.summary_params, projectDir, log, multiqc_report) - Completion.summary(workflow, params, log) + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + NfcoreTemplate.summary(workflow, params, log) } -//////////////////////////////////////////////////// -/* -- THE END -- */ -//////////////////////////////////////////////////// \ No newline at end of file +/* +======================================================================================== + THE END +======================================================================================== +*/ \ No newline at end of file From f5dcb9a5a2fc2db73cc987ad22db3be6c32ce47e Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 13:38:11 +0100 Subject: [PATCH 293/344] Initial commit of input samplesheet schema --- .../assets/schema_input.json | 39 +++++++++++++++++++ 1 file changed, 39 insertions(+) create mode 100644 nf_core/pipeline-template/assets/schema_input.json diff --git a/nf_core/pipeline-template/assets/schema_input.json b/nf_core/pipeline-template/assets/schema_input.json new file mode 100644 index 0000000000..c32a0275bf --- /dev/null +++ b/nf_core/pipeline-template/assets/schema_input.json @@ -0,0 +1,39 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/{{ name }}/master/schema_input.json", + "title": "{{ name }} pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "sample": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Sample name must be provided and cannot contain spaces" + }, + "fastq_1": { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + }, + "fastq_2": { + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$" + }, + { + "type": "string", + "maxLength": 0 + } + ] + } + }, + "required": [ + "sample", + "fastq_1" + ] + } +} From cedbacc02ac4521e817b74d65801c192d24ecf2e Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 14:04:23 +0100 Subject: [PATCH 294/344] Move markdownlint.yml to top-level dir --- .../{.github/markdownlint.yml => .markdownlint.yml} | 2 ++ 1 file changed, 2 insertions(+) rename nf_core/pipeline-template/{.github/markdownlint.yml => .markdownlint.yml} (90%) diff --git a/nf_core/pipeline-template/.github/markdownlint.yml b/nf_core/pipeline-template/.markdownlint.yml similarity index 90% rename from nf_core/pipeline-template/.github/markdownlint.yml rename to nf_core/pipeline-template/.markdownlint.yml index 8d7eb53b07..9e605fcfab 100644 --- a/nf_core/pipeline-template/.github/markdownlint.yml +++ b/nf_core/pipeline-template/.markdownlint.yml @@ -1,6 +1,8 @@ # Markdownlint configuration file default: true line-length: false +ul-indent: + indent: 4 no-duplicate-header: siblings_only: true no-inline-html: From b1abfb702e06c70e5ee2675f2b3e78ed5644363c Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 14:04:30 +0100 Subject: [PATCH 295/344] Fix indents --- nf_core/pipeline-template/docs/README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/docs/README.md b/nf_core/pipeline-template/docs/README.md index 4bb82007e8..6251d9c618 100644 --- a/nf_core/pipeline-template/docs/README.md +++ b/nf_core/pipeline-template/docs/README.md @@ -3,8 +3,8 @@ The {{ name }} documentation is split into the following pages: * [Usage](usage.md) - * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. + * An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. * [Output](output.md) - * An overview of the different results produced by the pipeline and how to interpret them. + * An overview of the different results produced by the pipeline and how to interpret them. You can find a lot more documentation about installing, configuring and running nf-core pipelines on the website: [https://nf-co.re](https://nf-co.re) From 7c2a42d9b6e24386a10bf30915f8b5ed03cefbcd Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 14:10:46 +0100 Subject: [PATCH 296/344] Update CITATIONS file --- nf_core/pipeline-template/CITATIONS.md | 64 +++++++++++++------------- 1 file changed, 32 insertions(+), 32 deletions(-) diff --git a/nf_core/pipeline-template/CITATIONS.md b/nf_core/pipeline-template/CITATIONS.md index 5c1031298a..e84b929891 100644 --- a/nf_core/pipeline-template/CITATIONS.md +++ b/nf_core/pipeline-template/CITATIONS.md @@ -1,32 +1,32 @@ -# {{ name }}: Citations - -## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) - -> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. - -## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) - -> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. - -## Pipeline tools - -* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - -* [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. - -## Software packaging/containerisation tools - -* [Anaconda](https://anaconda.com) - > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. - -* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) - > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. - -* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) - > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. - -* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) - -* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) - > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. +# {{ name }}: Citations + +## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) + +> Ewels PA, Peltzer A, Fillinger S, Patel H, Alneberg J, Wilm A, Garcia MU, Di Tommaso P, Nahnsen S. The nf-core framework for community-curated bioinformatics pipelines. Nat Biotechnol. 2020 Mar;38(3):276-278. doi: 10.1038/s41587-020-0439-x. PubMed PMID: 32055031. + +## [Nextflow](https://pubmed.ncbi.nlm.nih.gov/28398311/) + +> Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. + +## Pipeline tools + +* [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + +* [MultiQC](https://www.ncbi.nlm.nih.gov/pubmed/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. + +## Software packaging/containerisation tools + +* [Anaconda](https://anaconda.com) + > Anaconda Software Distribution. Computer software. Vers. 2-2.4.0. Anaconda, Nov. 2016. Web. + +* [Bioconda](https://pubmed.ncbi.nlm.nih.gov/29967506/) + > Grüning B, Dale R, Sjödin A, Chapman BA, Rowe J, Tomkins-Tinch CH, Valieris R, Köster J; Bioconda Team. Bioconda: sustainable and comprehensive software distribution for the life sciences. Nat Methods. 2018 Jul;15(7):475-476. doi: 10.1038/s41592-018-0046-7. PubMed PMID: 29967506. + +* [BioContainers](https://pubmed.ncbi.nlm.nih.gov/28379341/) + > da Veiga Leprevost F, Grüning B, Aflitos SA, Röst HL, Uszkoreit J, Barsnes H, Vaudel M, Moreno P, Gatto L, Weber J, Bai M, Jimenez RC, Sachsenberg T, Pfeuffer J, Alvarez RV, Griss J, Nesvizhskii AI, Perez-Riverol Y. BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics. 2017 Aug 15;33(16):2580-2582. doi: 10.1093/bioinformatics/btx192. PubMed PMID: 28379341; PubMed Central PMCID: PMC5870671. + +* [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + +* [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. From 087afa6e270ad0df10f971d1230fe591531bb43f Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 14:23:46 +0100 Subject: [PATCH 297/344] Update usage docs --- nf_core/pipeline-template/docs/usage.md | 154 ++++++++++++++---------- 1 file changed, 93 insertions(+), 61 deletions(-) diff --git a/nf_core/pipeline-template/docs/usage.md b/nf_core/pipeline-template/docs/usage.md index 1d0259a239..f92475dc6f 100644 --- a/nf_core/pipeline-template/docs/usage.md +++ b/nf_core/pipeline-template/docs/usage.md @@ -29,7 +29,7 @@ CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz ### Full samplesheet -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. @@ -71,7 +71,7 @@ results # Finished results (configurable, see below) # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` -## Updating the pipeline +### Updating the pipeline When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: @@ -79,9 +79,9 @@ When you run the above command, Nextflow automatically pulls the pipeline code f nextflow pull {{ name }} ``` -## Reproducibility +### Reproducibility -It's a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. +It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. First, go to the [{{ name }} releases page](https://github.com/{{ name }}/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. @@ -95,7 +95,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/). > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -107,27 +107,20 @@ They are loaded in sequence, so later profiles can overwrite earlier profiles. If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. * `docker` - * A generic configuration profile to be used with [Docker](https://docker.com/) - * Pulls software from Docker Hub: [`{{ name_docker }}`](https://hub.docker.com/r/{{ name_docker }}/) + * A generic configuration profile to be used with [Docker](https://docker.com/) * `singularity` - * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) - * Pulls software from Docker Hub: [`{{ name_docker }}`](https://hub.docker.com/r/{{ name_docker }}/) + * A generic configuration profile to be used with [Singularity](https://sylabs.io/docs/) * `podman` - * A generic configuration profile to be used with [Podman](https://podman.io/) - * Pulls software from Docker Hub: [`{{ name_docker }}`](https://hub.docker.com/r/{{ name_docker }}/) + * A generic configuration profile to be used with [Podman](https://podman.io/) * `shifter` - * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) - * Pulls software from Docker Hub: [`{{ name_docker }}`](https://hub.docker.com/r/{{ name_docker }}/) + * A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) * `charliecloud` - * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - * Pulls software from Docker Hub: [`{{ name_docker }}`](https://hub.docker.com/r/{{ name_docker }}/) + * A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) * `conda` - * Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. - * A generic configuration profile to be used with [Conda](https://conda.io/docs/) - * Pulls most software from [Bioconda](https://bioconda.github.io/) + * A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. * `test` - * A profile with a complete configuration for automated testing - * Includes links to test data so needs no other parameters + * A profile with a complete configuration for automated testing + * Includes links to test data so needs no other parameters ### `-resume` @@ -139,74 +132,113 @@ You can also supply a run name to resume a specific run: `-resume [run-name]`. U Specify the path to a specific config file (this is a core Nextflow command). See the [nf-core website documentation](https://nf-co.re/usage/configuration) for more information. -## Custom resource requests +## Custom configuration -Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with an error code of `143` (exceeded requested resources) it will automatically resubmit with higher requests (2 x original, then 3 x original). If it still fails after three times then the pipeline is stopped. +### Resource requests -Whilst these default requirements will hopefully work for most people with most data, you may find that you want to customise the compute resources that the pipeline requests. You can do this by creating a custom config file. For example, to give the workflow process `star` 32GB of memory, you could use the following config: +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. + +For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: + +```console +[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) +Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' + +Caused by: + Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) + +Command executed: + STAR \ + --genomeDir star \ + --readFilesIn WT_REP1_trimmed.fq.gz \ + --runThreadN 2 \ + --outFileNamePrefix WT_REP1. \ + + +Command exit status: + 137 + +Command output: + (empty) + +Command error: + .command.sh: line 9: 30 Killed STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. +Work dir: + /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb + +Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` +``` + +To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. ```nextflow process { - withName: star { - memory = 32.GB - } + withName: STAR_ALIGN { + memory = 100.GB + } } ``` -To find the exact name of a process you wish to modify the compute resources, check the live-status of a nextflow run displayed on your terminal or check the nextflow error for a line like so: `Error executing process > 'bwa'`. In this case the name to specify in the custom config file is `bwa`. +> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow. -See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information. +### Tool-specific options -If you are likely to be running `nf-core` pipelines regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter (see definition above). You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. +For the ultimate flexibility, we have implemented and are using Nextflow DSL2 modules in a way where it is possible for both developers and users to change tool-specific command-line arguments (e.g. providing an additional command-line argument to the `STAR_ALIGN` process) as well as publishing options (e.g. saving files produced by the `STAR_ALIGN` process that aren't saved by default by the pipeline). In the majority of instances, as a user you won't have to change the default options set by the pipeline developer(s), however, there may be edge cases where creating a simple custom config file can improve the behaviour of the pipeline if for example it is failing due to a weird error that requires setting a tool-specific parameter to deal with smaller / larger genomes. -If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). +The command-line arguments passed to STAR in the `STAR_ALIGN` module are a combination of: -## Running in the background +* Mandatory arguments or those that need to be evaluated within the scope of the module, as supplied in the [`script`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L49-L55) section of the module file. -Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. +* An [`options.args`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L56) string of non-mandatory parameters that is set to be empty by default in the module but can be overwritten when including the module in the sub-workflow / workflow context via the `addParams` Nextflow option. -The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. +The nf-core/rnaseq pipeline has a sub-workflow (see [terminology](https://github.com/nf-core/modules#terminology)) specifically to align reads with STAR and to sort, index and generate some basic stats on the resulting BAM files using SAMtools. At the top of this file we import the `STAR_ALIGN` module via the Nextflow [`include`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L10) keyword and by default the options passed to the module via the `addParams` option are set as an empty Groovy map [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/subworkflows/nf-core/align_star.nf#L5); this in turn means `options.args` will be set to empty by default in the module file too. This is an intentional design choice and allows us to implement well-written sub-workflows composed of a chain of tools that by default run with the bare minimum parameter set for any given tool in order to make it much easier to share across pipelines and to provide the flexibility for users and developers to customise any non-mandatory arguments. -Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. -Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). +When including the sub-workflow above in the main pipeline workflow we use the same `include` statement, however, we now have the ability to overwrite options for each of the tools in the sub-workflow including the [`align_options`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L225) variable that will be used specifically to overwrite the optional arguments passed to the `STAR_ALIGN` module. In this case, the options to be provided to `STAR_ALIGN` have been assigned sensible defaults by the developer(s) in the pipeline's [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L70-L74) and can be accessed and customised in the [workflow context](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/workflows/rnaseq.nf#L201-L204) too before eventually passing them to the sub-workflow as a Groovy map called `star_align_options`. These options will then be propagated from `workflow -> sub-workflow -> module`. -## Nextflow memory requirements +As mentioned at the beginning of this section it may also be necessary for users to overwrite the options passed to modules to be able to customise specific aspects of the way in which a particular tool is executed by the pipeline. Given that all of the default module options are stored in the pipeline's `modules.config` as a [`params` variable](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L24-L25) it is also possible to overwrite any of these options via a custom config file. -In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. -We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): +Say for example we want to append an additional, non-mandatory parameter (i.e. `--outFilterMismatchNmax 16`) to the arguments passed to the `STAR_ALIGN` module. Firstly, we need to copy across the default `args` specified in the [`modules.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/modules.config#L71) and create a custom config file that is a composite of the default `args` as well as the additional options you would like to provide. This is very important because Nextflow will overwrite the default value of `args` that you provide via the custom config. -```bash -NXF_OPTS='-Xms1g -Xmx4g' +As you will see in the example below, we have: + +* appended `--outFilterMismatchNmax 16` to the default `args` used by the module. +* changed the default `publish_dir` value to where the files will eventually be published in the main results directory. +* appended `'bam':''` to the default value of `publish_files` so that the BAM files generated by the process will also be saved in the top-level results directory for the module. Note: `'out':'log'` means any file/directory ending in `out` will now be saved in a separate directory called `my_star_directory/log/`. + +```nextflow +params { + modules { + 'star_align' { + args = "--quantMode TranscriptomeSAM --twopassMode Basic --outSAMtype BAM Unsorted --readFilesCommand zcat --runRNGseed 0 --outFilterMultimapNmax 20 --alignSJDBoverhangMin 1 --outSAMattributes NH HI AS NM MD --quantTranscriptomeBan Singleend --outFilterMismatchNmax 16" + publish_dir = "my_star_directory" + publish_files = ['out':'log', 'tab':'log', 'bam':''] + } + } +} ``` -## Nextflow edge releases +### nf-core/configs -Stable releases will be becoming more infrequent as Nextflow shifts its development model to becoming more dynamic via the usage of plugins. This will allow functionality to be added as an extension to the core codebase with a release cycle that could potentially be independent to that of Nextflow itself. As a result of the reduction in stable releases, some pipelines may be required to use Nextflow `edge` releases in order to be able to exploit cutting "edge" features e.g. version 3.0 of the nf-core/rnaseq pipeline requires Nextflow `>=20.11.0-edge` in order to be able to directly download Singularity containers over `http` (see [nf-core/rnaseq#496](https://github.com/nf-core/rnaseq/issues/496)). +In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. -There are a number of ways you can install Nextflow `edge` releases, the main difference with stable releases being that you have to `export` the version you would like to install before issuing the appropriate installation/execution commands as highlighted below. +See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config.html) for more information about creating your own configuration files. -* If you have Nextflow installed already, you can issue the version you would like to use on the same line as the pipeline command and it will be fetched if required before the pipeline execution. +If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -```bash -NXF_VER="20.11.0-edge" nextflow run nf-core/rnaseq -profile test,docker -r 3.0 -``` +## Running in the background -* If you have Nextflow installed already, another alternative to the option above is to `export` it as an environment variable before you run the pipeline command: +Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. -```bash -export NXF_VER="20.11.0-edge" -nextflow run nf-core/rnaseq -profile test,docker -r 3.0 -``` +The Nextflow `-bg` flag launches Nextflow in the background, detached from your terminal so that the workflow does not stop if you log out of your session. The logs are saved to a file. -* If you would like to download and install a Nextflow `edge` release from scratch with minimal fuss: +Alternatively, you can use `screen` / `tmux` or similar tool to create a detached session which you can log back into at a later time. +Some HPC setups also allow you to run nextflow within a cluster job submitted your job scheduler (from where it submits more jobs). -```bash -export NXF_VER="20.11.0-edge" -wget -qO- get.nextflow.io | bash -sudo mv nextflow /usr/local/bin/ -nextflow run nf-core/rnaseq -profile test,docker -r 3.0 -``` +## Nextflow memory requirements -> Note if you don't have `sudo` privileges required for the last command above then you can move the `nextflow` binary to somewhere else and export that directory to `$PATH` instead. One way of doing that on Linux would be to add `export PATH=$PATH:/path/to/nextflow/binary/` to your `~/.bashrc` file so that it is available every time you login to your system. +In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. +We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): -* Manually download and install Nextflow from the available [assets](https://github.com/nextflow-io/nextflow/releases) on Github. See [Nextflow installation docs](https://www.nextflow.io/docs/latest/getstarted.html#installation). +```bash +NXF_OPTS='-Xms1g -Xmx4g' +``` From f7bffd11a99159f27eddb5e20044ce9b0937c58d Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 16:11:43 +0100 Subject: [PATCH 298/344] Update output docs --- nf_core/pipeline-template/docs/output.md | 20 +++++++++----------- 1 file changed, 9 insertions(+), 11 deletions(-) diff --git a/nf_core/pipeline-template/docs/output.md b/nf_core/pipeline-template/docs/output.md index 3c57efe7c6..621733c835 100644 --- a/nf_core/pipeline-template/docs/output.md +++ b/nf_core/pipeline-template/docs/output.md @@ -22,10 +22,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d

Output files * `fastqc/` - * `*_fastqc.html`: FastQC report containing quality metrics for your untrimmed raw fastq files. - * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. - -**NB:** The FastQC plots in this directory are generated relative to the raw, input reads. They may contain adapter sequence and regions of low quality. To see how your reads look after adapter and quality trimming please refer to the FastQC reports in the `trimgalore/fastqc/` directory. + * `*_fastqc.html`: FastQC report containing quality metrics. + * `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. @@ -44,10 +42,10 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
Output files -* `multiqc/` - * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. - * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. - * `multiqc_plots/`: directory containing static images from the report in various formats. +* `multiqc/` + * `multiqc_report.html`: a standalone HTML file that can be viewed in your web browser. + * `multiqc_data/`: directory containing parsed statistics from the different tools used in the pipeline. + * `multiqc_plots/`: directory containing static images from the report in various formats.
@@ -61,9 +59,9 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ Output files * `pipeline_info/` - * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`. - * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. + * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`. + * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. From bc9a37f91c2f814ce78eeb78743bd88465a69177 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 16:14:47 +0100 Subject: [PATCH 299/344] Add notes about updated options.args to modules.config --- nf_core/pipeline-template/conf/modules.config | 36 ++++++++++--------- 1 file changed, 20 insertions(+), 16 deletions(-) diff --git a/nf_core/pipeline-template/conf/modules.config b/nf_core/pipeline-template/conf/modules.config index f26191e0f2..0b1bfdec20 100644 --- a/nf_core/pipeline-template/conf/modules.config +++ b/nf_core/pipeline-template/conf/modules.config @@ -1,20 +1,24 @@ /* - * -------------------------------------------------- - * Config file for defining DSL2 per module options - * -------------------------------------------------- - * - * Available keys to override module options: - * args = Additional arguments appended to command in module. - * args2 = Second set of arguments appended to command in module (multi-tool modules). - * publish_dir = Directory to publish results. - * publish_by_id = Publish results in separate folders by meta.id value. - * publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension - * The value of "directory" is appended to the standard "publish_dir" path as defined above. - * If publish_files == null (unspecified) - All files are published. - * If publish_files == false - No files are published. - * suffix = File name suffix for output files. - * - */ +======================================================================================== + Config file for defining DSL2 per module options +======================================================================================== + Available keys to override module options: + args = Additional arguments appended to command in module. + args2 = Second set of arguments appended to command in module (multi-tool modules). + args3 = Third set of arguments appended to command in module (multi-tool modules). + publish_dir = Directory to publish results. + publish_by_meta = Groovy list of keys available in meta map to append as directories to "publish_dir" path + If publish_by_meta = true - Value of ${meta['id']} is appended as a directory to "publish_dir" path + If publish_by_meta = ['id', 'custompath'] - If "id" is in meta map and "custompath" isn't then "${meta['id']}/custompath/" + is appended as a directory to "publish_dir" path + If publish_by_meta = false / null - No directories are appended to "publish_dir" path + publish_files = Groovy map where key = "file_ext" and value = "directory" to publish results for that file extension + The value of "directory" is appended to the standard "publish_dir" path as defined above. + If publish_files = null (unspecified) - All files are published. + If publish_files = false - No files are published. + suffix = File name suffix for output files. +---------------------------------------------------------------------------------------- +*/ params { modules { From c9767c891a2a8576cfe0e3bfe1d0f1db33573b87 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 16:27:16 +0100 Subject: [PATCH 300/344] Change comment style and formatting for base.config --- nf_core/pipeline-template/conf/base.config | 100 +++++++++++---------- 1 file changed, 51 insertions(+), 49 deletions(-) diff --git a/nf_core/pipeline-template/conf/base.config b/nf_core/pipeline-template/conf/base.config index 76d25da4cd..6b5b3d9ef4 100644 --- a/nf_core/pipeline-template/conf/base.config +++ b/nf_core/pipeline-template/conf/base.config @@ -1,55 +1,57 @@ /* - * ------------------------------------------------- - * {{ name }} Nextflow base config file - * ------------------------------------------------- - * A 'blank slate' config file, appropriate for general - * use on most high performace compute environments. - * Assumes that all software is installed and available - * on the PATH. Runs in `local` mode - all jobs will be - * run on the logged in environment. - */ +======================================================================================== + {{ name }} Nextflow base config file +======================================================================================== + A 'blank slate' config file, appropriate for general use on most high performance + compute environments. Assumes that all software is installed and available on + the PATH. Runs in `local` mode - all jobs will be run on the logged in environment. +---------------------------------------------------------------------------------------- +*/ process { - // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + // TODO nf-core: Check the defaults for all processes + cpus = { check_max( 1 * task.attempt, 'cpus' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } - maxRetries = 1 - maxErrors = '-1' + errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + maxRetries = 1 + maxErrors = '-1' - // Process-specific resource requirements - // NOTE - Please try and re-use the labels below as much as possible. - // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. - // If possible, it would be nice to keep the same label naming convention when - // adding in your local modules too. - // TODO nf-core: Customise requirements for specific processes. - // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors - withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } - } - withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } - } - withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72.GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } - } - withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } - } - withLabel:error_ignore { - errorStrategy = 'ignore' - } - withLabel:error_retry { - errorStrategy = 'retry' - maxRetries = 2 - } -} + // Process-specific resource requirements + // NOTE - Please try and re-use the labels below as much as possible. + // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. + // If possible, it would be nice to keep the same label naming convention when + // adding in your local modules too. + // TODO nf-core: Customise requirements for specific processes. + // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors + withLabel:process_low { + cpus = { check_max( 2 * task.attempt, 'cpus' ) } + memory = { check_max( 12.GB * task.attempt, 'memory' ) } + time = { check_max( 4.h * task.attempt, 'time' ) } + } + withLabel:process_medium { + cpus = { check_max( 6 * task.attempt, 'cpus' ) } + memory = { check_max( 36.GB * task.attempt, 'memory' ) } + time = { check_max( 8.h * task.attempt, 'time' ) } + } + withLabel:process_high { + cpus = { check_max( 12 * task.attempt, 'cpus' ) } + memory = { check_max( 72.GB * task.attempt, 'memory' ) } + time = { check_max( 16.h * task.attempt, 'time' ) } + } + withLabel:process_long { + time = { check_max( 20.h * task.attempt, 'time' ) } + } + withLabel:process_high_memory { + memory = { check_max( 200.GB * task.attempt, 'memory' ) } + } + withLabel:error_ignore { + errorStrategy = 'ignore' + } + withLabel:error_retry { + errorStrategy = 'retry' + maxRetries = 2 + } +} \ No newline at end of file From d19e719de3c14605965987e6c184c7fd5517cb4b Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 16:27:23 +0100 Subject: [PATCH 301/344] Change comment style and formatting for igenomes.config --- .../pipeline-template/conf/igenomes.config | 856 +++++++++--------- 1 file changed, 427 insertions(+), 429 deletions(-) diff --git a/nf_core/pipeline-template/conf/igenomes.config b/nf_core/pipeline-template/conf/igenomes.config index 8c1cf18bc6..855948def1 100644 --- a/nf_core/pipeline-template/conf/igenomes.config +++ b/nf_core/pipeline-template/conf/igenomes.config @@ -1,434 +1,432 @@ /* - * ------------------------------------------------- - * Nextflow config file for iGenomes paths - * ------------------------------------------------- - * Defines reference genomes, using iGenome paths - * Can be used by any config that customises the base - * path using $params.igenomes_base / --igenomes_base - */ +======================================================================================== + Nextflow config file for iGenomes paths +======================================================================================== + Defines reference genomes using iGenome paths. + Can be used by any config that customises the base path using: + $params.igenomes_base / --igenomes_base +---------------------------------------------------------------------------------------- +*/ params { - // illumina iGenomes reference file paths - genomes { - 'GRCh37' { - fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + // illumina iGenomes reference file paths + genomes { + 'GRCh37' { + fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/GRCh37-blacklist.bed" + } + 'GRCh38' { + fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'GRCm38' { + fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" + } + 'TAIR10' { + fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" + mito_name = "Mt" + } + 'EB2' { + fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" + } + 'UMD3.1' { + fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" + mito_name = "MT" + } + 'WBcel235' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" + mito_name = "MtDNA" + macs_gsize = "9e7" + } + 'CanFam3.1' { + fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" + mito_name = "MT" + } + 'GRCz10' { + fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'BDGP6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" + mito_name = "M" + macs_gsize = "1.2e8" + } + 'EquCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" + mito_name = "MT" + } + 'EB1' { + fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" + } + 'Galgal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Gm01' { + fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" + } + 'Mmul_1' { + fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" + mito_name = "MT" + } + 'IRGSP-1.0' { + fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'CHIMP2.1.4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" + mito_name = "MT" + } + 'Rnor_5.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'Rnor_6.0' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" + mito_name = "MT" + } + 'R64-1-1' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" + mito_name = "MT" + macs_gsize = "1.2e7" + } + 'EF2' { + fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" + mito_name = "MT" + macs_gsize = "1.21e7" + } + 'Sbi1' { + fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" + } + 'Sscrofa10.2' { + fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" + mito_name = "MT" + } + 'AGPv3' { + fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" + mito_name = "Mt" + } + 'hg38' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } + 'hg19' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "2.7e9" + blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" + } + 'mm10' { + fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.87e9" + blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" + } + 'bosTau8' { + fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'ce10' { + fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "9e7" + } + 'canFam3' { + fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" + mito_name = "chrM" + } + 'danRer10' { + fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.37e9" + } + 'dm6' { + fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" + mito_name = "chrM" + macs_gsize = "1.2e8" + } + 'equCab2' { + fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" + mito_name = "chrM" + } + 'galGal4' { + fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" + mito_name = "chrM" + } + 'panTro4' { + fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" + mito_name = "chrM" + } + 'rn6' { + fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" + mito_name = "chrM" + } + 'sacCer3' { + fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" + readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" + mito_name = "chrM" + macs_gsize = "1.2e7" + } + 'susScr3' { + fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa" + bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" + star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" + bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" + gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" + bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" + readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" + mito_name = "chrM" + } } - 'GRCh38' { - fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_5.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'hg38' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" - } - 'hg19' { - fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "2.7e9" - blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" - } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } - } -} - -plugins { - id 'nf-amazon' } From 1f703d1cc8c22597c5b5aa64d4e8fec4e525ba8e Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 16:27:30 +0100 Subject: [PATCH 302/344] Change comment style and formatting for test.config --- nf_core/pipeline-template/conf/test.config | 43 ++++++++++++---------- 1 file changed, 23 insertions(+), 20 deletions(-) diff --git a/nf_core/pipeline-template/conf/test.config b/nf_core/pipeline-template/conf/test.config index fa00e312e5..e4419fd0d1 100644 --- a/nf_core/pipeline-template/conf/test.config +++ b/nf_core/pipeline-template/conf/test.config @@ -1,26 +1,29 @@ /* - * ------------------------------------------------- - * Nextflow config file for running tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a fast and simple test. Use as follows: - * nextflow run {{ name }} -profile test, - */ +======================================================================================== + Nextflow config file for running minimal tests +======================================================================================== + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run {{ name }} -profile test, + +---------------------------------------------------------------------------------------- +*/ params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = 6.GB - max_time = 6.h + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = 6.GB + max_time = 6.h - // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - // Genome references - genome = 'R64-1-1' + // Genome references + genome = 'R64-1-1' } From b4b407c12b4e5be9150305e5c55b006cda9fc367 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 16:27:37 +0100 Subject: [PATCH 303/344] Change comment style and formatting for test_full.config --- .../pipeline-template/conf/test_full.config | 33 ++++++++++--------- 1 file changed, 18 insertions(+), 15 deletions(-) diff --git a/nf_core/pipeline-template/conf/test_full.config b/nf_core/pipeline-template/conf/test_full.config index 3abd5287f6..0fa82b7f90 100644 --- a/nf_core/pipeline-template/conf/test_full.config +++ b/nf_core/pipeline-template/conf/test_full.config @@ -1,21 +1,24 @@ /* - * ------------------------------------------------- - * Nextflow config file for running full-size tests - * ------------------------------------------------- - * Defines bundled input files and everything required - * to run a full size pipeline test. Use as follows: - * nextflow run {{ name }} -profile test_full, - */ +======================================================================================== + Nextflow config file for running full-size tests +======================================================================================== + Defines input files and everything required to run a full size pipeline test. + + Use as follows: + nextflow run {{ name }} -profile test_full, + +---------------------------------------------------------------------------------------- +*/ params { - config_profile_name = 'Full test profile' - config_profile_description = 'Full test dataset to check pipeline function' + config_profile_name = 'Full test profile' + config_profile_description = 'Full test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + // Input data for full size test + // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' - // Genome references - genome = 'R64-1-1' + // Genome references + genome = 'R64-1-1' } From 8690a69ace2d79955a88300679ad1158ec0929f9 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:27:12 +0100 Subject: [PATCH 304/344] Delete old groovy lib files and add NfcoreTemplate.groovy --- nf_core/pipeline-template/lib/Checks.groovy | 66 ----- .../pipeline-template/lib/Completion.groovy | 127 --------- .../lib/NfcoreTemplate.groovy | 266 ++++++++++++++++++ nf_core/pipeline-template/lib/Workflow.groovy | 94 ------- 4 files changed, 266 insertions(+), 287 deletions(-) delete mode 100644 nf_core/pipeline-template/lib/Checks.groovy delete mode 100644 nf_core/pipeline-template/lib/Completion.groovy create mode 100755 nf_core/pipeline-template/lib/NfcoreTemplate.groovy delete mode 100644 nf_core/pipeline-template/lib/Workflow.groovy diff --git a/nf_core/pipeline-template/lib/Checks.groovy b/nf_core/pipeline-template/lib/Checks.groovy deleted file mode 100644 index 027deda5c1..0000000000 --- a/nf_core/pipeline-template/lib/Checks.groovy +++ /dev/null @@ -1,66 +0,0 @@ -/* - * This file holds several functions used to perform standard checks for the nf-core pipeline template. - */ - -import org.yaml.snakeyaml.Yaml - -class Checks { - - static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - def required_channels = ['conda-forge', 'bioconda', 'defaults'] - def conda_check_failed = !required_channels.every { ch -> ch in channels } - - // Check that they are in the right order - conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) - conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) - - if (conda_check_failed) { - log.warn "=============================================================================\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + - " NB: The order of the channels matters!\n" + - "===================================================================================" - } - } - - static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - // related: https://github.com/nextflow-io/nextflow/issues/813 - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - // Prevent trace files to be stored on S3 since S3 does not support rolling files. - assert !params.tracedir.startsWith('s3:') : "Specify a local tracedir or run without trace! S3 cannot be used for tracefiles." - } - } - - static void hostName(workflow, params, log) { - Map colors = Utils.logColours(params.monochrome_logs) - if (params.hostnames) { - def hostname = "hostname".execute().text.trim() - params.hostnames.each { prof, hnames -> - hnames.each { hname -> - if (hostname.contains(hname) && !workflow.profile.contains(prof)) { - log.info "=${colors.yellow}====================================================${colors.reset}=\n" + - "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + - " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + - " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + - "=${colors.yellow}====================================================${colors.reset}=" - } - } - } - } - } -} diff --git a/nf_core/pipeline-template/lib/Completion.groovy b/nf_core/pipeline-template/lib/Completion.groovy deleted file mode 100644 index 968d5a47ac..0000000000 --- a/nf_core/pipeline-template/lib/Completion.groovy +++ /dev/null @@ -1,127 +0,0 @@ -/* - * Functions to be run on completion of pipeline - */ - -class Completion { - static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = workflow.manifest.version - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = Utils.logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - } - - static void summary(workflow, params, log) { - Map colors = Utils.logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - Checks.hostName(workflow, params, log) - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } -} diff --git a/nf_core/pipeline-template/lib/NfcoreTemplate.groovy b/nf_core/pipeline-template/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000000..6dd9f3f258 --- /dev/null +++ b/nf_core/pipeline-template/lib/NfcoreTemplate.groovy @@ -0,0 +1,266 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Check params.hostnames + // + public static void hostName(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (params.hostnames) { + def hostname = "hostname".execute().text.trim() + params.hostnames.each { prof, hnames -> + hnames.each { hname -> + if (hostname.contains(hname) && !workflow.profile.contains(prof)) { + log.info "=${colors.yellow}====================================================${colors.reset}=\n" + + "${colors.yellow}WARN: You are running with `-profile $workflow.profile`\n" + + " but your machine hostname is ${colors.white}'$hostname'${colors.reset}.\n" + + " ${colors.yellow_bold}Please use `-profile $prof${colors.reset}`\n" + + "=${colors.yellow}====================================================${colors.reset}=" + } + } + } + } + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = workflow.manifest.version + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + def output_hf = new File(output_d, "pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + def output_tf = new File(output_d, "pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + hostName(workflow, params, log) + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/nf_core/pipeline-template/lib/Workflow.groovy b/nf_core/pipeline-template/lib/Workflow.groovy deleted file mode 100644 index d28dcc6b3b..0000000000 --- a/nf_core/pipeline-template/lib/Workflow.groovy +++ /dev/null @@ -1,94 +0,0 @@ -/* - * This file holds several functions specific to the pipeline. - */ - -class Workflow { - - // Citation string - private static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - static void validateMainParams(workflow, params, json_schema, log) { - if (params.validate_params) { - NfcoreSchema.validateParameters(params, json_schema, log) - } - - // Check that conda channels are set-up correctly - if (params.enable_conda) { - Checks.checkCondaChannels(log) - } - - // Check AWS batch settings - Checks.awsBatch(workflow, params) - - // Check the hostnames against configured profiles - Checks.hostName(workflow, params, log) - } - - static void validateWorkflowParams(params, log) { - genomeExists(params, log) - - if (!params.fasta) { - log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - System.exit(1) - } - } - - // Exit pipeline if incorrect --genome key provided - static void genomeExists(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - log.error "=============================================================================\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "===================================================================================" - System.exit(1) - } - } - - // Get attribute from genome config file e.g. fasta - static String getGenomeAttribute(params, attribute) { - def val = '' - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - val = params.genomes[ params.genome ][ attribute ] - } - } - return val - } - - /* - * Get workflow summary for MultiQC - */ - static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } -} From 5f6b8df4ed82e8ba3d80250fb64332671643e7eb Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:27:39 +0100 Subject: [PATCH 305/344] Update NfcoreSchema.groovy --- .../pipeline-template/lib/NfcoreSchema.groovy | 449 +++++++++--------- 1 file changed, 232 insertions(+), 217 deletions(-) mode change 100644 => 100755 nf_core/pipeline-template/lib/NfcoreSchema.groovy diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy old mode 100644 new mode 100755 index fdbd375329..16986434f7 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -1,6 +1,6 @@ -/* - * This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. - */ +// +// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. +// import org.everit.json.schema.Schema import org.everit.json.schema.loader.SchemaLoader @@ -13,16 +13,23 @@ import groovy.json.JsonBuilder class NfcoreSchema { - /* - * Function to loop over all parameters defined in schema and check - * whether the given paremeters adhere to the specificiations - */ + // + // Resolve Schema path relative to main workflow directory + // + public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { + return "${workflow.projectDir}/${schema_filename}" + } + + // + // Function to loop over all parameters defined in schema and check + // whether the given parameters adhere to the specifications + // /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - private static void validateParameters(params, jsonSchema, log) { + public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { def has_error = false //=====================================================================// // Check for nextflow core params and unexpected params - def json = new File(jsonSchema).text + def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') def nf_params = [ // Options for base `nextflow` command @@ -114,8 +121,7 @@ class NfcoreSchema { def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { // Temporarily remove camelCase/camel-case params #1035 def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ @@ -126,36 +132,36 @@ class NfcoreSchema { //=====================================================================// // Validate parameters against the schema - InputStream inputStream = new File(jsonSchema).newInputStream() - JSONObject rawSchema = new JSONObject(new JSONTokener(inputStream)) + InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() + JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) // Remove anything that's in params.schema_ignore_params - rawSchema = removeIgnoredParams(rawSchema, params) + raw_schema = removeIgnoredParams(raw_schema, params) - Schema schema = SchemaLoader.load(rawSchema) + Schema schema = SchemaLoader.load(raw_schema) // Clean the parameters def cleanedParams = cleanParameters(params) // Convert to JSONObject def jsonParams = new JsonBuilder(cleanedParams) - JSONObject paramsJSON = new JSONObject(jsonParams.toString()) + JSONObject params_json = new JSONObject(jsonParams.toString()) // Validate try { - schema.validate(paramsJSON) + schema.validate(params_json) } catch (ValidationException e) { println '' log.error 'ERROR: Validation of pipeline parameters failed!' JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, paramsJSON, log) + printExceptions(exceptionJSON, params_json, log) println '' has_error = true } // Check for unexpected parameters if (unexpectedParams.size() > 0) { - Map colors = Utils.logColours(params.monochrome_logs) + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) println '' def warn_msg = 'Found unexpected parameters:' for (unexpectedParam in unexpectedParams) { @@ -171,195 +177,16 @@ class NfcoreSchema { } } - // Loop over nested exceptions and print the causingException - private static void printExceptions(exJSON, paramsJSON, log) { - def causingExceptions = exJSON['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = exJSON['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (exJSON['pointerToViolation'] == '#') { - log.error "* ${exJSON['message']}" - } - // Error with specific param - else { - def param = exJSON['pointerToViolation'] - ~/^#\// - def param_val = paramsJSON[param].toString() - log.error "* --${param}: ${exJSON['message']} (${param_val})" - } - } - for (ex in causingExceptions) { - printExceptions(ex, paramsJSON, log) - } - } - - // Remove an element from a JSONArray - private static JSONArray removeElement(jsonArray, element){ - def list = [] - int len = jsonArray.length() - for (int i=0;i - if(rawSchema.keySet().contains('definitions')){ - rawSchema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(rawSchema.keySet().contains('properties') && rawSchema.get('properties').keySet().contains(ignore_param)) { - rawSchema.get("properties").remove(ignore_param) - } - if(rawSchema.keySet().contains('required') && rawSchema.required.contains(ignore_param)) { - def cleaned_required = removeElement(rawSchema.required, ignore_param) - rawSchema.put("required", cleaned_required) - } - } - return rawSchema - } - - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - /* - * This method tries to read a JSON params file - */ - private static LinkedHashMap paramsLoad(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = paramsRead(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - /* - Method to actually read in JSON file using Groovy. - Group (as Key), values are all parameters - - Parameter1 as Key, Description as Value - - Parameter2 as Key, Description as Value - .... - Group - - - */ - private static LinkedHashMap paramsRead(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - /* - * Get maximum number of characters across all parameter names - */ - private static Integer paramsMaxChars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } - - /* - * Beautify parameters for --help - */ - private static String paramsHelp(workflow, params, json_schema, command) { - Map colors = Utils.logColours(params.monochrome_logs) + // + // Beautify parameters for --help + // + public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) Integer num_hidden = 0 String output = '' output += 'Typical pipeline command:\n\n' output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = paramsLoad(json_schema) + Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) Integer max_chars = paramsMaxChars(params_map) + 1 Integer desc_indent = max_chars + 14 Integer dec_linewidth = 160 - desc_indent @@ -403,14 +230,14 @@ class NfcoreSchema { if (num_hidden > 0){ output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset } - output += Utils.dashedLine(params.monochrome_logs) + output += NfcoreTemplate.dashedLine(params.monochrome_logs) return output } - /* - * Groovy Map summarising parameters/workflow options used by the pipeline - */ - private static LinkedHashMap paramsSummaryMap(workflow, params, json_schema) { + // + // Groovy Map summarising parameters/workflow options used by the pipeline + // + public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { // Get a selection of core Nextflow workflow options def Map workflow_summary = [:] if (workflow.revision) { @@ -433,7 +260,7 @@ class NfcoreSchema { // Get pipeline parameters defined in JSON Schema def Map params_summary = [:] def blacklist = ['hostnames'] - def params_map = paramsLoad(json_schema) + def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) for (group in params_map.keySet()) { def sub_params = new LinkedHashMap() def group_params = params_map.get(group) // This gets the parameters of that particular group @@ -476,13 +303,13 @@ class NfcoreSchema { return [ 'Core Nextflow options' : workflow_summary ] << params_summary } - /* - * Beautify parameters for summary and return as string - */ - private static String paramsSummaryLog(workflow, params, json_schema) { - Map colors = Utils.logColours(params.monochrome_logs) + // + // Beautify parameters for summary and return as string + // + public static String paramsSummaryLog(workflow, params) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) String output = '' - def params_map = paramsSummaryMap(workflow, params, json_schema) + def params_map = paramsSummaryMap(workflow, params) def max_chars = paramsMaxChars(params_map) for (group in params_map.keySet()) { def group_params = params_map.get(group) // This gets the parameters of that particular group @@ -495,7 +322,195 @@ class NfcoreSchema { } } output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" - output += Utils.dashedLine(params.monochrome_logs) + output += NfcoreTemplate.dashedLine(params.monochrome_logs) return output } + + // + // Loop over nested exceptions and print the causingException + // + private static void printExceptions(ex_json, params_json, log) { + def causingExceptions = ex_json['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (ex_json['pointerToViolation'] == '#') { + log.error "* ${ex_json['message']}" + } + // Error with specific param + else { + def param = ex_json['pointerToViolation'] - ~/^#\// + def param_val = params_json[param].toString() + log.error "* --${param}: ${ex_json['message']} (${param_val})" + } + } + for (ex in causingExceptions) { + printExceptions(ex, params_json, log) + } + } + + // + // Remove an element from a JSONArray + // + private static JSONArray removeElement(json_array, element) { + def list = [] + int len = json_array.length() + for (int i=0;i + if(raw_schema.keySet().contains('definitions')){ + raw_schema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { + raw_schema.get("properties").remove(ignore_param) + } + if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { + def cleaned_required = removeElement(raw_schema.required, ignore_param) + raw_schema.put("required", cleaned_required) + } + } + return raw_schema + } + + // + // Clean and check parameters relative to Nextflow native classes + // + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + // + // This function tries to read a JSON params file + // + private static LinkedHashMap paramsLoad(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = paramsRead(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + // + // Method to actually read in JSON file using Groovy. + // Group (as Key), values are all parameters + // - Parameter1 as Key, Description as Value + // - Parameter2 as Key, Description as Value + // .... + // Group + // - + private static LinkedHashMap paramsRead(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + // + // Get maximum number of characters across all parameter names + // + private static Integer paramsMaxChars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } } From 872c66fd3444099c10e4caaff5a292ee88793ac8 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:27:51 +0100 Subject: [PATCH 306/344] Use updated Utils.groovy --- nf_core/pipeline-template/lib/Utils.groovy | 139 +++++++-------------- 1 file changed, 47 insertions(+), 92 deletions(-) mode change 100644 => 100755 nf_core/pipeline-template/lib/Utils.groovy diff --git a/nf_core/pipeline-template/lib/Utils.groovy b/nf_core/pipeline-template/lib/Utils.groovy old mode 100644 new mode 100755 index 558f9707e0..18173e9850 --- a/nf_core/pipeline-template/lib/Utils.groovy +++ b/nf_core/pipeline-template/lib/Utils.groovy @@ -1,92 +1,47 @@ -/* - * This file holds several Groovy functions used across the nf-core pipeline template - */ - -class Utils { - private static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } - -} \ No newline at end of file +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + def required_channels = ['conda-forge', 'bioconda', 'defaults'] + def conda_check_failed = !required_channels.every { ch -> ch in channels } + + // Check that they are in the right order + conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) + conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + + if (conda_check_failed) { + log.warn "=============================================================================\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + + " NB: The order of the channels matters!\n" + + "===================================================================================" + } + } + + // + // Join module args with appropriate spacing + // + public static String joinModuleArgs(args_list) { + return ' ' + args_list.join(' ') + } +} From 011e35862f0d9309578d30de25174e83d2197817 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:28:03 +0100 Subject: [PATCH 307/344] Use actual pipeline name in script --- nf_core/pipeline-template/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index c2e3db0541..afe5199f24 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -38,7 +38,7 @@ workflow NFCORE_{{ short_name|upper }} { // // WORKFLOW: Run main {{ name }} analysis pipeline // - include { {{ short_name|upper }} } from './workflows/pipeline' + include { {{ short_name|upper }} } from './workflows/{{ short_name }}' {{ short_name|upper }} () } From 9098bb2c634c145fe2b8274225b1d20e9db3fbb8 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:28:22 +0100 Subject: [PATCH 308/344] Add WorkflowMain.groovy lib file --- .../pipeline-template/lib/WorkflowMain.groovy | 94 +++++++++++++++++++ 1 file changed, 94 insertions(+) create mode 100755 nf_core/pipeline-template/lib/WorkflowMain.groovy diff --git a/nf_core/pipeline-template/lib/WorkflowMain.groovy b/nf_core/pipeline-template/lib/WorkflowMain.groovy new file mode 100755 index 0000000000..8b790f6682 --- /dev/null +++ b/nf_core/pipeline-template/lib/WorkflowMain.groovy @@ -0,0 +1,94 @@ +// +// This file holds several functions specific to the main.nf workflow in the {{ name }} pipeline +// + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + // + // Print help to screen if required + // + public static String help(workflow, params, log) { + def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" + def help_string = '' + help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) + help_string += NfcoreSchema.paramsHelp(workflow, params, command) + help_string += '\n' + citation(workflow) + '\n' + help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) + return help_string + } + + // + // Print parameter summary log to screen + // + public static String paramsSummaryLog(workflow, params, log) { + def summary_log = '' + summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) + summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) + summary_log += '\n' + citation(workflow) + '\n' + summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) + return summary_log + } + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log) { + // Print help to screen if required + if (params.help) { + log.info help(workflow, params, log) + System.exit(0) + } + + // Validate workflow parameters via the JSON schema + if (params.validate_params) { + NfcoreSchema.validateParameters(workflow, params, log) + } + + // Print parameter summary log to screen + log.info paramsSummaryLog(workflow, params, log) + + // Check that conda channels are set-up correctly + if (params.enable_conda) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check the hostnames against configured profiles + NfcoreTemplate.hostName(workflow, params, log) + + // Check input has been provided + if (!params.input) { + log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" + System.exit(1) + } + } + + // + // Get attribute from genome config file e.g. fasta + // + public static String getGenomeAttribute(params, attribute) { + def val = '' + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + val = params.genomes[ params.genome ][ attribute ] + } + } + return val + } +} From 73058bf377bb509fbbdc58b1512fd1aad22fb343 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:28:33 +0100 Subject: [PATCH 309/344] Add WorkflowPipeline.groovy lib file --- .../lib/WorkflowPipeline.groovy | 59 +++++++++++++++++++ 1 file changed, 59 insertions(+) create mode 100755 nf_core/pipeline-template/lib/WorkflowPipeline.groovy diff --git a/nf_core/pipeline-template/lib/WorkflowPipeline.groovy b/nf_core/pipeline-template/lib/WorkflowPipeline.groovy new file mode 100755 index 0000000000..b5bf141b3a --- /dev/null +++ b/nf_core/pipeline-template/lib/WorkflowPipeline.groovy @@ -0,0 +1,59 @@ +// +// This file holds several functions specific to the workflow/{{ short_name }}.nf in the {{ name }} pipeline +// + +class Workflow{{ name[0]|upper }}{{ name[1:] }} { + + // + // Check and validate parameters + // + public static void initialise(params, log, valid_params) { + genomeExistsError(params, log) + + if (!params.fasta) { + log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + System.exit(1) + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

$group

\n" + summary_section += "
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "
\n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + log.error "=============================================================================\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "===================================================================================" + System.exit(1) + } + } +} From 375825d3641661cfa08fbffcd8349cf8f8b13824 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 17:43:10 +0100 Subject: [PATCH 310/344] Remove container version for DSL2 from bug_report.md --- nf_core/pipeline-template/.github/ISSUE_TEMPLATE/bug_report.md | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/.github/ISSUE_TEMPLATE/bug_report.md b/nf_core/pipeline-template/.github/ISSUE_TEMPLATE/bug_report.md index 391d20eb79..5c5100c97b 100644 --- a/nf_core/pipeline-template/.github/ISSUE_TEMPLATE/bug_report.md +++ b/nf_core/pipeline-template/.github/ISSUE_TEMPLATE/bug_report.md @@ -51,13 +51,12 @@ Have you provided the following extra information/files: ## Nextflow Installation -- Version: +- Version: ## Container engine - Engine: - version: -- Image tag: ## Additional context From ba064d63a0a97b2f82f9d6a1c7e352fa089896c4 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 20:14:13 +0100 Subject: [PATCH 311/344] Fix indents in nextflow.config --- nf_core/pipeline-template/nextflow.config | 195 +++++++++++----------- 1 file changed, 98 insertions(+), 97 deletions(-) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index d3be2bd916..4c3428f036 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -1,56 +1,57 @@ /* - * ------------------------------------------------- - * {{ name }} Nextflow config file - * ------------------------------------------------- - * Default config options for all environments. - */ +======================================================================================== + {{ name }} Nextflow config file +======================================================================================== + Default config options for all compute environments +---------------------------------------------------------------------------------------- +*/ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags - // Input options - input = '' - - // References - genome = '' - igenomes_base = 's3://ngi-igenomes/igenomes/' - igenomes_ignore = false - - // MultiQC options - multiqc_config = '' - multiqc_title = '' - max_multiqc_email_size = 25.MB - - // Boilerplate options - outdir = './results' - tracedir = "${params.outdir}/pipeline_info" - publish_dir_mode = 'copy' - email = '' - email_on_fail = '' - plaintext_email = false - monochrome_logs = false - help = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes,modules' - enable_conda = false - singularity_pull_docker_container = false - - // Config options - custom_config_version = 'master' - custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - hostnames = [:] - config_profile_description = '' - config_profile_contact = '' - config_profile_url = '' - config_profile_name = '' - - // Max resource options - // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' + // TODO nf-core: Specify your pipeline's command line flags + // Input options + input = null + + // References + genome = null + igenomes_base = 's3://ngi-igenomes/igenomes/' + igenomes_ignore = false + + // MultiQC options + multiqc_config = null + multiqc_title = null + max_multiqc_email_size = '25.MB' + + // Boilerplate options + outdir = './results' + tracedir = "${params.outdir}/pipeline_info" + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + help = false + validate_params = true + show_hidden_params = false + schema_ignore_params = 'genomes,modules' + enable_conda = false + singularity_pull_docker_container = false + + // Config options + custom_config_version = 'master' + custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" + hostnames = [:] + config_profile_description = null + config_profile_contact = null + config_profile_url = null + config_profile_name = null + + // Max resource options + // Defaults only, expecting to be overwritten + max_memory = '128.GB' + max_cpus = 16 + max_time = '240.h' } @@ -62,16 +63,16 @@ includeConfig 'conf/modules.config' // Load nf-core custom profiles from different Institutions try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" + includeConfig "${params.custom_config_base}/nfcore_custom.config" } catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") + System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } // Load igenomes.config if required if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' + includeConfig 'conf/igenomes.config' } else { - params.genomes = [:] + params.genomes = [:] } profiles { @@ -132,9 +133,9 @@ profiles { // Export these variables to prevent local Python/R libraries from conflicting with those in the container env { - PYTHONNOUSERSITE = 1 - R_PROFILE_USER = "/.Rprofile" - R_ENVIRON_USER = "/.Renviron" + PYTHONNOUSERSITE = 1 + R_PROFILE_USER = "/.Rprofile" + R_ENVIRON_USER = "/.Renviron" } // Capture exit codes from upstream processes when piping @@ -142,61 +143,61 @@ process.shell = ['/bin/bash', '-euo', 'pipefail'] def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { - enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" + enabled = true + file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" } report { - enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" + enabled = true + file = "${params.tracedir}/execution_report_${trace_timestamp}.html" } trace { - enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" + enabled = true + file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" } dag { - enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" + enabled = true + file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.svg" } manifest { - name = '{{ name }}' - author = '{{ author }}' - homePage = 'https://github.com/{{ name }}' - description = '{{ description }}' - mainScript = 'main.nf' - nextflowVersion = '!>=21.04.0' - version = '{{ version }}' + name = '{{ name }}' + author = '{{ author }}' + homePage = 'https://github.com/{{ name }}' + description = '{{ description }}' + mainScript = 'main.nf' + nextflowVersion = '!>=21.04.0' + version = '{{ version }}' } // Function to ensure that resource requirements don't go beyond // a maximum limit def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj + if (type == 'memory') { + try { + if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) + return params.max_memory as nextflow.util.MemoryUnit + else + return obj + } catch (all) { + println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'time') { + try { + if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) + return params.max_time as nextflow.util.Duration + else + return obj + } catch (all) { + println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" + return obj + } + } else if (type == 'cpus') { + try { + return Math.min( obj, params.max_cpus as int ) + } catch (all) { + println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" + return obj + } } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } - } } From 20a0c7d9dda13f82185fe8b95c472227566bd856 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 20:25:15 +0100 Subject: [PATCH 312/344] Update nextflow_schema.json --- .../pipeline-template/nextflow_schema.json | 266 +++++++++--------- 1 file changed, 133 insertions(+), 133 deletions(-) diff --git a/nf_core/pipeline-template/nextflow_schema.json b/nf_core/pipeline-template/nextflow_schema.json index 90e07e1607..806170e7b1 100644 --- a/nf_core/pipeline-template/nextflow_schema.json +++ b/nf_core/pipeline-template/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/{{ name }}/master/nextflow_schema.json", "title": "{{ name }} pipeline parameters", - "description": "{{ description }}", + "description": "Nextflow RNA-Seq analysis pipeline, part of the nf-core community.", "type": "object", "definitions": { "input_output_options": { @@ -10,14 +10,15 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input" - ], "properties": { "input": { "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "pattern": "\\.csv$", + "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/{{ short_name }}/usage#samplesheet-input).", "fa_icon": "fas fa-file-csv" }, "outdir": { @@ -32,6 +33,11 @@ "fa_icon": "fas fa-envelope", "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" } } }, @@ -45,16 +51,20 @@ "type": "string", "description": "Name of iGenomes reference.", "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`.\n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." }, "fasta": { "type": "string", - "fa_icon": "fas fa-file-code", + "format": "file-path", + "mimetype": "text/plain", + "pattern": "\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified." + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" }, "igenomes_base": { "type": "string", + "format": "directory-path", "description": "Directory / URL base for iGenomes references.", "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", @@ -69,110 +79,57 @@ } } }, - "generic_options": { - "title": "Generic options", + "institutional_config_options": { + "title": "Institutional config options", "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "hidden": true, - "fa_icon": "fas fa-question-circle" - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature", - "hidden": true - }, - "max_multiqc_email_size": { + "custom_config_version": { "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true, - "help_text": "If file generated by pipeline exceeds the threshold, it will not be attached." - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", + "description": "Git commit id for Institutional configs.", + "default": "master", "hidden": true, - "help_text": "Set to receive plain-text e-mails instead of HTML formatted." + "fa_icon": "fas fa-users-cog" }, - "email_on_fail": { + "custom_config_base": { "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", "hidden": true, - "help_text": "This works exactly as with `--email`, except emails are only sent if the workflow is not successful." + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" }, - "publish_dir_mode": { + "hostnames": { "type": "string", - "default": "copy", + "description": "Institutional configs hostname.", "hidden": true, - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ] + "fa_icon": "fas fa-users-cog" }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", "hidden": true, - "help_text": "Set to disable colourful command line output and live life in monochrome." + "fa_icon": "fas fa-users-cog" }, - "tracedir": { + "config_profile_description": { "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", + "description": "Institutional config description.", "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + "fa_icon": "fas fa-users-cog" }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", "hidden": true, - "fa_icon": "fas fa-bacon" + "fa_icon": "fas fa-users-cog" }, - "singularity_pull_docker_container": { - "type": "boolean", - "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", "hidden": true, - "fa_icon": "fas fa-toolbox", - "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." + "fa_icon": "fas fa-users-cog" } } }, @@ -185,7 +142,7 @@ "properties": { "max_cpus": { "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", + "description": "Maximum number of CPUs that can be requested for any single job.", "default": 16, "fa_icon": "fas fa-microchip", "hidden": true, @@ -211,58 +168,101 @@ } } }, - "institutional_config_options": { - "title": "Institutional config options", + "generic_options": { + "title": "Generic options", "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", "properties": { - "custom_config_version": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog", - "help_text": "Provide git commit id for custom Institutional configs hosted at `nf-core/configs`. This was implemented for reproducibility purposes. Default: `master`.\n\n```bash\n## Download and use config file with following git commit id\n--custom_config_version d52db660777c4bf36546ddb188ec530c3ada1b96\n```" + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true }, - "custom_config_base": { + "email_on_fail": { "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell nextflow where to find them with the `custom_config_base` option. For example:\n\n```bash\n## Download and unzip the config files\ncd /path/to/my/configs\nwget https://github.com/nf-core/configs/archive/master.zip\nunzip master.zip\n\n## Run the pipeline\ncd /path/to/my/data\nnextflow run /path/to/pipeline/ --custom_config_base /path/to/my/configs/configs-master/\n```\n\n> Note that the nf-core/tools helper package has a `download` command to download all required pipeline files + singularity containers + institutional configs in one go for you, to make this process easier.", - "fa_icon": "fas fa-users-cog" + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true }, - "hostnames": { + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { "type": "string", - "description": "Institutional configs hostname.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "File size limit when attaching MultiQC reports to summary emails.", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true }, - "config_profile_name": { + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "multiqc_config": { "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true }, - "config_profile_description": { + "tracedir": { "type": "string", - "description": "Institutional config description.", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", "hidden": true, - "fa_icon": "fas fa-users-cog" + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", "hidden": true, - "fa_icon": "fas fa-users-cog" + "fa_icon": "fas fa-bacon" }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead.", "hidden": true, - "fa_icon": "fas fa-users-cog" + "fa_icon": "fas fa-toolbox", + "help_text": "This may be useful for example if you are unable to directly pull Singularity containers to run the pipeline due to http/https proxy issues." } } } @@ -275,13 +275,13 @@ "$ref": "#/definitions/reference_genome_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/definitions/institutional_config_options" }, { "$ref": "#/definitions/max_job_request_options" }, { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/definitions/generic_options" } ] -} +} \ No newline at end of file From 2eff6658638e6ad713dee074b3ca86861ec1c525 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 20:46:47 +0100 Subject: [PATCH 313/344] Update README.md --- nf_core/pipeline-template/README.md | 17 ++++++++--------- 1 file changed, 8 insertions(+), 9 deletions(-) diff --git a/nf_core/pipeline-template/README.md b/nf_core/pipeline-template/README.md index ae99b0db16..135534ba6b 100644 --- a/nf_core/pipeline-template/README.md +++ b/nf_core/pipeline-template/README.md @@ -19,7 +19,7 @@ **{{ name }}** is a bioinformatics best-practice analysis pipeline for {{ description }}. -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker / Singularity containers making installation trivial and results highly reproducible. +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/{{ short_name }}/results). @@ -39,23 +39,23 @@ On release, automated continuous integration tests run the pipeline on a full-si 3. Download the pipeline and test it on a minimal dataset with a single command: - ```bash + ```console nextflow run {{ name }} -profile test, ``` - * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. - * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. It is also highly recommended to use the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) settings to store the images in a central location for future pipeline runs. - * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. + > * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. + > * If you are using `singularity` then the pipeline will auto-detect this and attempt to download the Singularity images directly as opposed to performing a conversion from Docker images. If you are persistently observing issues downloading Singularity images directly due to timeout or network issues then please use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, it is highly recommended to use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to pre-download all of the required containers before running the pipeline and to set the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options to be able to store and re-use the images from a central location for future pipeline runs. + > * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. 4. Start running your own analysis! - ```bash + ```console nextflow run {{ name }} -profile --input samplesheet.csv --genome GRCh37 ``` -See [usage docs](https://nf-co.re/{{ short_name }}/usage) for all of the available options when running the pipeline. +See [usage](https://nf-co.re/rnaseq/usage) and [parameter](https://nf-co.re/rnaseq/parameters) docs for all of the available options when running the pipeline. ## Documentation @@ -65,8 +65,7 @@ The {{ name }} pipeline comes with documentation about the pipeline: [usage](htt {{ name }} was originally written by {{ author }}. -We thank the following people for their extensive assistance in the development -of this pipeline: +We thank the following people for their extensive assistance in the development of this pipeline: From e45185961ef6b77a1225f3c38feefc0d33c4c1ce Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 20:47:12 +0100 Subject: [PATCH 314/344] Fix indents in sendmail_template.txt --- .../assets/sendmail_template.txt | 36 +++++++++---------- 1 file changed, 18 insertions(+), 18 deletions(-) diff --git a/nf_core/pipeline-template/assets/sendmail_template.txt b/nf_core/pipeline-template/assets/sendmail_template.txt index a415ceedf8..1abf5b0ab5 100644 --- a/nf_core/pipeline-template/assets/sendmail_template.txt +++ b/nf_core/pipeline-template/assets/sendmail_template.txt @@ -15,15 +15,15 @@ Content-ID: Content-Disposition: inline; filename="{{ name_noslash }}_logo.png" <% out << new File("$projectDir/assets/{{ name_noslash }}_logo.png"). - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' ) %> + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> <% if (mqcFile){ @@ -37,15 +37,15 @@ Content-ID: Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" ${mqcFileObj. - bytes. - encodeBase64(). - toString(). - tokenize( '\n' )*. - toList()*. - collate( 76 )*. - collect { it.join() }. - flatten(). - join( '\n' )} + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} """ }} %> From cbcdd79309bcf5b7f182fcbb225b74ed901049c0 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 21:00:27 +0100 Subject: [PATCH 315/344] Lint for EditorConfig --- .../.github/workflows/linting.yml | 19 ++++++++++++++++--- 1 file changed, 16 insertions(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/.github/workflows/linting.yml b/nf_core/pipeline-template/.github/workflows/linting.yml index 4570f43bda..8095dbfe20 100644 --- a/nf_core/pipeline-template/.github/workflows/linting.yml +++ b/nf_core/pipeline-template/.github/workflows/linting.yml @@ -18,7 +18,7 @@ jobs: - name: Install markdownlint run: npm install -g markdownlint-cli - name: Run Markdownlint - run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + run: markdownlint . # If the above check failed, post a comment on the PR explaining the failure - name: Post PR comment @@ -36,7 +36,7 @@ jobs: * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) * Fix the markdown errors * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` - * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml` + * Manually resolve anything left from `markdownlint .` Once you push these changes the test should pass, and you can hide this comment :+1: @@ -46,6 +46,20 @@ jobs: repo-token: ${{ secrets.GITHUB_TOKEN }} allow-repeats: false + EditorConfig: + runs-on: ubuntu-latest + steps: + - uses: actions/checkout@v2 + + - uses: actions/setup-node@v1 + with: + node-version: "10" + + - name: Install editorconfig-checker + run: npm install -g editorconfig-checker + + - name: Run ECLint check + run: editorconfig-checker -exclude README.md $(git ls-files | grep -v test) YAML: runs-on: ubuntu-latest @@ -84,7 +98,6 @@ jobs: repo-token: ${{ secrets.GITHUB_TOKEN }} allow-repeats: false - nf-core: runs-on: ubuntu-latest steps: From 6226b30ac1f80e4c6976373efd3a9c697ed1d2c8 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 21:04:01 +0100 Subject: [PATCH 316/344] Add .editorconfig --- nf_core/pipeline-template/.editorconfig | 24 ++++++++++++++++++++++++ 1 file changed, 24 insertions(+) create mode 100644 nf_core/pipeline-template/.editorconfig diff --git a/nf_core/pipeline-template/.editorconfig b/nf_core/pipeline-template/.editorconfig new file mode 100644 index 0000000000..afb20bb1ec --- /dev/null +++ b/nf_core/pipeline-template/.editorconfig @@ -0,0 +1,24 @@ +root = true + +[*] +charset = utf-8 +end_of_line = lf +insert_final_newline = true +trim_trailing_whitespace = true +indent_size = 4 +indent_style = space + +[*.{yml,yaml}] +indent_size = 2 + +# These files are edited and tested upstream in nf-core/modules +[/modules/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset +indent_size = unset + +[/assets/email*] +indent_size = unset From b8dc08028453577bcaa696c470282774a35c0b8d Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Wed, 26 May 2021 21:14:34 +0100 Subject: [PATCH 317/344] Fix ECLint --- nf_core/pipeline-template/conf/base.config | 2 +- .../lib/NfcoreTemplate.groovy | 2 +- .../pipeline-template/lib/WorkflowMain.groovy | 2 +- nf_core/pipeline-template/nextflow.config | 110 +++---- .../pipeline-template/nextflow_schema.json | 2 +- .../subworkflows/local/input_check.nf | 84 +++--- .../pipeline-template/workflows/pipeline.nf | 282 +++++++++--------- 7 files changed, 242 insertions(+), 242 deletions(-) diff --git a/nf_core/pipeline-template/conf/base.config b/nf_core/pipeline-template/conf/base.config index 6b5b3d9ef4..3fb7b48fca 100644 --- a/nf_core/pipeline-template/conf/base.config +++ b/nf_core/pipeline-template/conf/base.config @@ -54,4 +54,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } -} \ No newline at end of file +} diff --git a/nf_core/pipeline-template/lib/NfcoreTemplate.groovy b/nf_core/pipeline-template/lib/NfcoreTemplate.groovy index 6dd9f3f258..b6e689ec88 100755 --- a/nf_core/pipeline-template/lib/NfcoreTemplate.groovy +++ b/nf_core/pipeline-template/lib/NfcoreTemplate.groovy @@ -116,7 +116,7 @@ class NfcoreTemplate { def email_html = html_template.toString() // Render the sendmail template - def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] def sf = new File("$projectDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) diff --git a/nf_core/pipeline-template/lib/WorkflowMain.groovy b/nf_core/pipeline-template/lib/WorkflowMain.groovy index 8b790f6682..597129cb50 100755 --- a/nf_core/pipeline-template/lib/WorkflowMain.groovy +++ b/nf_core/pipeline-template/lib/WorkflowMain.groovy @@ -10,7 +10,7 @@ class WorkflowMain { public static String citation(workflow) { return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + + //"* The pipeline\n" + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index 4c3428f036..3dfda9efdc 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -17,7 +17,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - + // MultiQC options multiqc_config = null multiqc_title = null @@ -37,7 +37,7 @@ params { schema_ignore_params = 'genomes,modules' enable_conda = false singularity_pull_docker_container = false - + // Config options custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" @@ -76,59 +76,59 @@ if (!params.igenomes_ignore) { } profiles { - debug { process.beforeScript = 'echo $HOSTNAME' } - conda { - params.enable_conda = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - - } - docker { - docker.enabled = true - // Avoid this error: - // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. - // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 - // once this is established and works well, nextflow might implement this behavior as new default. - docker.runOptions = '-u \$(id -u):\$(id -g)' - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - singularity { - singularity.enabled = true - singularity.autoMounts = true - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - podman { - podman.enabled = true - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false - } - shifter { - shifter.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false - } - charliecloud { - charliecloud.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - } - test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } + debug { process.beforeScript = 'echo $HOSTNAME' } + conda { + params.enable_conda = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + + } + docker { + docker.enabled = true + // Avoid this error: + // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. + // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 + // once this is established and works well, nextflow might implement this behavior as new default. + docker.runOptions = '-u \$(id -u):\$(id -g)' + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + singularity { + singularity.enabled = true + singularity.autoMounts = true + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + podman { + podman.enabled = true + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + shifter { + shifter.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + } + charliecloud { + charliecloud.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + } + test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } } // Export these variables to prevent local Python/R libraries from conflicting with those in the container diff --git a/nf_core/pipeline-template/nextflow_schema.json b/nf_core/pipeline-template/nextflow_schema.json index 806170e7b1..f90d58f349 100644 --- a/nf_core/pipeline-template/nextflow_schema.json +++ b/nf_core/pipeline-template/nextflow_schema.json @@ -284,4 +284,4 @@ "$ref": "#/definitions/generic_options" } ] -} \ No newline at end of file +} diff --git a/nf_core/pipeline-template/subworkflows/local/input_check.nf b/nf_core/pipeline-template/subworkflows/local/input_check.nf index c743c19a05..b664bc8caf 100644 --- a/nf_core/pipeline-template/subworkflows/local/input_check.nf +++ b/nf_core/pipeline-template/subworkflows/local/input_check.nf @@ -1,42 +1,42 @@ -// -// Check input samplesheet and get read channels -// - -params.options = [:] - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channels(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channels(LinkedHashMap row) { - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - def array = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - array = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return array -} \ No newline at end of file +// +// Check input samplesheet and get read channels +// + +params.options = [:] + +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) + +workflow INPUT_CHECK { + take: + samplesheet // file: /path/to/samplesheet.csv + + main: + SAMPLESHEET_CHECK ( samplesheet ) + .splitCsv ( header:true, sep:',' ) + .map { create_fastq_channels(it) } + .set { reads } + + emit: + reads // channel: [ val(meta), [ reads ] ] +} + +// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] +def create_fastq_channels(LinkedHashMap row) { + def meta = [:] + meta.id = row.sample + meta.single_end = row.single_end.toBoolean() + + def array = [] + if (!file(row.fastq_1).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" + } + if (meta.single_end) { + array = [ meta, [ file(row.fastq_1) ] ] + } else { + if (!file(row.fastq_2).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + } + array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] + } + return array +} diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index a584c5f0ad..5ae1009e30 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -1,141 +1,141 @@ -/* -======================================================================================== - VALIDATE INPUTS -======================================================================================== -*/ - -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) - -// Validate input parameters -Workflow{{ name[0]|upper }}{{ name[1:] }}.initialise(params, log) - -// TODO nf-core: Add all file path parameters for the pipeline to the list below -// Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - -// Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } - -/* -======================================================================================== - CONFIG FILES -======================================================================================== -*/ - -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() - -/* -======================================================================================== - IMPORT LOCAL MODULES/SUBWORKFLOWS -======================================================================================== -*/ - -// Don't overwrite global params.modules, create a copy instead and use that within the main script. -def modules = params.modules.clone() - -// -// MODULE: Local to the pipeline -// -include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) - -/* -======================================================================================== - IMPORT NF-CORE MODULES/SUBWORKFLOWS -======================================================================================== -*/ - -def multiqc_options = modules['multiqc'] -multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) -include { MULTIQC } from '../modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) - -/* -======================================================================================== - RUN MAIN WORKFLOW -======================================================================================== -*/ - -// Info required for completion email and summary -def multiqc_report = [] - -workflow {{ short_name|upper }} { - - ch_software_versions = Channel.empty() - - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - ch_input - ) - - // - // MODULE: Run FastQC - // - FASTQC ( - INPUT_CHECK.out.reads - ) - ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) - - // - // MODULE: Pipeline reporting - // - ch_software_versions - .map { it -> if (it) [ it.baseName, it ] } - .groupTuple() - .map { it[1][0] } - .flatten() - .collect() - .set { ch_software_versions } - - GET_SOFTWARE_VERSIONS ( - ch_software_versions.map { it }.collect() - ) - - // - // MODULE: MultiQC - // - workflow_summary = Workflow{{ name[0]|upper }}{{ name[1:] }}.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) - ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect() - ) - multiqc_report = MULTIQC.out.report.toList() - ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) -} - -/* -======================================================================================== - COMPLETION EMAIL AND SUMMARY -======================================================================================== -*/ - -workflow.onComplete { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - NfcoreTemplate.summary(workflow, params, log) -} - -/* -======================================================================================== - THE END -======================================================================================== -*/ \ No newline at end of file +/* +======================================================================================== + VALIDATE INPUTS +======================================================================================== +*/ + +def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) + +// Validate input parameters +Workflow{{ name[0]|upper }}{{ name[1:] }}.initialise(params, log) + +// TODO nf-core: Add all file path parameters for the pipeline to the list below +// Check input path parameters to see if they exist +def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] +for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + +// Check mandatory parameters +if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } + +/* +======================================================================================== + CONFIG FILES +======================================================================================== +*/ + +ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() + +/* +======================================================================================== + IMPORT LOCAL MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +// Don't overwrite global params.modules, create a copy instead and use that within the main script. +def modules = params.modules.clone() + +// +// MODULE: Local to the pipeline +// +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) + +// +// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules +// +include { INPUT_CHECK } from '../subworkflows/local/input_check' addParams( options: [:] ) + +/* +======================================================================================== + IMPORT NF-CORE MODULES/SUBWORKFLOWS +======================================================================================== +*/ + +def multiqc_options = modules['multiqc'] +multiqc_options.args += params.multiqc_title ? Utils.joinModuleArgs(["--title \"$params.multiqc_title\""]) : '' + +// +// MODULE: Installed directly from nf-core/modules +// +include { FASTQC } from '../modules/nf-core/software/fastqc/main' addParams( options: modules['fastqc'] ) +include { MULTIQC } from '../modules/nf-core/software/multiqc/main' addParams( options: multiqc_options ) + +/* +======================================================================================== + RUN MAIN WORKFLOW +======================================================================================== +*/ + +// Info required for completion email and summary +def multiqc_report = [] + +workflow {{ short_name|upper }} { + + ch_software_versions = Channel.empty() + + // + // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // + INPUT_CHECK ( + ch_input + ) + + // + // MODULE: Run FastQC + // + FASTQC ( + INPUT_CHECK.out.reads + ) + ch_software_versions = ch_software_versions.mix(FASTQC.out.version.first().ifEmpty(null)) + + // + // MODULE: Pipeline reporting + // + ch_software_versions + .map { it -> if (it) [ it.baseName, it ] } + .groupTuple() + .map { it[1][0] } + .flatten() + .collect() + .set { ch_software_versions } + + GET_SOFTWARE_VERSIONS ( + ch_software_versions.map { it }.collect() + ) + + // + // MODULE: MultiQC + // + workflow_summary = Workflow{{ name[0]|upper }}{{ name[1:] }}.paramsSummaryMultiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) + ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(GET_SOFTWARE_VERSIONS.out.yaml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect() + ) + multiqc_report = MULTIQC.out.report.toList() + ch_software_versions = ch_software_versions.mix(MULTIQC.out.version.ifEmpty(null)) +} + +/* +======================================================================================== + COMPLETION EMAIL AND SUMMARY +======================================================================================== +*/ + +workflow.onComplete { + NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) + NfcoreTemplate.summary(workflow, params, log) +} + +/* +======================================================================================== + THE END +======================================================================================== +*/ From 861df0040f93749a29c06012bc051b7ecd7a1e0b Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 07:51:51 +0100 Subject: [PATCH 318/344] Small fixes --- nf_core/create.py | 2 +- nf_core/pipeline-template/.github/workflows/linting.yml | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/create.py b/nf_core/create.py index 29fd655085..aa6d965c34 100644 --- a/nf_core/create.py +++ b/nf_core/create.py @@ -18,7 +18,7 @@ class PipelineCreate(object): - """Creates a nf-core pipeline a la carte from the nf-core best-practise template. + """Creates a nf-core pipeline a la carte from the nf-core best-practice template. Args: name (str): Name for the pipeline. diff --git a/nf_core/pipeline-template/.github/workflows/linting.yml b/nf_core/pipeline-template/.github/workflows/linting.yml index 8095dbfe20..5a99efb260 100644 --- a/nf_core/pipeline-template/.github/workflows/linting.yml +++ b/nf_core/pipeline-template/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: * On Mac: `brew install markdownlint-cli` * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) * Fix the markdown errors - * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` + * Automatically: `markdownlint . --fix` * Manually resolve anything left from `markdownlint .` Once you push these changes the test should pass, and you can hide this comment :+1: From 088a0469f560df780b7bbc92c2ea700b4cf75e57 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 08:16:10 +0100 Subject: [PATCH 319/344] Change name to short_name --- nf_core/pipeline-template/workflows/pipeline.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index 5ae1009e30..988e1545f2 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -7,7 +7,7 @@ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters -Workflow{{ name[0]|upper }}{{ name[1:] }}.initialise(params, log) +Workflow{{ short_name[0]|upper }}{{ short_name[1:] }}.initialise(params, log) // TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist @@ -106,7 +106,7 @@ workflow {{ short_name|upper }} { // // MODULE: MultiQC // - workflow_summary = Workflow{{ name[0]|upper }}{{ name[1:] }}.paramsSummaryMultiqc(workflow, summary_params) + workflow_summary = Workflow{{ short_name[0]|upper }}{{ short_name[1:] }}.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty() From e363d9dcfe759c566cb06b1d57795e93cac6274f Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 08:17:14 +0100 Subject: [PATCH 320/344] Rename pipeline.nf with Jinja2 variables --- .../workflows/{pipeline.nf => {{ short_name }}.nf} | 0 1 file changed, 0 insertions(+), 0 deletions(-) rename nf_core/pipeline-template/workflows/{pipeline.nf => {{ short_name }}.nf} (100%) diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/{{ short_name }}.nf similarity index 100% rename from nf_core/pipeline-template/workflows/pipeline.nf rename to nf_core/pipeline-template/workflows/{{ short_name }}.nf From 5b8579b9af4953a2698a3047b89d1071affa38f8 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 08:29:12 +0100 Subject: [PATCH 321/344] Rename pipeline.nf --- .../workflows/{{{ short_name }}.nf => pipeline.nf} | 0 1 file changed, 0 insertions(+), 0 deletions(-) rename nf_core/pipeline-template/workflows/{{{ short_name }}.nf => pipeline.nf} (100%) diff --git a/nf_core/pipeline-template/workflows/{{ short_name }}.nf b/nf_core/pipeline-template/workflows/pipeline.nf similarity index 100% rename from nf_core/pipeline-template/workflows/{{ short_name }}.nf rename to nf_core/pipeline-template/workflows/pipeline.nf From 4864c96a3bc2a11a1150c24a9e843b4134c00890 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 08:40:07 +0100 Subject: [PATCH 322/344] Dont .gitignore tests/ --- nf_core/pipeline-template/.gitignore | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/.gitignore b/nf_core/pipeline-template/.gitignore index aa4bb5b375..5124c9ac77 100644 --- a/nf_core/pipeline-template/.gitignore +++ b/nf_core/pipeline-template/.gitignore @@ -3,7 +3,6 @@ work/ data/ results/ .DS_Store -tests/ testing/ testing* *.pyc From 9827413ef1aee6208a7bd0d95d6ad17781670c73 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 09:13:03 +0100 Subject: [PATCH 323/344] Change location of .markdownlint.yml and update CI --- .github/workflows/markdown-lint.yml | 8 ++++---- .github/markdownlint.yml => .markdownlint.yml | 5 +++-- 2 files changed, 7 insertions(+), 6 deletions(-) rename .github/markdownlint.yml => .markdownlint.yml (88%) diff --git a/.github/workflows/markdown-lint.yml b/.github/workflows/markdown-lint.yml index 3e2158c9f4..193052a437 100644 --- a/.github/workflows/markdown-lint.yml +++ b/.github/workflows/markdown-lint.yml @@ -9,13 +9,13 @@ jobs: - uses: actions/setup-node@v1 with: - node-version: '10' + node-version: "10" - name: Install markdownlint run: npm install -g markdownlint-cli - name: Run Markdownlint - run: markdownlint ${GITHUB_WORKSPACE} -c ${GITHUB_WORKSPACE}/.github/markdownlint.yml + run: markdownlint . # If the above check failed, post a comment on the PR explaining the failure - name: Post PR comment @@ -32,8 +32,8 @@ jobs: * On Mac: `brew install markdownlint-cli` * Everything else: [Install `npm`](https://www.npmjs.com/get-npm) then [install `markdownlint-cli`](https://www.npmjs.com/package/markdownlint-cli) (`npm install -g markdownlint-cli`) * Fix the markdown errors - * Automatically: `markdownlint . --config .github/markdownlint.yml --fix` - * Manually resolve anything left from `markdownlint . --config .github/markdownlint.yml` + * Automatically: `markdownlint . --fix` + * Manually resolve anything left from `markdownlint .` Once you push these changes the test should pass, and you can hide this comment :+1: diff --git a/.github/markdownlint.yml b/.markdownlint.yml similarity index 88% rename from .github/markdownlint.yml rename to .markdownlint.yml index 2e64358780..8a3490bcbd 100644 --- a/.github/markdownlint.yml +++ b/.markdownlint.yml @@ -1,6 +1,8 @@ # Markdownlint configuration file default: true line-length: false +ul-indent: + indent: 4 no-duplicate-header: siblings_only: true no-inline-html: @@ -10,8 +12,7 @@ no-inline-html: - kbd - details - summary - - kbd # tools only - the {{ jinja variables }} break URLs and cause this to error no-bare-urls: false # tools only - suppresses error messages for usage of $ in main README -commands-show-output: false \ No newline at end of file +commands-show-output: false From 32a33c4604db1251a54f77c11a2656075f65bd08 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 09:18:51 +0100 Subject: [PATCH 324/344] Fix markdownlint --- CHANGELOG.md | 180 +++++++++++++++++++++++++-------------------------- README.md | 34 +++++----- 2 files changed, 107 insertions(+), 107 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index f9ba8f074a..d065984f72 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -38,7 +38,7 @@ * Fix bug in `nf-core download` where image names were getting a hyphen in `nf-core` which was breaking things. * Extensive new interactive prompts for all command line flags [[#1027](https://github.com/nf-core/tools/issues/1027)] - * It is now recommended to run `nf-core download` without any cli options and follow prompts (though flags can be used to run non-interactively if you wish) + * It is now recommended to run `nf-core download` without any cli options and follow prompts (though flags can be used to run non-interactively if you wish) * New helper code to set `$NXF_SINGULARITY_CACHEDIR` and add to `.bashrc` if desired [[#1027](https://github.com/nf-core/tools/issues/1027)] ### Launch @@ -103,25 +103,25 @@ ### Template * **Major new feature** - Validation of pipeline parameters [[#426]](https://github.com/nf-core/tools/issues/426) - * The addition runs as soon as the pipeline launches and checks the pipeline input parameters two main things: - * No parameters are supplied that share a name with core Nextflow options (eg. `--resume` instead of `-resume`) - * Supplied parameters validate against the pipeline JSON schema (eg. correct variable types, required values) - * If either parameter validation fails or the pipeline has errors, a warning is given about any unexpected parameters found which are not described in the pipeline schema. - * This behaviour can be disabled by using `--validate_params false` + * The addition runs as soon as the pipeline launches and checks the pipeline input parameters two main things: + * No parameters are supplied that share a name with core Nextflow options (eg. `--resume` instead of `-resume`) + * Supplied parameters validate against the pipeline JSON schema (eg. correct variable types, required values) + * If either parameter validation fails or the pipeline has errors, a warning is given about any unexpected parameters found which are not described in the pipeline schema. + * This behaviour can be disabled by using `--validate_params false` * Added profiles to support the [Charliecloud](https://hpc.github.io/charliecloud/) and [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) container engines [[#824](https://github.com/nf-core/tools/issues/824)] - * Note that Charliecloud requires Nextflow version `v21.03.0-edge` or later. + * Note that Charliecloud requires Nextflow version `v21.03.0-edge` or later. * Profiles for container engines now explicitly _disable_ all other engines [[#867](https://github.com/nf-core/tools/issues/867)] * Fixed typo in nf-core-lint CI that prevented the markdown summary from being automatically posted on PRs as a comment. * Changed default for `--input` from `data/*{1,2}.fastq.gz` to `null`, as this is now validated by the schema as a required value. * Removed support for `--name` parameter for custom run names. - * The same functionality for MultiQC still exists with the core Nextflow `-name` option. + * The same functionality for MultiQC still exists with the core Nextflow `-name` option. * Added to template docs about how to identify process name for resource customisation * The parameters `--max_memory` and `--max_time` are now validated against a regular expression [[#793](https://github.com/nf-core/tools/issues/793)] - * Must be written in the format `123.GB` / `456.h` with any of the prefixes listed in the [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#memory) - * Bare numbers no longer allowed, avoiding people from trying to specify GB and actually specifying bytes. + * Must be written in the format `123.GB` / `456.h` with any of the prefixes listed in the [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#memory) + * Bare numbers no longer allowed, avoiding people from trying to specify GB and actually specifying bytes. * Switched from cookiecutter to Jinja2 [[#880]](https://github.com/nf-core/tools/pull/880) * Finally dropped the wonderful [cookiecutter](https://github.com/cookiecutter/cookiecutter) library that was behind the first pipeline template that led to nf-core [[#880](https://github.com/nf-core/tools/pull/880)] - * Now rendering templates directly using [Jinja](https://jinja.palletsprojects.com/), which is what cookiecutter was doing anyway + * Now rendering templates directly using [Jinja](https://jinja.palletsprojects.com/), which is what cookiecutter was doing anyway ### Modules @@ -140,19 +140,19 @@ You can read more about each of these commands in the main tools documentation ( * Fixed some bugs in the command line interface for `nf-core launch` and improved formatting [[#829](https://github.com/nf-core/tools/pull/829)] * New functionality for `nf-core download` to make it compatible with DSL2 pipelines [[#832](https://github.com/nf-core/tools/pull/832)] - * Singularity images in module files are now discovered and fetched - * Direct downloads of Singularity images in python allowed (much faster than running `singularity pull`) - * Downloads now work with `$NXF_SINGULARITY_CACHEDIR` so that pipelines sharing containers have efficient downloads + * Singularity images in module files are now discovered and fetched + * Direct downloads of Singularity images in python allowed (much faster than running `singularity pull`) + * Downloads now work with `$NXF_SINGULARITY_CACHEDIR` so that pipelines sharing containers have efficient downloads * Changed behaviour of `nf-core sync` command [[#787](https://github.com/nf-core/tools/issues/787)] - * Instead of opening or updating a PR from `TEMPLATE` directly to `dev`, a new branch is now created from `TEMPLATE` and a PR opened from this to `dev`. - * This is to make it easier to fix merge conflicts without accidentally bringing the entire pipeline history back into the `TEMPLATE` branch (which makes subsequent sync merges much more difficult) + * Instead of opening or updating a PR from `TEMPLATE` directly to `dev`, a new branch is now created from `TEMPLATE` and a PR opened from this to `dev`. + * This is to make it easier to fix merge conflicts without accidentally bringing the entire pipeline history back into the `TEMPLATE` branch (which makes subsequent sync merges much more difficult) ### Linting * Major refactor and rewrite of pipieline linting code - * Much better code organisation and maintainability - * New automatically generated documentation using Sphinx - * Numerous new tests and functions, removal of some unnecessary tests + * Much better code organisation and maintainability + * New automatically generated documentation using Sphinx + * Numerous new tests and functions, removal of some unnecessary tests * Added lint check for merge markers [[#321]](https://github.com/nf-core/tools/issues/321) * Added new option `--fix` to automatically correct some problems detected by linting * Added validation of default params to `nf-core schema lint` [[#823](https://github.com/nf-core/tools/issues/823)] @@ -167,17 +167,17 @@ You can read more about each of these commands in the main tools documentation ( ### Template * Finished switch from `$baseDir` to `$projectDir` in `iGenomes.conf` and `main.nf` - * Main fix is for `smail_fields` which was a bug introduced in the previous release. Sorry about that! + * Main fix is for `smail_fields` which was a bug introduced in the previous release. Sorry about that! * Ported a number of small content tweaks from nf-core/eager to the template [[#786](https://github.com/nf-core/tools/issues/786)] - * Better contributing documentation, more placeholders in documentation files, more relaxed markdownlint exceptions for certain HTML tags, more content for the PR and issue templates. + * Better contributing documentation, more placeholders in documentation files, more relaxed markdownlint exceptions for certain HTML tags, more content for the PR and issue templates. ### Tools helper code * Pipeline schema: make parameters of type `range` to `number`. [[#738](https://github.com/nf-core/tools/issues/738)] * Respect `$NXF_HOME` when looking for pipelines with `nf-core list` [[#798](https://github.com/nf-core/tools/issues/798)] * Swapped PyInquirer with questionary for command line questions in `launch.py` [[#726](https://github.com/nf-core/tools/issues/726)] - * This should fix conda installation issues that some people had been hitting - * The change also allows other improvements to the UI + * This should fix conda installation issues that some people had been hitting + * The change also allows other improvements to the UI * Fix linting crash when a file deleted but not yet staged in git [[#796](https://github.com/nf-core/tools/issues/796)] ## [v1.12 - Mercury Weasel](https://github.com/nf-core/tools/releases/tag/1.12) - [2020-11-19] @@ -189,10 +189,10 @@ You can read more about each of these commands in the main tools documentation ( ### Template * Make CI comments work with PRs from forks [[#765](https://github.com/nf-core/tools/issues/765)] - * Branch protection and linting results should now show on all PRs + * Branch protection and linting results should now show on all PRs * Updated GitHub issue templates, which had stopped working * Refactored GitHub Actions so that the AWS full-scale tests are triggered after docker build is finished - * DockerHub push workflow split into two - one for dev, one for releases + * DockerHub push workflow split into two - one for dev, one for releases * Updated actions to no longer use `set-env` which is now depreciating [[#739](https://github.com/nf-core/tools/issues/739)] * Added config import for `test_full` in `nextflow.config` * Switched depreciated `$baseDir` to `$projectDir` @@ -234,13 +234,13 @@ Apologies for the inconvenience. * Fix syntax error in `/push_dockerhub.yml` GitHub Action workflow * Change `params.readPaths` -> `params.input_paths` in `test_full.config` * Check results when posting the lint results as a GitHub comment - * This feature is unfortunately not possible when making PRs from forks outside of the nf-core organisation for now. + * This feature is unfortunately not possible when making PRs from forks outside of the nf-core organisation for now. * More major refactoring of the automated pipeline sync - * New GitHub Actions matrix parallelisation of sync jobs across pipelines [[#673](https://github.com/nf-core/tools/issues/673)] - * Removed the `--all` behaviour from `nf-core sync` as we no longer need it - * Sync now uses a new list of pipelines on the website which does not include archived pipelines [[#712](https://github.com/nf-core/tools/issues/712)] - * When making a PR it checks if a PR already exists - if so it updates it [[#710](https://github.com/nf-core/tools/issues/710)] - * More tests and code refactoring for more stable code. Hopefully fixes 404 error [[#711](https://github.com/nf-core/tools/issues/711)] + * New GitHub Actions matrix parallelisation of sync jobs across pipelines [[#673](https://github.com/nf-core/tools/issues/673)] + * Removed the `--all` behaviour from `nf-core sync` as we no longer need it + * Sync now uses a new list of pipelines on the website which does not include archived pipelines [[#712](https://github.com/nf-core/tools/issues/712)] + * When making a PR it checks if a PR already exists - if so it updates it [[#710](https://github.com/nf-core/tools/issues/710)] + * More tests and code refactoring for more stable code. Hopefully fixes 404 error [[#711](https://github.com/nf-core/tools/issues/711)] ## [v1.10.1 - Copper Camel _(patch)_](https://github.com/nf-core/tools/releases/tag/1.10.1) - [2020-07-30] @@ -266,10 +266,10 @@ pipeline with their ID, a description, a longer help text, an optional default v The files will be used in a number of places: * Automatic validation of supplied parameters when running pipelines - * Pipeline execution can be immediately stopped if a required `param` is missing, + * Pipeline execution can be immediately stopped if a required `param` is missing, or does not conform to the patterns / allowed values in the schema. * Generation of pipeline command-line help - * Running `nextflow run --help` will use the schema to generate a help text automatically + * Running `nextflow run --help` will use the schema to generate a help text automatically * Building online documentation on the [nf-core website](https://nf-co.re) * Integration with 3rd party graphical user interfaces @@ -277,7 +277,7 @@ To support these new schema files, nf-core/tools now comes with a new set of com * Pipeline schema can be generated or updated using `nf-core schema build` - this takes the parameters from the pipeline config file and prompts the developer for any mismatch between schema and pipeline. - * Once a skeleton Schema file has been built, the command makes use of a new nf-core website tool to provide + * Once a skeleton Schema file has been built, the command makes use of a new nf-core website tool to provide a user friendly graphical interface for developers to add content to their schema: [https://nf-co.re/pipeline_schema_builder](https://nf-co.re/pipeline_schema_builder) * Pipelines will be automatically tested for valid schema that describe all pipeline parameters using the `nf-core schema lint` command (also included as part of the main `nf-core lint` command). @@ -334,7 +334,7 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Added AWS GitHub Actions workflows linting * Fail if `params.input` isn't defined. * Beautiful new progress bar to look at whilst linting is running and awesome new formatted output on the command line :heart_eyes: - * All made using the excellent [`rich` python library](https://github.com/willmcgugan/rich) - check it out! + * All made using the excellent [`rich` python library](https://github.com/willmcgugan/rich) - check it out! * Tests looking for `TODO` strings should now ignore editor backup files. [#477](https://github.com/nf-core/tools/issues/477) ### nf-core/tools Continuous Integration @@ -353,7 +353,7 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Improved command-line help text for nf-core/tools * `nf-core list` now hides archived pipelines unless `--show_archived` flag is set * Command line tools now checks if there is a new version of nf-core/tools available - * Disable this by setting the environment variable `NFCORE_NO_VERSION_CHECK`, eg. `export NFCORE_NO_VERSION_CHECK=1` + * Disable this by setting the environment variable `NFCORE_NO_VERSION_CHECK`, eg. `export NFCORE_NO_VERSION_CHECK=1` * Better command-line output formatting of nearly all `nf-core` commands using [`rich`](https://github.com/willmcgugan/rich) ## [v1.9 - Platinum Pigeon](https://github.com/nf-core/tools/releases/tag/1.9) - [2020-02-20] @@ -361,8 +361,8 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) ### Continuous integration * Travis CI tests are now deprecated in favor of GitHub Actions within the pipeline template. - * `nf-core bump-version` support has been removed for `.travis.yml` - * `nf-core lint` now fails if a `.travis.yml` file is found + * `nf-core bump-version` support has been removed for `.travis.yml` + * `nf-core lint` now fails if a `.travis.yml` file is found * Ported nf-core/tools Travis CI automation to GitHub Actions. * Fixed the build for the nf-core/tools API documentation on the website @@ -384,7 +384,7 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Improved documentation for lint errors * Allow either `>=` or `!>=` in nextflow version checks (the latter exits with an error instead of just warning) [#506](https://github.com/nf-core/tools/issues/506) * Check that `manifest.version` ends in `dev` and throw a warning if not - * If running with `--release` check the opposite and fail if not + * If running with `--release` check the opposite and fail if not * Tidied up error messages and syntax for linting GitHub actions branch tests * Add YAML validator * Don't print test results if we have a critical error @@ -404,7 +404,7 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) ### Continuous integration * GitHub Actions CI workflows are now included in the template pipeline - * Please update these files to match the existing tests that you have in `.travis.yml` + * Please update these files to match the existing tests that you have in `.travis.yml` * Travis CI tests will be deprecated from the next `tools` release * Linting will generate a warning if GitHub Actions workflows do not exist and if applicable to remove Travis CI workflow file i.e. `.travis.yml`. @@ -436,9 +436,9 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Change `maxMultiqcEmailFileSize` parameter to `max_multiqc_email_size` * Export conda environment in Docker file [#349](https://github.com/nf-core/tools/issues/349) * Change remaining parameters from `camelCase` to `snake_case` [#39](https://github.com/nf-core/hic/issues/39) - * `--singleEnd` to `--single_end` - * `--igenomesIgnore` to `--igenomes_ignore` - * Having the old camelCase versions of these will now throw an error + * `--singleEnd` to `--single_end` + * `--igenomesIgnore` to `--igenomes_ignore` + * Having the old camelCase versions of these will now throw an error * Add `autoMounts=true` to default singularity profile * Add in `markdownlint` checks that were being ignored by default * Disable ansi logging in the travis CI tests @@ -462,7 +462,7 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) ### Base Docker image * Use miniconda3 instead of miniconda for a Python 3k base environment - * If you still need Python 2 for your pipeline, add `conda-forge::python=2.7.4` to the dependencies in your `environment.yml` + * If you still need Python 2 for your pipeline, add `conda-forge::python=2.7.4` to the dependencies in your `environment.yml` * Update conda version to 4.7.12 ### Other @@ -559,17 +559,17 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) * Updated main help command to sort the subcommands in a more logical order * Updated readme to describe the new `nf-core launch` command * Fix bugs in `nf-core download` - * The _latest_ release is now fetched by default if not specified - * Downloaded pipeline files are now properly executable. + * The _latest_ release is now fetched by default if not specified + * Downloaded pipeline files are now properly executable. * Fixed bugs in `nf-core list` - * Sorting now works again - * Output is partially coloured (better highlighting out of date pipelines) - * Improved documentation + * Sorting now works again + * Output is partially coloured (better highlighting out of date pipelines) + * Improved documentation * Fixed bugs in `nf-core lint` - * The order of conda channels is now correct, avoiding occasional erroneous errors that packages weren't found ([#207](https://github.com/nf-core/tools/issues/207)) - * Allow edge versions in nf-core pipelines + * The order of conda channels is now correct, avoiding occasional erroneous errors that packages weren't found ([#207](https://github.com/nf-core/tools/issues/207)) + * Allow edge versions in nf-core pipelines * Add reporting of ignored errored process - * As a solution for [#103](https://github.com/nf-core/tools/issues/103)) + * As a solution for [#103](https://github.com/nf-core/tools/issues/103)) * Add Bowtie2 and BWA in iGenome config file template ## [v1.5 - Iron Shark](https://github.com/nf-core/tools/releases/tag/1.5) - [2019-03-13] @@ -592,26 +592,26 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) ### Tools helper code * New `nf-core launch` command to interactively launch nf-core pipelines from command-line - * Works with a `parameters.settings.json` file shipped with each pipeline - * Discovers additional `params` from the pipeline dynamically + * Works with a `parameters.settings.json` file shipped with each pipeline + * Discovers additional `params` from the pipeline dynamically * Drop Python 3.4 support * `nf-core list` now only shows a value for _"is local latest version"_ column if there is a local copy. * Lint markdown formatting in automated tests - * Added `markdownlint-cli` for checking Markdown syntax in pipelines and tools repo + * Added `markdownlint-cli` for checking Markdown syntax in pipelines and tools repo * Syncing now reads from a `blacklist.json` in order to exclude pipelines from being synced if necessary. * Added nf-core tools API description to assist developers with the classes and functions available. - * Docs are automatically built by Travis CI and updated on the nf-co.re website. + * Docs are automatically built by Travis CI and updated on the nf-co.re website. * Introduced test for filtering remote workflows by keyword. * Build tools python API docs - * Use Travis job for api doc generation and publish + * Use Travis job for api doc generation and publish * `nf-core bump-version` now stops before making changes if the linting fails * Code test coverage - * Introduced test for filtering remote workflows by keyword + * Introduced test for filtering remote workflows by keyword * Linting updates - * Now properly searches for conda packages in default channels - * Now correctly validates version pinning for packages from PyPI - * Updates for changes to `process.container` definition + * Now properly searches for conda packages in default channels + * Now correctly validates version pinning for packages from PyPI + * Updates for changes to `process.container` definition ### Other @@ -622,12 +622,12 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) ### Template pipeline * Institutional custom config profiles moved to github `nf-core/configs` - * These will now be maintained centrally as opposed to being shipped with the pipelines in `conf/` - * Load `base.config` by default for all profiles - * Removed profiles named `standard` and `none` - * Added parameter `--igenomesIgnore` so `igenomes.config` is not loaded if parameter clashes are observed - * Added parameter `--custom_config_version` for custom config version control. Can use this parameter to provide commit id for reproducibility. Defaults to `master` - * Deleted custom configs from template in `conf/` directory i.e. `uzh.config`, `binac.config` and `cfc.config` + * These will now be maintained centrally as opposed to being shipped with the pipelines in `conf/` + * Load `base.config` by default for all profiles + * Removed profiles named `standard` and `none` + * Added parameter `--igenomesIgnore` so `igenomes.config` is not loaded if parameter clashes are observed + * Added parameter `--custom_config_version` for custom config version control. Can use this parameter to provide commit id for reproducibility. Defaults to `master` + * Deleted custom configs from template in `conf/` directory i.e. `uzh.config`, `binac.config` and `cfc.config` * `multiqc_config` and `output_md` are now put into channels instead of using the files directly (see issue [#222](https://github.com/nf-core/tools/issues/222)) * Added `local.md` to cookiecutter template in `docs/configuration/`. This was referenced in `README.md` but not present. * Major overhaul of docs to add/remove parameters, unify linking of files and added description for providing custom configs where necessary @@ -645,32 +645,32 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) ## [v1.3 - Citreous Swordfish](https://github.com/nf-core/tools/releases/tag/1.3) - [2018-11-21] * `nf-core create` command line interface updated - * Interactive prompts for required arguments if not given - * New flag for workflow author + * Interactive prompts for required arguments if not given + * New flag for workflow author * Updated channel order for bioconda/conda-forge channels in environment.yaml * Increased code coverage for sub command `create` and `licenses` * Fixed nasty dependency hell issue between `pytest` and `py` package in Python 3.4.x * Introduced `.coveragerc` for pytest-cov configuration, which excludes the pipeline template now from being reported * Fix [189](https://github.com/nf-core/tools/issues/189): Check for given conda and PyPi package dependencies, if their versions exist * Added profiles for `cfc`,`binac`, `uzh` that can be synced across pipelines - * Ordering alphabetically for profiles now + * Ordering alphabetically for profiles now * Added `pip install --upgrade pip` to `.travis.yml` to update pip in the Travis CI environment ## [v1.2](https://github.com/nf-core/tools/releases/tag/1.2) - [2018-10-01] * Updated the `nf-core release` command - * Now called `nf-core bump-versions` instead - * New flag `--nextflow` to change the required nextflow version instead + * Now called `nf-core bump-versions` instead + * New flag `--nextflow` to change the required nextflow version instead * Template updates - * Simpler installation of the `nf-core` helper tool, now directly from PyPI - * Bump minimum nextflow version to `0.32.0` - required for built in `manifest.nextflowVersion` check and access to `workflow.manifest` variables from within nextflow scripts - * New `withName` syntax for configs - * Travis tests fail if PRs come against the `master` branch, slightly refactored - * Improved GitHub contributing instructions and pull request / issue templates + * Simpler installation of the `nf-core` helper tool, now directly from PyPI + * Bump minimum nextflow version to `0.32.0` - required for built in `manifest.nextflowVersion` check and access to `workflow.manifest` variables from within nextflow scripts + * New `withName` syntax for configs + * Travis tests fail if PRs come against the `master` branch, slightly refactored + * Improved GitHub contributing instructions and pull request / issue templates * New lint tests - * `.travis.yml` test for PRs made against the `master` branch - * Automatic `--release` option not used if the travis repo is `nf-core/tools` - * Warnings if depreciated variables `params.version` and `params.nf_required_version` are found + * `.travis.yml` test for PRs made against the `master` branch + * Automatic `--release` option not used if the travis repo is `nf-core/tools` + * Warnings if depreciated variables `params.version` and `params.nf_required_version` are found * New `nf-core licences` subcommand to show licence for each conda package in a workflow * `nf-core list` now has options for sorting pipeline nicely * Latest version of conda used in nf-core base docker image @@ -682,20 +682,20 @@ making a pull-request. See [`.github/CONTRIBUTING.md`](.github/CONTRIBUTING.md) Very large release containing lots of work from the first nf-core hackathon, held in SciLifeLab Stockholm. * The [Cookiecutter template](https://github.com/nf-core/cookiecutter) has been merged into tools - * The old repo above has been archived - * New pipelines are now created using the command `nf-core create` - * The nf-core template and associated linting are now controlled under the same version system + * The old repo above has been archived + * New pipelines are now created using the command `nf-core create` + * The nf-core template and associated linting are now controlled under the same version system * Large number of template updates and associated linting changes - * New simplified cookiecutter variable usage - * Refactored documentation - simplified and reduced duplication - * Better `manifest` variables instead of `params` for pipeline name and version - * New integrated nextflow version checking - * Updated travis docker pull command to use tagging to allow release tests to pass - * Reverted Docker and Singularity syntax to use `ENV` hack again + * New simplified cookiecutter variable usage + * Refactored documentation - simplified and reduced duplication + * Better `manifest` variables instead of `params` for pipeline name and version + * New integrated nextflow version checking + * Updated travis docker pull command to use tagging to allow release tests to pass + * Reverted Docker and Singularity syntax to use `ENV` hack again * Improved Python readme parsing for PyPI * Updated Travis tests to check that the correct `dev` branch is being targeted * New sync tool to automate pipeline updates - * Once initial merges are complete, a nf-core bot account will create PRs for future template updates + * Once initial merges are complete, a nf-core bot account will create PRs for future template updates ## [v1.0.1](https://github.com/nf-core/tools/releases/tag/1.0.1) - [2018-07-18] diff --git a/README.md b/README.md index ba57848a74..e77bb0319b 100644 --- a/README.md +++ b/README.md @@ -25,12 +25,12 @@ A python package with helper tools for the nf-core community. * [`nf-core bump-version` - Update nf-core pipeline version number](#bumping-a-pipeline-version-number) * [`nf-core sync` - Synchronise pipeline TEMPLATE branches](#sync-a-pipeline-with-the-template) * [`nf-core modules` - commands for dealing with DSL2 modules](#modules) - * [`modules list` - List available modules](#list-modules) - * [`modules install` - Install a module from nf-core/modules](#install-a-module-into-a-pipeline) - * [`modules remove` - Remove a module from a pipeline](#remove-a-module-from-a-pipeline) - * [`modules create` - Create a module from the template](#create-a-new-module) - * [`modules create-test-yml` - Create the `test.yml` file for a module](#create-a-module-test-config-file) - * [`modules lint` - Check a module against nf-core guidelines](#check-a-module-against-nf-core-guidelines) + * [`modules list` - List available modules](#list-modules) + * [`modules install` - Install a module from nf-core/modules](#install-a-module-into-a-pipeline) + * [`modules remove` - Remove a module from a pipeline](#remove-a-module-from-a-pipeline) + * [`modules create` - Create a module from the template](#create-a-new-module) + * [`modules create-test-yml` - Create the `test.yml` file for a module](#create-a-module-test-config-file) + * [`modules lint` - Check a module against nf-core guidelines](#check-a-module-against-nf-core-guidelines) * [Citation](#citation) The nf-core tools package is written in Python and can be imported and used within other packages. @@ -327,24 +327,24 @@ Do you want to run this command now? [y/n]: ### Launch tool options * `-r`, `--revision` - * Specify a pipeline release (or branch / git commit sha) of the project to run + * Specify a pipeline release (or branch / git commit sha) of the project to run * `-i`, `--id` - * You can use the web GUI for nf-core pipelines by clicking _"Launch"_ on the website. Once filled in you will be given an ID to use with this command which is used to retrieve your inputs. + * You can use the web GUI for nf-core pipelines by clicking _"Launch"_ on the website. Once filled in you will be given an ID to use with this command which is used to retrieve your inputs. * `-c`, `--command-only` - * If you prefer not to save your inputs in a JSON file and use `-params-file`, this option will specify all entered params directly in the nextflow command. + * If you prefer not to save your inputs in a JSON file and use `-params-file`, this option will specify all entered params directly in the nextflow command. * `-p`, `--params-in PATH` - * To use values entered in a previous pipeline run, you can supply the `nf-params.json` file previously generated. - * This will overwrite the pipeline schema defaults before the wizard is launched. + * To use values entered in a previous pipeline run, you can supply the `nf-params.json` file previously generated. + * This will overwrite the pipeline schema defaults before the wizard is launched. * `-o`, `--params-out PATH` - * Path to save parameters JSON file to. (Default: `nf-params.json`) + * Path to save parameters JSON file to. (Default: `nf-params.json`) * `-a`, `--save-all` - * Without this option the pipeline will ignore any values that match the pipeline schema defaults. - * This option saves _all_ parameters found to the JSON file. + * Without this option the pipeline will ignore any values that match the pipeline schema defaults. + * This option saves _all_ parameters found to the JSON file. * `-h`, `--show-hidden` - * A pipeline JSON schema can define some parameters as 'hidden' if they are rarely used or for internal pipeline use only. - * This option forces the wizard to show all parameters, including those labelled as 'hidden'. + * A pipeline JSON schema can define some parameters as 'hidden' if they are rarely used or for internal pipeline use only. + * This option forces the wizard to show all parameters, including those labelled as 'hidden'. * `--url` - * Change the URL used for the graphical interface, useful for development work on the website. + * Change the URL used for the graphical interface, useful for development work on the website. ## Downloading pipelines for offline use From 8b45ab9346c5e7d555018268fe7774aad308dcbd Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 09:53:00 +0100 Subject: [PATCH 325/344] Rename main pipeline files --- nf_core/create.py | 9 +++++++-- 1 file changed, 7 insertions(+), 2 deletions(-) diff --git a/nf_core/create.py b/nf_core/create.py index aa6d965c34..3f2f0d19c5 100644 --- a/nf_core/create.py +++ b/nf_core/create.py @@ -16,7 +16,6 @@ log = logging.getLogger(__name__) - class PipelineCreate(object): """Creates a nf-core pipeline a la carte from the nf-core best-practice template. @@ -89,7 +88,11 @@ def render_template(self): template_files = list(pathlib.Path(template_dir).glob("**/*")) template_files += list(pathlib.Path(template_dir).glob("*")) ignore_strs = [".pyc", "__pycache__", ".pyo", ".pyd", ".DS_Store", ".egg"] - + rename_files = { + 'workflows/pipeline.nf': f"workflows/{self.short_name}.nf", + 'lib/WorkflowPipeline.groovy': f"lib/Workflow{self.short_name[0].upper()}{self.short_name[1:]}.groovy" + } + for template_fn_path_obj in template_files: template_fn_path = str(template_fn_path_obj) @@ -102,6 +105,8 @@ def render_template(self): # Set up vars and directories template_fn = os.path.relpath(template_fn_path, template_dir) output_path = os.path.join(self.outdir, template_fn) + if template_fn in rename_files: + output_path = os.path.join(self.outdir, rename_files[template_fn]) os.makedirs(os.path.dirname(output_path), exist_ok=True) try: From 759abeddc9a87ed68d60ae5d97ad8c9e55fa0205 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 10:01:00 +0100 Subject: [PATCH 326/344] Change files_exist.py with updated files --- nf_core/lint/files_exist.py | 16 ++++++++-------- 1 file changed, 8 insertions(+), 8 deletions(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index b0f07f5105..3c89134250 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -20,12 +20,12 @@ def files_exist(self): .gitattributes .gitignore + .markdownlint.yml .github/.dockstore.yml .github/CONTRIBUTING.md .github/ISSUE_TEMPLATE/bug_report.md .github/ISSUE_TEMPLATE/config.yml .github/ISSUE_TEMPLATE/feature_request.md - .github/markdownlint.yml .github/PULL_REQUEST_TEMPLATE.md .github/workflows/branch.yml .github/workflows/ci.yml @@ -47,12 +47,11 @@ def files_exist(self): docs/output.md docs/README.md docs/usage.md - lib/Checks.groovy - lib/Completion.groovy lib/nfcore_external_java_deps.jar lib/NfcoreSchema.groovy + lib/NfcoreTemplate.groovy lib/Utils.groovy - lib/Workflow.groovy + lib/WorkflowMain.groovy modules/local/get_software_versions.nf nextflow_schema.json nextflow.config @@ -70,6 +69,7 @@ def files_exist(self): conf/igenomes.config .github/workflows/awstest.yml .github/workflows/awsfulltest.yml + lib/WorkflowPIPELINE.groovy Files that *must not* be present: @@ -100,6 +100,7 @@ def files_exist(self): files_fail = [ [".gitattributes"], [".gitignore"], + [".markdownlint.yml"], ["CHANGELOG.md"], ["CITATIONS.md"], ["CODE_OF_CONDUCT.md"], @@ -113,7 +114,6 @@ def files_exist(self): [os.path.join(".github", "ISSUE_TEMPLATE", "bug_report.md")], [os.path.join(".github", "ISSUE_TEMPLATE", "config.yml")], [os.path.join(".github", "ISSUE_TEMPLATE", "feature_request.md")], - [os.path.join(".github", "markdownlint.yml")], [os.path.join(".github", "PULL_REQUEST_TEMPLATE.md")], [os.path.join(".github", "workflows", "branch.yml")], [os.path.join(".github", "workflows", "ci.yml")], @@ -132,12 +132,11 @@ def files_exist(self): [os.path.join("docs", "README.md")], [os.path.join("docs", "README.md")], [os.path.join("docs", "usage.md")], - [os.path.join("lib", "Checks.groovy")], - [os.path.join("lib", "Completion.groovy")], [os.path.join("lib", "nfcore_external_java_deps.jar")], [os.path.join("lib", "NfcoreSchema.groovy")], + [os.path.join("lib", "NfcoreTemplate.groovy")], [os.path.join("lib", "Utils.groovy")], - [os.path.join("lib", "Workflow.groovy")], + [os.path.join("lib", "WorkflowMain.groovy")], [os.path.join("modules", "local", "get_software_versions.nf")], ] @@ -150,6 +149,7 @@ def files_exist(self): [os.path.join("conf", "igenomes.config")], [os.path.join(".github", "workflows", "awstest.yml")], [os.path.join(".github", "workflows", "awsfulltest.yml")], + [os.path.join("lib", f"Workflow{short_name[0].upper()}{short_name[1:]}.groovy"))] ] # List of strings. Fails / warns if any of the strings exist. From 34b16a0e8883dfe83d041eceff9f8c53d567d80a Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 10:03:59 +0100 Subject: [PATCH 327/344] Fix syntax error --- nf_core/lint/files_exist.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index 3c89134250..647f8538d5 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -149,7 +149,7 @@ def files_exist(self): [os.path.join("conf", "igenomes.config")], [os.path.join(".github", "workflows", "awstest.yml")], [os.path.join(".github", "workflows", "awsfulltest.yml")], - [os.path.join("lib", f"Workflow{short_name[0].upper()}{short_name[1:]}.groovy"))] + [os.path.join("lib", f"Workflow{short_name[0].upper()}{short_name[1:]}.groovy")] ] # List of strings. Fails / warns if any of the strings exist. From e0d6e5add2fccdd7dfda6aafe269e74a5b8562db Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 10:31:39 +0100 Subject: [PATCH 328/344] Fix files unchanged lint tests --- nf_core/lint/files_unchanged.py | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index 6b9d674473..a386c5c918 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -19,12 +19,12 @@ def files_unchanged(self): Files that must be unchanged:: .gitattributes + .markdownlint.yml .github/.dockstore.yml .github/CONTRIBUTING.md .github/ISSUE_TEMPLATE/bug_report.md .github/ISSUE_TEMPLATE/config.yml .github/ISSUE_TEMPLATE/feature_request.md - .github/markdownlint.yml .github/PULL_REQUEST_TEMPLATE.md .github/workflows/branch.yml .github/workflows/linting_comment.yml @@ -38,6 +38,7 @@ def files_unchanged(self): docs/README.md' lib/nfcore_external_java_deps.jar lib/NfcoreSchema.groovy + lib/NfcoreTemplate.groovy ['LICENSE', 'LICENSE.md', 'LICENCE', 'LICENCE.md'], # NB: British / American spelling Files that can have additional content but must include the template contents:: @@ -75,6 +76,7 @@ def files_unchanged(self): # List of lists. Passes if any of the files in the sublist are found. files_exact = [ [".gitattributes"], + [".markdownlint.yml")], ["CODE_OF_CONDUCT.md"], ["LICENSE", "LICENSE.md", "LICENCE", "LICENCE.md"], # NB: British / American spelling [os.path.join(".github", ".dockstore.yml")], @@ -82,7 +84,6 @@ def files_unchanged(self): [os.path.join(".github", "ISSUE_TEMPLATE", "bug_report.md")], [os.path.join(".github", "ISSUE_TEMPLATE", "config.yml")], [os.path.join(".github", "ISSUE_TEMPLATE", "feature_request.md")], - [os.path.join(".github", "markdownlint.yml")], [os.path.join(".github", "PULL_REQUEST_TEMPLATE.md")], [os.path.join(".github", "workflows", "branch.yml")], [os.path.join(".github", "workflows", "linting_comment.yml")], @@ -96,8 +97,7 @@ def files_unchanged(self): [os.path.join("docs", "README.md")], [os.path.join("lib", "nfcore_external_java_deps.jar")], [os.path.join("lib", "NfcoreSchema.groovy")], - [os.path.join("lib", "Checks.groovy")], - [os.path.join("lib", "Utils.groovy")], + [os.path.join("lib", "NfcoreTemplate.groovy")], ] files_partial = [ [".gitignore", "foo"], From 8919db904cfc5b298064da888af69184479898be Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 10:37:48 +0100 Subject: [PATCH 329/344] Arrrggghhhh syntax --- nf_core/create.py | 7 ++++--- nf_core/lint/files_exist.py | 2 +- nf_core/lint/files_unchanged.py | 2 +- 3 files changed, 6 insertions(+), 5 deletions(-) diff --git a/nf_core/create.py b/nf_core/create.py index 3f2f0d19c5..de30f8d3dd 100644 --- a/nf_core/create.py +++ b/nf_core/create.py @@ -16,6 +16,7 @@ log = logging.getLogger(__name__) + class PipelineCreate(object): """Creates a nf-core pipeline a la carte from the nf-core best-practice template. @@ -89,10 +90,10 @@ def render_template(self): template_files += list(pathlib.Path(template_dir).glob("*")) ignore_strs = [".pyc", "__pycache__", ".pyo", ".pyd", ".DS_Store", ".egg"] rename_files = { - 'workflows/pipeline.nf': f"workflows/{self.short_name}.nf", - 'lib/WorkflowPipeline.groovy': f"lib/Workflow{self.short_name[0].upper()}{self.short_name[1:]}.groovy" + "workflows/pipeline.nf": f"workflows/{self.short_name}.nf", + "lib/WorkflowPipeline.groovy": f"lib/Workflow{self.short_name[0].upper()}{self.short_name[1:]}.groovy", } - + for template_fn_path_obj in template_files: template_fn_path = str(template_fn_path_obj) diff --git a/nf_core/lint/files_exist.py b/nf_core/lint/files_exist.py index 647f8538d5..661c76847b 100644 --- a/nf_core/lint/files_exist.py +++ b/nf_core/lint/files_exist.py @@ -149,7 +149,7 @@ def files_exist(self): [os.path.join("conf", "igenomes.config")], [os.path.join(".github", "workflows", "awstest.yml")], [os.path.join(".github", "workflows", "awsfulltest.yml")], - [os.path.join("lib", f"Workflow{short_name[0].upper()}{short_name[1:]}.groovy")] + [os.path.join("lib", f"Workflow{short_name[0].upper()}{short_name[1:]}.groovy")], ] # List of strings. Fails / warns if any of the strings exist. diff --git a/nf_core/lint/files_unchanged.py b/nf_core/lint/files_unchanged.py index a386c5c918..37728b8b06 100644 --- a/nf_core/lint/files_unchanged.py +++ b/nf_core/lint/files_unchanged.py @@ -76,7 +76,7 @@ def files_unchanged(self): # List of lists. Passes if any of the files in the sublist are found. files_exact = [ [".gitattributes"], - [".markdownlint.yml")], + [".markdownlint.yml"], ["CODE_OF_CONDUCT.md"], ["LICENSE", "LICENSE.md", "LICENCE", "LICENCE.md"], # NB: British / American spelling [os.path.join(".github", ".dockstore.yml")], From 9b462aaa7bbdce5d98a6af4e2646ab42566d3889 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 10:53:15 +0100 Subject: [PATCH 330/344] Fix Semantic errors and get pipeline running --- nf_core/pipeline-template/lib/WorkflowPipeline.groovy | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/lib/WorkflowPipeline.groovy b/nf_core/pipeline-template/lib/WorkflowPipeline.groovy index b5bf141b3a..ce022c3a43 100755 --- a/nf_core/pipeline-template/lib/WorkflowPipeline.groovy +++ b/nf_core/pipeline-template/lib/WorkflowPipeline.groovy @@ -2,12 +2,12 @@ // This file holds several functions specific to the workflow/{{ short_name }}.nf in the {{ name }} pipeline // -class Workflow{{ name[0]|upper }}{{ name[1:] }} { +class Workflow{{ short_name[0]|upper }}{{ short_name[1:] }} { // // Check and validate parameters // - public static void initialise(params, log, valid_params) { + public static void initialise(params, log) { genomeExistsError(params, log) if (!params.fasta) { From cc97eb6d7e8de683aa0c0f3c31869e626170f3e5 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 12:53:41 +0100 Subject: [PATCH 331/344] Fix launch and schema test failures --- nf_core/pipeline-template/nextflow_schema.json | 5 ++++- tests/test_schema.py | 2 +- 2 files changed, 5 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/nextflow_schema.json b/nf_core/pipeline-template/nextflow_schema.json index f90d58f349..0d0dbed372 100644 --- a/nf_core/pipeline-template/nextflow_schema.json +++ b/nf_core/pipeline-template/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/{{ name }}/master/nextflow_schema.json", "title": "{{ name }} pipeline parameters", - "description": "Nextflow RNA-Seq analysis pipeline, part of the nf-core community.", + "description": "{{ description }}", "type": "object", "definitions": { "input_output_options": { @@ -10,6 +10,9 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", + "required": [ + "input" + ], "properties": { "input": { "type": "string", diff --git a/tests/test_schema.py b/tests/test_schema.py index 7d37636a0d..175b23880c 100644 --- a/tests/test_schema.py +++ b/tests/test_schema.py @@ -125,7 +125,7 @@ def test_validate_params_pass(self): # Load the template schema self.schema_obj.schema_filename = self.template_schema self.schema_obj.load_schema() - self.schema_obj.input_params = {"input": "fubar"} + self.schema_obj.input_params = {"input": "fubar.csv"} assert self.schema_obj.validate_params() def test_validate_params_fail(self): From a9b2a03c8e1bd60432e690ce268881c90c088256 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 13:06:54 +0100 Subject: [PATCH 332/344] Remove rnaseq reference --- nf_core/pipeline-template/README.md | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/nf_core/pipeline-template/README.md b/nf_core/pipeline-template/README.md index 135534ba6b..c8763e132a 100644 --- a/nf_core/pipeline-template/README.md +++ b/nf_core/pipeline-template/README.md @@ -55,11 +55,9 @@ On release, automated continuous integration tests run the pipeline on a full-si nextflow run {{ name }} -profile --input samplesheet.csv --genome GRCh37 ``` -See [usage](https://nf-co.re/rnaseq/usage) and [parameter](https://nf-co.re/rnaseq/parameters) docs for all of the available options when running the pipeline. - ## Documentation -The {{ name }} pipeline comes with documentation about the pipeline: [usage](https://nf-co.re/{{ short_name }}/usage) and [output](https://nf-co.re/{{ short_name }}/output). +The {{ name }} pipeline comes with documentation about the pipeline [usage](https://nf-co.re/{{ short_name }}/usage), [parameters](https://nf-co.re/{{ short_name }}/parameters) and [output](https://nf-co.re/{{ short_name }}/output). ## Credits From 43e21b349d827a8690066601fc8e8474c70ab230 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 27 May 2021 18:31:23 +0100 Subject: [PATCH 333/344] Remove docker.runOptions --- nf_core/pipeline-template/nextflow.config | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index 3dfda9efdc..a29931b4f1 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -88,11 +88,7 @@ profiles { } docker { docker.enabled = true - // Avoid this error: - // WARNING: Your kernel does not support swap limit capabilities or the cgroup is not mounted. Memory limited without swap. - // Testing this in nf-core after discussion here https://github.com/nf-core/tools/pull/351 - // once this is established and works well, nextflow might implement this behavior as new default. - docker.runOptions = '-u \$(id -u):\$(id -g)' + docker.userEmulation = true singularity.enabled = false podman.enabled = false shifter.enabled = false From 1895b2d6bbc5ebc46a4226e1437f5ff493072fd2 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 28 May 2021 11:01:19 +0100 Subject: [PATCH 334/344] Add lint test to check NF version in quickstart @kevinmenden --- nf_core/lint/readme.py | 19 +++++++++++++++++++ 1 file changed, 19 insertions(+) diff --git a/nf_core/lint/readme.py b/nf_core/lint/readme.py index c595df074c..314156786b 100644 --- a/nf_core/lint/readme.py +++ b/nf_core/lint/readme.py @@ -60,6 +60,25 @@ def readme(self): else: warned.append("README did not have a Nextflow minimum version badge.") + # Check that the minimum version mentioned in the quick start section is consistent + # Looking for: "1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=20.04.0`)" + nf_version_re = r"1\.\s*Install\s*\[`Nextflow`\]\(https://www.nextflow.io/docs/latest/getstarted.html#installation\)\s*\(`>=(\d*\.\d*\.\d*)`\)" + match = re.search(nf_version_re, content) + if match: + nf_quickstart_version = match.group(1) + try: + assert nf_quickstart_version == self.minNextflowVersion + except (AssertionError, KeyError): + failed.append( + f"README Nextflow minimium version in Quick Start section does not match config. README: `{nf_quickstart_version}`, Config `{self.minNextflowVersion}`" + ) + else: + passed.append( + f"README Nextflow minimum version in Quick Start section matched config. README: `{nf_quickstart_version}`, Config: `{self.minNextflowVersion}`" + ) + else: + warned.append("README did not have a Nextflow minimum version mentioned in Quick Start section.") + # Check that we have a bioconda badge if we have a bioconda environment file if os.path.join(self.wf_path, "environment.yml") in self.files: bioconda_badge = "[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/)" From f82705cb46c34e198aad8f115dfcfc47c160f6b0 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 28 May 2021 11:05:23 +0100 Subject: [PATCH 335/344] Black --- nf_core/lint/readme.py | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf_core/lint/readme.py b/nf_core/lint/readme.py index 314156786b..823d9c80d6 100644 --- a/nf_core/lint/readme.py +++ b/nf_core/lint/readme.py @@ -78,7 +78,7 @@ def readme(self): ) else: warned.append("README did not have a Nextflow minimum version mentioned in Quick Start section.") - + # Check that we have a bioconda badge if we have a bioconda environment file if os.path.join(self.wf_path, "environment.yml") in self.files: bioconda_badge = "[![install with bioconda](https://img.shields.io/badge/install%20with-bioconda-brightgreen.svg)](https://bioconda.github.io/)" From f4f6914d42883fc5b6406597ae31cadfee9208b1 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Fri, 28 May 2021 12:18:17 +0100 Subject: [PATCH 336/344] Remove extra line --- nf_core/pipeline-template/nextflow.config | 1 - 1 file changed, 1 deletion(-) diff --git a/nf_core/pipeline-template/nextflow.config b/nf_core/pipeline-template/nextflow.config index a29931b4f1..a0d8c15055 100644 --- a/nf_core/pipeline-template/nextflow.config +++ b/nf_core/pipeline-template/nextflow.config @@ -84,7 +84,6 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false - } docker { docker.enabled = true From fd164e6bb07811d8d8f94219a734b34f7e6e9d38 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Thu, 3 Jun 2021 15:21:01 +0100 Subject: [PATCH 337/344] Fix typ --- nf_core/pipeline-template/.github/CONTRIBUTING.md | 2 +- nf_core/pipeline-template/assets/schema_input.json | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/.github/CONTRIBUTING.md b/nf_core/pipeline-template/.github/CONTRIBUTING.md index 2efd6020bd..e416773357 100644 --- a/nf_core/pipeline-template/.github/CONTRIBUTING.md +++ b/nf_core/pipeline-template/.github/CONTRIBUTING.md @@ -74,7 +74,7 @@ If you wish to contribute a new step, please use the following coding standards: 7. Add sanity checks for all relevant parameters. 8. Add any new software to the `scrape_software_versions.py` script in `bin/` and the version command to the `scrape_software_versions` process in `main.nf`. 9. Do local tests that the new code works properly and as expected. -10. Add a new test command in `.github/workflow/ci.yaml`. +10. Add a new test command in `.github/workflow/ci.yml`. 11. If applicable add a [MultiQC](https://https://multiqc.info/) module. 12. Update MultiQC config `assets/multiqc_config.yaml` so relevant suffixes, name clean up, General Statistics Table column order, and module figures are in the right order. 13. Optional: Add any descriptions of MultiQC report sections and output files to `docs/output.md`. diff --git a/nf_core/pipeline-template/assets/schema_input.json b/nf_core/pipeline-template/assets/schema_input.json index c32a0275bf..5ce14d5825 100644 --- a/nf_core/pipeline-template/assets/schema_input.json +++ b/nf_core/pipeline-template/assets/schema_input.json @@ -1,6 +1,6 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/{{ name }}/master/schema_input.json", + "$id": "https://raw.githubusercontent.com/{{ name }}/master/assets/schema_input.json", "title": "{{ name }} pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", From eac411a6f8abdf98dfd24e298d203638d42ca12a Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Mon, 7 Jun 2021 17:36:33 +0100 Subject: [PATCH 338/344] Add in camelCaseBug detection in NfcoreSchema.groovy --- nf_core/pipeline-template/lib/NfcoreSchema.groovy | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/nf_core/pipeline-template/lib/NfcoreSchema.groovy b/nf_core/pipeline-template/lib/NfcoreSchema.groovy index 16986434f7..8d6920dd64 100755 --- a/nf_core/pipeline-template/lib/NfcoreSchema.groovy +++ b/nf_core/pipeline-template/lib/NfcoreSchema.groovy @@ -121,7 +121,8 @@ class NfcoreSchema { def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !expectedParamsLowerCase.contains(specifiedParamLowerCase)) { + def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { // Temporarily remove camelCase/camel-case params #1035 def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ From c1a15bae4b72088524335e238d1a770c5c307cc9 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 15 Jun 2021 08:52:08 +0100 Subject: [PATCH 339/344] Add section to usage docs about user specificed containers --- nf_core/pipeline-template/docs/usage.md | 54 +++++++++++++++++++++---- 1 file changed, 47 insertions(+), 7 deletions(-) diff --git a/nf_core/pipeline-template/docs/usage.md b/nf_core/pipeline-template/docs/usage.md index f92475dc6f..3d75c25bfc 100644 --- a/nf_core/pipeline-template/docs/usage.md +++ b/nf_core/pipeline-template/docs/usage.md @@ -12,7 +12,7 @@ You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. -```bash +```console --input '[path to samplesheet file]' ``` @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```bash +```console sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```bash +```console sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz @@ -56,7 +56,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: -```bash +```console nextflow run {{ name }} --input samplesheet.csv --genome GRCh37 -profile docker ``` @@ -64,7 +64,7 @@ This will launch the pipeline with the `docker` configuration profile. See below Note that the pipeline will create the following files in your working directory: -```bash +```console work # Directory containing the nextflow working files results # Finished results (configurable, see below) .nextflow_log # Log file from Nextflow @@ -75,7 +75,7 @@ results # Finished results (configurable, see below) When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: -```bash +```console nextflow pull {{ name }} ``` @@ -217,6 +217,46 @@ params { } ``` +### Updating containers + +The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. + +1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19) +2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) +3. Create the custom config accordingly: + + * For Docker: + + ```nextflow + process { + withName: PANGOLIN { + container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + + * For Singularity: + + ```nextflow + process { + withName: PANGOLIN { + container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' + } + } + ``` + + * For Conda: + + ```nextflow + process { + withName: PANGOLIN { + conda = 'bioconda::pangolin=3.0.5' + } + } + ``` + +> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. + ### nf-core/configs In most cases, you will only need to create a custom config as a one-off but if you and others within your organisation are likely to be running nf-core pipelines regularly and need to use the same settings regularly it may be a good idea to request that your custom config file is uploaded to the `nf-core/configs` git repository. Before you do this please can you test that the config file works with your pipeline of choice using the `-c` parameter. You can then create a pull request to the `nf-core/configs` repository with the addition of your config file, associated documentation file (see examples in [`nf-core/configs/docs`](https://github.com/nf-core/configs/tree/master/docs)), and amending [`nfcore_custom.config`](https://github.com/nf-core/configs/blob/master/nfcore_custom.config) to include your custom profile. @@ -239,6 +279,6 @@ Some HPC setups also allow you to run nextflow within a cluster job submitted yo In some cases, the Nextflow Java virtual machines can start to request a large amount of memory. We recommend adding the following line to your environment to limit this (typically in `~/.bashrc` or `~./bash_profile`): -```bash +```console NXF_OPTS='-Xms1g -Xmx4g' ``` From c8a4b8603ebfa6d5447c26352d0ebac9cc582f85 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 15 Jun 2021 08:55:41 +0100 Subject: [PATCH 340/344] Auto-replace spaces with underscores in sample names --- nf_core/pipeline-template/bin/check_samplesheet.py | 6 ++---- nf_core/pipeline-template/docs/usage.md | 10 +++++----- 2 files changed, 7 insertions(+), 9 deletions(-) diff --git a/nf_core/pipeline-template/bin/check_samplesheet.py b/nf_core/pipeline-template/bin/check_samplesheet.py index fe0e92c33f..35ae64ee41 100755 --- a/nf_core/pipeline-template/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/bin/check_samplesheet.py @@ -85,10 +85,8 @@ def check_samplesheet(file_in, file_out): ## Check sample name entries sample, fastq_1, fastq_2 = lspl[: len(HEADER)] - if sample: - if sample.find(" ") != -1: - print_error("Sample entry contains spaces!", "Line", line) - else: + sample = sample.replace(' ', '_') + if not sample: print_error("Sample entry has not been specified!", "Line", line) ## Check FastQ file extension diff --git a/nf_core/pipeline-template/docs/usage.md b/nf_core/pipeline-template/docs/usage.md index 3d75c25bfc..b4a708d8ec 100644 --- a/nf_core/pipeline-template/docs/usage.md +++ b/nf_core/pipeline-template/docs/usage.md @@ -44,11 +44,11 @@ TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, ``` -| Column | Description | -|----------------|-----------------------------------------------------------------------------------------------------------------------------| -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| Column | Description | +|----------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. From be2d4d7b437491301449fdbd936cd73bb4411741 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 15 Jun 2021 08:56:01 +0100 Subject: [PATCH 341/344] Move include statement out of workflow section in main.nf --- nf_core/pipeline-template/main.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/nf_core/pipeline-template/main.nf b/nf_core/pipeline-template/main.nf index afe5199f24..dca79e2684 100644 --- a/nf_core/pipeline-template/main.nf +++ b/nf_core/pipeline-template/main.nf @@ -33,12 +33,12 @@ WorkflowMain.initialise(workflow, params, log) ======================================================================================== */ -workflow NFCORE_{{ short_name|upper }} { +include { {{ short_name|upper }} } from './workflows/{{ short_name }}' - // - // WORKFLOW: Run main {{ name }} analysis pipeline - // - include { {{ short_name|upper }} } from './workflows/{{ short_name }}' +// +// WORKFLOW: Run main {{ name }} analysis pipeline +// +workflow NFCORE_{{ short_name|upper }} { {{ short_name|upper }} () } From a569b274c496b0b74083f0fddf639d67d203ef4d Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 15 Jun 2021 08:56:42 +0100 Subject: [PATCH 342/344] software_versions is a tsv and not csv --- nf_core/pipeline-template/bin/scrape_software_versions.py | 2 +- nf_core/pipeline-template/docs/output.md | 2 +- .../pipeline-template/modules/local/get_software_versions.nf | 2 +- nf_core/pipeline-template/workflows/pipeline.nf | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/nf_core/pipeline-template/bin/scrape_software_versions.py b/nf_core/pipeline-template/bin/scrape_software_versions.py index 6b4c1b6981..c55be87266 100755 --- a/nf_core/pipeline-template/bin/scrape_software_versions.py +++ b/nf_core/pipeline-template/bin/scrape_software_versions.py @@ -31,6 +31,6 @@ print(" ") # Write out regexes as csv file: -with open("software_versions.csv", "w") as f: +with open("software_versions.tsv", "w") as f: for k, v in sorted(results.items()): f.write("{}\t{}\n".format(k, v)) diff --git a/nf_core/pipeline-template/docs/output.md b/nf_core/pipeline-template/docs/output.md index 621733c835..9646e12290 100644 --- a/nf_core/pipeline-template/docs/output.md +++ b/nf_core/pipeline-template/docs/output.md @@ -60,7 +60,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ * `pipeline_info/` * Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.csv`. + * Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.tsv`. * Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. diff --git a/nf_core/pipeline-template/modules/local/get_software_versions.nf b/nf_core/pipeline-template/modules/local/get_software_versions.nf index a5a819487c..8af8af1735 100644 --- a/nf_core/pipeline-template/modules/local/get_software_versions.nf +++ b/nf_core/pipeline-template/modules/local/get_software_versions.nf @@ -21,7 +21,7 @@ process GET_SOFTWARE_VERSIONS { path versions output: - path "software_versions.csv" , emit: csv + path "software_versions.tsv" , emit: tsv path 'software_versions_mqc.yaml', emit: yaml script: // This script is bundled with the pipeline, in {{ name }}/bin/ diff --git a/nf_core/pipeline-template/workflows/pipeline.nf b/nf_core/pipeline-template/workflows/pipeline.nf index 988e1545f2..f6b86faf0b 100644 --- a/nf_core/pipeline-template/workflows/pipeline.nf +++ b/nf_core/pipeline-template/workflows/pipeline.nf @@ -38,7 +38,7 @@ def modules = params.modules.clone() // // MODULE: Local to the pipeline // -include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['csv':'']] ) +include { GET_SOFTWARE_VERSIONS } from '../modules/local/get_software_versions' addParams( options: [publish_files : ['tsv':'']] ) // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules From 25a52f8f294714b61cbb07a5874c8b74bb58b341 Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 15 Jun 2021 09:14:27 +0100 Subject: [PATCH 343/344] Add TODO statements to uncommented code --- tests/test_licenses.py | 2 ++ tests/test_lint.py | 1 + tests/test_utils.py | 2 ++ 3 files changed, 5 insertions(+) diff --git a/tests/test_licenses.py b/tests/test_licenses.py index 620bf5486d..59ea08f7f3 100644 --- a/tests/test_licenses.py +++ b/tests/test_licenses.py @@ -11,6 +11,8 @@ import nf_core.create import nf_core.licences +# TODO nf-core: Assess and strip out if no longer required for DSL2 + # class WorkflowLicensesTest(unittest.TestCase): # """A class that performs tests on the workflow license # retrieval functionality of nf-core tools.""" diff --git a/tests/test_lint.py b/tests/test_lint.py index c877fd23b7..f51d007aad 100644 --- a/tests/test_lint.py +++ b/tests/test_lint.py @@ -213,6 +213,7 @@ def test_sphinx_rst_files(self): test_files_unchanged_fail, ) +# TODO nf-core: Assess and strip out if no longer required for DSL2 # def test_critical_missingfiles_example(self): # """Tests for missing nextflow config and main.nf files""" diff --git a/tests/test_utils.py b/tests/test_utils.py index 74d258b585..08a62f723b 100644 --- a/tests/test_utils.py +++ b/tests/test_utils.py @@ -77,6 +77,8 @@ def test_load_pipeline_config(self): self.pipeline_obj._load_pipeline_config() assert self.pipeline_obj.nf_config["dag.enabled"] == "true" + # TODO nf-core: Assess and strip out if no longer required for DSL2 + # def test_load_conda_env(self): # """Load the pipeline Conda environment.yml file""" # self.pipeline_obj._load_conda_environment() From 11902dcd98555d3cdd6abe016768ca5d0e72cfeb Mon Sep 17 00:00:00 2001 From: Harshil Patel Date: Tue, 15 Jun 2021 09:18:23 +0100 Subject: [PATCH 344/344] Black --- nf_core/pipeline-template/bin/check_samplesheet.py | 2 +- tests/test_lint.py | 1 + tests/test_utils.py | 2 +- 3 files changed, 3 insertions(+), 2 deletions(-) diff --git a/nf_core/pipeline-template/bin/check_samplesheet.py b/nf_core/pipeline-template/bin/check_samplesheet.py index 35ae64ee41..abe2cad944 100755 --- a/nf_core/pipeline-template/bin/check_samplesheet.py +++ b/nf_core/pipeline-template/bin/check_samplesheet.py @@ -85,7 +85,7 @@ def check_samplesheet(file_in, file_out): ## Check sample name entries sample, fastq_1, fastq_2 = lspl[: len(HEADER)] - sample = sample.replace(' ', '_') + sample = sample.replace(" ", "_") if not sample: print_error("Sample entry has not been specified!", "Line", line) diff --git a/tests/test_lint.py b/tests/test_lint.py index f51d007aad..d50fa892a1 100644 --- a/tests/test_lint.py +++ b/tests/test_lint.py @@ -213,6 +213,7 @@ def test_sphinx_rst_files(self): test_files_unchanged_fail, ) + # TODO nf-core: Assess and strip out if no longer required for DSL2 # def test_critical_missingfiles_example(self): diff --git a/tests/test_utils.py b/tests/test_utils.py index 08a62f723b..36d533afe4 100644 --- a/tests/test_utils.py +++ b/tests/test_utils.py @@ -78,7 +78,7 @@ def test_load_pipeline_config(self): assert self.pipeline_obj.nf_config["dag.enabled"] == "true" # TODO nf-core: Assess and strip out if no longer required for DSL2 - + # def test_load_conda_env(self): # """Load the pipeline Conda environment.yml file""" # self.pipeline_obj._load_conda_environment()
\n" + for (param in group_params.keySet()) { + summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" + } + summary_section += "