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Add ATAC-Seq data support #129

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heylf opened this issue Jul 11, 2022 · 4 comments
Closed

Add ATAC-Seq data support #129

heylf opened this issue Jul 11, 2022 · 4 comments
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enhancement New feature or request

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@heylf
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heylf commented Jul 11, 2022

Description of feature

This goes hand in hand with the issue for CITE-Seq (#99). Does it make sense to also try to integrate ATAC-Seq data support in the pipeline as well? scATAC-Seq is also supported by Cell-Ranger's 10x. I will test out the pipeline on some real data anyway in a few days.

Yet, since the name is scrnaseq, it might be a bit hard for people to notice that the pipeline can also be used for scATAC-seq data.

@heylf heylf added the enhancement New feature or request label Jul 11, 2022
@grst grst added this to scrnaseq Jul 28, 2022
@grst
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grst commented Sep 5, 2022

I just learned this would require the cellranger-arc pipeline which seems independent of the regular cellranger command?

So we would probably want to build a separate cellranger-arc module and support this as a separate aligner ("cellranger-arc" among "cellranger"/"kallisto"/"alevin-fry")? Any other thoughts about how to best implement this?

@heylf
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heylf commented Sep 5, 2022

I think it's the best way to go for multiome. For scATAC alone, you could use cellranger itself. I wanted to implement the cellranger-arc module and am currently working on it.

@klkeys
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klkeys commented May 19, 2023

For scATAC alone, you could use cellranger itself.
is this still true? it seems like you need cellranger-atac, whereas cellranger-arc handles the multiome data

scATAC data support should work once nf-core/modules#2450 is merged

@klkeys klkeys linked a pull request May 19, 2023 that will close this issue
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@grst
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grst commented Oct 26, 2023

closing in favor of #174

@grst grst closed this as completed Oct 26, 2023
@github-project-automation github-project-automation bot moved this to Done in scrnaseq Oct 26, 2023
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Labels
enhancement New feature or request
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3 participants