diff --git a/CHANGELOG.md b/CHANGELOG.md index b0f862f28..128e9b7b1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,9 @@ and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0. * [#80](https://github.com/nf-core/eager/pull/80) - BWA Index file handling * [#77](https://github.com/nf-core/eager/pull/77) - Lots of documentation updates by [@jfy133](https://github.com/jfy133) +### `Fixed` +* [#84](https://github.com/nf-core/eager/pull/85) - Fix for [Samtools index issues](https://github.com/nf-core/eager/issues/84) + ## [2.0.2] - 2018-11-03 ### `Changed` diff --git a/main.nf b/main.nf index 82e085c91..fbf516106 100644 --- a/main.nf +++ b/main.nf @@ -590,7 +590,7 @@ process bwa { """ bwa aln -t ${task.cpus} $fasta $reads -n ${params.bwaalnn} -l ${params.bwaalnl} -k ${params.bwaalnk} -f "${reads.baseName}.sai" bwa samse -r "@RG\\tID:ILLUMINA-${prefix}\\tSM:${prefix}\\tPL:illumina" $fasta "${reads.baseName}".sai $reads | samtools sort -@ ${task.cpus} -O bam - > "${prefix}".sorted.bam - samtools index -@ ${task.cpus} "${prefix}".sorted.bam + samtools index "${prefix}".sorted.bam """ } @@ -642,7 +642,7 @@ process circularmapper{ bwa samse -r "@RG\\tID:ILLUMINA-${prefix}\\tSM:${prefix}\\tPL:illumina" "${fasta.baseName}_${params.circularextension}.fasta" "${reads.baseName}".sai $reads > tmp.out realignsamfile -e ${params.circularextension} -i tmp.out -r $fasta $filter samtools sort -@ ${task.cpus} -O bam tmp_realigned.bam > "${prefix}".sorted.bam - samtools index -@ ${task.cpus} "${prefix}".sorted.bam + samtools index "${prefix}".sorted.bam """ } @@ -724,12 +724,12 @@ process samtools_filter { """ samtools view -h $bam | tee >(samtools view - -@ ${task.cpus} -f4 -q ${params.bam_mapping_quality_threshold} -o ${prefix}.unmapped.bam) >(samtools view - -@ ${task.cpus} -F4 -q ${params.bam_mapping_quality_threshold} -o ${prefix}.filtered.bam) samtools fastq -tn "${prefix}.unmapped.bam" | gzip > "${prefix}.unmapped.fq.gz" - samtools index -@ ${task.cpus} ${prefix}.filtered.bam + samtools index ${prefix}.filtered.bam """ } else { """ samtools view -h $bam | tee >(samtools view - -@ ${task.cpus} -f4 -q ${params.bam_mapping_quality_threshold} -o ${prefix}.unmapped.bam) >(samtools view - -@ ${task.cpus} -q ${params.bam_mapping_quality_threshold} -o ${prefix}.filtered.bam) - samtools index -@ ${task.cpus} ${prefix}.filtered.bam + samtools index ${prefix}.filtered.bam """ } } @@ -764,14 +764,14 @@ process dedup{ dedup -i $bam $treat_merged -o . -u mv *.log dedup.log samtools sort -@ ${task.cpus} "$prefix"_rmdup.bam -o "$prefix".sorted.bam - samtools index -@ ${task.cpus} "$prefix".sorted.bam + samtools index "$prefix".sorted.bam """ } else { """ dedup -i $bam $treat_merged -o . -u mv *.log dedup.log samtools sort -@ ${task.cpus} "$prefix"_rmdup.bam -o "$prefix".sorted.bam - samtools index -@ ${task.cpus} "$prefix".sorted.bam + samtools index "$prefix".sorted.bam """ } }