Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

extract_od is excessively slow with Nautilus data #85

Open
lenafb opened this issue Sep 17, 2021 · 0 comments
Open

extract_od is excessively slow with Nautilus data #85

lenafb opened this issue Sep 17, 2021 · 0 comments

Comments

@lenafb
Copy link
Contributor

lenafb commented Sep 17, 2021

When analyzing images from the same plate (acquired on different machines) it takes multiSero ~7.5x longer to extract ODs from Nautilus images than Scienion images. Most likely this is due to the fact that multiSero looks for the well before applying masks and particle filter steps, hence WARNING:root:Couldn't find well in {current well} error.

To Reproduce
Steps to reproduce the behavior:
To best reproduce illustrate this behavior, I recommend running multiSero debug configs with the following commands (main difference being path to Scienion data vs path to Nautilus data):

Scienion run:
-e -i "/Volumes/GoogleDrive/Shared drives/compmicro/ELISAarrayReader/images_scienion/2021-08-20-12-09-52-head_to_head_plateII/E_to_H" -o "/Volumes/GoogleDrive/Shared drives/compmicro/ELISAarrayReader/images_scienion/2021-08-20-12-09-52-head_to_head_plateII/E_to_H" -d

Nautilus run:
-e -i "/Volumes/GoogleDrive/Shared drives/compmicro/ELISAarrayReader/images_nautilus/2021-08-20-VLP_RVP_NS1/E_to_H" -o "/Volumes/GoogleDrive/Shared drives/compmicro/ELISAarrayReader/images_nautilus/2021-08-20-VLP_RVP_NS1/E_to_H" -d

Expected behavior
multiSero should be able run extract_od in <5 min.

Operating environment (please complete the following information):

  • OS: Mac, Jetson Nano

Additional context
The strategy I'll pursue is to suppress multiSero's effort to search for the well boundaries, since Nautilus data often does not include well boundaries.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant