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GUI_0.5.py
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import Tkinter as tk
import tkFileDialog as tkfd
import numpy as np
import cv2
import MMCorePy
import PIL.Image, PIL.ImageTk
# Initializing the microscope. ------------------------------
DEVICE = ['Camera', 'DemoCamera', 'DCam']
mmc = MMCorePy.CMMCore()
mmc.enableStderrLog(False)
mmc.enableDebugLog(False)
mmc.loadDevice(*DEVICE)
mmc.initializeDevice(DEVICE[0])
mmc.setCameraDevice(DEVICE[0])
mmc.setProperty(DEVICE[0], 'PixelType', '32bitRGB')
mmc.startContinuousSequenceAcquisition(1)
# OOP ------------------------------------------------------------
class App:
def __init__(self, root, title):
self.root = root
self.root.title(title)
# checkbutton 1 & label 1 & button 1 --------------------
self.var1 = tk.BooleanVar()
self.var1.set(True)
self.label_1 = tk.Label(self.root, text = str((256,256)), font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_1.grid(row = 0, column=1)
self.btn_1 = tk.Button(self.root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.NORMAL, command = self.setbutton_1)
self.btn_1.grid(row = 0, column=2)
self.cbtn_1 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap A', font='12',
variable = self.var1,
disabledforeground = 'black',
state = tk.DISABLED)
self.cbtn_1.grid(row = 0, column = 0, padx = 5)
# checkbutton 2 & label 2 & button 2 --------------------
self.var2 = tk.BooleanVar()
self.label_2 = tk.Label(self.root, text = 'None', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_2.grid(row = 1, column=1)
self.btn_2 = tk.Button(self.root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.DISABLED, command = self.setbutton_2)
self.btn_2.grid(row = 1, column=2)
self.cbtn_2 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap B', font='12',
variable = self.var2,
command = self.enable_2)
self.cbtn_2.grid(row = 1, column = 0, padx = 5)
# checkbutton 3 & label 3 & button 3 --------------------
self.var3 = tk.BooleanVar()
self.label_3 = tk.Label(self.root, text = 'None', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_3.grid(row = 2, column=1)
self.btn_3 = tk.Button(self.root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.DISABLED, command = self.setbutton_3)
self.btn_3.grid(row = 2, column=2)
self.cbtn_3 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap C', font='12',
variable = self.var3,
command = self.enable_3)
self.cbtn_3.grid(row = 2, column = 0, padx = 5)
# checkbutton 2 & label 2 & button 2 --------------------
self.var4 = tk.BooleanVar()
self.label_4 = tk.Label(self.root, text = 'None', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_4.grid(row = 3, column=1)
self.btn_4 = tk.Button(self.root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.DISABLED, command = self.setbutton_4)
self.btn_4.grid(row = 3, column=2)
self.cbtn_4 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap D', font='12',
variable = self.var4,
command = self.enable_4)
self.cbtn_4.grid(row = 3, column = 0, padx = 5)
# real-time coords labels ----------------------------------------
self.word_label = tk.Label(self.root, text = "Position : ",
font = '12', relief = tk.FLAT)
self.word_label.grid(row = 4, column = 0,
sticky=tk.E)
self.cur_label = tk.Label(self.root, font = '12', text = '--',
height = 2, width = 10,
relief = tk.FLAT)
self.cur_label.grid(row = 4, column = 1, padx = 5, sticky=tk.W)
# unset button ----------------------------------------
self.btn_unset = tk.Button(self.root, text='Done setting',
font = '12', fg = 'blue',
height = 2,
command = self.unset)
self.btn_unset.grid(row = 4, column = 2, padx = 5, sticky=tk.W)
# snapshot button ----------------------------------------
self.btn_snapshot = tk.Button(self.root,
text='Snapshot', font='12',
foreground = 'darkgreen',
height = 2,
command = self.snapshot)
self.btn_snapshot.grid(row = 5, column = 0, columnspan = 3,
sticky=tk.W+tk.E+tk.N+tk.S, pady=20, padx = 5)
# canvas --------------------------------------------------
self.canvas = tk.Canvas(self.root, width = 509, height = 509)
self.canvas.grid(column = 4, row = 0, rowspan = 6,
sticky=tk.W+tk.E+tk.N+tk.S,
padx = 5, pady = 10)
self.update()
# mouse stuff --------------------------------------------------
self.in_var = False
self.canvas.bind('<Enter>', self.enter)
self.canvas.bind('<Leave>', self.leave)
self.root.bind('<Motion>', self.motion)
# shutdown --------------------------------------------------
self.root.wm_protocol("WM_DELETE_WINDOW", self.out)
# -------------------------------------------------------
def update(self):
if mmc.getRemainingImageCount() > 0:
rgb32 = mmc.getLastImage()
bgr = rgb32.view(dtype=np.uint8).reshape(
rgb32.shape[0], rgb32.shape[1], 4)[..., :3]
prephoto = bgr
else:
prephoto = cv2.imread('blank.png')
photo = PIL.ImageTk.PhotoImage(image = PIL.Image.fromarray(prephoto))
self.canvas.photo = photo
self.canvas.create_image(0, 0, image = photo, anchor = tk.NW)
self.root.after(1, self.update)
# -------------------------------------------------------
def snapshot(self):
if mmc.getRemainingImageCount() > 0:
rgb32 = mmc.getLastImage()
bgr = rgb32.view(dtype=np.uint8).reshape(
rgb32.shape[0], rgb32.shape[1], 4)[..., :3]
snap = bgr
else:
snap = cv2.imread('blank.png')
output = tkfd.asksaveasfilename(initialdir = "/",
title = "Save JPEG file only",
defaultextension='.jpg',
filetypes = (("JPEG","*.jpg"),
("","*.??")))
cv2.imwrite(output, snap)
# -------------------------------------------------------
def enable(self, whichvar, whichlabel, whichbutton):
if whichvar.get() == True:
whichbutton.config(state=tk.NORMAL)
whichlabel.config(text = str((256,256)))
elif whichvar.get() == False:
whichbutton.config(state=tk.DISABLED)
whichlabel.config(text = 'None')
def enable_2(self):
self.enable(self.var2, self.label_2, self.btn_2)
self.root.config(cursor = 'arrow')
self.root.unbind('<Button-1>')
def enable_3(self):
self.enable(self.var3, self.label_3, self.btn_3)
self.root.config(cursor = 'arrow')
self.root.unbind('<Button-1>')
def enable_4(self):
self.enable(self.var4, self.label_4, self.btn_4)
self.root.config(cursor = 'arrow')
self.root.unbind('<Button-1>')
# -------------------------------------------------------
def setbutton_1(self):
self.root.config(cursor = 'plus')
self.root.bind('<Button-1>', self.click_1)
def setbutton_2(self):
self.root.config(cursor = 'plus')
self.root.bind('<Button-1>', self.click_2)
def setbutton_3(self):
self.root.config(cursor = 'plus')
self.root.bind('<Button-1>', self.click_3)
def setbutton_4(self):
self.root.config(cursor = 'plus')
self.root.bind('<Button-1>', self.click_4)
def unset(self):
self.root.config(cursor = 'arrow')
self.root.unbind('<Button-1>')
# -------------------------------------------------------
def click_1(self, event):
if self.in_var == True:
self.tx, self.ty = event.x, event.y
self.label_1.config(text = '({}, {})'.format(self.tx, self.ty))
def click_2(self, event):
if self.in_var == True:
self.tx, self.ty = event.x, event.y
self.label_2.config(text = '({}, {})'.format(self.tx, self.ty))
def click_3(self, event):
if self.in_var == True:
self.tx, self.ty = event.x, event.y
self.label_3.config(text = '({}, {})'.format(self.tx, self.ty))
def click_4(self, event):
if self.in_var == True:
self.tx, self.ty = event.x, event.y
self.label_4.config(text = '({}, {})'.format(self.tx, self.ty))
# -------------------------------------------------------
def enter(self, event):
self.in_var = True
def leave(self, event):
self.in_var = False
def motion(self, event):
if self.in_var == True:
self.x, self.y = event.x, event.y
self.cur_label.config(text = '({}, {})'.format(self.x, self.y))
if self.in_var == False:
self.cur_label.config(text = '--')
# -------------------------------------------------------
def out(self):
mmc.stopSequenceAcquisition()
self.root.destroy()
# ------------------------------------------------------------
pba = App(tk.Tk(), "The Optical Tweezer Program")
pba.root.mainloop()