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GUI_0.3.py
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import Tkinter as tk
import tkFileDialog as tkfd
import numpy as np
import cv2
import MMCorePy
import PIL.Image, PIL.ImageTk
# Initializing the microscope. ------------------------------
DEVICE = ['Camera', 'DemoCamera', 'DCam']
mmc = MMCorePy.CMMCore()
mmc.enableStderrLog(False)
mmc.enableDebugLog(False)
mmc.loadDevice(*DEVICE)
mmc.initializeDevice(DEVICE[0])
mmc.setCameraDevice(DEVICE[0])
mmc.setProperty(DEVICE[0], 'PixelType', '32bitRGB')
mmc.startContinuousSequenceAcquisition(1)
# ------------------------------------------------------------
class App:
def __init__(self, root, title):
self.root = root
self.root.title(title)
# checkbutton 1 & label 1 & button 1 --------------------
self.var1 = tk.BooleanVar()
self.var1.set(True)
self.label_1 = tk.Label(root, text = 'COORDS', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_1.grid(row = 0, column=1)
self.btn_1 = tk.Button(root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.NORMAL)
self.btn_1.grid(row = 0, column=2)
self.cbtn_1 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap 1', font='12',
variable = self.var1,
state = tk.DISABLED)
self.cbtn_1.grid(row = 0, column = 0)
# checkbutton 2 & label 2 & button 2 --------------------
self.var2 = tk.BooleanVar()
self.label_2 = tk.Label(root, text = 'COORDS', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_2.grid(row = 1, column=1)
self.btn_2 = tk.Button(root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.DISABLED)
self.btn_2.grid(row = 1, column=2)
self.cbtn_2 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap 2', font='12',
variable = self.var2,
command = self.enable_2)
self.cbtn_2.grid(row = 1, column = 0)
# checkbutton 3 & label 3 & button 3 --------------------
self.var3 = tk.BooleanVar()
self.label_3 = tk.Label(root, text = 'COORDS', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_3.grid(row = 2, column=1)
self.btn_3 = tk.Button(root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.DISABLED)
self.btn_3.grid(row = 2, column=2)
self.cbtn_3 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap 3', font='12',
variable = self.var3,
command = self.enable_3)
self.cbtn_3.grid(row = 2, column = 0)
# checkbutton 2 & label 2 & button 2 --------------------
self.var4 = tk.BooleanVar()
self.label_4 = tk.Label(root, text = 'COORDS', font='12',
height = 2, width = 16,
relief = tk.FLAT)
self.label_4.grid(row = 3, column=1)
self.btn_4 = tk.Button(root, text = 'Set', font = '12', fg = 'orange',
height = 1, width = 6,
disabledforeground = 'grey',
state = tk.DISABLED)
self.btn_4.grid(row = 3, column=2)
self.cbtn_4 = tk.Checkbutton(self.root, relief = tk.GROOVE,
height = 2, width = 8,
text='Trap 4', font='12',
variable = self.var4,
command = self.enable_4)
self.cbtn_4.grid(row = 3, column = 0)
# snapshot button ----------------------------------------
self.btn_snapshot = tk.Button(self.root,
text='Snapshot', font='12',
foreground = 'darkgreen',
command = self.snapshot)
self.btn_snapshot.grid(row = 4, column = 0, columnspan = 3,
sticky=tk.W+tk.E+tk.N+tk.S)
# canvas --------------------------------------------------
self.canvas = tk.Canvas(self.root, width = 512, height = 512)
self.canvas.grid(column = 4, row = 0, rowspan = 5)
self.update()
self.root.wm_protocol("WM_DELETE_WINDOW", self.out)
def update(self):
if mmc.getRemainingImageCount() > 0:
rgb32 = mmc.getLastImage()
bgr = rgb32.view(dtype=np.uint8).reshape(
rgb32.shape[0], rgb32.shape[1], 4)[..., :3]
prephoto = bgr
else:
prephoto = cv2.imread('blank.png')
photo = PIL.ImageTk.PhotoImage(image = PIL.Image.fromarray(prephoto))
self.canvas.photo = photo
self.canvas.create_image(0, 0, image = photo, anchor = tk.NW)
self.root.after(1, self.update)
def snapshot(self):
if mmc.getRemainingImageCount() > 0:
rgb32 = mmc.getLastImage()
bgr = rgb32.view(dtype=np.uint8).reshape(
rgb32.shape[0], rgb32.shape[1], 4)[..., :3]
snap = bgr
else:
snap = cv2.imread('blank.png')
output = tkfd.asksaveasfilename(initialdir = "/",
title = "Save JPEG file only",
defaultextension='.jpg',
filetypes = (("JPEG","*.jpg"),
("","*.??")))
cv2.imwrite(output, snap)
def enable(self, whichvar, whichlabel, whichbutton):
if whichvar.get() == True:
whichbutton.config(state=tk.NORMAL)
elif whichvar.get() == False:
whichbutton.config(state=tk.DISABLED)
def enable_2(self):
self.enable(self.var2, self.label_2, self.btn_2)
def enable_3(self):
self.enable(self.var3, self.label_3, self.btn_3)
def enable_4(self):
self.enable(self.var4, self.label_4, self.btn_4)
def out(self):
mmc.stopSequenceAcquisition()
self.root.destroy()
pba = App(tk.Tk(), "The Optical Tweezer Program")
pba.root.mainloop()