ROCker

Accurately detecting functional genes in metagenomes.

System requirements

Ruby, with the restclient and nokogiri packages. To install the required packages execute:
```
$> gem install rest-client
$> gem install nokogiri
$> gem install json
```
R, with the pROC package. To install the required packages, execute:
```
$> R
R> install.packages('pROC');
```
A metagenome simulation software: Grinder.
A local search software: NCBI BLAST+, DIAMOND, or any other software producing a results in the same format of Tabular-BLAST without comments (use the --search-cmd and --makedb-cmd options to execute any other software).
A multiple alignment software: MUSCLE, Clustal Omega or any other software supporting FastA input and output (use the --aligner-cmd option to execute any other software).

Install ROCker using RubyGems:

$> gem install bio-rocker

Or get the source from GitHub:

$> git clone https://github.com/lmrodriguezr/rocker.git
$> ./rocker/bin/rocker

Luis H (Coto) Orellana, Luis M. Rodriguez-R & Konstantinos Konstantinidis, at the Kostas lab.