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visCluster绘制拟时序时,能否添加分组信息 #89

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sdnddr opened this issue Aug 20, 2024 · 2 comments
Open

visCluster绘制拟时序时,能否添加分组信息 #89

sdnddr opened this issue Aug 20, 2024 · 2 comments

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@sdnddr
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sdnddr commented Aug 20, 2024

非常感谢junjun老师的优秀工具!
想请教一个问题:在对接分析monocle3得到的cds文件时,

mat <- pre_pseudotime_matrix(cds_obj = cds, gene_list = genes)
ck <- clusterData(exp = mat, cluster.method = "kmeans", cluster.num = 5)
visCluster(object = ck, plot.type = "both", add.sampleanno = F, markGenes = sample(rownames(mat),30,replace = F))

能否分组展示热图或者在同一张热图中展示分组信息(case与control)?(注:这样做的目的是在某一细胞亚群中,比对两组间特定基因按照时序表达变化的不同。尝试使用mulGroup参数没有得到理想结果),谢谢~

@junjunlab
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发个类似的文献的图看看

@sdnddr
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sdnddr commented Oct 17, 2024

抱歉回复的慢了。没有看到类似的图,是在分析数据的时候这么考虑的

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