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"NNNNN" model kmer in events output #438

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EPKok opened this issue Aug 16, 2018 · 1 comment
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"NNNNN" model kmer in events output #438

EPKok opened this issue Aug 16, 2018 · 1 comment

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@EPKok
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EPKok commented Aug 16, 2018

Hi, referring to the example of events output provided, why is there NNNNN for model_kmer (4th row excluding header)? And why is it that a valid kmer such as ATGGAG can be assigned even if the model kmer is NNNNN?

contig    position  reference_kmer  read_index  strand  event_index  event_level_mean  event_stdv  event_length  model_kmer  model_mean  model_stdv  standardized_level
gi|545778205|gb|U00096.3|:c514859-514401  3         ATGGAG          0           t       16538        89.82             3.746       0.00100       CTCCAT      92.53       2.49        -0.88
gi|545778205|gb|U00096.3|:c514859-514401  3         ATGGAG          0           t       16537        88.89             2.185       0.00100       CTCCAT      92.53       2.49        -1.18
gi|545778205|gb|U00096.3|:c514859-514401  3         ATGGAG          0           t       16536        94.96             2.441       0.00125       CTCCAT      92.53       2.49        0.79
gi|545778205|gb|U00096.3|:c514859-514401  3         ATGGAG          0           t       16535        81.63             2.760       0.00150       NNNNNN      0.00        0.00        inf
gi|545778205|gb|U00096.3|:c514859-514401  7         AGTTAA          0           t       16534        78.96             2.278       0.00075       TTAACT      75.55       3.52        0.79
gi|545778205|gb|U00096.3|:c514859-514401  8         GTTAAT          0           t       16533        98.81             4.001       0.00100       ATTAAC      95.87       3.30        0.72
gi|545778205|gb|U00096.3|:c514859-514401  9         TTAATG          0           t       16532        96.92             1.506       0.00150       CATTAA      95.43       3.32        0.36
gi|545778205|gb|U00096.3|:c514859-514401  10        TAATGG          0           t       16531        70.86             0.402       0.00100       CCATTA      68.99       3.70        0.41
gi|545778205|gb|U00096.3|:c514859-514401  11        AATGGT          0           t       16530        91.24             4.256       0.00175       ACCATT      85.84       2.74        1.60
@jts
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jts commented Aug 16, 2018

Hi,

We have a state in our model that allows us to ignore events that may be sequencing artifacts. When this state is used, we output NNNNNN in the model_kmer column. We recommend these events are ignored in any subsequent analysis.

Jared

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