signatures and plotheat.SCINA function

[februaryfang]

I use SCINA to annotate my single-cell data. I met two problems.

1. my signatures info is like this:
   marker <- list(
   c("CD8A", "CD8B"), c("CD4", "IL7R"), c("KLRF1", "FGFBP2"), c("FOXP3", "IL2RA"),
   c("MKI67", "STMN1"), c("CD79A", "CD79B"), c("MZB1", "JCHAIN"), c("CA4", "IGFBP5"),
   c("HES4", "ADAMTS9"), c("CRHBP", "AKAP12"), c("ACTA2", "RGS5"), c("APOA1", "HP"),
   c("EPCAM", "KRT7"), c("C1QC", "C1QA"), c("LILRA4", "IL3RA"),
   c("FCN1", "G0S2"), c("CD1C", "CLEC9A")
   )
   names(marker) <- c("CD8T-NKT", "CD4 T", "NK", "CD4 Trg",
   "Cycling T", "B", "Plasma", "Endothelial-1", "Endothelial-2",
   "Normal-like Endothelial", "Stellate cell", "Hepatocyte",
   "Cholangiocyte", "Macrophage", "pDC",
   "Monocyte", "DC")
   when I run this commamd line :
   results = SCINA(mtx, marker, max_iter = 100, convergence_n = 10, convergence_rate = 0.999,
   sensitivity_cutoff = 0.9, rm_overlap=TRUE, allow_unknown=TRUE, log_file='marker.log')
   Then I got the results like this:

   table(results$cell_labels)
   unknown
   95520

When I remove "Monocyte" and "DC" genes in signatures , I got the results like this :
> table(results1$cell_labels)
B CD4 T CD4 Trg
2207 14380 3212
CD8T-NKT Cholangiocyte Cycling T
13438 869 1195
Endothelial-1 Endothelial-2 Hepatocyte
5060 2641 4366
Macrophage NK Normal-like Endothelial
8714 7647 2748
pDC Plasma Stellate cell
2951 1709 1247
unknown
23136
What was the reason for that?

2.my single-cell data has 9.5W cells. I cannot got a heatmap plot by plotheat.SCINA function. There was no error message, it just didn't work.