diff --git a/tests/integration/check_tests.rs b/tests/integration/check_tests.rs
index 8bb0ddde268f7a..f5af5803c47eaa 100644
--- a/tests/integration/check_tests.rs
+++ b/tests/integration/check_tests.rs
@@ -215,7 +215,6 @@ fn typecheck_declarations_unstable() {
   let args = vec![
     "test".to_string(),
     "--doc".to_string(),
-    "--unstable".to_string(),
     util::root_path()
       .join("cli/tsc/dts/lib.deno.unstable.d.ts")
       .to_string_lossy()
diff --git a/tests/integration/run_tests.rs b/tests/integration/run_tests.rs
index a75ca4c1545ad7..e18428575f4380 100644
--- a/tests/integration/run_tests.rs
+++ b/tests/integration/run_tests.rs
@@ -488,9 +488,8 @@ itest!(_088_dynamic_import_already_evaluating {
   output: "run/088_dynamic_import_already_evaluating.ts.out",
 });
 
-// TODO(bartlomieju): remove --unstable once Deno.Command is stabilized
 itest!(_089_run_allow_list {
-  args: "run --unstable --allow-run=curl run/089_run_allow_list.ts",
+  args: "run --allow-run=curl run/089_run_allow_list.ts",
   envs: vec![
     ("LD_LIBRARY_PATH".to_string(), "".to_string()),
     ("DYLD_FALLBACK_LIBRARY_PATH".to_string(), "".to_string())
@@ -2540,14 +2539,12 @@ mod permissions {
   use test_util::itest;
   use util::TestContext;
 
-  // TODO(bartlomieju): remove --unstable once Deno.Command is stabilized
   #[test]
   fn with_allow() {
     for permission in &util::PERMISSION_VARIANTS {
       let status = util::deno_cmd()
         .current_dir(util::testdata_path())
         .arg("run")
-        .arg("--unstable")
         .arg(format!("--allow-{permission}"))
         .arg("run/permission_test.ts")
         .arg(format!("{permission}Required"))
@@ -2559,13 +2556,12 @@ mod permissions {
     }
   }
 
-  // TODO(bartlomieju): remove --unstable once Deno.Command is stabilized
   #[test]
   fn without_allow() {
     for permission in &util::PERMISSION_VARIANTS {
       let (_, err) = util::run_and_collect_output(
         false,
-        &format!("run --unstable run/permission_test.ts {permission}Required"),
+        &format!("run run/permission_test.ts {permission}Required"),
         None,
         None,
         false,
@@ -3019,7 +3015,7 @@ mod permissions {
   fn _066_prompt() {
     TestContext::default()
       .new_command()
-      .args_vec(["run", "--quiet", "--unstable", "run/066_prompt.ts"])
+      .args_vec(["run", "--quiet", "run/066_prompt.ts"])
       .with_pty(|mut console| {
         console.expect("What is your name? Jane Doe");
         console.write_line_raw("");
@@ -3575,7 +3571,6 @@ fn deno_no_prompt_environment_variable() {
   let output = util::deno_cmd()
     .current_dir(util::testdata_path())
     .arg("run")
-    .arg("--unstable")
     .arg("run/no_prompt.ts")
     .env("DENO_NO_PROMPT", "1")
     .spawn()
@@ -4089,7 +4084,6 @@ async fn http2_request_url() {
   let mut child = util::deno_cmd()
     .current_dir(util::testdata_path())
     .arg("run")
-    .arg("--unstable")
     .arg("--quiet")
     .arg("--allow-net")
     .arg("--allow-read")
@@ -4384,7 +4378,6 @@ async fn websocket_server_multi_field_connection_header() {
   let root_ca = util::testdata_path().join("tls/RootCA.pem");
   let mut child = util::deno_cmd()
     .arg("run")
-    .arg("--unstable")
     .arg("--allow-net")
     .arg("--cert")
     .arg(root_ca)
@@ -4438,7 +4431,6 @@ async fn websocket_server_idletimeout() {
   let root_ca = util::testdata_path().join("tls/RootCA.pem");
   let mut child = util::deno_cmd()
     .arg("run")
-    .arg("--unstable")
     .arg("--allow-net")
     .arg("--cert")
     .arg(root_ca)
@@ -5089,7 +5081,6 @@ async fn listen_tls_alpn() {
   let mut child = util::deno_cmd()
     .current_dir(util::testdata_path())
     .arg("run")
-    .arg("--unstable")
     .arg("--quiet")
     .arg("--allow-net")
     .arg("--allow-read")
@@ -5143,7 +5134,6 @@ async fn listen_tls_alpn_fail() {
   let mut child = util::deno_cmd()
     .current_dir(util::testdata_path())
     .arg("run")
-    .arg("--unstable")
     .arg("--quiet")
     .arg("--allow-net")
     .arg("--allow-read")
diff --git a/tests/integration/watcher_tests.rs b/tests/integration/watcher_tests.rs
index 91ac5611f75bf9..2590e79d6fd8fb 100644
--- a/tests/integration/watcher_tests.rs
+++ b/tests/integration/watcher_tests.rs
@@ -499,7 +499,6 @@ async fn run_watch_no_dynamic() {
     .current_dir(t.path())
     .arg("run")
     .arg("--watch")
-    .arg("--unstable")
     .arg("-L")
     .arg("debug")
     .arg(&file_to_watch)
@@ -626,7 +625,6 @@ async fn run_watch_external_watch_files() {
     .arg(watch_arg)
     .arg("-L")
     .arg("debug")
-    .arg("--unstable")
     .arg(&file_to_watch)
     .env("NO_COLOR", "1")
     .piped_output()
@@ -671,7 +669,6 @@ async fn run_watch_load_unload_events() {
     .current_dir(t.path())
     .arg("run")
     .arg("--watch")
-    .arg("--unstable")
     .arg("-L")
     .arg("debug")
     .arg(&file_to_watch)
@@ -723,7 +720,6 @@ async fn run_watch_not_exit() {
     .current_dir(t.path())
     .arg("run")
     .arg("--watch")
-    .arg("--unstable")
     .arg("-L")
     .arg("debug")
     .arg(&file_to_watch)
@@ -873,7 +869,6 @@ async fn test_watch_basic() {
     .current_dir(t.path())
     .arg("test")
     .arg("--watch")
-    .arg("--unstable")
     .arg("--no-check")
     .arg(t.path())
     .env("NO_COLOR", "1")
@@ -1029,7 +1024,6 @@ async fn test_watch_doc() {
     .arg("test")
     .arg("--watch")
     .arg("--doc")
-    .arg("--unstable")
     .arg(t.path())
     .env("NO_COLOR", "1")
     .piped_output()
@@ -1407,7 +1401,6 @@ async fn run_watch_dynamic_imports() {
     .current_dir(t.path())
     .arg("run")
     .arg("--watch")
-    .arg("--unstable")
     .arg("--allow-read")
     .arg("-L")
     .arg("debug")
diff --git a/tests/node_compat/test_runner.rs b/tests/node_compat/test_runner.rs
index 759dd5d3e96f4d..15749ca7fd0dc2 100644
--- a/tests/node_compat/test_runner.rs
+++ b/tests/node_compat/test_runner.rs
@@ -14,7 +14,6 @@ fn node_compat_tests() {
     .arg("--config")
     .arg(deno_config_path())
     .arg("--no-lock")
-    .arg("--unstable")
     .arg("-A")
     .arg(util::tests_path().join("node_compat/test.ts"))
     .spawn()
diff --git a/tests/unit/README.md b/tests/unit/README.md
index af31c08fcc966f..549a9d3caa6afe 100644
--- a/tests/unit/README.md
+++ b/tests/unit/README.md
@@ -30,10 +30,10 @@ There are two ways to run `unit_test_runner.ts`:
 
 ```sh
 # Run all tests.
-cargo run --bin deno -- test --allow-all --unstable --location=http://js-unit-tests/foo/bar cli/tests/unit/
+cargo run --bin deno -- test --allow-all --location=http://js-unit-tests/foo/bar cli/tests/unit/
 
 # Run a specific test module
-cargo run --bin deno -- test --allow-all --unstable --location=http://js-unit-tests/foo/bar cli/tests/unit/files_test.ts
+cargo run --bin deno -- test --allow-all --location=http://js-unit-tests/foo/bar cli/tests/unit/files_test.ts
 ```
 
 ### Http server
diff --git a/tests/unit/command_test.ts b/tests/unit/command_test.ts
index 6352e1fc7b38da..c11761fe72263e 100644
--- a/tests/unit/command_test.ts
+++ b/tests/unit/command_test.ts
@@ -528,7 +528,7 @@ Deno.test(
   },
   async function commandFailedWithSignal() {
     const output = await new Deno.Command(Deno.execPath(), {
-      args: ["eval", "--unstable", "Deno.kill(Deno.pid, 'SIGKILL')"],
+      args: ["eval", "Deno.kill(Deno.pid, 'SIGKILL')"],
     }).output();
     assertEquals(output.success, false);
     if (Deno.build.os === "windows") {
@@ -547,7 +547,7 @@ Deno.test(
   },
   function commandSyncFailedWithSignal() {
     const output = new Deno.Command(Deno.execPath(), {
-      args: ["eval", "--unstable", "Deno.kill(Deno.pid, 'SIGKILL')"],
+      args: ["eval", "Deno.kill(Deno.pid, 'SIGKILL')"],
     }).outputSync();
     assertEquals(output.success, false);
     if (Deno.build.os === "windows") {
@@ -835,7 +835,7 @@ Deno.test(
 const command = await new Deno.Command(Deno.execPath(), {
   cwd: Deno.args[0],
   stdout: "piped",
-  args: ["run", "-A", "--unstable", Deno.args[1]],
+  args: ["run", "-A", Deno.args[1]],
 });
 const child = command.spawn();
 const readable = child.stdout.pipeThrough(new TextDecoderStream());
@@ -877,7 +877,7 @@ setInterval(() => {
       Deno.execPath(),
       {
         cwd,
-        args: ["run", "-A", "--unstable", programFile, cwd, childProgramFile],
+        args: ["run", "-A", programFile, cwd, childProgramFile],
       },
     ).output();
 
diff --git a/tests/unit/files_test.ts b/tests/unit/files_test.ts
index b39b5f417a29ce..c7939710957c8c 100644
--- a/tests/unit/files_test.ts
+++ b/tests/unit/files_test.ts
@@ -929,7 +929,7 @@ function runFlockTestProcess(opts: { exclusive: boolean; sync: boolean }) {
 `;
 
   const process = new Deno.Command(Deno.execPath(), {
-    args: ["eval", "--unstable", scriptText],
+    args: ["eval", scriptText],
     stdin: "piped",
     stdout: "piped",
     stderr: "null",
diff --git a/tests/unit/os_test.ts b/tests/unit/os_test.ts
index 9503f75d12e03b..52aa2ce770a232 100644
--- a/tests/unit/os_test.ts
+++ b/tests/unit/os_test.ts
@@ -172,7 +172,7 @@ Deno.test(
   async function osPpidIsEqualToPidOfParentProcess() {
     const decoder = new TextDecoder();
     const { stdout } = await new Deno.Command(Deno.execPath(), {
-      args: ["eval", "-p", "--unstable", "Deno.ppid"],
+      args: ["eval", "-p", "Deno.ppid"],
       env: { NO_COLOR: "true" },
     }).output();
 
diff --git a/tests/unit/signal_test.ts b/tests/unit/signal_test.ts
index 0c0676428a30d5..65b5ba78e12b0e 100644
--- a/tests/unit/signal_test.ts
+++ b/tests/unit/signal_test.ts
@@ -209,7 +209,6 @@ Deno.test(
     const { code } = await new Deno.Command(Deno.execPath(), {
       args: [
         "eval",
-        "--unstable",
         "Deno.addSignalListener('SIGINT', () => {})",
       ],
     }).output();
diff --git a/tests/unit_node/_test_utils.ts b/tests/unit_node/_test_utils.ts
index d9dd4e5d59d220..c451ccd84b4e0a 100644
--- a/tests/unit_node/_test_utils.ts
+++ b/tests/unit_node/_test_utils.ts
@@ -21,7 +21,6 @@ export async function assertCallbackErrorUncaught(
   const p = new Deno.Command(Deno.execPath(), {
     args: [
       "eval",
-      "--unstable",
       `${prelude ?? ""}
   
         ${invocation}(err) => {
diff --git a/tests/unit_node/child_process_test.ts b/tests/unit_node/child_process_test.ts
index d613d298944fc5..7e6977740ebbed 100644
--- a/tests/unit_node/child_process_test.ts
+++ b/tests/unit_node/child_process_test.ts
@@ -528,7 +528,6 @@ Deno.test({
     const childProcess = spawn(Deno.execPath(), [
       "run",
       "-A",
-      "--unstable",
       script,
     ]);
     const deferred = Promise.withResolvers<void>();
diff --git a/tests/unit_node/process_test.ts b/tests/unit_node/process_test.ts
index 579b51116704f2..944686a87b9a18 100644
--- a/tests/unit_node/process_test.ts
+++ b/tests/unit_node/process_test.ts
@@ -173,7 +173,6 @@ Deno.test({
       args: [
         "run",
         "--quiet",
-        "--unstable",
         "./testdata/process_exit.ts",
       ],
       cwd,
@@ -895,7 +894,6 @@ Deno.test({
       args: [
         "run",
         "--quiet",
-        "--unstable",
         "./testdata/process_exit2.ts",
       ],
       cwd: testDir,
@@ -914,7 +912,6 @@ Deno.test({
       args: [
         "run",
         "--quiet",
-        "--unstable",
         "./testdata/process_really_exit.ts",
       ],
       cwd: testDir,